List of restriction enzyme cutting sites: E–F

Last updated January 29, 2025

Legend of nucleobases
Code	Nucleotide represented
A	Adenine (A)
C	Cytosine (C)
G	Guanine (G)
T	Thymine (T)
N	A, C, G or T
M	A or C
R	A or G
W	A or T
Y	C or T
S	C or G
K	G or T
H	A, C or T
B	C, G or T
V	A, C or G
D	A, G or T

This article contains a list of the most studied restriction enzymes whose names start with E to F inclusive. It contains approximately 110 enzymes.

Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature ^[1]^[2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action^[3]^[4].
Source: Organism that naturally produces the enzyme.
Recognition sequence : Sequence of DNA recognized by the enzyme and to which it specifically binds.
Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site^[5]^[6].
Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:

as in this not listed enzyme:

EcoR70I

Whole list navigation

Restriction enzymes

E

Enzyme PDB code Source Recognition sequence Cut Isoschizomers

EacI

Eubacterium acidaminophilum

5' GGATC
3' CCTAG

5' ---GGATCNNNN N--- 3'
3' ---CCTAGNNNNN --- 5'

AclWI, AlwI, BinI,

BpuFI,

BspPI,

BstH9I,

Bst31TI, Bth617I, Ral8I

EaeI Enterobacter aerogenes 5' YGGCCR
3' RCCGGY 5' ---Y GGCCR--- 3'
3' ---RCCGG Y--- 5'

Eae46I Enterobacter aerogenes 46 5' CCGCGG
3' GGCGCC 5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'

EaeAI Enterobacter aerogenes 5' CCCGGG
3' GGGCCC 5' ---C CCGGG--- 3'
3' ---GGGCC C--- 5' AhyI, Cfr9I, EclRI, PaeBI, PspAI, PspALI, XcyI, XmaI, XmaCI

EagI Enterobacter agglomerans 5' CGGCCG
3' GCCGGC 5' ---C GGCCG--- 3'
3' ---GCCGG C--- 5' AaaI, BseX3I, BstZI, EclXI, Eco52I, SenPT16I, XmaIII

EagBI Enterobacter agglomerans CBNU45 5' CGATCG
3' GCTAGC 5' ---CGAT CG--- 3'
3' ---GC TAGC--- 5' Afa16RI, Afa22MI, BspCI, ErhB9I, NblI, Psu161I, RshI, XorII

EagMI Enterobacter agglomerans M3 5' GGWCC
3' CCWGG 5' ---G GWCC--- 3'
3' ---CCWG G--- 5' Bme18I, BsrAI, Csp68KI, Eco47I, FssI, HgiEI, Kzo49I, SmuEI

Eam1104I Enterobacter amnigenus RFL1104 5' CTCTTC
3' GAGAAG 5' ---CTCTTCN NNN--- 3'
3' ---GAGAAGNNNN --- 5'

Eam1105I

Enterobacter amnigenus RFL1105

5' GACN₅GTC
3' CTGN₅CAG

5' ---GACNNN NNGTC--- 3'
3' ---CTGNN NNNCAG--- 5'

AhdI, AspEI,	BmeRI,	BspOVI,
DriI, EclHKI, NruGI

EarI Enterobacter aerogenes 5' CTCTTC
3' GAGAAG 5' ---CTCTTCN NNN--- 3'
3' ---GAGAAGNNNN --- 5'

EcaI Enterobacter cloacae 5' GGTNACC
3' CCANTGG 5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5' AcrII, Bse64I, BseT10I, BstEII, Eco91I, EcoO65I, EcoO128I

EciI Escherichia coli 5' GGCGGA
3' CCGCCT 5' ---GGCGGAN₈NNN --- 3'
3' ---CCGCCTN₈N NN--- 5'

Eci125I Escherichia coli VKM-125 5' GGTNACC
3' CCANTGG 5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5' AcrII, AspAI, BseT10I, BstEII, Eco91I, EcoO65I, EcoO128I

EclI Enterobacter cloacae 5' CAGCTG
3' GTCGAC 5' ---CAG CTG--- 3'
3' ---GTC GAC--- 5'

Ecl136II Enterobacter cloacae RFL136 5' GAGCTC
3' CTCGAG 5' ---GAG CTC--- 3'
3' ---CTC GAG--- 5'

EclHKI

Enterobacter cloacae

5' GACN₅GTC
3' CTGN₅CAG

5' ---GACNNN NNGTC--- 3'
3' ---CTGNN NNNCAG--- 5'

AhdI, AspEI,	BmeRI,	BspOVI,
DriI, Eam1105I, NruGI

EclRI Enterobacter cloacae R 5' CCCGGG
3' GGGCCC 5' ---C CCGGG--- 3'
3' ---GGGCC C--- 5' AhyI, Cfr9I, EaeAI, Pac25I, PspAI, PspALI, XcyI, XmaI, XmaCI

EclXI Enterobacter cloacae 590 5' CGGCCG
3' GCCGGC 5' ---C GGCCG--- 3'
3' ---GCCGG C--- 5' AaaI, BseX3I, BstZI, EagI, Eco52I, SenPT16I, XmaIII

Ecl18kI 2FQZ Enterobacter cloacae 18k 5' CCNGG
3' GGNCC 5' --- CCNGG--- 3'
3' ---GGNCC --- 5'

Ecl37kI Enterobacter cloacae 37k 5' CTGCAG
3' GACGTC 5' ---CTGCA G--- 3'
3' ---G ACGTC--- 5' AjoI, AliAJI, Ecl2zI, PaePI, Psp23I, PstI, Sag16I, SflI, YenI

Ecl2zI Enterobacter cloacae 2z 5' CTGCAG
3' GACGTC 5' ---CTGCA G--- 3'
3' ---G ACGTC--- 5' AliAJI, Bsp63I, CfrA4I, PaePI, Pfl21I, PstI, SflI, Sst12I, YenI

Eco24I Escherichia coli RFL24 5' GRGCYC
3' CYCGRG 5' ---GRGCY C--- 3'
3' ---C YCGRG--- 5'

Eco31I Escherichia coli RFL31 5' GGTCTC
3' CCAGAG 5' ---GGTCTCN NNNN--- 3'
3' ---CCAGAGNNNNN --- 5'

Eco32I Escherichia coli RFL32 5' GATATC
3' CTATAG 5' ---GAT ATC--- 3'
3' ---CTA TAG--- 5'

Eco47I Escherichia coli RFL47 5' GGWCC
3' CCWGG 5' ---G GWCC--- 3'
3' ---CCWG G--- 5' Bme18I, BsrAI, Csp68KI, ErpI, FssI, HgiEI, Kzo49I, SmuEI

Eco47III Escherichia coli RFL47 5' AGCGCT
3' TCGCGA 5' ---AGC GCT--- 3'
3' ---TCG CGA--- 5' AfeI, AitI, Aor51H, FunI

Eco52I Escherichia coli RFL52 5' CGGCCG
3' GCCGGC 5' ---C GGCCG--- 3'
3' ---GCCGG C--- 5' AaaI, BseX3I, BstZI, EagI, EclXI, SenPT16I, XmaIII

Eco56I Escherichia coli RFL56 5' GCCGGC
3' CGGCCG 5' ---G CCGGC--- 3'
3' ---CGGCC G--- 5'

Eco57I Escherichia coli RFL57 5' CTGAAG
3' GACTTC 5' ---CTGAAGN₁₂NNNN --- 3'
3' ---GACTTCN₁₂NN NN--- 5' AcuI, BspKT5I

Eco64I Escherichia coli RFL64 5' GGYRCC
3' CCRYGG 5' ---G GYRCC--- 3'
3' ---CCRYG G--- 5' BanI, BbvBI, BspT107I, BshNI, HgiCI, HgiHI, MspB4I, PfaAI

Eco72I Escherichia coli RFL72 5' CACGTG
3' GTGCAC 5' ---CAC GTG--- 3'
3' ---GTG CAC--- 5' AcvI, BcoAI, BbrPI, PmaCI, PmlI, PspCI

Eco78I Escherichia coli RFL78 5' GGCGCC
3' CCGCGG 5' ---GGC GCC--- 3'
3' ---CCG CGG--- 5'

Eco81I Escherichia coli RFL81 5' CCTNAGG
3' GGANTCC 5' ---CC TNAGG--- 3'
3' ---GGANT CC--- 5' AxyI, Bse21I, BspR7I, Bsu36I, Eco81I, MstII, OxaNI, SshAI

Eco88I Escherichia coli RFL88 5' CYCGRG
3' GRGCYC 5' ---C YCGRG--- 3'
3' ---GRGCY C--- 5' AquI, BcoI, BsiHKCI, BstSI, Eco27kI, NspSAI, PlaAI, PunAI

Eco91I Escherichia coli RFL91 5' GGTNACC
3' CCANTGG 5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5' Bse64I, BstEII, BstPI, BstT9I, BstT10I, EcaI, Eci125I, NspSAII

Eco105I Escherichia coli RFL105 5' TACGTA
3' ATGCAT 5' ---TAC GTA--- 3'
3' ---ATG CAT--- 5'

Eco130I Escherichia coli RFL130 5' CCWWGG
3' GGWWCC 5' ---C CWWGG--- 3'
3' ---GGWWC C--- 5'

Eco147I Escherichia coli RFL147 5' AGGCCT
3' TCCGGA 5' ---AGG CCT--- 3'
3' ---TCC GGA--- 5' AatI, AspMI, GdiI, PceI, Pme55I, SarI, Sru30DI, SseBI, SteI, StuI

Eco255I

Escherichia coli RFL255

5' AGTACT
3' TCATGA

5' ---AGT ACT--- 3'
3' ---TCA TGA--- 5'

Acc113I, AssI,	BmcAI, Bpa34I,
DpaI, RflFII, ScaI, ZrmI

Eco1831I

Escherichia coli RFL1831

5' CCSGG
3' GGSCC

5' --- CCSGG--- 3'
3' ---GGSCC --- 5'

AhaI, AseII, AsuC2I, BcnI,
BpuMI,	CauII, EcoHI, HgiS22I

EcoA4I Escherichia coli A4 5' GGTCTC
3' CCAGAG 5' ---GGTCTCN NNNN--- 3'
3' ---CCAGAGNNNNN --- 5'

EcoHI Escherichia coli HI 5' CCSGG
3' GGSCC 5' --- CCSGG--- 3'
3' ---GGSCC --- 5' AsuC2I, BcnI, BpuMI, CauII, EcoHI, HgiS22I, Kpn49kII, NciI

EcoHK31I Escherichia coli HK31 5' YGGCCR
3' RCCGGY 5' ---Y GGCCR--- 3'
3' ---RCCGG Y--- 5'

EcoICRI Escherichia coli 2bT 5' GAGCTC
3' CTCGAG 5' ---GAG CTC--- 3'
3' ---CTC GAG--- 5'

Eco75KI Escherichia coli BKM 5' GRGCYC
3' CYCGRG 5' ---GRGCY C--- 3'
3' ---C YCGRG--- 5'

Eco57MI Escherichia coli RFL57M 5' CTGRAG
3' GACYTC 5' ---CTGRAGN₁₂NNNN --- 3'
3' ---GACYTCN₁₂NN NN--- 5'

EcoNI Escherichia coli 5' CCTN₅AGG
3' GGAN₅TCC 5' ---CCTNN NNNAGG--- 3'
3' ---GGANNN NNTCC--- 5'

EcoO44I Escherichia coli O44 Hiromi 5' GGTCTC
3' CCAGAG 5' ---GGTCTCN NNNN--- 3'
3' ---CCAGAGNNNNN --- 5'

EcoO65I Escherichia coli K11a 5' GGTNACC
3' CCANTGG 5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5' AcrII, Bse64I, BstEII, BstPI, BstT9I, EcaI, Eco91I, EcoO128I

EcoO109I 1WTD Escherichia coli H709c 5' RGGNCCY
3' YCCNGGR 5' ---RG GNCCY--- 3'
3' ---YCCNG GR--- 5'

EcoO128I Escherichia coli O128Ly3 5' GGTNACC
3' CCANTGG 5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5' AspAI, Bse64I, BstEII, BstT9I, EcaI, EcoO65I, NspSAII, PspEI

EcoP15I Escherichia coli P15 5' CAGCAG
3' GTCGTC 5' ---CAGCAGN₂₅NN --- 3'
3' ---GTCGTCN₂₅ NN--- 5'

EcoR124II 2y3t , 2w74 Search Escherichia coli 5' 5'-GAAN₇RTCG-3' 5'

EcoRI 1QC9, Search EcoR1 structures Escherichia coli RY13 5' GAATTC
3' CTTAAG 5' ---G AATTC--- 3'
3' ---CTTAA G--- 5'

EcoRII 1NA6 Escherichia coli R245 5' CCWGG
3' GGWCC 5' --- CCWGG--- 3'
3' ---GGWCC --- 5' ApaORI, BseBI, BspNI, BstNI, BstOI, Bst2UI, MvaI, SspAI, ZanI

EcoRV 1RVE Search EcoRV Escherichia coli J62 pLG74 5' GATATC
3' CTATAG 5' ---GAT ATC--- 3'
3' ---CTA TAG--- 5'

EcoT14I Escherichia coli TB14 5' CCWWGG
3' GGWWCC 5' ---C CWWGG--- 3'
3' ---GGWWC C--- 5'

EcoT22I Escherichia coli TB22 5' ATGCAT
3' TACGTA 5' ---ATGCA T--- 3'
3' ---T ACGTA--- 5' BfrBI, Csp68KIII, Mph1103I, NsiI, PinBI, Ppu10I, SepI, SspD5II

EcoT38I Escherichia coli TH38 5' GRGCYC
3' CYCGRG 5' ---GRGCY C--- 3'
3' ---C YCGRG--- 5'

EcoVIII Escherichia coli E1585-68 5' AAGCTT
3' TTCGAA 5' ---A AGCTT--- 3'
3' ---TTCGA A--- 5'

Eco13kI Escherichia coli 13k 5' CCNGG
3' GGNCC 5' --- CCNGG--- 3'
3' ---GGNCC --- 5'

Eco21kI Escherichia coli 21k 5' CCNGG
3' GGNCC 5' --- CCNGG--- 3'
3' ---GGNCC --- 5'

Eco27kI Escherichia coli 27 5' CYCGRG
3' GRGCYC 5' ---C YCGRG--- 3'
3' ---GRGCY C--- 5' Ama87I, AvaI, Bse15I, BsoBI, BstSI, NspIII, OfoI, PunAI

Eco29kI Escherichia coli 5' CCGCGG
3' GGCGCC 5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'

Eco53kI Escherichia coli 53k 5' GAGCTC
3' CTCGAG 5' ---GAG CTC--- 3'
3' ---CTC GAG--- 5'

Eco137kI Escherichia coli 137k 5' CCNGG
3' GGNCC 5' --- CCNGG--- 3'
3' ---GGNCC --- 5'

EgeI Enterobacter gergoviae NA 5' GGCGCC
3' CCGCGG 5' ---GGC GCC--- 3'
3' ---CCG CGG--- 5'

EheI Erwinia herbicola 9/5 5' GGCGCC
3' CCGCGG 5' ---GGC GCC--- 3'
3' ---CCG CGG--- 5'

ErhI Erwinia rhaponici B9 5' CCWWGG
3' GGWWCC 5' ---C CWWGG--- 3'
3' ---GGWWC C--- 5'

ErhB9I Erwinia rhaponici B9 5' CGATCG
3' GCTAGC 5' ---CGAT CG--- 3'
3' ---GC TAGC--- 5' Afa16RI, Afa22MI, EagBI, NblI, Ple19I, Psu161I, RshI, XorII

ErhB9II Erwinia rhaponici B9 5' CCWWGG
3' GGWWCC 5' ---C CWWGG--- 3'
3' ---GGWWC C--- 5'

ErpI Erwinia rhaponici 5' GGWCC
3' CCWGG 5' ---G GWCC--- 3'
3' ---CCWG G--- 5' Bme18I, BthAI, Csp68KI, Eco47I, FssI, HgiEI, Kzo49I, SmuEI

EsaBC3I Environmental sample BC3 5' TCGA
3' AGCT 5' ---TC GA--- 3'
3' ---AG CT--- 5'

EsaBC4I Environmental sample BC4 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

EspI Eucapsis sp. 5' GCTNAGC
3' CGANTCG 5' ---GC TNAGC--- 3'
3' ---CGANT CG--- 5'

Esp3I Erwinia sp. RFL3 5' CGTCTC
3' GCAGAG 5' ---CGTCTCN NNNN--- 3'
3' ---GCAGAGNNNNN --- 5'

Esp4I Erwinia sp. RFL4 5' CTTAAG
3' GAATTC 5' ---C TTAAG--- 3'
3' ---GAATT C--- 5'

Esp1396I Enterobacter sp. RFL1396 5' CCAN₅TGG
3' GGTN₅ACC 5' ---CCANNNN NTGG--- 3'
3' ---GGTN NNNNACC--- 5' AccB7I, AcpII, Asp10HII, BasI, PflBI, PflMI, Van91I

F

Enzyme PDB code Source Recognition sequence Cut Isoschizomers

FalI Flavobacterium aquatile Ob10 5' AAGN₅CTT
3' TTCN₅GAA 5' ---AAGN₅CTTN₇NNNNNN --- 3'
3' ---TTCN₅GAAN₇N NNNNN--- 5'

FalII Flavobacterium aquatile Ob10 5' CGCG
3' GCGC 5' ---CG CG--- 3'
3' ---GC GC--- 5' BepI, BspFNI, Bsp123I, BspFNI, BstUI, BtkI, FalII, SelI, ThaI

FaqI Flavobacterium aquatile RFL1 5' GGGAC
3' CCCTG 5' ---GGGACN₈NN NNNN--- 3'
3' ---CCCTGN₈NNNNNN --- 5'

FatI Flavobacterium aquatile NL3 5' CATG
3' GTAC 5' --- CATG--- 3'
3' ---GTAC --- 5'

FauI Flavobacterium aquatile 5' CCCGC
3' GGGCG 5' ---CCCGCNNNN NN--- 3'
3' ---GGGCGNNNNNN --- 5'

FauBII Flavobacterium aureus B 5' CGCG
3' GCGC 5' ---CG CG--- 3'
3' ---GC GC--- 5' Bpu95I, BspFNI, Bsp123I, BspFNI, BstUI, BtkI, Csp68KVI, SelI, ThaI

FauNDI Flavobacterium aquatile ND 5' CATATG
3' GTATAC 5' ---CA TATG--- 3'
3' ---GTAT AC--- 5'

FbaI

Flavobacterium balustinum

5' TGATCA
3' ACTAGT

5' ---T GATCA--- 3'
3' ---ACTAG T--- 5'

AbaI, BclI, BsiQI,
BspXII, BstT7I, Ksp22I,	ParI

FblI Flavobacterium balustinum 5' GTMKAC
3' CAKMTG 5' ---GT MKAC--- 3'
3' ---CAKM TG--- 5' AccI, XmiI

FbrI Flavobacterium breve 5' GCNGC
3' CGNCG 5' ---GC NGC--- 3'
3' ---CGN CG--- 5'

FdiI Fremyella diplosiphon 5' GGWCC
3' CCWGG 5' ---G GWCC--- 3'
3' ---CCWG G--- 5' Bme18I, BthAI, Csp68KI, ErpI, FssI, HgiEI, Kzo49I, SmuEI

FdiII Fremyella diplosiphon 5' TGCGCA
3' ACGCGT 5' ---TGC GCA--- 3'
3' ---ACG CGT--- 5' Acc16I, AosI, AviII, FspI, MstI, NsbI, PamI, Pun14627I

FgoI Fervidobacterium gondwanense AB39T 5' CTAG
3' GATC 5' ---C TAG--- 3'
3' ---GAT C--- 5'

FmuI Flavobacterium multivorum 5' GGNCC
3' CCNGG 5' ---GGNC C--- 3'
3' ---C CNGG--- 5' FmuI AvcI, BavAII, Bce22I, Bsu54I, NspIV, Pde12I, UnbI

FnuAI Fusobacterium nucleatum A 5' GANTC
3' CTNAG 5' ---G ANTC--- 3'
3' ---CTNA G--- 5'

FnuCI Fusobacterium nucleatum C 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' Bme12I, Bsp67I, Bst19II, CcyI, FnuEI, MgoI, NphI, SauMI

FnuDI Fusobacterium nucleatum D 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

FnuDII Fusobacterium nucleatum D 5' CGCG
3' GCGC 5' ---CG CG--- 3'
3' ---GC GC--- 5' Bpu95I, Bsh1236I, Bsp50I, BstFNI, BstUI, Csp68KVI, FnuDII, ThaI

FnuDIII Fusobacterium nucleatum D 5' GCGC
3' CGCG 5' ---GCG C--- 3'
3' ---C GCG--- 5' AspLEI, BspLAI, BstHHI, CfoI, HhaI, Hin6I, HinP1I, HspAI, SciNI

FnuEI Fusobacterium nucleatum E 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' Bme12I, Bsp67I, Bst19II, CcyI, FnuCI, MgoI, NphI, Sau3AI

Fnu4HI Fusobacterium nucleatum 4H 5' GCNGC
3' CGNCG 5' ---GC NGC--- 3'
3' ---CGN CG--- 5'

FokI 2FOK Flavobacterium okeanokoites 5' GGATG
3' CCTAC 5' ---GGATGN₈N NNNN--- 3'
3' ---CCTACN₈NNNNN --- 5'

FriOI Flavobacterium sp. O9 5' GRGCYC
3' CYCGRG 5' ---GRGCY C--- 3'
3' ---C YCGRG--- 5'

FseI Frankia sp. Eu11b 5' GGCCGGCC
3' CCGGCCGG 5' ---GGCCGG CC--- 3'
3' ---CC GGCCGG--- 5'

FsiI

Frankia sp.

5' RAATTY
3' YTTAAR

5' ---R AATTY--- 3'
3' ---YTTAA R--- 5'

AcsI, ApoI,

CfaI,

XapI

FspI Fischerella sp. 5' TGCGCA
3' ACGCGT 5' ---TGC GCA--- 3'
3' ---ACG CGT--- 5' Acc16I, AosI, AviII, FdiII, MstI, NsbI, PamI, Pun14627I

FspII Fischerella sp. 5' TTCGAA
3' AAGCTT 5' ---TT CGAA--- 3'
3' ---AAGC TT--- 5' Asp10HI, Bim19I, BsiCI, BstBI, Csp45I, LspI, PlaII, Ssp1I, SviI

Fsp1604I Flavobacterium sp. I 16-04 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, EcoRII, SleI

FspAI Flexibacter sp. TV-m21K 5' RTGCGCAY
3' YACGCGTR 5' ---RTGC GCAY--- 3'
3' ---YACG CGTR--- 5'

FspBI Flavobacterium sp. RFLI 5' CTAG
3' GATC 5' ---C TAG--- 3'
3' ---GAT C--- 5'

Fsp4HI Flavobacterium sp. 4H 5' GCNGC
3' CGNCG 5' ---GC NGC--- 3'
3' ---CGN CG--- 5'

FspMSI Fischerella sp. 5' GGWCC
3' CCWGG 5' ---G GWCC--- 3'
3' ---CCWG G--- 5' Bme18I, BthAI, DsaIV, ErpI, HgiBI, HgiEI, Kzo49I, SmuEI

FssI Fibrobacter succinogenes S85 5' GGWCC
3' CCWGG 5' ---G GWCC--- 3'
3' ---CCWG G--- 5' Bme18I, BthAI, DsaIV, ErpI, FspMSI, HgiEI, Kzo49I, VpaK11AI

FunI Fischerella uniformis 5' AGCGCT
3' TCGCGA 5' ---AGC GCT--- 3'
3' ---TCG CGA--- 5' AfeI, AitI, Aor51H, Eco47III

FunII Fischerella uniformis 5' GAATTC
3' CTTAAG 5' ---G AATTC--- 3'
3' ---CTTAA G--- 5'

Notes

↑ Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.
↑ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. doi:10.1093/nar/gkg274. PMC 152790 . PMID 12654995.
↑ Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN 0-7167-4684-0.
↑ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID 4124164.
↑ Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene. 92 (1–2): 1–248. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.
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v t e Restriction modification system: restriction enzyme
Basic Concept	Isoschizomer Neoschizomer Isocaudomer
Recognition sequence 4bp	TaqI Sau3A AluI* HaeIII* DpnII NlaIII HpaII
Recognition sequence 5bp	EcoRII FokI
Recognition sequence 6bp	AaaI BglII PovII* EcoRV* EcoRI BamHI HindIII XbaI XhoI SacI PstI NdeI
Recognition sequence 8bp	NotI
Lists	A Ba–Bc Bd–Bp Bsa–Bso Bsp–Bss Bst–Bv C-D E-F G-K L-N O-R S T-Z
* means cleavage produces blunt ends

List of restriction enzyme cutting sites: E–F

Contents

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Restriction enzymes

E

F

Notes

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