Mansonella ozzardi | |
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Scientific classification | |
Domain: | Eukaryota |
Kingdom: | Animalia |
Phylum: | Nematoda |
Class: | Chromadorea |
Order: | Rhabditida |
Family: | Onchocercidae |
Genus: | Mansonella |
Species: | M. ozzardi |
Binomial name | |
Mansonella ozzardi Manson, 1897 | |
Mansonella ozzardi is a filarial (arthropod-borne) nematode (roundworm). This filarial nematode is one of two that causes serous cavity filariasis in humans. The other filarial nematode that causes it in humans is Mansonella perstans . M. ozzardi is an endoparasite that inhabits the serous cavity of the abdomen in the human host. It lives within the mesenteries, peritoneum, and in the subcutaneous tissue.
Like other nematodes, M. ozzardi is a cylindrical and bilaterally symmetrical worm, with a pseudocoel, or a false body cavity. The exterior of the parasite, the cuticle, is a protective layer that can withstand the harsh environment in the digestive tracts of the human hosts.
Mansonella ozzardi and other nematodes have longitudinal muscles that run along the body wall. They also have dorsal, ventral, and longitudinal nerve cords connected to these longitudinal muscles.
In the adult stages of M. ozzardi, the females are larger than the males.
Mansonella ozzardi is usually smaller than those Onchocerca Volvulus (that causes human onchocerciasis) when both species co-exist; especially in some rural areas in Brazil, within the Amazonian onchocerciasis focus.
This is a dioecious species; the female is believed to release a pheromone to attract males. [1] When the male finds the female, he will coil around the female over the genital pore. The male's spicules are used to hold the female during copulation. The females are ovoviviparous. The sperm of a nematode lacks a flagellum. Its motility is due to its amoeboid-type cell.
This is a New World parasite. It is prominent in the subtropical, tropical, and temperate regions of Central and South America (Mexico, Panama, Brazil, Colombia, and Argentina), and the Caribbean. [2] The prevalence of M. ozzardi in Brazil is very high; about 44–52% of Brazilian farmers are infected. It is also prevalent with the American Indian population. Many transmission hotspots in the Amazon Basin map to indigenous communities. Endemicity levels vary widely among rural villages, situated a few kilometers apart along the same rivers in the western Amazon Basin of Brazil. [3]
Mansonella ozzardi is transmitted by two types of arthropods that feed on the blood of humans: biting midges (genus Culicoides ) and blackflies (genus Simulium ). In the Caribbean, M. ozzardi uses the biting midge as its intermediate host. The black fly serves as the intermediate host for the filariae parasite in the Amazon Basin and mainland South America.
The biting midges are small flies that breed on sandy beaches near the sea. Some species also lay their eggs on decaying leaf litter, humus, manure, and partially rotten vegetation, in tree holes, and the cut stumps of banana trees. They feed on the blood of mammals, birds, reptiles, and humans. Their short mouthparts prohibit them from biting through clothing. As a result, they prefer to feed on the head or other exposed body parts. Blood meals occur throughout the day and night, but biting activity peaks during the early evening. Since individual bites can be painful, they are a cause of concern because they tend to attack in swarms of hundreds or thousands. Due to their small size, they can pass through mesh mosquito nets.
The blackflies are larger blood-sucking flies that breed in fast-flowing streams and rivers. Their larvae can be found attached to submerged vegetation and stones of infected waterways. Blood meals occur during the day in the outdoors, especially along riverbeds. After a meal, the flies rest for 2–3 days on trees and other vegetation to digest the blood. Like the biting midges, blackflies attack their victims in swarms and their bites are painful. The bites may cause swelling, inflammation, and irritation that may last for weeks. [4]
The pathogenicity of M. ozzardi needs further research. Although the adult worms live in the body cavities and the mesentery, they do cause clinical manifestations to their human hosts.
Mansonella ozzardi is also considered as a possible cause for corneal lesions. Even though there is no significant evidence for it, but in certain regions in Central and South America where both M. ozzardi and O. volvulus co-occur, skin biopsies have always showed that onchoserciasis is the cause for corneal lesions.
Microscopic examination is the most practical diagnostic tool used to identify the M. ozzardi microfilariae in blood samples from infected patients. Blood smears are usually stained with haematoxylin or Giemsa to visualize the worms under the microscope. [5]
Diagnosticians must not rely entirely on blood samples, since microfilariae have also been detected in the skin. Ultrasound may be used to detect the presence of the adult worms. Researchers are currently developing a polymerase chain reaction-based method of detecting the parasites in skin biopsies. [6]
Because M. ozzardi harbors the endosymbiotic bacteria Wolbachia, doxycycline may be an effective therapy to eliminate adult worms. However, no trials with doxycycline (either alone or in combination with ivermectin) have been conducted for M. ozzardi infection.
Ivermectin is the treatment of choice for M. ozzardi infections. It is a potent macrocyclic lactone that binds to chloride channels, which then open and allow chloride ions to enter the affected cells. These cells hyperpolarize, resulting in muscle paralysis in the M. ozzardi microfilariae. This allows host immune cells to adhere to the microfilariae surface and facilitate their elimination. Ivermectin is unable to kill the adult worms. [7]
Diethylcarbamazine (DEC) also has been used in treating filarial infections. While DEC works well against many filarial parasites, it is not effective in treating M. ozzardi microfilarial infections. [8]
Aside from ivermectin, preventive measures can be taken by individuals living in areas endemic to M. ozzardi. Since biting midges cannot bite through clothing, those living in the Caribbean should wear long-sleeved shirts and pants to decrease the body parts exposed. Insect repellents could also be used to cover body parts not protected by clothing. [9] Communities should also maintain the natural vegetation around them to decrease the possible breeding grounds for the biting midges.
In South America, the best way to control the population of blackflies, and the transmission of M. ozzardi, is to apply insecticides specific for blackfly larvae to streams and rivers. Application of insecticide to a specific breeding site also kills larvae in breeding sites up to 10 km downstream. Extensive networks of waterways must be treated with insecticide since blackflies have the ability to fly with the wind for several hundreds of kilometers. Individuals should also avoid the waterways whenever possible.
Loa loa filariasis is a skin and eye disease caused by the nematode worm Loa loa. Humans contract this disease through the bite of a deer fly or mango fly, the vectors for Loa loa. The adult Loa loa filarial worm migrates throughout the subcutaneous tissues of humans, occasionally crossing into subconjunctival tissues of the eye where it can be easily observed. Loa loa does not normally affect one's vision but can be painful when moving about the eyeball or across the bridge of the nose. The disease can cause red itchy swellings below the skin called "Calabar swellings". The disease is treated with the drug diethylcarbamazine (DEC), and when appropriate, surgical methods may be employed to remove adult worms from the conjunctiva. Loiasis belongs to the so-called neglected diseases.
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Mansonella perstans is a filarial (arthropod-borne) nematode (roundworm), transmitted by tiny blood-sucking flies called midges. Mansonella perstans is one of two filarial nematodes that causes serous cavity filariasis in humans. The other filarial nematode is Mansonella ozzardi. M. perstans is widespread in many parts of sub-Saharan Africa, parts of Central and South America, and the Caribbean.
Mansonelliasis is the condition of infection by the nematode Mansonella. The disease exists in Africa and tropical Americas, spread by biting midges or blackflies. It is usually asymptomatic.
Brugia timori is a filarial (arthropod-borne) nematode (roundworm) which causes the disease "Timor filariasis", or "Timorian filariasis". While this disease was first described in 1965, the identity of Brugia timori as the causative agent was not known until 1977. In that same year, Anopheles barbirostris was shown to be its primary vector. There is no known animal reservoir host.
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