N-acyl-D-amino-acid deacylase

N-acyl-D-amino-acid deacylase
Identifiers
EC no.	3.5.1.81
CAS no.	65979-42-2
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a N-acyl-D-amino-acid deacylase (EC 3.5.1.81) is an enzyme that catalyzes the chemical reaction

N-acyl-D-amino acid + H₂O ${\displaystyle \rightleftharpoons }$ an acid + D-amino acid

Thus, the two substrates of this enzyme are N-acyl-D-amino acid and H₂O, whereas its two products are acid and D-amino acid.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-acyl-D-amino acid amidohydrolase. It employs one cofactor, zinc.

Structural studies

As of late 2007, 8 structures have been solved for this class of enzymes, with PDB accession codes 1M7J, 1RJP, 1RJQ, 1RJR, 1RK5, 1RK6, 1V4Y, and 1V51.

References

• Wakayama M, Katsuno Y, Hayashi S, Miyamoto Y, Sakai K, Moriguchi M (1995). "Cloning and sequencing of a gene encoding D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 and expression of the gene in Escherichia coli". Biosci. Biotechnol. Biochem. 59 (11): 2115–9. doi: 10.1271/bbb.59.2115 . PMID   8541651.
• Wakayama M, Hayashi S, Yatsuda Y, Katsuno Y, Sakai K, Moriguchi M (1996). "Overproduction of D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 in Escherichia coli and its purification". Protein Expr. Purif. 7 (4): 395–9. doi:10.1006/prep.1996.0059. PMID   8776758.