Copromyxa protea

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Copromyxa protea
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Eukaryota
Phylum: Amoebozoa
Class: Tubulinea
Order: Euamoebida
Family: Hartmannellidae
Genus: Copromyxa
Species:
C. protea
Binomial name
Copromyxa protea
(Fayod) Zopf

Copromyxa protea is a cellular slime mold belonging to the supergroup Amoebozoa. The taxonomical history of C. protea starts as Guttulina protea and ultimately ends with becoming its own genus of protists. Its morphological features like tubular mitochondrial cristae help pinpoint its placement in its supergroup, as well as its pseudopodia. The life cycle of this cellular slime mold is outlined and expanded upon, delving into specific characteristics of each component in the stages. The specific environments where C. protea was gathered for experimental purposes is also detailed.

Contents

Taxonomy and taxonomic history

Based on physical appearance alone, C. protea was placed in Amoebozoa due to its tubular mitochondrial cristae and broad lobose pseudopodia. [1]

More recently, sequencing of small nuclear encoded subunit ribosomal RNA genes led the genus of Copromyxa to be placed in the Amoebozoa supergroup. Within this supergroup, it was found to be in the group Tubulinea. This came across as unexpected due to the fact that this group was recorded to have no slime molds before this taxonomic distinction. [2] Cellular slime molds were once organized into two major categories—Dictyostelia and Acrasea. C. protea fell into the latter of the two. However, further research has placed Acrasids into the supergroup Excavata, leaving C. protea to be excluded from this grouping. [3]

Before becoming Copromyxa protea, Victor Fayod declared the name Guttulina protea for this amoeba. It was shortly changed thereafter due to Guttulina fruiting bodies having multiple cell types, while Copromyxa just consists of one. This change was founded and implemented by Friedrich Wilhelm Zopf in 1885. [4]

It now exists within a four-species clade of Copromyxa/Hartmannella. However, Hartmannella has since been renamed to the genus Copromyxa due to the similarities of the two. [5]

Morphology

The features of C. protea that place it in Amoebozoa and Tubulinea are its tubular mitochondrial christae, monopodial amoeba measuring around 23 µm, and central nucleus. [6]

The appearance of the sorocarp, its mature fruiting body, is white to pale yellow, reaching a height range of 300–800 µm. The sorocysts along the sorocarps can be arranged in a column or in more complex forms. [6] The sorocyst is an encysted cell of a single type found on the sorocarp. [2] The sorocysts themselves can be different shapes including oblong, ovoid, and angular, and they produce only one monopodial amoeba. They are smooth-walled hyaline and uninucleated as well, averaging 8–13 µm in size. [6]

Upon germination, a sorocyst becomes an amoeba. The amoeba is limax-shaped and uninucleate. Their single pseudopodia consists of a clear hyaline cap, allowing the amoeba to move fast. The dimensions of the amoeba average to be 23 µm in length and 7 µm in width. Within the amoeba, the nucleus size is measured at 4 µm averagely with a central nucleolus. [6]

In culture, an amoeba germinated from a sorocyst develops into a sphaerocyst after a short period of growth. The appearance of its sphaerocyst is distinguishable: double-walled, brownish-yellow, and generally rounder than sorocysts and microcysts measuring 12–13 µm in diameter. These thick-walled sphareocysts occur after two amoeba undergo plasmogamy followed by karyogamy. [6]

The microcyst is nearly identical to the morphology of the sorocyst. The difference lies in the stage of the life cycle each is produced, and microcysts may tend to be more irregular in shape like a puzzle piece. Rather than encyst on the sorocarp like a sorocyst, microcysts are a result of an amoeba encysting on a substrate. Once germinated, the amoeba leaves behind a wall. [6]

Behavior and life cycle

Copromyxa protea is the first slime mold found to be in the class Tubulinea of the supergroup Amoebozoa. More specifically, it is a cellular slime mold meaning an amoeboid protist that creates fruiting bodies. [2]

As mentioned previously, the sorocarp is a mature fruiting body containing a sorocyst. Upon germination, the sorocyst develops into the monopodial amoeba. Microcysts are also able to germinate into amoeba. [6]

The amoeba have the potential to encyst on the sorocarp to become founder cells. Once the development of a founder cell occurs, aggregation of amoeba ensues. How exactly the founder cell lures the amoeba to itself to aggregate is not yet known. The amoeba pile on top of the founder cell and encyst themselves, repeating until the fruiting body is elongated. A sorocarp can take the form of uniaxial or branched. [6]

A sexual cycle also seems to be apparent. Uninucleate trophic amoeba proceed with plasmogamy and karyogamy which leads to the formation of a sphaerocyst. [6] However, confirmation of secondary meiosis is yet to be established. [3] Within culture, the sphaerocyst stage of the life cycle seems to lead to a halt. No germination has been observed following the formation of a sphaerocyst. This is only within lab settings, and germination is presumed to occur naturally in the wild. [6]

The life cycle of cellular slime molds is ultimately separated into two main divisions. The trophic stage consists of the singular amoeba described above. Once the amoeba starts aggregating and establishing found cells, then the fruiting stage is kicked off. The fruiting bodies bear sorocysts to restart the life cycle. [6]

Ecology

Copromyxa protea can be found on the excrement of many bovine and equine creatures. Within the United States, it is popularly found on the fecal matter of cattle and horses. C. protea can use Escherichia coli(E. coli) as a nutrient source. [6]

Related Research Articles

<span class="mw-page-title-main">Slime mold</span> Spore-forming organisms

Slime mold or slime mould is an informal name given to a polyphyletic assemblage of unrelated eukaryotic organisms in the Stramenopiles, Rhizaria, Discoba, Amoebozoa and Holomycota clades. Most are microscopic; those in the Myxogastria form larger plasmodial slime molds visible to the naked eye. The slime mold life cycle includes a free-living single-celled stage and the formation of spores. Spores are often produced in macroscopic multicellular or multinucleate fruiting bodies that may be formed through aggregation or fusion; aggregation is driven by chemical signals called acrasins. Slime molds contribute to the decomposition of dead vegetation; some are parasitic.

<span class="mw-page-title-main">Dictyostelid</span> Group of slime moulds

The dictyostelids or cellular slime molds are a group of slime molds or social amoebae.

<span class="mw-page-title-main">Mycetozoa</span> Infraphylum of protists

Mycetozoa is a polyphyletic grouping of slime molds. It was originally thought to be a monophyletic clade, but recently it was discovered that protostelia are a polyphyletic group within Conosa.

<span class="mw-page-title-main">Amoebozoa</span> Phylum of protozoans

Amoebozoa is a major taxonomic group containing about 2,400 described species of amoeboid protists, often possessing blunt, fingerlike, lobose pseudopods and tubular mitochondrial cristae. In traditional classification schemes, Amoebozoa is usually ranked as a phylum within either the kingdom Protista or the kingdom Protozoa. In the classification favored by the International Society of Protistologists, it is retained as an unranked "supergroup" within Eukaryota. Molecular genetic analysis supports Amoebozoa as a monophyletic clade. Modern studies of eukaryotic phylogenetic trees identify it as the sister group to Opisthokonta, another major clade which contains both fungi and animals as well as several other clades comprising some 300 species of unicellular eukaryotes. Amoebozoa and Opisthokonta are sometimes grouped together in a high-level taxon, variously named Unikonta, Amorphea or Opimoda.

<span class="mw-page-title-main">Rhizaria</span> Infrakingdom of protists

The Rhizaria are a diverse and species-rich supergroup of mostly unicellular eukaryotes. Except for the Chlorarachniophytes and three species in the genus Paulinella in the phylum Cercozoa, they are all non-photosynthethic, but many foraminifera and radiolaria have a symbiotic relationship with unicellular algae. A multicellular form, Guttulinopsis vulgaris, a cellular slime mold, has been described. This group was used by Cavalier-Smith in 2002, although the term "Rhizaria" had been long used for clades within the currently recognized taxon.

<span class="mw-page-title-main">Lobosa</span> Phylum of protozoans

Lobosa is a taxonomic group of amoebae in the phylum Amoebozoa. Most lobosans possess broad, bluntly rounded pseudopods, although one genus in the group, the recently discovered Sapocribrum, has slender and threadlike (filose) pseudopodia. In current classification schemes, Lobosa is a subphylum, composed mainly of amoebae that have lobose pseudopods but lack cilia or flagella.

<i>Chaos</i> (genus) Genus of microscopic organisms

Chaos is a genus of single-celled amoeboid organisms in the family Amoebidae. The largest and most-known species, the so-called "giant amoeba", can reach lengths up to 5 mm, although most specimens fall between 1 and 3 mm.

<span class="mw-page-title-main">Amoebidae</span> Family of protozoans

The Amoebidae are a family of Amoebozoa, including naked amoebae that produce multiple pseudopodia of indeterminate length. These are roughly cylindrical with granular endoplasm and no subpseudopodia, as found in other members of the class Tubulinea. During locomotion one pseudopod typically becomes dominant and the others are retracted as the body flows into it. In some cases the cell moves by "walking", with relatively permanent pseudopodia serving as limbs.

<span class="mw-page-title-main">Tubulinea</span> Class of protozoans

The Tubulinea are a major grouping of Amoebozoa, including most of the more familiar amoebae genera like Amoeba, Arcella, Difflugia and Hartmannella.

<span class="mw-page-title-main">Acrasidae</span> Family of slime moulds

The family Acrasidae is a family of slime molds which belongs to the excavate group Percolozoa. The name element acrasio- comes from the Greek akrasia, meaning "acting against one's judgement". This group consists of cellular slime molds.

<span class="mw-page-title-main">Eumycetozoa</span> Taxonomic group of slime molds

Eumycetozoa, or true slime molds, is a diverse group of protists that behave as slime molds and develop fruiting bodies, either as sorocarps or as sporocarps. It is a monophyletic group or clade within the phylum Amoebozoa that contains the myxogastrids, dictyostelids and protosporangiids.

<i>Dictyostelium discoideum</i> Species of slime mould

Dictyostelium discoideum is a species of soil-dwelling amoeba belonging to the phylum Amoebozoa, infraphylum Mycetozoa. Commonly referred to as slime mold, D. discoideum is a eukaryote that transitions from a collection of unicellular amoebae into a multicellular slug and then into a fruiting body within its lifetime. Its unique asexual life cycle consists of four stages: vegetative, aggregation, migration, and culmination. The life cycle of D. discoideum is relatively short, which allows for timely viewing of all stages. The cells involved in the life cycle undergo movement, chemical signaling, and development, which are applicable to human cancer research. The simplicity of its life cycle makes D. discoideum a valuable model organism to study genetic, cellular, and biochemical processes in other organisms.

<span class="mw-page-title-main">Protosteliales</span> Group of slime moulds

Protosteliomycetes/Protosteliales (ICBN) or Protostelea/Protostelia/Protosteliida (ICZN) is a grouping of slime molds from the phylum Mycetozoa. The name can vary depending upon the taxon used. Other names include Protostelea, Protostelia, and Protostelida. When not implying a specific level of classification, the term protostelid or protosteloid amoeba is sometimes used.

Copromyxa is a genus of Amoebozoa in the Lobosa lineage of the eukaryotic supergroup Amoebozoa. It currently includes 2 species, the sorocarpic amoeba Copromyxa protea and the non-sorocarpic amoeba Copromyxa (=Hartmannella) cantabrigiensis. The named species Copromyxa arborescens, is a synonym of C. protea.

Fonticula is a genus of cellular slime mold which forms a fruiting body in a volcano shape. As long ago as 1979 it has been known to not have a close relationship with either the Dictyosteliida or the Acrasidae, the two well-established groups of cellular slime molds. In 1979, Fonticula was made a new genus of its own due to the unique characteristics of its fruiting body, with only one species: Fonticula alba.

<span class="mw-page-title-main">Amoeba</span> Cellular body type

An amoeba, often called an amoeboid, is a type of cell or unicellular organism with the ability to alter its shape, primarily by extending and retracting pseudopods. Amoebae do not form a single taxonomic group; instead, they are found in every major lineage of eukaryotic organisms. Amoeboid cells occur not only among the protozoa, but also in fungi, algae, and animals.

<i>Luapeleamoeba hula</i> Species of acanthamoebid amoeba

Luapeleamoeba hula is a species of acanthamoebid amoeba described in 2016, capable of producing protosteloid fruiting bodies that consist of a stalk with one spore. It was obtained from dead māmaki leaves from the Manuka Natural Area Reserve in Hawai'i. It has also been found in the Democratic Republic of Congo.

<i>Syssomonas</i> Genus of protists

Syssomonas is a monotypic genus of unicellular flagellated protists containing the species Syssomonas multiformis. It is a member of Pluriformea inside the lineage of Holozoa, a clade containing animals and their closest protistan relatives. It lives in freshwater habitats. It has a complex life cycle that includes unicellular amoeboid and flagellated phases, as well as multicellular aggregates, depending on the growth medium and nutritional state.

<span class="mw-page-title-main">Amoeboflagellate</span> Cellular body type

An amoeboflagellate is any eukaryotic organism capable of behaving as an amoeba and as a flagellate at some point during their life cycle. Amoeboflagellates present both pseudopodia and at least one flagellum, often simultaneously.

<i>Protosteliopsis fimicola</i> Species of amoeba

Protosteliopsis fimicola is an amoeba that forms a fruiting body that consists of a single spore with a non-cellular stalk. This species was thought to be closely related to the species P. mycophya, but it was found that it has a significant difference from this species because of having an irregular stalk and non-deciduous spores. Later it was found that Protosteliopsis is a part of group 6 in the genus Vannella based on 18s rRNA molecular phylogenetics.

References

  1. Dykstra, Michael J. (1977). "The Possible Phylogenetic Significance of Mitochondrial Configurations in the Acrasid Cellular Slime Molds with Reference to Members of the Eumycetozoa and Fungi". Mycologia. 69 (3): 579–591. doi:10.1080/00275514.1977.12020096. PMID   559936.
  2. 1 2 3 Brown, Matthew W. (May 2010). Placing the forgotten slime molds (Sappinia, Copromyxa, Fonticula, Acrasis, and Pocheina), using molecular phylogenetics (PhD thesis). ProQuest   305185206.
  3. 1 2 Lahr, Daniel J. G.; Parfrey, Laura Wegener; Mitchell, Edward A. D.; Katz, Laura A.; Lara, Enrique (2011-07-22). "The chastity of amoebae: re-evaluating evidence for sex in amoeboid organisms". Proceedings of the Royal Society B: Biological Sciences. 278 (1715): 2081–2090. doi: 10.1098/rspb.2011.0289 . PMC   3107637 . PMID   21429931.
  4. Nesom, Margaret; Olive, L. S. (1972). "Copromyxa Arborescens, A New Cellular Slime Mold". Mycologia. 64 (6): 1359–1362. doi:10.1080/00275514.1972.12019392.
  5. Kostka, Martin; Lares-Jiménez, Luis Fernando; Dyková, Iva (May 2015). Phylogenetic placement of Hartmannella cantabrigiensis to Copromyxa inferred from multigene analysis. XVI International Meeting on the Biology and Pathogenicity of Free-living AmoebaeAt: Alghero, Italy.
  6. 1 2 3 4 5 6 7 8 9 10 11 12 Brown, Matthew W.; Silberman, Jeffrey D.; Spiegel, Frederick W. (2011-04-01). "'Slime Molds' among the Tubulinea (Amoebozoa): Molecular Systematics and Taxonomy of Copromyxa". Protist. 162 (2): 277–287. doi:10.1016/j.protis.2010.09.003. PMID   21112814.

Further reading