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Names | |
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Preferred IUPAC name 1-Ethyl-2-[(1E,3Z)-3-(1-ethylnaphtho[1,2-d][1,3]thiazol-2(1H)-ylidene)-2-methylprop-1-en-1-yl]naphtho[1,2-d][1,3]thiazol-1-ium bromide | |
Other names DBTC; Carbocyanin DBTC | |
Identifiers | |
3D model (JSmol) | |
ChEBI | |
ChemSpider | |
ECHA InfoCard | 100.028.225 |
EC Number |
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PubChem CID | |
UNII | |
CompTox Dashboard (EPA) | |
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Properties | |
C30H27BrN2S2 | |
Molar mass | 559.58 g·mol−1 |
Hazards | |
GHS labelling: | |
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Warning | |
H315, H319, H335 | |
P261, P264, P264+P265, P271, P280, P302+P352, P304+P340, P305+P351+P338, P319, P321, P332+P317, P337+P317, P362+P364, P403+P233, P405, P501 | |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). |
Stains-all is a carbocyanine dye, which stains anionic proteins, nucleic acids, anionic polysaccharides and other anionic molecules. [1] [2]
Stains-all is metachromatic and changes its color dependent on its contact to other molecules. [3] The detection limit for phosphoproteins is below 1 ng after one hour of staining, [4] for anionic polysaccharides between 10 and 500 ng. [5] [6] Highly anionic proteins are stained blue, proteoglycans purple and anionic proteins pink. [7] RNA is stained blueish-purple with a detection limit of 90 ng and DNA is stained blue with a detection limit of 3 ng. [8]
Stains-all is light sensitive, therefore the staining is performed in the absence of light and photographed immediately. Staining of proteins can be improved by a subsequent silver stain. [7] The analogue Ethyl-Stains-all has similar properties as stains-all, with differences in solubility and staining properties. [9]
Stains-all stains nucleic acids, anionic proteins, anionic polysaccharides such as alginate and pectinate, [10] hyaluronic acid and dermatan sulfate, [5] heparin, heparan sulfate and chondroitin sulfate. [6] It is used in SDS-PAGE, agarose gel electrophoresis and histologic staining, e.g. staining of growth lines in bones. [11]