Methylobacterium

Last updated

Methylobacterium
Methylobacterium jeotgali.jpg
Methylobacterium jeotgali strain S2R03-9T
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Bacteria
Phylum: Pseudomonadota
Class: Alphaproteobacteria
Order: Hyphomicrobiales
Family: Methylobacteriaceae
Genus: Methylobacterium
Patt et al. 1976 (Approved Lists 1980)
Type species
Methylobacterium organophilum [1]
Species

See text.

Methylobacterium is a genus of bacteria, also designated as Pink-Pigmented Facultative Methylotrophs (PPFMs). Methylobacterium is commonly found in soil, water, and particularly in association with the phyllosphere, the aerial part of plants. [2] [3] Methylobacterium is characterized by its ability to metabolize one-carbon compounds like methanol released by plant leaves. [4] Methylobacterium plays key roles in nutrient cycling and plant-microbe interactions, often promoting plant growth and resilience through hormone production and stress mitigation. [5] [6] Methylobacterium is a model in biotechnology, already used commercially for protein production, bioremediation, and as a biostimulant in agriculture. [7] [8]

Contents

Methylobacterium has also been identified as a contaminant of DNA extraction kit reagents, which may lead to its erroneous appearance in microbiota or metagenomic datasets. [9] In March 2021, a new species, named Methylobacterium ajmalii , [10] associated with three new strains, designated IF7SW-B2T, IIF1SW-B5, and IIF4SW-B5, were reported to have been discovered, for the first time, on the International Space Station. [11] [12]

Consensus phylogeny of Methylobacterium species built from 213 Methylobacteriaceae genomes (adapted from Leducq et al. 2022 ) Astral-species-tree-GBEpaper-ForWiki.jpg
Consensus phylogeny of Methylobacterium species built from 213 Methylobacteriaceae genomes (adapted from Leducq et al. 2022 )

Debate on Methylobacterium taxonomy

In 2018, 11 Methylobacterium species, including Methylobacterium extorquens , were transfered to Methylorubrum . [14] This emendation mostly relied on the 16S rRNA gene phylogeny. In 2021 and 2022, two studies based on phylogenomic and phenotypic data independently pointed the lack of support for Methylorubrum as a distinct genus, calling for its abandonment and the reinstatement of the former Methylobacterium taxonomy. [15] [16] In 2025, despite controversy, and its emendation back to the original Methylobacterium taxonomy, [16] Methylorubrum was still a valid genus name in some databases like NCBI and in the List of Prokaryotic names with Standing in Nomenclature. [17]

Natural genetic transformation

Natural genetic transformation in bacteria is a process involving transfer of DNA from one cell to another through the intervening medium, and the integration of the donor sequence into the recipient genome by homologous recombination. Methylobacterium organophilum cells are able to undergo genetic transformation and become competent for DNA uptake near the end of the exponential growth phase. [18]

Species

Methylobacterium comprises the following species [19] (excluding species reclassified in Methylorubrum [14] ):

References

  1. ↑ Parte, A.C. "Methylobacterium". LPSN .
  2. ↑ Zhang, Cong; Wang, Meng-Ying; Khan, Naeem; Tan, Ling-Ling; Yang, Song (January 2021). "Potentials, Utilization, and Bioengineering of Plant Growth-Promoting Methylobacterium for Sustainable Agriculture". Sustainability. 13 (7): 3941. doi: 10.3390/su13073941 .
  3. ↑ Leducq JB, Seyer Lamontagne E, Condrain Morel D, Bourret G, Sneddon D, Foster JA, Marx CJ, Sullivan JM, Shapiro BJ, Kembel SW (2021). "Fine-Scale Adaptations to Environmental Variation and Growth Strategies Drive Phyllosphere Methylobacterium Diversity". mBio. doi: 10.1128/mbio.03175-21 . PMC   8787475 . PMID   35073752.
  4. ↑ Tani, Akio; Masuda, Sachiko; Fujitani, Yoshiko; Iga, Toshiki; Haruna, Yuuki; Kikuchi, Shiho; Shuaile, Wang; Lv, Haoxin; Katayama, Shiori; Yurimoto, Hiroya; Sakai, Yasuyoshi; Kato, Junichi (13 October 2023). "Metabolism-linked methylotaxis sensors responsible for plant colonization in Methylobacterium aquaticum strain 22A". Frontiers in Microbiology. 14. doi: 10.3389/fmicb.2023.1258452 . PMC   10613068 . PMID   37901831.
  5. ↑ Vogel, Christine; Bodenhausen, Natacha; Gruissem, Wilhelm; Vorholt, Julia A. (2016). "The Arabidopsis leaf transcriptome reveals distinct but also overlapping responses to colonization by phyllosphere commensals and pathogen infection with impact on plant health" (PDF). New Phytologist. 212 (1): 192–207. doi:10.1111/nph.14036. PMID   27306148.
  6. ↑ Omer, Zahra S.; Tombolini, Riccardo; Gerhardson, Berndt (1 March 2004). "Plant colonization by pink-pigmented facultative methylotrophic bacteria (PPFMs)". FEMS Microbiology Ecology. 47 (3): 319–326. doi:10.1016/S0168-6496(04)00003-0. PMID   19712320.
  7. ↑ Govindaraju, Alekhya; Good, Nathan M; Zytnick, Alexa M; Martinez-Gomez, Norma Cecilia (1 June 2022). "Employing methylotrophs for a green economy: one-carbon to fuel them all and through metabolism redesign them". Current Opinion in Microbiology. 67: 102145. doi:10.1016/j.mib.2022.102145.
  8. ↑ Zhang, Cong; Wang, Meng-Ying; Khan, Naeem; Tan, Ling-Ling; Yang, Song (January 2021). "Potentials, Utilization, and Bioengineering of Plant Growth-Promoting Methylobacterium for Sustainable Agriculture". Sustainability. 13 (7): 3941. doi: 10.3390/su13073941 .
  9. ↑ Salter, S; Cox, M; Turek, E; Calus, S; Cookson, W; Moffatt, M; Turner, P; Parkhill, J; Loman, N; Walker, A (2014). "Reagent contamination can critically impact sequence-based microbiome analyses". bioRxiv   10.1101/007187 .
  10. 1 2 LPSN: Species Methylobacterium ajmalii Bijlani et al. 2021
  11. ↑ Bowler, Jacinta (16 March 2021). "Microbes Unknown to Science Discovered on The International Space Station". ScienceAlert . Retrieved 16 March 2021.
  12. ↑ Rogers, Adam (April 5, 2021). "Sneaky New Bacteria on the ISS Could Build a Future on Mars". Wired . One species, found on a HEPA filter in the station's life-support system, was a garden-variety (literally!) Methylobacterium rhodesianum. But three samples—from a surface near the materials research rack, a wall near the "cupola" of windows, and the astronauts' dining table—were something new.
  13. ↑ Leducq, Jean-Baptiste; Sneddon, David; Santos, Malia; Condrain-Morel, Domitille; Bourret, Geneviève; Martinez-Gomez, N Cecilia; Lee, Jessica A; Foster, James A; Stolyar, Sergey; Shapiro, B Jesse; Kembel, Steven W; Sullivan, Jack M; Marx, Christopher J (1 August 2022). "Comprehensive Phylogenomics of Methylobacterium Reveals Four Evolutionary Distinct Groups and Underappreciated Phyllosphere Diversity". Genome Biology and Evolution. 14 (8): evac123. doi:10.1093/gbe/evac123. PMC   9364378 . PMID   35906926.
  14. 1 2 Green PN, Ardley JK (2018). "Review of the genus Methylobacterium and closely related organisms: A proposal that some Methylobacterium species be reclassified into a new genus, Methylorubrum gen. nov". Int J Syst Evol Microbiol. 68 (9): 2727–2748. doi: 10.1099/ijsem.0.002856 . PMID   30024371.
  15. ↑ Leducq, Jean-Baptiste; Sneddon, David; Santos, Malia; Condrain-Morel, Domitille; Bourret, Geneviève; Martinez-Gomez, N Cecilia; Lee, Jessica A; Foster, James A; Stolyar, Sergey; Shapiro, B Jesse; Kembel, Steven W; Sullivan, Jack M; Marx, Christopher J (1 August 2022). "Comprehensive Phylogenomics of Methylobacterium Reveals Four Evolutionary Distinct Groups and Underappreciated Phyllosphere Diversity". Genome Biology and Evolution. 14 (8): evac123. doi:10.1093/gbe/evac123. PMC   9364378 . PMID   35906926.
  16. 1 2 Alessa O, Ogura Y, Fujitani Y, Takami H, Hayashi T, Sahin N, Tani A (2021). "Comprehensive Comparative Genomics and Phenotyping of Methylobacterium Species". Front Microbiol. 12. doi: 10.3389/fmicb.2021.740610 . PMC   8561711 . PMID   34737731.
  17. ↑ Alleman AB, Stolyar S, Marx CJ, Leducq JB (2025). "Led astray by 16S rRNA: phylogenomics reaffirms the monophyly of Methylobacterium and lack of support for Methylorubrum as a genus". ISME J. doi: 10.1093/ismejo/wraf011 . PMID   39834026.
  18. ↑ O'Connor M, Wopat A, Hanson RS (1977). "Genetic transformation in Methylobacterium organophilum". J. Gen. Microbiol. 98 (1): 265–72. doi: 10.1099/00221287-98-1-265 . PMID   401866.
  19. ↑ Euzéby JP, Parte AC. "Methylobacteriaceae". List of Prokaryotic names with Standing in Nomenclature (LPSN). Retrieved May 22, 2021.
  20. ↑ Bijlani S, Singh NK, Eedara VV, Podile AR, Mason CE, Wang CC, Venkateswaran K (2021). "Methylobacterium ajmalii sp. nov., Isolated From the International Space Station". Front Microbiol. 12: 639396. doi: 10.3389/fmicb.2021.639396 . PMC   8005752 . PMID   33790880.


This page is based on this Wikipedia article
Text is available under the CC BY-SA 4.0 license; additional terms may apply.
Images, videos and audio are available under their respective licenses.