Potato mop-top virus

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Potato mop-top virus
Virus classification OOjs UI icon edit-ltr.svg
(unranked): Virus
Realm: Riboviria
Kingdom: Orthornavirae
Phylum: Kitrinoviricota
Class: Alsuviricetes
Order: Martellivirales
Family: Virgaviridae
Genus: Pomovirus
Species:
Potato mop-top virus

Potato mop-top virus (PMTV) is a plant pathogenic virus transmitted through the vector Spongospora subterranea that affects potatoes. PMTV belongs to family of Virgaviridae, [1] and the genus Pomovirus (Potato mop-top virus). [2] The virus was first identified in 1966 by Calvert and Harrison in Britain, [3] and is now reported in many other potato cultivating regions of the world including U.S.A., Canada, China, Pakistan, Japan, South American countries and many parts of Europe. [4] Many disease management systems have been found to be ineffective against the virus, although a combination of sanitation and vector controls seems to work well.

Contents

Hosts and symptoms

Tuber flesh Tuber Flesh.png
Tuber flesh

As the name implies, the main host is potato; however, this virus also affects some common vegetable plants and weeds. Some of them include tomato, black nightshade, lambsquarters and ground cherry. [5] The potato mop top virus' primary hosts are plants in the Solanaceae and Chenopodiaceae. [6] Potatoes that are infected by PMTV generally show hollow necrotic spots on the inside. [7] This virus shows different symptoms in Europe; for example, the infected plants usually show dark brown necrotic arcs that discolor the tuber's flesh (these symptoms are very similar to those caused by alfalfa mosaic virus). [5] Plants showing no symptoms of PMTV tend to produce larger quantities of infected tubers if they are derived from plants that have shown foliar symptoms in the previous year. [8]

Disease cycle

The spores that PMTV is vectored in can live in the soil for up to 18 years giving the virus a long period of survival. The critical period for infection of S. subterranea and consequently PMTV is earlier in the potato growth cycle, during stolon formation and tuber set, which lasts 3–4 weeks. [9] The disease cycle of PMTV begins with the virus entering the host plant's cell and disassembling its capsid to release the viral RNA into the cell. [10] As a pomovirus, PMTV uses the host plant's machinery for replication and translation which both follow positive-stranded RNA models. After replicates of the viral RNA and of the capsid proteins are made in the cytosol, the virus reassembles itself and exits the cell to infect other cells. Because it is vectored by a protist, it generally appears in cooler and more moist times of the year.[ citation needed ]

The naturally occurring virus has been found to have systemic effects on Nicotiana benthamiana. However, when the gene for TRIPLE GENE BLOCK1 (TGB1), which is a movement factor protein, was silenced, the systemic movement of the virus was hindered. [11] The virus moves through the xylem to infect plants systemically, but can also spread locally through cell-to-cell movement. In host plants, the infected tissues include both leaves and the cytoplasm. [12] It has been shown that PMTV infected seeds are planted, they only partially infect the following generations of plants, showing that its vector, S. subterranea is very important for transmission. [9]

Potato Mop Top Virus Potato mop top virus 2012.jpg
Potato Mop Top Virus

Environment

The vector for PMTV, S. subterranea , is a slime mold known to cause powdery scab disease that favors wet and humid conditions, specifically poorly drained soil. [13] In fact, this disease doesn’t occur often in areas with less than 30 inches of rain per year. The chance of disease onset increases in areas that get more than 45 inches of rain per year. Such moist environments helps facilitate the vector's zoospore movement to the infection sites (roots and tubers). It is thought that powdery scab disease development increases when high soil moisture gradually dries out, as this increases zoospore germination. S. subterranea tends to prefer more acidic soil, ranging from pHs of 4.7 to 7.6. S. subterranea thrives in temperate conditions, between 52 and 75 degrees Fahrenheit, with the optimal temperature for infection by the vector being 60 degrees. [14]

Disease development can also be encouraged by certain agricultural practices. For example, increased use of nitrate or ammonium nitrogen containing fertilizers increases the incidence and severity of powdery scab disease, caused by the PMTV vector S. subterranea . [15] This can be due to the fact that fertilization enhances root growth, which provides a larger amount of tissue that can be infected.[ citation needed ]

Management

Chemical treatments for field application against viruses are currently not available. This holds especially true for potatoes, because once infected by a virus, they will stay infected for the remainder of their lives. Thus, preventative methods are more viable options. This includes methods such as resistance-breeding, vector management, and crop sanitation. [16]

Resistance-breeding, or generating plants that are genetically resistant to pathogen infections, is another option being explored. Generating vector-resistant plants have been proven to be largely unsuccessful for PMTV. This is because plants need to have immunity towards S. subterranea in its tubers, roots, and stolons in order to completely resist infection by the vector and virus. To this date, however, potatoes have been produced with resistant tubers but susceptible roots. Although resistance breeding has not yet provided benefits for commercially available PMTV varieties, there have been some promising results in ongoing research. There are some partially resistant varieties of commercial crop that are available now. [15] An example is NY99, a breeding line that has shown a low incidence of PMTV-infected tubers. Resistance-breeding, if successful, would prevent the virus from starting its pathogenesis. [16]

Vector management concentrates its efforts to reduce the level of S. subterranea in the soil as a way to combat the virus. Soil treatments, such as fungicides containing fluazinam, have been shown to be partially effective at reducing numbers of viable S. subterranea spores available to germinate into zoospores. [5] Another form of vector management is crop rotations with brassicas and datura (a weed). Rotating with these plants has shown to produce low levels of S. subterranea in the soil. [14]

Proper crop sanitation is achieved by planting certified seed potatoes. It is crucial that these seed potatoes come from production areas that have had no previous contact with either the vector or the virus itself. Further risk reduction can be achieved by properly sanitizing all machinery that has come in contact with soil and plant debris. [5]

Importance

PMTV is a relatively new discovery, having first been reported in Britain in 1966, and then in the U.S. in 2002. [14] The vector S. subterranea, however, has been around for over 150 years, being first discovered in Germany in 1841. It was first discovered in the U.S. in 1913.[ citation needed ]

PMTV can lead to a very significant loss in the yield of potatoes. For example, a study conducted on Scottish seed potatoes showed a yield reduction as great as 67%. [13] The virus also causes spraing and necrosis in the flesh of tubers. These symptoms are mainly morphological defects that yield unaesthetic potatoes which face commercial rejection from processors and packers. If potatoes exported to other countries are found to be infected, it can negatively affect agricultural trading. For example, Maine was quarantined due to its fairly recent outbreaks. [13] In addition to economic losses, the presence of PMTV can also negatively impact the reputation of a country (or region, state, or farm) for other exports.[ citation needed ]

Genome

PMTV is a (+)ssRNA virus with a tripartite genome. The longest segment of PMTV, RNA-rep encodes RNA dependent RNA polymerase and replicase subunits. [17] The second segment, RNA-CP codes for virus coat protein (CP), and CP-RT or minor CP, which is produced by translational read-through of the CP stop codon. [18] The third segment, RNA-TGB encodes triple gene block of movement proteins, TGB1, TGB2, TGB3, [19] and 8K protein which is a viral suppressor of RNA silencing. [20]

Pathogenesis

The vector for PMTV is a protist, S. subterranea . [21] As S. subterranea infects the roots of potatoes, the virus is given access to the root cells and starts its habitation of the plant. By taking control of the cell's cellular machinery, the virus can replicate. The virus then moves onto more potato cells and spreads systemically through the plant. The systemic movement of PMTV is facilitated in large by a protein named TRIPLE GENE BLOCK1 (TGB1) which has an internal domain spurring transportation of the virus to neighboring cells, and an N-terminal domain used for long-distance (systemic) travel. This N-terminal domain of TGB1 has shown some promise as a target to limit systemic infection of the pathogen in N. benthamiana , since systemic infection requires an interaction between a molecule called importin-a within the host plant and the N-terminal domain. [11] Of the three positive-sense RNA molecules used by PMTV, there is one thought to code for a virulence factor protein. The other two molecules code for a polymerase and a coat protein both of which are necessary for the survival of the virus. RNA-TGB is a gene with 4 open reading frames (ORFs); the first three overlapping ORFs form an area called the triple gene block. This 3 frame block codes for essential movement factor proteins that facilitate cell-to-cell movement. The fourth ORF codes for another cysteine-rich protein that increases virulence and has some RNA silencing suppressive activity. [22]

See also

Related Research Articles

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<i>Tobacco mosaic virus</i> Infects tomato family, beans, flowers...

Tobacco mosaic virus (TMV) is a positive-sense single-stranded RNA virus species in the genus Tobamovirus that infects a wide range of plants, especially tobacco and other members of the family Solanaceae. The infection causes characteristic patterns, such as "mosaic"-like mottling and discoloration on the leaves. TMV was the first virus to be discovered. Although it was known from the late 19th century that a non-bacterial infectious disease was damaging tobacco crops, it was not until 1930 that the infectious agent was determined to be a virus. It is the first pathogen identified as a virus. The virus was crystallised by W.M. Stanley. It has a similar size to the largest synthetic molecule, known as PG5.

<span class="mw-page-title-main">Plant virus</span> Virus that affects plants

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<i>Brome mosaic virus</i> Species of virus

Brome mosaic virus (BMV) is a small, positive-stranded, icosahedral RNA plant virus belonging to the genus Bromovirus, family Bromoviridae, in the Alphavirus-like superfamily.

The potato spindle tuber viroid (PSTVd) was the first viroid to be identified. PSTVd is a small, single stranded circular RNA molecule closely related to the chrysanthemum stunt viroid. Present within the viroidal RNA is the Pospiviroid RY motif stem loop common to its genus. The natural hosts are potatoes and tomatoes. All potatoes and tomatoes are susceptible to PSTVd and there is no form of natural resistance. Natural infections have also been seen in avocados and infections in other solanaceous crops have been induced in the laboratory. Until 2017 PSTVd was thought to be unable to infect Solanum sisymbriifolium. Then in May seeds exported by a Dutch company were noticed to be infected. These seeds were shipped from the company, but had been originally bred to their specifications in two Asian countries. Pstv also causes Tomato bunchy top and is seed transmitted in tomato.

<i>Tobamovirus</i> Genus of viruses

Tobamovirus is a genus of positive-strand RNA viruses in the family Virgaviridae. Many plants, including tobacco, potato, tomato, and squash, serve as natural hosts. Diseases associated with this genus include: necrotic lesions on leaves. The name Tobamovirus comes from the host and symptoms of the first virus discovered.

<span class="mw-page-title-main">Spraing</span>

Spraing is a collective term for diseases and disorders of potato tubers that cause brown streaks in the flesh of the potato. Spraing can be caused by two viruses, tobacco rattle virus (TRV) which is transmitted by trichodorid nematodes, or Potato mop-top virus (PMTV) transmitted by the powdery scab fungus Spongospora subterranea. The two viruses may have synergistic effects in disease progression when both are present. Spraing like symptoms can also be caused by physiological defects or specific nutrient deficiencies, most notably Calcium.

<i>Tomato bushy stunt virus</i> Species of virus

Tomato bushy stunt virus (TBSV) is a virus of the tombusvirus family. It was first reported in tomatoes in 1935 and primarily affects vegetable crops, though it is not generally considered an economically significant plant pathogen. Depending upon the host, TBSV causes stunting of growth, leaf mottling, and deformed or absent fruit. The virus is likely to be soil-borne in the natural setting, but can also be transmitted mechanically, for example through contaminated cutting tools. TBSV has been used as a model system in virology research on the life cycle of plant viruses, particularly in experimental infections of the model host plant Nicotiana benthamiana.

<i>Alfalfa mosaic virus</i> Species of virus

Alfalfa mosaic virus (AMV), also known as Lucerne mosaic virus or Potato calico virus, is a worldwide distributed phytopathogen that can lead to necrosis and yellow mosaics on a large variety of plant species, including commercially important crops. It is the only Alfamovirus of the family Bromoviridae. In 1931 Weimer J.L. was the first to report AMV in alfalfa. Transmission of the virus occurs mainly by some aphids, by seeds or by pollen to the seed.

<span class="mw-page-title-main">Powdery scab</span>

Powdery scab is a disease of potato tubers. It is caused by the cercozoan Spongospora subterranea f. sp. subterranea and is widespread in potato growing countries. Symptoms of powdery scab include small lesions in the early stages of the disease, progressing to raised pustules containing a powdery mass. These can eventually rupture within the tuber periderm. The powdery pustules contain resting spores that release anisokont zoospores to infect the root hairs of potatoes or tomatoes. Powdery scab is a cosmetic defect on tubers, which can result in the rejection of these potatoes. Potatoes which have been infected can be peeled to remove the infected skin and the remaining inside of the potato can be cooked and eaten.

Rice hoja blanca tenuivirus (RHBV), Spanish for "white leaf rice virus", is a plant virus in the family Phenuiviridae. RHBV causes Hoja blanca disease (HBD), which affects the leaves of the rice plant Oryza sativa, stunting the growth of the plant or killing it altogether. RHBV is carried by an insect vector, Tagosodes orizicolus, a type of planthopper. The virus is found in South America, Mexico, throughout Central America, the Caribbean region, and the southern United States. In South America, the disease is endemic to Colombia, Venezuela, Ecuador, Peru, Suriname, French Guiana and Guyana.

<i>Cymbidium mosaic virus</i> Species of virus

Cymbidium mosaic virus (CymMV) is a plant pathogenic virus of the family Alphaflexiviridae.

Potato virus Y (PVY) is a plant pathogenic virus of the family Potyviridae, and one of the most important plant viruses affecting potato production.

Soil-borne wheat mosaic virus is a rod-shaped plant pathogen that can cause severe stunting and mosaic in susceptible wheat, barley and rye cultivars. The disease has often been misdiagnosed as a nutritional problem, but this has actually allowed in part for the fortuitous visual selection by breeding programs of resistant genotypes. Soil-borne wheat mosaic virus is part of the genus Furovirus. Members of this genus are characterized by rigid rod-shaped particles and positive sense RNA genomes consisting of two molecules that are packaged into separate particles that code for either replication, mobility, structure or defense against the host. The virus is spread by a fungal-like protist, Polymyxa graminis, whose asexual secondary and sexual primary cycles help the virus spread. The disease produces secondary symptoms from the root cell infection. The disease is a serious contributor to loss in crop yield.

Sweet potato feathery mottle virus (SPFMV) is a member of the genus Potyvirus in the family Potyviridae. It is most widely recognized as one of the most regularly occurring causal agents of sweet potato viral disease (SPVD) and is currently observed in every continent except Antarctica. The number of locations where it is found is still increasing; generally, it is assumed that the virus is present wherever its host is. The virus has four strains that are found in varying parts of the world.

Pomovirus is a genus of viruses, in the family Virgaviridae. Plants and dicotyledons serve as natural hosts. There are five species in this genus. Diseases associated with this genus include: dwarfing of shoots (mop-top) and potato spraing disease. The name of the genus is derived from Potato mop-top virus, Potato mop-top virus, giving rise to Pomovirus.

<i>Potexvirus</i> Genus of viruses

Potexvirus is a genus of pathogenic viruses in the order Tymovirales, in the family Alphaflexiviridae. Plants serve as natural hosts. There are 48 species in this genus, three of which are assigned to a subgenus. Diseases associated with this genus include: mosaic and ringspot symptoms. The genus name comes from POTato virus X).

<i>Carlavirus</i> Genus of viruses

Carlavirus, formerly known as the "Carnation latent virus group", is a genus of viruses in the order Tymovirales, in the family Betaflexiviridae. Plants serve as natural hosts. There are 53 species in this genus. Diseases associated with this genus include: mosaic and ringspot symptoms.

<span class="mw-page-title-main">Viral diseases of potato</span>

Viral diseases of potato are a group of diseases caused by different types of viruses that affect potato crops worldwide and, although they do not affect human or animal health since they are viruses that only infect vegetables, they are a source of great economic losses annually. About 28 viruses have been reported infecting potato crops. However, potato virus X (PVX), potato virus Y (PVY), and potato leafroll virus (PLRV) are the most important viruses worldwide. Some others are of economic importance only in some regions. Such is the case of potato virus M (PVM) in some Asian and European countries.

References

  1. ↑ Adams MJ, Antoniw JF, Kreuze J (2009-10-28). "Virgaviridae: a new family of rod-shaped plant viruses". Archives of Virology. 154 (12): 1967–72. doi: 10.1007/s00705-009-0506-6 . PMID   19862474.
  2. ↑ "Genus: Pomovirus - Virgaviridae - Positive-sense RNA Viruses - International Committee on Taxonomy of Viruses (ICTV)". International Committee on Taxonomy of Viruses (ICTV). Retrieved 2018-06-14.[ dead link ]
  3. ↑ Calvert EL, Harrison BD (September 1966). "Potato Mop-Top, a Soil-Borne Virus". Plant Pathology. 15 (3): 134–139. doi:10.1111/j.1365-3059.1966.tb00333.x. ISSN   0032-0862.
  4. ↑ Beuch U (2013). Distribution and diversity of Potato mop-top virus in Sweden. Sveriges lantbruksuniversitet. Institutionen för växtbiologi och skogsgenetik. Uppsala: Dept. of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences. ISBN   9789157678935. OCLC   940749818.
  5. 1 2 3 4 "Wisconsin Pest Survey Fact Sheet: Potato Mop Top Virus" (PDF). Wisconsin Department of Agriculture, Trade and Consumer Protection.
  6. ↑ Robinson, Andy. "Potato Tuber Viruses: Mop-top Management" (PDF).
  7. ↑ Mallik I, Gudmestad NC (2014-10-10). "First Report of Potato mop top virus Causing Potato Tuber Necrosis in Colorado and New Mexico". Plant Disease. 99 (1): 164. doi: 10.1094/PDIS-08-14-0819-PDN . ISSN   0191-2917. PMID   30699762.
  8. ↑ Carnegie SF, Cameron AM, McCreath M (2010-06-05). "Foliar Symptoms Caused by Potato mop-top virus on Potato Plants During Vegetative Propagation in Scotland and Their Association With Tuber Yield, Spraing and Tuber Infection". Potato Research. 53 (2): 83–93. doi:10.1007/s11540-010-9153-2. ISSN   0014-3065. S2CID   25557894.
  9. 1 2 Directorate, Government of Canada, Canadian Food Inspection Agency, Plant Health Science (2012-01-25). "Potato mop-top virus (PMTV) - Fact Sheet". www.inspection.gc.ca. Retrieved 2015-11-11.{{cite web}}: CS1 maint: multiple names: authors list (link)
  10. ↑ Agrios, George (2005). Plant Pathology. Amsterdam: Elsevier Academic.
  11. 1 2 Lukhovitskaya, Nina I.; Cowan, Graham H.; Vetukuri, Ramesh R.; Tilsner, Jens; Torrance, Lesley; Savenkov, Eugene I. (2015). "Importin-α-Mediated Nucleolar Localization of Potato Mop-Top Virus TRIPLE GENE BLOCK1 (TGB1) Protein Facilitates Virus Systemic Movement, Whereas TGB1 Self-Interaction is Required for Cell-to-Cell Movement in Nicotiana benthamiana". Plant Physiology. 167 (3): 738–752. doi:10.1104/pp.114.254938. PMC   4348779 . PMID   25576325.
  12. ↑ "Bulletin #2437, Potato Mop-Top Virus (PMTV)". umaine.edu. University of Maine: Cooperative Extension Publications. Retrieved 2015-11-11.
  13. 1 2 3 Davey T (2009). "The Importance of Potato Mop-top Virus (PMTV) in Scottish Seed Potatoes". British Potato Council.
  14. 1 2 3 "Bulletin #2437, Potato Mop-Top Virus (PMTV) | Cooperative Extension Publications | University of Maine". umaine.edu. Retrieved 2015-10-22.
  15. 1 2 Shah FA, Falloon RE, Butler RC, Lister RA, Thomas SM, Curtin D (2014-09-17). "Agronomic factors affect powdery scab of potato and amounts of Spongospora subterranea DNA in soil". Australasian Plant Pathology. 43 (6): 679–689. doi:10.1007/s13313-014-0317-4. hdl: 10182/10430 . ISSN   0815-3191. S2CID   18388456.
  16. 1 2 Beuch U (2013). "Distribution and Diversity of Potato Mop-top Virus in Sweden". Acta Universitatis Agriculturae Sueciae.
  17. ↑ Savenkov EI, Sandgren M, Valkonen JP (October 1999). "Complete sequence of RNA 1 and the presence of tRNA-like structures in all RNAs of Potato mop-top virus, genus Pomovirus". The Journal of General Virology. 80 ( Pt 10) (10): 2779–84. doi: 10.1099/0022-1317-80-10-2779 . PMID   10573175.
  18. ↑ Kashiwazaki S, Scott KP, Reavy B, Harrison BD (January 1995). "Sequence analysis and gene content of potato mop-top virus RNA 3: further evidence of heterogeneity in the genome organization of furoviruses". Virology. 206 (1): 701–6. doi: 10.1016/S0042-6822(95)80092-1 . PMID   7831829.
  19. ↑ Scott KP, Kashiwazaki S, Reavy B, Harrison BD (December 1994). "The nucleotide sequence of potato mop-top virus RNA 2: a novel type of genome organization for a furovirus". The Journal of General Virology. 75 ( Pt 12) (12): 3561–8. doi: 10.1099/0022-1317-75-12-3561 . PMID   7996148.
  20. ↑ Lukhovitskaya NI, Thaduri S, Garushyants SK, Torrance L, Savenkov EI (June 2013). "Deciphering the mechanism of defective interfering RNA (DI RNA) biogenesis reveals that a viral protein and the DI RNA act antagonistically in virus infection". Journal of Virology. 87 (11): 6091–103. doi:10.1128/JVI.03322-12. PMC   3648117 . PMID   23514891.
  21. ↑ Latvala-Kilby S, Aura JM, Pupola N, Hannukkala A, Valkonen JP (May 2009). "Detection of potato mop-top virus in potato tubers and sprouts: combinations of RNA2 and RNA3 variants and incidence of symptomless infections". Phytopathology. 99 (5): 519–31. doi:10.1094/phyto-99-5-0519. PMID   19351248.
  22. ↑ Beuch U, Berlin S, Åkerblom J, Nicolaisen M, Nielsen SL, Crosslin JM, Hamm PB, Santala J, Valkonen JP, Kvarnheden A (May 2015). "Diversity and evolution of potato mop-top virus". Archives of Virology. 160 (5): 1345–51. doi:10.1007/s00705-015-2381-7. PMID   25753427. S2CID   11063371.
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