List of restriction enzyme cutting sites: Bst–Bv

Last updated January 20, 2023

Legend of nucleobases
Code	Nucleotide represented
A	Adenine (A)
C	Cytosine (C)
G	Guanine (G)
T	Thymine (T)
N	A, C, G or T
M	A or C
R	A or G
W	A or T
Y	C or T
S	C or G
K	G or T
H	A, C or T
B	C, G or T
V	A, C or G
D	A, G or T

This article contains a list of the most studied restriction enzymes whose names start with Bst to Bv inclusive. It contains approximately 200 enzymes.

Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature ^[1]^[2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action^[3]^[4].
Source: Organism that naturally produces the enzyme.
Recognition sequence : Sequence of DNA recognized by the enzyme and to which it specifically binds.
Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site^[5]^[6].
Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:

as in this not listed enzyme:

EcoR70I

Whole list navigation

Restriction enzymes

Bst

Enzyme PDB code Source Recognition sequence Cut Isoschizomers

BstI Bacillus stearothermophilus 1503-4R 5' GGATCC
3' CCTAGG 5' ---G GATCC--- 3'
3' ---CCTAG G--- 5' AccEBI, AliI, ApaCI, AsiI, BamHI, BnaI, BspAAIII, Nsp29132II

Bst1I Bacillus stearothermophilus 1 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' AglI, BseBI, Bse17I, BstNI, CbrI, CthII, EcoRII, MvaI, SspAI, TaqXI

Bst2I Bacillus stearothermophilus 2 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' AglI, BseBI, Bse17I, BstNI, BstOI, Bst2UI, CthII, EcoRII, MvaI

Bst6I Bacillus stearothermophilus 6 5' CTCTTC
3' GAGAAG 5' ---CTCTTCN NNN--- 3'
3' ---GAGAAGNNNN --- 5'

Bst11I Bacillus stearothermophilus 11 5' ACTGG
3' TGACC 5' ---ACTGGN --- 3'
3' ---TGAC CN--- 5'

Bst12I Bacillus stearothermophilus 12 5' GCAGC
3' CGTCG 5' ---GCAGCN₇N NNNN--- 3'
3' ---CGTCGN₇NNNNN --- 5' AlwXI, BbvI, BseKI, BseXI, Bsp423I, Bst71I, BstV1I

Bst19I Bacillus stearothermophilus 19 5' GCATC
3' CGTAG 5' ---GCATCNNNN NN--- 3'
3' ---CGTAGNNNNNN --- 5'

Bst19II Bacillus stearothermophilus 19 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' BfuCI, Bsp2095I, BspKT6I, CcyI, FnuCI, MboI, NmeCI, SsiAI

Bst28I Bacillus stearothermophilus 28 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' BciBI, BdiI, Bli41I, Bsa29I, Bsp106I, BspDI, LplI, SpmI, ZhoI

Bst38I Bacillus stearothermophilus 38 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' AorI, BseBI, Bse17I, BsiLI, BspNI, BstOI, Bst2UI, EcoRII, MvaI

Bst40I Bacillus stearothermophilus 40 5' CCGG
3' GGCC 5' ---C CGG--- 3'
3' ---GGC C--- 5'

Bst71I Bacillus stearothermophilus 71 5' GCAGC
3' CGTCG 5' ---GCAGCN₇N NNNN--- 3'
3' ---CGTCGN₇NNNNN --- 5' AlwXI, BbvI, BseKI, BseXI, Bsp423I, Bst12I, BstV1I

Bst98I Bacillus stearothermophilus C98 5' CTTAAG
3' GAATTC 5' ---C TTAAG--- 3'
3' ---GAATT C--- 5'

Bst100I Bacillus stearothermophilus 100 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' AorI, BseBI, Bse24I, BstNI, BstOI, Bst2UI, BthDI, EcoRII, MvaI, SleI

Bst1107I Bacillus stearothermophilus RFL1107 5' GTATAC
3' CATATG 5' ---GTA TAC--- 3'
3' ---CAT ATG--- 5'

BstACI Bacillus stearothermophilus AC 5' GRCGYC
3' CYGCRG 5' ---GR CGYC--- 3'
3' ---CYGC RG--- 5' AcyI, AhaII, AosII, AstWI, AsuIII, BsaHI, HgiI, HgiDI, Hsp92I

BstAPI Bacillus stearothermophilus AP 5' GCAN₄NTGC
3' CGTN₄NACG 5' ---GCANNNN NTGC--- 3'
3' ---CGTN NNNNACG--- 5' ApaBI

BstAUI

Bacillus stearothermophilus AU

5' TGTACA
3' ACATGT

5' ---T GTACA--- 3'
3' ---ACATG T--- 5'

AauI,	BsmRI,	Bsp1407I, BsrGI,
Ssp4800I, SspBI

BstBI Bacillus stearothermophilus B225 5' TTCGAA
3' AAGCTT 5' ---TT CGAA--- 3'
3' ---AAGC TT--- 5' AcpI, Bim19I, Bpu14I, BspT104I, Csp45I, LspI, NspV, SspRFI

Bst2BI Bacillus stearothermophilus 2B 5' CACGAG
3' GTGCTC 5' ---C ACGAG--- 3'
3' ---GTGCT C--- 5'

BstBAI Bacillus stearothermophilus BA 5' YACGTR
3' RTGCAY 5' ---YAC GTR--- 3'
3' ---RTG CAY--- 5'

BstBSI Bacillus stearothermophilus BS 5' GTATAC
3' CATATG 5' ---GTA TAC--- 3'
3' ---CAT ATG--- 5'

BstB7SI Bacillus stearothermophilus B7S 5' RCCGGY
3' YGGCCR 5' ---R CCGGY--- 3'
3' ---YGGCC R--- 5'

BstBS32I Bacillus stearothermophilus BS32 5' GAAGAC
3' CTTCTG 5' ---GAAGACNN NNNN--- 3'
3' ---CTTCTGNNNNNN --- 5'

BstBZ153I Bacillus stearothermophilus BZ153 5' GCGCGC
3' CGCGCG 5' ---G CGCGC--- 3'
3' ---CGCGC G--- 5'

Bst4CI Bacillus stearothermophilus 4C 5' ACNGT
3' TGNCA 5' ---ACN GT--- 3'
3' ---TG NCA--- 5'

BstC8I Bacillus stearothermophilus C8 5' GCNNGC
3' CGNNCG 5' ---GCN NGC--- 3'
3' ---CGN NCG--- 5'

BstD102I Bacillus stearothermophilus D102 5' CCGCTC
3' GGCGAG 5' ---CCG CTC--- 3'
3' ---GGC GAG--- 5' AccBSI, BsrBI, Bst31NI, MbiI

BstDEI Bacillus stearothermophilus DE 5' CTNAG
3' GANTC 5' ---C TNAG--- 3'
3' ---GANT C--- 5'

BstDSI Bacillus stearothermophilus DS 5' CCRYGG
3' GGYRCC 5' ---C CRYGG--- 3'
3' ---GGYRC C--- 5'

BstEII Bacillus stearothermophilus ET 5' GGTNACC
3' CCANTGG 5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5' AcrII, BstPI, BstT9I, BstT10I, EcaI, Eco91I, EcoO65I, NspSAII, PspEI

BstENI Bacillus stearothermophilus EN 5' CCTN₄NAGG
3' GGAN₄NTCC 5' ---CCTNN NNNAGG--- 3'
3' ---GGANNN NNTCC--- 5'

BstENII Bacillus stearothermophilus EN 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' BfuCI, Bsp2095I, BspKT6I, CcyI, FnuCI, MboI, NmeCI, SsiAI

BstEZ359I Bacillus stearothermophilus EZ359 5' GTTAAC
3' CAATTG 5' ---GTT AAC--- 3'
3' ---CAA TTG--- 5'

BstFI Bacillus stearothermophilus FH58 5' AAGCTT
3' TTCGAA 5' ---A AGCTT--- 3'
3' ---TTCGA A--- 5'

BstF5I Bacillus stearothermophilus F5 5' GGATG
3' CCTAC 5' ---GGATGNN --- 3'
3' ---CCTAC NN--- 5'

BstFNI Bacillus stearothermophilus 5' CGCG
3' GCGC 5' ---CG CG--- 3'
3' ---GC GC--- 5' AccII, BceBI, BepI, Bpu95I, BstUI, Csp68KVI, FauBII, MvnI, SelI

BstFZ438I Bacillus stearothermophilus FZ438 5' CCCGC
3' GGGCG 5' ---CCCGCNNNN NN--- 3'
3' ---GGGCGNNNNNN --- 5'

BstGZ53I Bacillus stearothermophilus GZ53 5' CGTCTC
3' GCAGAG 5' ---CGTCTCN NNNN--- 3'
3' ---GCAGAGNNNNN --- 5'

BstH2I

Bacillus stearothermophilus H2

5' RGCGCY
3' YCGCGR

5' ---RGCGC Y--- 3'
3' ---Y CGCGR--- 5'

AccB2I,	BfoI,	Bme142I,
Bsp143II, HaeII, LpnI

BstH9I

Bacillus stearothermophilus H9

5' GGATC
3' CCTAG

5' ---GGATCNNNN N--- 3'
3' ---CCTAGNNNNN --- 5'

AclWI, AlwI, BinI,

BpuFI,

BspPI,

Bst31TI, BthII, Bth617I,

EacI

BstHHI Bacillus stearothermophilus HH 5' GCGC
3' CGCG 5' ---GCG C--- 3'
3' ---C GCG--- 5' AspLEI, BspLAI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI, SciNI

BstHPI Bacillus stearothermophilus HP 5' GTTAAC
3' CAATTG 5' ---GTT AAC--- 3'
3' ---CAA TTG--- 5'

BstHZ55I Bacillus stearothermophilus HZ55 5' CCAN₆TGG
3' GGTN₆ACC 5' ---CCANNNNN NTGG--- 3'
3' ---GGTN NNNNNACC--- 5'

BstIZ316I Bacillus stearothermophilus IZ316 5' CACNNNGTG
3' GTGNNNCAC 5' ---CACNNN GTG--- 3'
3' ---GTG NNNCAC--- 5' AdeI, DraIII

BstJZ301I Bacillus stearothermophilus JZ301 5' CTNAG
3' GANTC 5' ---C TNAG--- 3'
3' ---GANT C--- 5'

BstKTI Bacillus stearothermophilus KT 5' GATC
3' CTAG 5' ---GAT C--- 3'
3' ---C TAG--- 5' AspMDI, Bsp2095I, Bst19II, BspKT6I, DpnII, MboI, NmeCI, SsiAI

BstM6I Bacillus stearothermophilus M6 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' AorI, BseBI, Bse24I, BstNI, BstOI, Bst2UI, BthDI, Fsp1604I, MvaI

BstMAI

Bacillus stearothermophilus MA

5' GTCTC
3' CAGAG

5' ---GTCTCN NNNNN--- 3'
3' ---CAGAGNNNNN N--- 5'

Alw26I,	BcoDI, BscQII,	BsmAI,
BsoMAI

BstMBI Bacillus stearothermophilus MB 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' Bce243I, Bsp105I, BspFI, BstKTI, CpfI, HacI, NdeII, Sth368I

BstMCI Bacillus stearothermophilus MC 5' CGRYCG
3' GCYRGC 5' ---CGRY CG--- 3'
3' ---GC YRGC--- 5'

BstMWI Bacillus stearothermophilus MW 5' GCN₇GC
3' CGN₇CG 5' ---GCNNNNN NNGC--- 3'
3' ---CGNN NNNNNCG--- 5'

BstMZ611I Bacillus stearothermophilus MZ611 5' CCNGG
3' GGNCC 5' --- CCNGG--- 3'
3' ---GGNCC --- 5'

BstNI Bacillus stearothermophilus 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' AjnI, BciBII, BptI, Bst1I, BstOI, Bst2UI, Fsp1604I, SniI, Sth117I

Bst31NI Bacillus stearothermophilus 31N 5' CCGCTC
3' GGCGAG 5' ---CCG CTC--- 3'
3' ---GGC GAG--- 5' AccBSI, BsrBI, BstD102I, MbiI

BstNSI Bacillus stearothermophilus 1161NS 5' RCATGY
3' YGTACR 5' ---RCATG Y--- 3'
3' ---Y GTACR--- 5'

BstNZ169I Bacillus stearothermophilus NZ169 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' BanIII, BciBI, BdiI, Bli41I, Bsa29I, BspDI, LplI, Rme21I, SpmI, ZhoI

BstOI Bacillus stearothermophilus O22 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' AjnI, ApyI, BciBII, BptI, Bst1I, Bst2UI, Fsp1604I, SniI, Sth117I

BstOZ616I Bacillus stearothermophilus OZ616 5' GGGAC
3' CCCTG 5' ---GGGACN₈NN NNNN--- 3'
3' ---CCCTGN₈NNNNNN --- 5'

BstPI Bacillus stearothermophilus 5' GGTNACC
3' CCANTGG 5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5' AcrII, AspAI, BstEII, BstT9I, EcaI, Eco91I, EcoO65I, NspSAII, PspEI

BstPAI Bacillus stearothermophilus PA 5' GACN₄GTC
3' CTGN₄CAG 5' ---GACNN NNGTC--- 3'
3' ---CTGNN NNCAG--- 5'

BstPZ418I 5' GGATG
3' CCTAC 5' ---GGATGN₈N NNNN--- 3'
3' ---CCTACN₈NNNNN --- 5'

BstPZ740I Bacillus stearothermophilus PZ740 5' CTTAAG
3' GAATTC 5' ---C TTAAG--- 3'
3' ---GAATT C--- 5'

BstRZ246I Bacillus stearothermophilus RZ246 5' ATTTAAAT
3' TAAATTTA 5' ---ATTT AAAT--- 3'
3' ---TAAA TTTA--- 5'

BstSI Bacillus stearothermophilus S183 5' CYCGRG
3' GRGCYC 5' ---C YCGRG--- 3'
3' ---GRGCY C--- 5' Ama87I, AvaI, Bse15I, BsoBI, Eco27kI, NspIII, OfoI, PunAI

BstSCI Bacillus stearothermophilus SC 5' CCNGG
3' GGNCC 5' --- CCNGG--- 3'
3' ---GGNCC --- 5'

BstSFI Bacillus stearothermophilus SF 5' CTRYAG
3' GAYRTC 5' ---C TRYAG--- 3'
3' ---GAYRT C--- 5'

BstSNI Bacillus stearothermophilus SN 5' TACGTA
3' ATGCAT 5' ---TAC GTA--- 3'
3' ---ATG CAT--- 5'

BstSWI Bacillus stearothermophilus SW 5' ATTTAAAT
3' TAAATTTA 5' ---ATTT AAAT--- 3'
3' ---TAAA TTTA--- 5'

BstT7I

Bacillus stearothermophilus T7

5' TGATCA
3' ACTAGT

5' ---T GATCA--- 3'
3' ---ACTAG T--- 5'

AbaI, BclI, BsiQI,
BspXII, FbaI, Ksp22I,	ParI

BstT9I Bacillus stearothermophilus T9 5' GGTNACC
3' CCANTGG 5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5' AspAI, Bse64I, BstT10I, BstPI, Eco91I, EcoO128I, NspSAII, PspEI

BstT10I Bacillus stearothermophilus T10 5' GGTNACC
3' CCANTGG 5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5' AspAI, BseT10I, BstEII, BstT10I, Eco91I, EcoO128I, NspSAII, PspEI

Bst31TI Bacillus stearothermophilus 31T 5' GGATC
3' CCTAG 5' ---GGATCNNNN N--- 3'
3' ---CCTAGNNNNN --- 5'

BstTS5I Bacillus stearothermophilus TS5 5' GAAGAC
3' CTTCTG 5' ---GAAGACNN NNNN--- 3'
3' ---CTTCTGNNNNNN --- 5'

BstUI Bacillus stearothermophilus U458 5' CGCG
3' GCGC 5' ---CG CG--- 3'
3' ---GC GC--- 5' AccII, BceBI, BepI, Bpu95I, BtkI, Csp68KVI, FauBII, MvnI, SelI

Bst2UI Bacillus stearothermophilus 2U 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' AjnI, BciBII, BptI, Bst1I, BstOI, BstM6I, SniI, SslI, TaqXI, ZanI

BstVI Bacillus stearothermophilus V 5' CTCGAG
3' GAGCTC 5' ---C TCGAG--- 3'
3' ---GAGCT C--- 5' BluI, BssHI, MlaAI, PanI, SauLPII, SciI, SlaI, StrI, XhoI

BstV1I Bacillus stearothermophilus V1 5' GCAGC
3' CGTCG 5' ---GCAGCN₇N NNNN--- 3'
3' ---CGTCGN₇NNNNN --- 5' AlwXI, BbvI, BseKI, BseXI, Bsp423I, Bst12I, Bst71I

BstV2I Bacillus stearothermophilus V2 5' GAAGAC
3' CTTCTG 5' ---GAAGACNN NNNN--- 3'
3' ---CTTCTGNNNNNN --- 5'

BstXI Bacillus stearothermophilus X1 5' CCAN₆TGG
3' GGTN₆ACC 5' ---CCANNNNN NTGG--- 3'
3' ---GGTN NNNNNACC--- 5'

BstX2I Bacillus stearothermophilus X2 5' RGATCY
3' YCTAGR 5' ---R GATCY--- 3'
3' ---YCTAG R--- 5'

BstYI 1SDO Bacillus stearothermophilus Y406 5' RGATCY
3' YCTAGR 5' ---R GATCY--- 3'
3' ---YCTAG R--- 5'

BstZI Bacillus stearothermophilus Z130 5' CGGCCG
3' GCCGGC 5' ---C GGCCG--- 3'
3' ---GCCGG C--- 5' AaaI, BseX3I, EagI, EclXI, Eco52I, SenPT16I, XmaIII

BstZ17I Bacillus stearothermophilus 38M 5' GTATAC
3' CATATG 5' ---GTA TAC--- 3'
3' ---CAT ATG--- 5'

Bsu - Bv

Enzyme	PDB code	Source	Recognition sequence	Cut	Isoschizomers
Bsu6I		Bacillus subtilis 6v1	`5' CTCTTC` `3' GAGAAG`	`5' ---CTCTTCN NNN--- 3'` `3' ---GAGAAGNNNN --- 5'`
Bsu15I		Bacillus subtilis 15	`5' ATCGAT` `3' TAGCTA`	`5' ---AT CGAT--- 3'` `3' ---TAGC TA--- 5'`	AagI, BanIII, BavCI, Bsa29I, BseCI, BspDI, BsuTUI, ClaI
Bsu23I		Bacillus subtilis 23	`5' TCCGGA` `3' AGGCCT`	`5' ---T CCGGA--- 3'` `3' ---AGGCC T--- 5'`	AccIII, BbvAIII, BseAI, BsiMI, BspEI, BspMII, CauB3I, MroI
Bsu36I		Bacillus subtilis 36	`5' CCTNAGG` `3' GGANTCC`	`5' ---CC TNAGG--- 3'` `3' ---GGANT CC--- 5'`	AxyI, BliHKI, BspR7I, Bsu36I, Eco81I, MstII, OxaNI, SshAI
Bsu54I		Bacillus subtilis 54	`5' GGNCC` `3' CCNGG`	`5' ---G GNCC--- 3'` `3' ---CCNG G--- 5'`	AsuI, Bac36I, BavBII, BsiZI, BspBII, CcuI, MaeK81II, PspPI
Bsu1532I		Bacillus subtilis 1532	`5' CGCG` `3' GCGC`	`5' ---CG CG--- 3'` `3' ---GC GC--- 5'`	BceBI, Bpu95I, Bsp123I, BstUI, BtkI, Csp68KVI, FnuDII, MvnI, ThaI
Bsu1854I		Bacillus subtilis 1854	`5' GRGCYC` `3' CYCGRG`	`5' ---GRGCY C--- 3'` `3' ---C YCGRG--- 5'`
BsuBI		Bacillus subtilis	`5' CTGCAG` `3' GACGTC`	`5' ---CTGCA G--- 3'` `3' ---G ACGTC--- 5'`	AjoI, BloHII, CflI, CstI, Ecl37kI, HalII, Psp23I, PstI, SalPI, SflI, YenI
BsuFI		Bacillus subtilis	`5' CCGG` `3' GGCC`	`5' ---C CGG--- 3'` `3' ---GGC C--- 5'`
BsuMI		Bacillus subtilis 168	`5' CTCGAG` `3' GAGCTC`	`5' ---C TCGAG--- 3'` `3' ---GAGCT C--- 5'`	XhoI, BssHI, TliI, PaeR7I, Sfr274I, SlaI, StrI
BsuRI		Bacillus subtilis R	`5' GGCC` `3' CCGG`	`5' ---GG CC--- 3'` `3' ---CC GG--- 5'`	HaeIII
BsuTUI		Bacillus subtilis TU	`5' ATCGAT` `3' TAGCTA`	`5' ---AT CGAT--- 3'` `3' ---TAGC TA--- 5'`	AagI, BanIII, BavCI, Bsa29I, BseCI, BspDI, Bsu15I, ClaI
BteI		Brochothrix thermosphacter	`5' GGCC` `3' CCGG`	`5' ---GG CC--- 3'` `3' ---CC GG--- 5'`
BtgI		Bacillus thermoglucosidasius	`5' CCRYGG` `3' GGYRCC`	`5' ---C CRYGG--- 3'` `3' ---GGYRC C--- 5'`
BtgZI		Bacillus thermoglucosidasius	`5' GCGATG` `3' CGCTAC`	`5' ---GCGATGN₈NN NNNN--- 3'` `3' ---CGCTACN₈NNNNNN --- 5'`
BthAI		Bacillus thuringiensis AII	`5' GGWCC` `3' CCWGG`	`5' ---G GWCC--- 3'` `3' ---CCWG G--- 5'`	BamNxI, BcuAI, BsrAI, Eco47I, FspMSI, HgiCII, HgiJI, SmuEI
BthCI		Bacillus thuringiensis 2294	`5' GCNGC` `3' CGNCG`	`5' ---GCNG C--- 3'` `3' ---C GNCG--- 5'`
BthDI		Bacillus thuringiensis D4	`5' CCWGG` `3' GGWCC`	`5' ---CC WGG--- 3'` `3' ---GGW CC--- 5'`	AorI, ApyI, Bse24I, BstNI, BstOI, Bst2UI, CbrI, EcoRII, MvaI, SleI
BthEI		Bacillus thuringiensis E	`5' CCWGG` `3' GGWCC`	`5' ---CC WGG--- 3'` `3' ---GGW CC--- 5'`	AorI, BseBI, Bse24I, BstNI, BstOI, Bst2UI, BthDI, EcoRII, MvaI, SleI
BtkI		Bacillus thuringiensis Kumantoensis	`5' CGCG` `3' GCGC`	`5' ---CG CG--- 3'` `3' ---GC GC--- 5'`	BepI, Bpu95I, Bsp123I, BstFNI, BstUI, BtkI, FalII, SelI, ThaI
BtkII		Bacillus thuringiensis Kumantoensis	`5' GATC` `3' CTAG`	`5' --- GATC--- 3'` `3' ---CTAG --- 5'`	BfuCI, Bsp67I, Bst19II, CcyI, FnuCI, MboI, NmeCI, SauMI
BtrI		Bacillus stearothermophilus SE-U62	`5' CACGTC` `3' GTGCAG`	`5' ---CAC GTC--- 3'` `3' ---GTG CAG--- 5'`
BtsI		Bacillus thermoglucosidasius	`5' GCAGTG` `3' CGTCAC`	`5' ---GCAGTGNN --- 3'` `3' ---CGTCAC NN--- 5'`
BveI		Brevundimonas vesicularis RFL1	`5' ACCTGC` `3' TGGACG`	`5' ---ACCTGCNNNN NNNN--- 3'` `3' ---TGGACGNNNNNNNN --- 5'`	Acc36I, BfuAI, BspMI
BvuI		Bacillus vulgatis	`5' GRGCYC` `3' CYCGRG`	`5' ---GRGCY C--- 3'` `3' ---C YCGRG--- 5'`
BvuBI		Bacteroides vulgatus	`5' CGTACG` `3' GCATGC`	`5' ---C GTACG--- 3'` `3' ---GCATG C--- 5'`

Notes

↑ Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.
↑ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. doi:10.1093/nar/gkg274. PMC 152790 . PMID 12654995.
↑ Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN 0-7167-4684-0.
↑ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID 4124164.
↑ Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene. 92 (1–2): 1–248. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.
↑ Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. doi:10.1093/nar/29.18.3705. PMC 55916 . PMID 11557805.

v t e Restriction modification system: restriction enzyme
Basic Concept	Isoschizomer Neoschizomer Isocaudomer
Recognition sequence 4bp	TaqI Sau3A AluI* HaeIII* DpnII NlaIII HpaII
Recognition sequence 5bp	EcoRII FokI
Recognition sequence 6bp	AaaI BglII PovII* EcoRV* EcoRI BamHI HindIII XbaI XhoI SacI PstI NdeI
Recognition sequence 8bp	NotI
Lists	A Ba–Bc Bd–Bp Bsa–Bso Bsp–Bss Bst–Bv C-D E-F G-K L-N O-R S T-Z
* means cleavage produces blunt ends

List of restriction enzyme cutting sites: Bst–Bv

Contents

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Restriction enzymes

Bst

Bsu - Bv

Notes

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