Geobacillus stearothermophilus | |
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Scientific classification | |
Domain: | Bacteria |
Phylum: | Bacillota |
Class: | Bacilli |
Order: | Bacillales |
Family: | Bacillaceae |
Genus: | Geobacillus |
Species: | G. stearothermophilus |
Binomial name | |
Geobacillus stearothermophilus (Donk 1920) Nazina et al. 2001 | |
Geobacillus stearothermophilus (previously Bacillus stearothermophilus) [1] [2] is a rod-shaped, Gram-positive bacterium and a member of the phylum Bacillota. The bacterium is a thermophile and is widely distributed in soil, hot springs, ocean sediment, and is a cause of spoilage in food products. It will grow within a temperature range of 30–75 °C. Some strains are capable of oxidizing carbon monoxide aerobically. It is commonly used as a challenge organism for sterilization validation studies and periodic check of sterilization cycles. The biological indicator contains spores of the organism on filter paper inside a vial. After sterilizing, the cap is closed, an ampule of growth medium inside of the vial is crushed and the whole vial is incubated. A color and/or turbidity change indicates the results of the sterilization process; no change indicates that the sterilization conditions were achieved, otherwise the growth of the spores indicates that the sterilization process has not been met. Fluorescent-tagged strains, known as rapid-read BIs, [3] are becoming more common to verify sterilization, since the visible fluorescence appears in about one-tenth the time needed for pH-indicator color change and an inexpensive light sensor can detect the growing colonies.
It was first described in 1920 as Bacillus stearothermophilus, [4] but, together with Bacillus thermoglucosidasius , it was reclassified as a member of the genus Geobacillus in 2001. [5]
DNA polymerase I | |||||||
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Identifiers | |||||||
Organism | |||||||
Symbol | PolA | ||||||
PDB | 2XY5 | ||||||
UniProt | E1C9K5 | ||||||
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Thermostable Group II Intron Reverse Transcriptase GsI-IIC | |||||||
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Identifiers | |||||||
Organism | |||||||
Symbol | TRT | ||||||
PDB | 6AR1 | ||||||
UniProt | E2GM63 | ||||||
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Recently, a DNA polymerase derived from these bacteria, Bst polymerase, has become important in molecular biology applications.
Bst polymerase has a helicase-like activity, making it able to unwind DNA strands. Its optimum functional temperature is between 60 and 65 °C and it is denatured at temperatures above 70 °C. These features make it useful in loop-mediated isothermal amplification (LAMP). [6] LAMP is similar to the polymerase chain reaction (PCR) but does not require the high temperature (96 °C) step required to denature DNA.
In 2013, a thermostable group II intron reverse transcriptase (TGIRT), GsI-IIC-MRF, from G. stearothermophilus was found to retain activity up to 70 °C and to exhibit high processivity and a low error rate. [7] These properties make this enzyme useful for reverse transcribing long and/or highly structured RNA molecules. A method for determining RNA secondary structure, DMS-MaPseq, uses this enzyme because it converts normal RNA to DNA accurately but introduces mutations at unpaired bases that have been methylated by dimethyl sulfate, and the mutations can be identified via sequencing. [8]
Thermus is a genus of thermophilic bacteria. It is one of several bacteria belonging to the Deinococcota phylum. According to comparative analysis of 16S rRNA, this is one the most ancient group of bacteria Thermus species can be distinguished from other genera in the family Thermaceae as well as all other bacteria by the presence of eight conserved signature indels found in proteins such as adenylate kinase and replicative DNA helicase as well as 14 conserved signature proteins that are exclusively shared by members of this genus.
Thermus thermophilus is a Gram-negative bacterium used in a range of biotechnological applications, including as a model organism for genetic manipulation, structural genomics, and systems biology. The bacterium is extremely thermophilic, with an optimal growth temperature of about 65 °C (149 °F). Thermus thermophilus was originally isolated from a thermal vent within a hot spring in Izu, Japan by Tairo Oshima and Kazutomo Imahori. The organism has also been found to be important in the degradation of organic materials in the thermogenic phase of composting. T. thermophilus is classified into several strains, of which HB8 and HB27 are the most commonly used in laboratory environments. Genome analyses of these strains were independently completed in 2004. Thermus also displays the highest frequencies of natural transformation known to date.
In materials science and molecular biology, thermostability is the ability of a substance to resist irreversible change in its chemical or physical structure, often by resisting decomposition or polymerization, at a high relative temperature.
In taxonomy, Thermococcus is a genus of thermophilic Archaea in the family the Thermococcaceae.
In taxonomy, Staphylothermus is a genus of the Desulfurococcaceae.
Alicyclobacillus is a genus of Gram-variable, rod-shaped, spore-forming bacteria. The bacteria are able to grow in acidic conditions, while the spores are able to survive typical pasteurization procedures.
Thermoanaerobacter is a genus in the phylum Bacillota (Bacteria). Members of this genus are thermophilic and anaerobic, several of them were previously described as Clostridium species and members of the now obsolete genera Acetogenium and Thermobacteroides
Geobacillus thermoglucosidasius is a thermophilic gram-positive bacterium, and a member of the Bacillota phylum. It was first isolated from soil in Japan in 1983.
Bacillus atrophaeus is a species of black-pigmented bacteria. Its type strain is NRRL NRS-213. B. atrophaeus strains have been used extensively in biomedicine as indicator strains for heat- and chemical-based decontamination regimens. Most of the strains in use are derivatives of a lineage of B. atrophaeus that originated at Camp Detrick in the 1950s, where many modern biocontainment procedures were developed.
Glucan 1,4-alpha-maltohydrolase is an enzyme with systematic name 4-alpha-D-glucan alpha-maltohydrolase. This enzyme catalyses the following chemical reaction
Thermotoga petrophila is a hyperthermophilic, anaerobic, non-spore-forming, rod-shaped, fermentative heterotroph, with type strain RKU-1T. T. petrophila was first discovered and isolated from an oil reservoir off of the coast of Japan and was deemed genetically distinct from its sister clades. Because these organism are found in deep, hot aquatic settings, they have become of great interest for biotechnology due to their enzymes functioning at high temperatures and pressures.
Saccharolobus solfataricus is a species of thermophilic archaeon. It was transferred from the genus Sulfolobus to the new genus Saccharolobus with the description of Saccharolobus caldissimus in 2018.
Geobacillus jurassicus is a thermophilic bacterium first isolated from a high-temperature petroleum reservoir. It is aerobic, gram-positive, rod-shaped, moderately thermophilic, chemoorganotrophic, and endospore-forming, with type species DS1T.
Caldibacillus is a facultative anaerobe genus of bacteria that stains Gram-positive from the family of Bacillaceae. The type species of this genus is Caldibacillus debilis.
Alicyclobacillus acidocaldarius is a species of Gram positive, strictly aerobic, bacterium. The bacteria are acidophilic, thermophilic, and produce endospores. The first identified strains of A. acidocaldarius were from geysers in Yellowstone National Park and fumerole soil in Hawaii Volcano National Park. The species was originally classified as Bacillus acidocaldarius in 1971, but further 16S rRNA studies found that the species belonged in the newly created genus Alicyclobacillus. The species name is derived from the Latin acidum (acid) and caldarius, referring to the acidic and high temperature environments from which it was first isolated. Thomas D. Brock was one of the researchers who first categorized the species; his discovery of Thermus aquaticus allowed for other researchers to discover Taq polymerase and polyermase chain reaction (PCR).
Alkalihalobacillus is a genus of gram-positive or gram-variable rod-shaped bacteria in the family Bacillaceae from the order Bacillales. The type species of this genus is Alkalihalobacillus alcalophilus.
Priestia is a genus of mostly Gram-positive, rod-shaped bacteria in the family Bacillaceae from the order Bacillales. The type species of this genus is Priestia megaterium.
Sutcliffiella is a genus of Gram-Positive rod-shaped bacteria in the family Bacillaceae from the order Bacillales. The type species of this genus is Sutcliffiella cohnii.
Alkalicoccus is a genus of Gram-Positive rod-shaped bacteria in the family Bacillaceae from the order Bacillales. The type species of this genus is Alkalicoccus saliphilus.
Thermostable DNA polymerases are DNA polymerases that originate from thermophiles, usually bacterial or archaeal species, and are therefore thermostable. They are used for the polymerase chain reaction and related methods for the amplification and modification of DNA.