Methylenediurea deaminase

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Methylenediurea deaminase
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EC no. 3.5.3.21
CAS no. 205830-62-2
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Methylenediurea deaminase (EC 3.5.3.21, methylenediurease) is an enzyme with systematic name methylenediurea aminohydrolase found in Brucella anthropi , a bacterium. [1] This enzyme catalyses the following chemical reaction:

methylenediurea + 2 H2O N-(hydroxymethyl)urea + 2 NH3 + CO2 (overall reaction)
(1a) methylenediurea + H2O N-(carboxyaminomethyl)urea + NH3
(1b) N-(carboxyaminomethyl)urea N-(aminomethyl)urea + CO2 (spontaneous)
(1c) N-(aminomethyl)urea + H2O N-(hydroxymethyl)urea + NH3 (spontaneous)

Methylenediurea is hydrolysed and decarboxylated to give an aminated methylurea.

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References

  1. ↑ Jahns T, Schepp R, Kaltwasser H (1997). "Purification and characterisation of an enzyme from a strain of Ochrobactrum anthroπ that degrades condensation products of urea and formaldehyde (ureaform)". Canadian Journal of Microbiology. 43: 1111–1117. doi:10.1139/m97-159.
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