Didymella bryoniae | |
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Symptoms of D. bryoniae on watermelon | |
Scientific classification | |
Domain: | Eukaryota |
Kingdom: | Fungi |
Division: | Ascomycota |
Class: | Dothideomycetes |
Order: | Pleosporales |
Family: | Didymellaceae |
Genus: | Didymella |
Species: | D. bryoniae |
Binomial name | |
Didymella bryoniae (Fuckel) Rehm, (1881) | |
Synonyms | |
Ascochyta citrullina(Chester) C.O. Sm., (1905) Contents |
Didymella bryoniae, syn. Mycosphaerella melonis, is an ascomycete fungal plant pathogen that causes gummy stem blight on the family Cucurbitaceae (the family of gourds and melons), which includes cantaloupe, cucumber, muskmelon and watermelon plants. [1] [2] [3] [4] The anamorph/asexual stage for this fungus is called Phoma cucurbitacearum. [2] When this pathogen infects the fruit of cucurbits it is called black rot. [2]
The first symptoms appear as grayish green, circular spots between the veins of the leaf lobes. [1] With age these spots darken to brown and black. [1] [2] Lesions begin to develop on vines at the vine nodes and then elongate into water-soaked streaks, and these streaks are pale brown at first but turn gray with time. [1] The petioles and stems eventually become necrotic and often shrivel. Eventually all infected vines will become necrotic and occasionally the plant dies due to wilting and defoliation. [1] [5] Another common sign following the stem lesions is a red to amber colored ooze. [6] Some regions report the presence of small pseudothecia as black specks inside the cankers. [7]
Gummy stem blight can be confused with anthracnose, which is caused by a fungal plant pathogen called Colletotrichum lagenarium . [1] To distinguish between anthracnose and gummy stem blight, gummy stem blight leaf lesions are darker, target-like and less deteriorated than anthracnose lesions. [1] Newly infected plants will begin to show symptoms within 7–12 days.
In vitro, the fungal growth on an agar plate looks rough and undulated. [5] When grown in vitro on agar, the fungus produces a white to olive-colored mycelium. In latter periods of growth, the mycelium is an olive to dark green or black color. [5]
Didymella bryoniae survives on deceased vines, crop debris and on seeds in between seasons and D. bryoniae can survive for 5 months on the soil surface in winter. [2] [4] The fungus develops best under moist conditions, and cotyledons and young watermelon/melon leaves are especially susceptible to the fungus. [2] D. bryoniae produces ascospores (meiotic spores) in perithecia and conidia (mitotic spores) in pycnidia and both of these spores are dispersed by rain/rain-splash and UV light is needed in order for the fungus to sporulate. [3] Ideal ascospore dispersal occurs after nightly rainfall and dew periods. [2] In order to infect, ascospores must land on leaves that have free-standing water on them. [2] Next the ascospores penetrate through the leaf cuticle. [2] Stems may be infected by D. bryoniae ascospores through stem wounds or by the extension of leaf lesions. [2] Fruits are penetrated through wounds and pollination flower scars. [2] Conidia are produced on the lesion sites of leaves and stems. Certain Cucurbita species are resistant to D. bryoniae but become vulnerable once they mature. [2]
Didymella bryoniae is common in the Southern U.S. and other subtropical or tropical locations. [2] Most infections occur during rainy/wet seasons, in which the humid is greater than 90% and the temperature is roughly 20–24 °C. [8] Humidity seems to be a larger factor than temperature when it comes to infection success. [2] D. bryoniae can also be found in temperate regions, especially where winter squash and pumpkins are grown. [2] This pathogen is also common in greenhouses where cucumbers are grown. [2]
D. bryoniae can be spread by the transfer of conidia through a variety of fashions. The most common forms of transfer for these conidia are through the air and water splashing. The fungus is capable of surviving in dead plant tissue giving it the ability to infect the following crop planting. [9] The pathogen requires an entry site on the plant in order to infect so areas that also experience issues with pests are at higher risk.
In vitro, D. bryoniae does not form pycnidia without UV-light but if cultured in the presence of UV light and darkness, conidia/pycnidiospores produce mycelium rapidly. [10]
The standard management practice for D. bryoniae is to use pesticide treated/pathogen-free seeds and to rotate crops on a 2-year cycle to reduce inoculum prevalence. [2] There are no commercially acceptable resistant cucumbers, melons or watermelons available yet on the market, but some plant breeders have identified D. bryoniae resistant genes, such as the gene db in watermelon. [2] [11] Regular benzimidazole fungicide applications can control this pathogen, but certain D. bryoniae isolates have been found to be resistant to benzimidazole fungicides in greenhouse settings and in the field. [2]
Along with fungicides, it is important to have proper ventilation and irrigation practices in greenhouse settings. [2] Proper irrigation and ventilation can be utilized to prevent water buildup on leaves. [2] Also to prevent disease onset in greenhouse settings, use UV-absorbing vinyl film, to prevent fungal sporulation. [12]
Currently cultural practices and fungicides work well in greenhouses and in the field only if D. bryoniae is diagnosed in the early stages of disease development. [4] Molecular tools such as polymerase chain reaction (PCR), PCR-enzyme-linked immunosorbent assay and magnetic-capture hybridization multiplex real-time PCR are used to diagnose D. bryoniae in the early stages disease development, although these molecular tools may only be useful for specific isolates of D. bryoniae. [4] [13] [14]
The United States consumed 15.69 pounds of watermelon per capita in the year 2018 after a rise in both total imports and locally produced watermelons. [15] Florida and Georgia characterized 35 isolates of Didymella and phoma spp. Associated with symptoms of gummy stem blight on watermelon. These two states produced 42% of the United States total watermelon value in 2013, and a combined 20,000 hectares in total farm area. Florida alone produced 907 million pounds of watermelon in 2019 [16] meaning that this pathogen could have a direct effect on at least 25% of domestic watermelon crop in the United States.
Grape black rot is a fungal disease caused by an ascomycetous fungus, Guignardia bidwellii, that attacks grape vines during hot and humid weather. “Grape black rot originated in eastern North America, but now occurs in portions of Europe, South America, and Asia. It can cause complete crop loss in warm, humid climates, but is virtually unknown in regions with arid summers.” The name comes from the black fringe that borders growing brown patches on the leaves. The disease also attacks other parts of the plant, “all green parts of the vine: the shoots, leaf and fruit stems, tendrils, and fruit. The most damaging effect is to the fruit”.
Diplocarpon rosae is a fungus that creates the rose black spot disease. Because it was observed by people of various countries around the same time, the nomenclature for the fungus varied with about 25 different names. The asexual stage is now known to be Marssonina rosae, while the sexual and most common stage is known as Diplocarpon rosae.
Leptosphaeria maculans is a fungal pathogen of the phylum Ascomycota that is the causal agent of blackleg disease on Brassica crops. Its genome has been sequenced, and L. maculans is a well-studied model phytopathogenic fungus. Symptoms of blackleg generally include basal stem cankers, small grey lesions on leaves, and root rot. The major yield loss is due to stem canker. The fungus is dispersed by the wind as ascospores or rain splash in the case of the conidia. L. maculans grows best in wet conditions and a temperature range of 5–20 degrees Celsius. Rotation of crops, removal of stubble, application of fungicide, and crop resistance are all used to manage blackleg. The fungus is an important pathogen of Brassica napus (canola) crops.
Alternaria alternata is a fungus causing leaf spots, rots, and blights on many plant parts, and other diseases. It is an opportunistic pathogen on over 380 host species of plant.
Pseudocercosporella capsellae is a plant pathogen infecting crucifers. P. capsellae is the causal pathogen of white leaf spot disease, which is an economically significant disease in global agriculture. P. capsellae has a significant affect on crop yields on agricultural products, such as canola seed and rapeseed. Researchers are working hard to find effective methods of controlling this plant pathogen, using cultural control, genetic resistance, and chemical control practices. Due to its rapidly changing genome, P. capsellae is a rapidly emerging plant pathogen that is beginning to spread globally and affect farmers around the world.
Leptosphaeria coniothyrium is a plant pathogen. It can be found around the world.
Ascochyta tarda or Phoma tarda is a fungal plant pathogen that causes dieback and leafspot on coffee and was first observed in Ethiopia in 1954. It poses a potentially serious threat to coffee crops, but climate change may reduce the prevalence of environmental conditions favorable to its spread.
Diaporthe helianthi is a fungal pathogen that causes Phomopsis stem canker of sunflowers. In sunflowers, Phomopsis helianthi is the causative agent behind stem canker. Its primary symptom is the production of large canker lesions on the stems of sunflower plants. These lesions can eventually lead to lodging and plant death. This disease has been shown to be particularly devastating in southern and eastern regions of Europe, although it can also be found in the United States and Australia. While cultural control practices are the primary method of controlling for Stem Canker, there have been a few resistant cultivars developed in regions of Europe where the disease is most severe.
Ascochyta is a genus of ascomycete fungi, containing several species that are pathogenic to plants, particularly cereal crops. The taxonomy of this genus is still incomplete. The genus was first described in 1830 by Marie-Anne Libert, who regarded the spores as minute asci and the cell contents as spherical spores. Numerous revisions to the members of the genus and its description were made for the next several years. Species that are plant pathogenic on cereals include, A. hordei, A. graminea, A. sorghi, A. tritici. Symptoms are usually elliptical spots that are initially chlorotic and later become a necrotic brown. Management includes fungicide applications and sanitation of diseased plant tissue debris.
Phomopsis obscurans is a common fungus found in strawberry plants, which causes the disease of leaf blight. Common symptoms caused by the pathogen begin as small circular reddish-purple spots and enlarge to form V-shaped lesions that follow the vasculature of the plant's leaves. Although the fungus infects leaves early in the growing season when the plants are beginning to develop, leaf blight symptoms are most apparent on older plants towards the end of the growing season. The disease can weaken strawberry plants through the destruction of foliage, which results in reduced yields. In years highly favorable for disease development, leaf blight can ultimately lead to the death of the strawberry plants. A favorable environment for the growth and development of the Phomopsis obscurans pathogen is that of high temperature, high inoculum density, a long period of exposure to moisture, and immature host tissue. In the case of disease management, a conjunction of cultural practices is the most effective way of reducing the infection.
Ascochyta pisi is a fungal plant pathogen that causes ascochyta blight on pea, causing lesions of stems, leaves, and pods. These same symptoms can also be caused by Ascochyta pinodes, and the two fungi are not easily distinguishable.
Phoma macdonaldii is a plant pathogenic fungus that is a major causal force for the disease Phoma Black Stem.
Elsinoë ampelina is a plant pathogen, which is the causal agent of anthracnose on grape.
Calophoma clematidina is a fungal plant pathogen and the most common cause of the disease clematis wilt affecting large-flowered varieties of Clematis. Symptoms of infection include leaf spotting, wilting of leaves, stems or the whole plant and internal blackening of the stem, often at soil level. Infected plants growing in containers may also develop root rot.
Raspberry spur blight is caused by the fungus Didymella applanata. This plant pathogen is more problematic on red raspberries (Rubus idaeus) than on black or purple raspberries. The fungus infects the leaves first and then spreads to the cane. It causes necrotic spots on the cane near the base of the petiole attachment. Raspberry spur blight can cause a significant reduction in yield, fruit blight, premature leaf drop, and weak bud and cane growth. The magnitude of damage is not clearly understood in the United States, however, studies from Scotland suggest damage to the cane itself is limited. The disease has minor economic impacts by reducing leaves in the summer or killing buds. Major economic damage occurs if the disease manages to kill the entire cane. In the United States, this disease is found in Oregon and Washington.
Ascochyta blights occur throughout the world and can be of significant economic importance. Three fungi contribute to the ascochyta blight disease complex of pea. Ascochyta pinodes causes Mycosphaerella blight. Ascochyta pinodella causes Ascochyta foot rot, and Ascochyta pisi causes Ascochyta blight and pod spot. Of the three fungi, Ascochyta pinodes is of the most importance. These diseases are conducive under wet and humid conditions and can cause a yield loss of up to fifty percent if left uncontrolled. The best method to control ascochyta blights of pea is to reduce the amount of primary inoculum through sanitation, crop-rotation, and altering the sowing date. Other methods—chemical control, biological control, and development of resistant varieties—may also be used to effectively control ascochyta diseases.
Phomopsisblight of juniper is a foliar disease discovered in 1917 caused by the fungal pathogen Phomopsis juniperovora. The fungus infects new growth of juniper trees or shrubs, i.e. the seedlings or young shoots of mature trees. Infection begins with the germination of asexual conidia, borne from pycnidia, on susceptible tissue, the mycelia gradually move inwards down the branch, and into the main stem. Management strategies mainly include removing and destroying diseased tissue and limiting the presence of moisture on plants. Junipers become resistant to infection as they mature and the young yellow shoots turn dark green. Preventive strategies include planting only resistant varieties and spraying new growth with fungicide until plants have matured.
Raspberry leaf spot is a plant disease caused by Sphaerulina rubi, an ascomycete fungus. Early symptoms of infection are dark green spots on young leaves. As the disease progresses, these spots turn tan or gray in color. Disease management strategies for raspberry leaf spots include the use of genetically resistant raspberry plant varieties, chemical fungicide sprays, and cultural practices such as pruning and thinning out canes.
Gummy stem blight is a cucurbit-rot disease caused by the fungal plant pathogen Didymella bryoniae. Gummy stem blight can affect a host at any stage of growth in its development and affects all parts of the host including leaves, stems and fruits. Symptoms generally consist of circular dark tan lesions that blight the leaf, water soaked leaves, stem cankers, and gummy brown ooze that exudes from cankers, giving it the name gummy stem blight. Gummy stem blight reduces yields of edible cucurbits by devastating the vines and leaves and rotting the fruits. There are various methods to control gummy stem blight, including use of treated seed, crop rotation, using preventative fungicides, eradication of diseased material, and deep plowing previous debris.