Chaetomium cupreum

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Chaetomium cupreum
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Eukaryota
Kingdom: Fungi
Division: Ascomycota
Class: Sordariomycetes
Order: Sordariales
Family: Chaetomiaceae
Genus: Chaetomium
Species:
C. cupreum
Binomial name
Chaetomium cupreum
L.M Ames (1949)
Synonyms
  • Chaetomium trilaterale var. cupreumL.M Ames (1973)

Chaetomium cupreum is a fungus in the family Chaetomiaceae. It is able to decay in manufactured cellulosic materials, [1] and is known to antagonize a wide range of soil microorganisms.[ citation needed ] This species is component of the biocontrol agent, Ketomium, a commercial biofungicide. [2] It has also been investigated for use in the production of natural dyes. Chaetomium cupreum is mesophilic and known to occur in harsh environments and can rapidly colonize organic substrates in soil. [3] Laboratory cultures of C. cupreum can be propagated on a range of common growth media including potato dextrose at ambient or higher than ambient temperature producing cottony white colonies with a reddish reverse. [1] [4]

Contents

History

Chaetomium cupreum was described by Lawrence Marion Ames in 1949 as part of a military effort to identify the organisms responsible for the biodeterioration. [1] During this project, Ames documented 9 novel Chaetomium species including the culture Ames described as C. cupreum which was sent to him by Paul Marsh of the U.S Department of Agriculture from deteriorating material collected in the Panama Canal Zone. [5] Ames selected the species epithet "cupreum" based on the copper coloration of the pigments produced by the fungus. [5] A second sample was obtained by G.W Martin in Guadalcanal. Both strains were isolated from rotting clothing, tenting, mattresses and equipment. [1]

Description

The cell wall of C. cupreum is largely composed of chitin and glucan, which is reflected in the large number of acquired genes encoding class V chitin synthase and glucan synthase found in the C. cupreum cDNA. [4] The vegetative mycelium is profusely branched, septate and multicellular; the mycelial cells are multinucleate. [6] The species is distinguished from other Chaetomium species by a high frequency of boat-shaped ascospores and copper coloured terminal hairs. [1] [7] The fruiting bodies occur on the surface of the substratum and are attached by undifferentiated rhizoids. [5] [6] The perithecia of C. cupreum are ovate in shape and copper colored with dimensions of 110–120 x 120–130 μm. [5] The presence of long, thin hairs on the outer surface of the perithecium is a characteristic feature of Chaetomium (Gr. χαίτη = long hair). [6] In C. cupreum, these hairs are numerous, thin, septate lateral hairs with a base 3.0–3.5 μm in diameter. Hairs at the apex of the perithecium are rigid, septate, 4.5–6.0 μm in diameter with 1–2 spirals. [1] [5] [7] The apical hairs are covered with small copper coloured granules whose pigment is soluble in alcohol, ether, cellosolve, xylol but insoluble in water. [5] Club-shaped asci measuring 38 × 13 μm develop in clusters n the interior, basal part of the perithecium. [1] [6] Each ascus contains 8 reddish ascospores that are boat shaped with dimensions of 10.0 × 5.5μm. [3] [8] The walls of the asci are mucilaginous and disintegrate, causing the ascospores to remain inside the perithecium at maturity, embedded in mucilaginous jelly. The ascospores and the mucilaginous matrix form a paste that is extruded through the apical opening in the perithecium producing "cirrhi" resembling toothpaste squeezed out from a toothpaste tube. [5] [6] Chaetomium cupreum is intermediate between the species: C. trilaterale Chivers and C. aureum Chivers. C. aureum and C. cupreum both produce conspicuous cirrhi while C. trilaterale does not. The ascospores of C. cupreum are similar shape but larger than C. aureum. The pigment produced by C. trilaterale in agar cultures is water-soluble while the granules produced on C. cupreum are insoluble. [5]

Reproduction

Chaetomium cupreum is known only as a sexually reproducing species and no asexual form has been reported. Ames originally reported C. cupreum to possess a homothallic mating system but this was later contradicted by Tveit in 1955 who determined the species to be heterothallic. [9] Sexual reproduction in C. cupreum involves the formation of ascogonia arising as lateral outgrowths of the vegetative mycelium. In early developmental stages, the ascogonia are coiled and coenocytic with septa forming as the ascogonia mature. The terminal cell of each ascogonium will become a long trichogyne which functions as the receptive organ. Male reproductive structures, antheridia are commonly absent in Chaetomium. [6]

Metabolism

The metabolism of C. cupreum is complex. In an Expressed Sequence Tag (EST) study conducted by Zhang and Yang in 2007 C. cupreum demonstrated a diverse expression of genes related to metabolic pathways. [4] In their study the most represented metabolic pathway was glycolysis demonstrating its importance in mycelia cell metabolism. The second most represented category was porphyrin and chlorophyll metabolism, the fungi cannot produce chlorophyll but they have a heme biosynthetic pathway. Genes encoding coproporphyrinogen oxidase, an essential enzyme in the heme biosynthetic pathway were found as well as genes associated with the electron transport chain and oxidative phosphorylation. The citric acid cycle also has a role in its energy metabolism with 18% of metabolic genes relating to TCA cycle function. Saccharide metabolism associated genes were also found for the metabolism of: galactose, fructose, mannose, sucrose, starch, nucleotide sugars, amino sugars, as well as glycoprotein and peptide-protein biosynthesis. Many genes have been identified in this species that support protein biosynthesis and proteolytic systems including: glutamate, methionine and tryptophan metabolism; phenylalanine, valine, leucine and isoleucine degradation; valine, leucine, isoleucine, tyrosine and tryptophan biosynthesis. [4] Proteases produced by C. cupreum are involved in pathogen cell wall breakdown and contribute to its biocontrol activity. Biotechnological interest in C. cupreum is related to its production of cellulase and laccase. [10] [11] [12] C. cupreum is able to degrade catechin. [13]

Biotechnology

Agricultural interest in C. cupreum has arisen due to the ability of some strains to suppress infections by plant pathogens. [14] [15] [16] The biocontrol capacity of C.cupreum has been attributed to the production of antifungal metabolites, release of hydrolases, mycoparasitism and competition for nutrients and space. [15] Chaetomium cupreum produces a diverse set of hydrolytic enzymes making it a strong biodegrader and substrate colonizer as a result of its large secretory potential and metabolic versatility. [4] EST analysis of C. cupreum revealed several candidate biocontrol genes related to: cell-wall degradation, [17] proteolytic function, antifungal metabolite production and production of substances that enhance plant disease resistance. [15]

Chaetomium cupreum has genes encoding cell wall hydrolases including: β 1–3 exoglucanase, endoglucanase IV, β glucosidase 5 and 6, and chitinase. β 1–3 exoglucanase, [18] endoglucanase IV and β glucosidases are major lytic enzymes targeting the fungal cell wall responsible for breaking down β-1,3-glucans. These and other hydrolases targeting fungal cell wall components function synergistically [4] and are presumed to play an important role in mycoparasitism. [15] [16] [19] β-1,3-glucan binding protein present in C. cupreum bind specifically to β-1,3-glucan and lipoteichoic acids in the cell wall of pathogens causing aggregation of the invading fungi for host and biocontrol fungi cell recognition and protection. The induction of plant resistance involves xylanases, xylanase genes are found in C. cupreum. [20] The destruction of nascent chitin of pathogens generates oligosaccharides containing GlcNAC which elicits a general antifungal response from C. cupreum. [21] C. cupreum also produces subtilisin-like serine protease and aspartic proteinases found in C. cupreum that contribute to cell wall degradation and deactivation of pathogen enzymes. [4]

Antifungal metabolites

Chaetomium cupreum produces a range of antifungal metabolites including polyketide synthase, terpenes, chetomin, rotiorinols A-C, "multidrug resistance protein", isopenicillin N synthase and related dioxygenases some of which have been investigated for pharmaceutical use. [4] [22] A beta-lactamase-like major facilitator in C. cupreum provides tolerance to toxic compounds, such as fungicides. [23] Several pigments produced by this species including rotiorinols A & C, (-)-rotiorin and rubrorotiorin have been shown to exhibit antifungal activity against the pathogenic yeast, Candida albicans . [24] Pigment produced by C. cupreum has in vitro antagonistic activity against the phytopathogenic bacterium, Ralstonia solanacearum.[ citation needed ]

Commercial use

Chaetomium cupreum is able to antagonize a wide set of plant pathogens including Magnaporthe grisea, Rhizoctonia solani and Cochliobolus lunatus. [15] [16] Registered and commercially available as "Ketomium" mycofungicide, Ketomium is a biofungicide comprising 22-strains of C. cupreum and C. globosum for use in disease control of various pathogens. [2] The product has been implementation as a biocontrol agent in a number of geographic localities including China, Philippines, Russia, Vietnam and Thailand. [2] [25] Ketomium has been shown to produces an endurable protection against pathogens including: Phytophthora palmivora, Phytophthora nicotianae, Phytophthora cactorum, Fusarium oxysporum, and Athelia rolfsii . [2] These phytopathogens are known to infect economically important plants such as durian, black peppers, tangerine, strawberry, tomato, corn and pomelo. [2] [25]

Pigments

The extracellular pigment produced by C. cupreum is influenced by environmental factors such as pH in which low pH causes the pigments to turn yellow and high pH restores the characteristic red colour.[ citation needed ] In a photoresponse study researchers investigated the effect of variable wavelengths of visible light on the production of pigments. [26] C. cupreum biomass and pigment production were variable depending on the wavelength of light used during the 7 day incubation period. The white colonies produced ascospores and a deep red, water-soluble reverse pigment. Incubation in white light lead to the largest colony diameter while green light lead to the greatest pigment production. The varying concentrations suggests pigment loss, possibly explained by nutrient depletion induced enzymatic breakdown of pigments – a common phenomena where secondary metabolites are degraded by enzymes. [27]

Related Research Articles

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<i>Sordaria fimicola</i> Species of fungus

Sordaria fimicola is a species of microscopic fungus. It is commonly found in the feces of herbivores. Sordaria fimicola is often used in introductory biology and mycology labs because it is easy to grow on nutrient agar in dish cultures. The genus Sordaria, closely related to Neurospora and Podospora, is a member of the large class Sordariomycetes, or flask-fungi. The natural habitat of the three species of Sordaria that have been the principal subjects in genetic studies is dung of herbivorous animals. The species S. fimicola is common and worldwide in distribution. The species of Sordaria are similar morphologically, producing black perithecia containing asci with eight dark ascospores in a linear arrangement. These species share a number of characteristics that are advantageous for genetic studies. They all have a short life cycle, usually 7–12 days, and are easily grown in culture. Most species are self-fertile and each strain is isogenic. All kinds of mutants are easily induced and readily obtainable with particular ascospore color mutants. These visual mutants aid in tetrad analysis, especially in analysis of intragenic recombination.

<span class="mw-page-title-main">Anidulafungin</span> Antifungal medication

Anidulafungin (INN) is a semisynthetic echinocandin used as an antifungal drug. It was previously known as LY303366. It may also have application in treating invasive Aspergillus infection when used in combination with voriconazole. It is a member of the class of antifungal drugs known as the echinocandins; its mechanism of action is by inhibition of (1→3)-β-D-glucan synthase, an enzyme important to the synthesis of the fungal cell wall.

<i>Aspergillus nidulans</i> Species of fungus

Aspergillus nidulans is one of many species of filamentous fungi in the phylum Ascomycota. It has been an important research organism for studying eukaryotic cell biology for over 50 years, being used to study a wide range of subjects including recombination, DNA repair, mutation, cell cycle control, tubulin, chromatin, nucleokinesis, pathogenesis, metabolism, and experimental evolution. It is one of the few species in its genus able to form sexual spores through meiosis, allowing crossing of strains in the laboratory. A. nidulans is a homothallic fungus, meaning it is able to self-fertilize and form fruiting bodies in the absence of a mating partner. It has septate hyphae with a woolly colony texture and white mycelia. The green colour of wild-type colonies is due to pigmentation of the spores, while mutations in the pigmentation pathway can produce other spore colours.

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<i>Chaetomium</i> Genus of fungi

Chaetomium is a genus of fungi in the Chaetomiaceae family. It is a dematiaceous (dark-walled) mold normally found in soil, air, cellulose and plant debris. According to the Dictionary of the Fungi, there are about 95 species in the widespread genus.

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<i>Chaetomium globosum</i> Species of fungus

Chaetomium globosum is a well-known mesophilic member of the mold family Chaetomiaceae. It is a saprophytic fungus that primarily resides on plants, soil, straw, and dung. Endophytic C. globosum assists in cellulose decomposition of plant cells. They are found in habitats ranging from forest plants to mountain soils across various biomes. C. globosum colonies can also be found indoors and on wooden products.

Thielavia subthermophila is a ubiquitous, filamentous fungus that is a member of the phylum Ascomycota and order Sordariales. Known to be found on plants of arid environments, it is an endophyte with thermophilic properties, and possesses dense, pigmented mycelium. Thielavia subthermophila has rarely been identified as a human pathogen, with a small number of clinical cases including ocular and brain infections. For treatment, antifungal drugs such as amphotericin B have been used topically or intravenously, depending upon the condition.

Chaetomium atrobrunneum is a darkly pigmented mould affiliated with the fungal division, Ascomycota. This species is predominantly saprotrophic, although it has been known to infect animals including humans, showing a proclivity for the tissues of the central nervous system. Chaetomium atrobrunneum was described in 1949 from a mouldy military mattress cover obtained from the island of Guadalcanal.

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Botryotrichum murorum is a common soil and indoor fungus resembling members of the genus Chaetomium. The fungus has no known asexual state, and unlike many related fungi, is intolerant of high heat exhibiting limited growth when incubated at temperatures over 35 °C. In rare cases, the fungus is an opportunistic pathogen of marine animals and humans causing cutaneous and subcutaneous infection.

<i>Chaetomium elatum</i> Species of fungus

Chaetomium elatum is a very common and widely distributed saprotrophic fungus of the Chaetomiaceae family of molds which has been found to grow on many different substances all over the world. It was first established by Gustav Kunze after he observed it growing on dead leaves. Its defining features that distinguish it from other Chaetomium species are its extremely coarse terminal hairs and the lemon-shaped morphology of its ascospores. It produces many metabolites with potential biotechnology uses including one with promise against the rice blast disease fungus, Magnaporthe grisea. It shows very little pathogenic ability causing confirmed disease in only a few plant species.

Botryotrichum piluliferum is a fungal species first identified in 1885 by Saccardo and Marchal. It was discovered to be the asexual state of a member of the ascomycete genus, Chaetomium. The name B. piluliferum now applies to the fungus in all its states. B. piluliferum has been found worldwide in a wide range of habitats such as animal dung and vegetation. The colonies of this fungus start off white and grow rapidly to a brown colour. The conidia are smooth and white. B. piluliferum grows optimally at a temperature of 25-30 °C and a pH of 5.5.

Arcopilus aureus is a plant and soil fungus in the genus Arcopilus. It was first identified by A. H. Chivers in 1912, who named it Chaetomium aureum. It was later transferred to the genus Arcopilus by Wang and colleagues. The fungus has recently been recognized to have industrial use for the production of the metabolites resveratrol. and sclerotiorin Additionally, A. aureus has high lead tolerance and clearance, suggesting a potential role in environmental biotechnology.

Cercophora areolata is a member of the Ascomycota division, and is grouped into the Lasiosphaeriaceae family based on morphology. C. areolata is a coprophilous fungus that has been most recently isolated from porcupine dung. Defining features of C. areolata include: 1) ovoid-conical, glabrous ascomata, 2) black, carbonaceous, areolate peridium and 3) clavate-shaped, single-walled asci. From studies on C. areolata, this fungus produces multiple antifungal compounds, which inhibit other competitor fungi.

Chaetomium perlucidum is a neurotropic dematiaceous fungus that is naturally found in the soil, including in agricultural soil, and in the stems of dead plants. The fungus can also be found on the feathers of birds, manure, seeds, and even paper. It is able to thrive at temperatures of 35 and 42 °C.

References

  1. 1 2 3 4 5 6 7 Ames, L. M. (1 November 1949). "New Cellulose Destroying Fungi Isolated from Military Material and Equipment". Mycologia. 41 (6): 637–648. doi:10.2307/3755020. JSTOR   3755020.
  2. 1 2 3 4 5 Soytong, K; Kanokmedhakul, S; Kuknogviriyapa, V; Isobe, M (2001). "Application of Chaetomium species (Ketomium) as a new broad spectrum biological fungicide for plant disease control: A review article". Fungal Diversity. 7: 1–15.
  3. 1 2 Millner, P. D.; Motta, J. J.; Lentz, P. L. (1 July 1977). "Ascospores, Germ Pores, Ultrastructure, and Thermophilism of Chaetomium". Mycologia. 69 (4): 720–733. doi:10.2307/3758862. JSTOR   3758862.
  4. 1 2 3 4 5 6 7 8 Zhang, HaiYan; Yang, Qian (13 January 2007). "Expressed sequence tags-based identification of genes in the biocontrol agent Chaetomium cupreum". Applied Microbiology and Biotechnology. 74 (3): 650–658. doi:10.1007/s00253-006-0701-2. ISSN   0175-7598. PMID   17221201. S2CID   12948208.
  5. 1 2 3 4 5 6 7 8 Ames, L.M (1961). A Monograph of the Chaetomiaceae. 3301 Lehre Germany: Verlag von J.Cramer. pp. 2, 3, 9, 21.{{cite book}}: CS1 maint: location (link)
  6. 1 2 3 4 5 6 Singh, Pande J. (2008). Text Book of Botony Diversity of Microbes and Cryptogams. Gangotri, India: Rastogi Publications. pp. 308–310. ISBN   978-81-7133-889-4.
  7. 1 2 Watanabe, Tsuneo (2010). Pictorial Atlas of Soil and Seed Fungi: Morphologies of Cultured Fungi and Key to Species. Danvers MA: CRC Press. pp. 100–101.
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  11. ↑ Mimura, S.; Rao, U.; Yoshino, S.; Kato, M.; Tsukagoshi, N. (1 January 1999). "Depression of the xylanase-encoding cgxA gene of Chaetomium gracile in Aspergillus nidulans". Microbiological Research. 153 (4): 369–376. doi:10.1016/S0944-5013(99)80052-4. ISSN   0944-5013. PMID   10052158.
  12. ↑ Chefetz, B (September 1998). "Purification and characterization of laccase from Chaetomium thermophilium and its role in humification". Applied and Environmental Microbiology. 64 (9): 3175–9. Bibcode:1998ApEnM..64.3175C. doi:10.1128/AEM.64.9.3175-3179.1998. PMC   106706 . PMID   9726856.
  13. ↑ Sambandam, T.; Mahadevan, A. (1993). "Degradation of catechin and purification and partial characterization of catechin oxygenase from Chaetomium cupreum". World Journal of Microbiology & Biotechnology. 9 (1): 37–44. doi:10.1007/BF00656513. PMID   24419836. S2CID   1257624.
  14. ↑ Manandhar, J. B.; Thapliyal, P. N.; Cavanaugh, K. J.; Sinclair, J. B. (1 May 1987). "Interaction between pathogenic and saprobic fungi isolated from soybean roots and seeds". Mycopathologia. 98 (2): 69–75. doi:10.1007/BF00437291. ISSN   0301-486X. S2CID   31795094.
  15. 1 2 3 4 5 Soytong, K (2003). Application of a new broad spectrum biological fungicide for environmental plant protection. In: Yang Q. Harbin: Heilongjang Science and Technology Press. pp. 70–85.
  16. 1 2 3 Soytong, Kasem, N. Jindawong, and Q. Yang. "Evaluation of Chaetomium for biological control of Fusarium wilt of tomato in PR China." Proceedings of the 5th International Conference on Plant Protection in the Tropics. 1999.
  17. ↑ Inglis, G D; Kawchuk, L M (1 January 2002). "Comparative degradation of oomycete, ascomycete, and basidiomycete cell walls by mycoparasitic and biocontrol fungi". Canadian Journal of Microbiology. 48 (1): 60–70. doi:10.1139/w01-130. ISSN   0008-4166. PMID   11888164.
  18. ↑ Chiu, S. C.; Tzean, S. S. (1 February 1995). "Glucanolytic enzyme production by Schizophyllum commune Fr. during mycoparasitism". Physiological and Molecular Plant Pathology. 46 (2): 83–94. doi:10.1006/pmpp.1995.1007.
  19. ↑ Bruce, A.; Srinivasan, U.; Staines, H. J.; Highley, T. L. (1 January 1995). "Chitinase and laminarinase production in liquid culture by Trichoderma spp. and their role in biocontrol of wood decay fungi". International Biodeterioration & Biodegradation. 35 (4): 337–353. doi:10.1016/0964-8305(95)00047-3.
  20. ↑ Dean, J.F.D; Gamble, H.R; Anderson, J.D (1989). "The ethylene biosynthesis-inducing Xylanase:Its induction in Trichoderma viride and Certain Plant Pathogens". Phytopathology.
  21. ↑ Zeilinger, Susanne; Galhaup, Christiane; Payer, Kathrin; Woo, Sheridan L.; Mach, Robert L.; Fekete, Csaba; Lorito, Matteo; Kubicek, Christian P. (1 March 1999). "Chitinase Gene Expression during Mycoparasitic Interaction ofTrichoderma harzianumwith Its Host". Fungal Genetics and Biology. 26 (2): 131–140. doi:10.1006/fgbi.1998.1111. PMID   10328983.
  22. ↑ Kanokmedhakul, Somdej; Kanokmedhakul, Kwanjai; Nasomjai, Pitak; Louangsysouphanh, Sysavad; Soytong, Kasem; Isobe, Minoru; Kongsaeree, Palangpon; Prabpai, Samran; Suksamrarn, Apichart (1 June 2006). "Antifungal Azaphilones from the Fungus Chaetomium cupreum CC3003". Journal of Natural Products. 69 (6): 891–895. doi:10.1021/np060051v. ISSN   0163-3864. PMID   16792406.
  23. ↑ Fleiβner, André; Sopalla, Claudia; Weltring, Klaus-Michael (1 February 2002). "An ATP-binding Cassette Multidrug-Resistance Transporter Is Necessary for Tolerance of Gibberella pulicaris to Phytoalexins and Virulence on Potato Tubers". Molecular Plant-Microbe Interactions. 15 (2): 102–108. doi: 10.1094/MPMI.2002.15.2.102 . ISSN   0894-0282. PMID   11876422.
  24. ↑ Kanokmedhakul, Somdej; Kanokmedhakul, Kwanjai; Nasomjai, Pitak; Louangsysouphanh, Sysavad; Soytong, Kasem; Isobe, Minoru; Kongsaeree, Palangpon; Prabpai, Samran; Suksamrarn, Apichart (1 June 2006). "Antifungal Azaphilones from the Fungus Chaetomium cupreum CC3003". Journal of Natural Products. 69 (6): 891–895. doi:10.1021/np060051v. ISSN   0163-3864. PMID   16792406.
  25. 1 2 Hung, Phung Manh; Wattanachai, Pongnak; Kasem, Soytong; Poaim, Supatta (2015). "Biological Control of Phytophthora palmivora Causing Root Rot of Pomelo Using Chaetomium spp". Mycobiology. 43 (1): 63–70. doi:10.5941/myco.2015.43.1.63. PMC   4397382 . PMID   25892917.
  26. ↑ Corrochano, Luis M. (2007). "Fungal photoreceptors: sensory molecules for fungal development and behaviour". Photochemical & Photobiological Sciences. 6 (7): 725–36. doi:10.1039/b702155k. hdl: 11441/40641 . PMID   17609765. S2CID   12309173.
  27. ↑ Johns, M. R; Chong, R; Maddox, I. S (1982). "Hydrolysis of some natural and synthetic bile acid conjugates by Cercospora melonis". Can J Microbiol. 28 (5): 457–61. doi:10.1139/m82-069. PMID   7201881.
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