Formimidoylglutamase

formimidoylglutamase
Identifiers
EC no.	3.5.3.8
CAS no.	9054-92-6
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a formimidoylglutamase (EC 3.5.3.8) is an enzyme that catalyzes the chemical reaction

N-formimidoyl-L-glutamate + H₂O ${\displaystyle \rightleftharpoons }$ L-glutamate + formamide

Thus, the two substrates of this enzyme are N-formimidoyl-L-glutamate and H₂O, whereas its two products are L-glutamate and formamide.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. The systematic name of this enzyme class is N-formimidoyl-L-glutamate formimidoylhydrolase. Other names in common use include formiminoglutamase, N-formiminoglutamate hydrolase, and N-formimino-L-glutamate formiminohydrolase. This enzyme participates in histidine metabolism.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1XFK and 2A0M.

References

• Kaminskas E, Kimhi Y, Magasanik B (1970). "Urocanase and N-formimino-L-glutamate formiminohydrolase of Bacillus subtilis, two enzymes of the histidine degradation pathway". J. Biol. Chem. 245 (14): 3536–44. doi: 10.1016/S0021-9258(18)62959-X . PMID   4990470.
• Lund P, Magasanik B (1965). "N-formimino-L-glutamate formiminohydrolase of Aerobacter aerogenes". J. Biol. Chem. 240 (11): 4316–9. doi: 10.1016/S0021-9258(18)97062-6 . PMID   5845833.