State Road 100 is a 153-mile-long (246 km) east–west highway serving northeast Florida. Its western terminus is at the Georgia-Florida border four miles (6.4 km) north of Avoca, Florida ; its eastern terminus is an intersection with Shore Scenic Highway in Flagler Beach. The portion west of Lake City is only signed as portions of US 41 and US 129, both of which run north–south.
State Road 200 is a major diagonal road in central and northeast Florida. Its southern terminus is at US 41 in Hernando. Its eastern terminus is at SR A1A in Fernandina Beach, at the corner of Atlantic Avenue and Fletcher Avenue.
State Road 20 is a 358.154-mile-long (576.393 km) east-to-west route across northern Florida and the Florida Panhandle in the United States.
State Road 44 is an east–west state highway in the U.S. state of Florida. It runs from Crystal River on the Gulf of Mexico east to New Smyrna Beach on the Atlantic Ocean, passing through Inverness, Wildwood, Leesburg and DeLand.
State Road 24 is an east–west state highway that runs between Cedar Key on the Gulf of Mexico and Waldo, Florida, at US 301. State Road 24 runs along a former branch of the Seaboard Air Line Railroad. This branch was originally owned by the Florida Railway and Navigation Company, and used to lead to ferries to both Pensacola, Florida and New Orleans, Louisiana. It also passes through Rosewood, site of the infamous 1923 massacre.
State Road 26 is a 62.172-mile-long (100.056 km) east–west route across North Central Florida.
State Road 19 is a Florida State Road in Putnam, Marion, and Lake counties. It runs from Groveland to Palatka through Ocala National Forest. Along with SR 33, SR 19 provides a mostly rural north-south corridor through central Florida from Lakeland to Palatka.
U.S. Highway 27 (US 27) in Florida is a north–south United States Numbered Highway. It runs 496.352 miles (798.801 km) from the Miami metropolitan area northwest to the Tallahassee metropolitan area. Throughout the state, US 27 has been designated the Claude Pepper Memorial Highway by the Florida Legislature. It was named after long-time Florida statesperson Claude Pepper, who served in both the U.S. Senate and House of Representatives. The stretch running from Miami to South Bay was originally designated the Thomas E. Will Memorial Highway by the Florida Legislature in 1937 when that portion was known as State Road 26 (SR 26). Thomas E. Will, the founder of Okeelanta, had worked for almost 20 years to get the state to build a road from Miami to the area south of Lake Okeechobee. For most of its length in the state, US 27 is a divided highway.
U.S. Highway 41 (US 41) in the state of Florida is a north–south United States Highway. It runs 479 miles (771 km) from Miami in South Florida northwest to the Georgia state line north of the Lake City area. Within the state, US 41 is paralleled by Interstate 75 (I-75) all the way from Miami to Georgia, and I-75 has largely supplanted US 41 as a major highway.
U.S. Route 98 is a major east-west thoroughfare through the U.S. state of Florida. Spanning 670.959 miles (1,079.804 km), it connects Pensacola and the Alabama/Florida state line to the west with Palm Beach and the Atlantic coast in the east. It is the longest US road in Florida, as well as the longest US road in any state east of the Mississippi River.
U.S. Route 301 in Florida runs from the Sarasota-Bradenton-Venice, Florida Metropolitan Statistical Area northeast to the Greater Jacksonville Metropolitan Area. The road is a spur of U.S. Route 1, which it intersects in Callahan.
U.S. Route 441 (US 441) in Florida is a north–south United States Highway. It runs 433 miles (697 km) from Miami in South Florida northwest to the Georgia state line, with the overall route continuing to Tennessee in the Rocky Top area.
State Road 121 is a major state highway that runs north and south in northern Florida. The road is part of a long multi-state route that also goes through Georgia and South Carolina.
State Road 18 is a short state road in the U.S. state of Florida. It begins in Worthington Springs in southern Union County and travels eastward for 6.60 miles (10.62 km) into Bradford County until it becomes County Road 18 at Brooker. It also has county road extensions, one of which is between Fort White and Worthington Springs, and the other between Brooker and SR 100 north of Theressa.
State Road 35 is a north–south state highway in the western counties of the U.S. state of Florida.
State Road 329 (SR 329) was a short state highway traveling south and north through Gainesville, Florida. It comprised Main Street through Gainesville. The full length of the road extended from SR 331 to County Road 232 (CR 232). In the 1970s, the northern portion of the road, most of North Main Street, became County Road 329 (CR 329) while South Main Street was relinquished by the Florida Department of Transportation (FDOT) to the city. A small portion of the former state road in Gainesville is still maintained by the state as a portion of SR 20.
State Road 464 is a short state and county road in Marion County, Florida. The road is primarily in southern Ocala, but extends outside of the city limits.
State Road 235 is a south–north state highway in Alachua County, Florida. It runs from U.S. Route 441 in Alachua to Brooker, and has county-maintained segments in Alachua and Bradford Counties, which were once part of SR 235.
State Road 276 is a state road in the panhandle of Florida. It runs west to east from former State Road 167 into U.S. Route 90 in and around Marianna entirely within Jackson County, although a bi-county extension exists between State Road 277 southwest of Chipley in Washington County that runs south then east into CR 167 which is signed as County Road 276 (CR 276). Most of the road is a two-lane undivided highway, with exceptions in the vicinity of Interstate 10, and if you count the bi-county portion, the overlapped segment with U.S. 231 where it changes from a four-lane undivided highway to a four-lane divided highway between Alford and Steele City.
A total of at least twelve special routes of U.S. Route 441 have existed, and at least three have been deleted. These special routes include alternate routes, business loops, truck routes, and bypass routes which connect to U.S. Route 441 in the US states of Florida, Georgia, North Carolina, and Tennessee.
I-195 / US 1 in Miami 
SR 60 in Lake Wales 
SR 44 in Leesburg 
SR 35 in Belleview 
US 90 in Lake City 
US 41 / SR 7 towards Lake Park, GA 
