Chondroitin sulfate

Last updated
Chemical structure of one unit in a chondroitin sulfate chain. Chondroitin-4-sulfate: R1 = H; R2 = SO3H; R3 = H. Chondroitin-6-sulfate: R1 = SO3H; R2, R3 = H. Chondroitin Sulfate Structure NTP.png
Chemical structure of one unit in a chondroitin sulfate chain. Chondroitin-4-sulfate: R1 = H; R2 = SO3H; R3 = H. Chondroitin-6-sulfate: R1 = SO3H; R2, R3 = H.

Chondroitin sulfate is a sulfated glycosaminoglycan (GAG) [1] composed of a chain of alternating sugars (N-acetylgalactosamine and glucuronic acid). It is usually found attached to proteins as part of a proteoglycan. [1] A chondroitin chain can have over 100 individual sugars, each of which can be sulfated in variable positions and quantities. Chondroitin sulfate is an important structural component of cartilage, [2] and provides much of its resistance to compression. [3] Along with glucosamine, chondroitin sulfate has become a widely used dietary supplement for treatment of osteoarthritis, although large clinical trials failed to demonstrate any symptomatic benefit of chondroitin.

Contents

Medical use

Chondroitin is used in dietary supplements as an alternative medicine to treat osteoarthritis. [4] It is also approved and regulated as a symptomatic slow-acting drug for this disease (SYSADOA) in Europe and some other countries. It is commonly sold together with glucosamine. [5] A 2015 Cochrane review of clinical trials found that most were of low quality, but that there was some evidence of short-term improvement in pain and few side effects; it does not appear to improve or maintain the health of affected joints. [5]

Chondroitin, along with commonly used glucosamine, should not be used to treat people who have symptomatic osteoarthritis of the knee as evidence shows that these treatments fail to provide relief for that condition. [6]

Chondroitin has shown to be promising in the treatment of coronary artery disease. In a 6-year double-blind placebo-controlled study involving 60 test subjects published in 1973, the chondroitin sulfate group showed a 350% reduction in fatal heart attacks compared to the control group. When analyzing the data for non-fatal cardiovascular events, the control group experienced non-fatal heart attacks at a rate of 16%, while those in the chondroitin sulfate-treated group came in at 0%. [7]

Chondroitin sulfate is used as a dietary supplement for diabetes management. [8] It exhibits anti-inflammatory properties, making it a potential alternative to cortisol for treating enteritis. [9] [10]

Adverse effects

Clinical studies have not identified any significant side effects or overdoses of chondroitin sulfate, which suggest its long-term safety. [11] In 2003 the Task Force of the European League Against Rheumatism (EULAR) committee ranked the level of toxicity of chondroitin sulfate 6 in a 0–100 scale. [12]

Chondroitin sulfate is not metabolized by cytochrome P450. [13]

Pharmacology

Mechanisms of action

The effect of chondroitin sulfate in people with osteoarthritis is likely the result of a number of reactions including its anti-inflammatory activity, the stimulation of the synthesis of proteoglycans and hyaluronic acid, and the decrease in catabolic activity of chondrocytes, inhibiting the synthesis of proteolytic enzymes, nitric oxide, and other substances that contribute to damage the cartilage matrix and cause death of articular chondrocytes. A recent review summarizes data from relevant reports describing the biochemical basis of the effect of chondroitin sulfate on osteoarthritis articular tissues. [14]

Bioavailability and pharmacokinetics

Pharmacokinetic studies performed on humans and experimental animals after oral administration of chondroitin sulfate revealed that it can be absorbed orally. Chondroitin sulfate shows first-order kinetics up to single doses of 3,000 mg. [15] [16] [17] [18] Multiple doses of 800 mg in people with osteoarthritis do not alter the kinetics of chondroitin sulfate. The bioavailability of chondroitin sulfate ranges from 15% to 24% of the orally administered dose. More particularly, on the articular tissue, Ronca et al. [19] reported that chondroitin sulfate is not rapidly absorbed in the gastro-intestinal tract and a high content of labeled chondroitin sulfate is found in the synovial fluid and cartilage.

Physical and chemical properties

Chondroitin sulfate chains are unbranched polysaccharides of variable length containing two alternating monosaccharides: D-glucuronic acid (GlcA) and N-acetyl-D-galactosamine (GalNAc). Some of these GlcA residues may be epimerized into L-iduronic acid (IdoA) at which point the resulting glycosaminoglycan is then referred to as dermatan sulfate, previously referred to as chondroitin sulfate B.

Chondroitin sulfate is sourced from natural products, with high variability in terms of chain length and sulfation pattern. The variability in chondroitin sulfate composition extends to its origin, making it possible to differentiate between chondroitin sulfate from terrestrial and marine sources. One way to look at this difference is in terms of the proportion of disaccharide units: chondroitin sulfate from terrestrial animals is almost exclusively composed of non-sulfated (O) and monosulfated (A and C) units, while in marine species the proportion of disulfated (D, E and B) units is higher. Furthermore, marine chondroitin sulfate chains tend to be longer, with molecular weight of up to 70 kDa in chondroitin sulfate from shark, while in terrestrial animals molecular weight is typically below 45 kDa. [20] [21]

Chondroitin sulfate chains are linked to hydroxyl groups on serine residues of certain proteins. Exactly how proteins are selected for attachment of glycosaminoglycans is not understood. Glycosylated serines are often followed by a glycine and have neighboring acidic residues, but this motif does not always predict glycosylation.

Attachment of the GAG chain begins with four monosaccharides in a fixed pattern: Xyl – Gal – Gal – GlcA. Each sugar is attached by a specific enzyme, allowing for multiple levels of control over GAG synthesis. Xylose begins to be attached to proteins in the endoplasmic reticulum, while the rest of the sugars are attached in the Golgi apparatus. [22]

Chondroitin sulfate is highly soluble in water. [23]

History

Chondroitin sulfate was originally isolated well before the structure was characterised, leading to changes in terminology with time. [24] Early researchers identified different fractions of the substance with letters.

Letter identificationSite of sulfationSystematic name
Chondroitin sulfate Acarbon 4 of the N-acetylgalactosamine (GalNAc) sugarchondroitin-4-sulfate
Chondroitin sulfate Ccarbon 6 of the GalNAc sugarchondroitin-6-sulfate
Chondroitin sulfate Dcarbon 2 of the glucuronic acid and 6 of the GalNAc sugarchondroitin-2,6-sulfate
Chondroitin sulfate Ecarbons 4 and 6 of the GalNAc sugarchondroitin-4,6-sulfate

"Chondroitin sulfate B" is an old name for dermatan sulfate, and it is no longer classified as a form of chondroitin sulfate. [25]

Chondroitin, without the "sulfate", has been used to describe a fraction with little or no sulfation. [26] However, this distinction is not used by all.

Although the name "chondroitin sulfate" suggests a salt with a sulfate counter-anion, this is not the case, as sulfate is covalently bonded to the sugar. Rather, since the molecule has multiple negative charges at physiological pH, a cation is present in salts of chondroitin sulfate. Commercial preparations of chondroitin sulfate typically are the sodium salt. Barnhill et al. have suggested that all such preparations of chondroitin sulfate be referred to as "sodium chondroitin" regardless of their sulfation status. [27]

In 2008 the U.S. Food and Drug Administration (FDA) identified "oversulfated chondroitin sulfate" as a contaminant in heparin originating from China. [28] [29] [30]

Clinical trials for osteoarthritis

In 2004, a petition was submitted to the FDA that a dietary supplement of chondroitin sulfate be labeled as reducing the risk of osteoarthritis, cartilage deterioration, and osteoarthritis-related joint pain, tenderness, and swelling. The FDA denied the request, stating that experiments conducted by the company did not sufficiently demonstrate the effectiveness of the claim. Among other comments, the FDA noted the poor experimental design of some trials. [31]

In 2007, Reichenbach et al. used explicit methods to conduct and report a systematic review of 20 trials and concluded "large-scale, methodologically sound trials indicate that the symptomatic benefit of chondroitin is minimal or nonexistent. Use of chondroitin in routine clinical practice should therefore be discouraged." In contrast, and also in 2007, Bruyere et al. concluded that "there is compelling evidence that glucosamine sulfate and chondroitin sulfate may interfere with progression of OA."

As of 2015 the largest trial conducted with the product was the Glucosamine and Chondroitin Arthritis Intervention Trial (GAIT), a double-blind, randomized, multicenter clinical trial sponsored by the US National Institutes of Health in 1583 people with knee osteoarthritis, which was published in the New England Journal of Medicine in 2006. [5] [32] Subjects were randomly assigned to one of five orally administered treatments: two 250 mg capsules of glucosamine hydrochloride three times daily, two 200 mg capsules of chondroitin sulfate three times daily, two capsules of 250 mg of glucosamine hydrochloride plus 200 mg of chondroitin sulfate three times daily, 200 mg of celecoxib daily, or placebo. Treatment was administered for 24 weeks. It showed no difference from placebo. [5]

Sawitzke A, et al. 2010 evaluated the efficacy and safety of glucosamine and chondroitin sulfate, alone or in combination, as well as celecoxib and placebo on painful knee osteoarthritis over 2 years as a continuation of the GAIT trial. This was a 24-month, double-blind, placebo-controlled study, enrolling 662 people with knee osteoarthritis who satisfied radiographic criteria (Kellgren/Lawrence grade 2 or 3 changes and baseline joint space width of at least 2 mm). This subset continued to receive their randomized treatment (glucosamine 500 mg three times daily, chondroitin sulfate 400 mg three times daily, the combination of glucosamine and chondroitin sulfate, celecoxib 200 mg daily, or placebo) over 24 months. The primary outcome was a 20% reduction in pain over 24 months as measured by the Western Ontario and McMaster University Osteoarthritis Index (WOMAC). Secondary outcomes included an Outcome Measures in Rheumatology/Osteoarthritis Research Society International response and change from baseline in WOMAC pain and function. [5] [33] Over 2 years, none of the treatments (not even the positive control celecoxib) achieved a clinically important difference in WOMAC pain or function as compared with placebo. Adverse reactions were similar among treatment groups and serious adverse events were rare for all treatments. [33]

Research

A 2021 study showed a remarkable (about 40%) reduction of the acute myocardial infarction risk in current chondroitin sulfate users in the high risk cardiovascular subgroups. [34]

Society and culture

Manufacture

Most chondroitin appears to be made from extracts of cartilaginous cow and pig tissues (cow trachea and pig ear and nose), but other sources such as shark, fish, and bird cartilage are also used. Since chondroitin is not a uniform substance, and is naturally present in a wide variety of forms, the precise composition of each supplement will vary. [27] In fact, although many food supplement companies produce their products in compliance with human food processing Good Manufacturing Practice (GMP), most of them do not produce their products in compliance with the GMP regulations for pharmaceuticals, resulting in products not meeting pharmaceutical requirements. [35]

While it is a prescription or over-the-counter drug in 22 countries, chondroitin is regulated in the U.S. as a dietary supplement [36] by the Food and Drug Administration. In Europe, chondroitin sulfate formulations are approved as drugs with evidenced efficacy and safety demonstrated by clinical trials in people with osteoarthritis. [37] Adebowale et al. reported in 2000 that of 32 chondroitin supplements they analysed, only 5 were labeled correctly, and more than half contained less than 40% of the labeled amount. [38] With the introduction of GMP regulations for dietary supplements in 2008, chondroitin sulfate preparations are subject in the US to mandatory labeling standards as well as testing requirements for identity, purity, strength, and composition.[ citation needed ] United States Pharmacopoeia (USP) testing standards for the identification and quantification of chondroitin are well-established.[ citation needed ]

There are no FDA regulations on chondroitin sulfate as a food additive, as it is recognized by the FDA as a component of food and is "generally recognized as safe". [31] However, a proposed application of chondroitin sulfate dietary supplement as a means of preventing joint degeneration was highly scrutinized by the FDA, who stated:

" For conventional foods, this evaluation involves considering whether the ingredient that is the source of the substance is generally recognized as safe (GRAS), approved as a food additive, or authorized by a prior sanction issued by FDA (see 21 CFR 101.70(f)). Dietary ingredients in dietary supplements, however, are not subject to the food additive provisions of the act (see section 201(s)(6) of the Act (21 U.S.C. § 321(s)(6)). Rather, they are subject to the adulteration provisions in section 402 of the Act (21 U.S.C. 342) and, if applicable, the new dietary ingredient provisions in section 413 of the Act (21 U.S.C. 350b), which pertain to dietary ingredients that were not marketed in the United States before October 15, 1994."

— Letter Regarding the Relationship Between the Consumption of Glucosamine and/or Chondroitin Sulfate and a Reduced Risk of: Osteoarthritis; Osteoarthritis-related Joint Pain, Joint Tenderness, and Joint Swelling; Joint Degeneration; and Cartilage Deterioration

In the same letter, the FDA found that studies performed on the dietary supplement form of chondroitin sulfate were insufficient to substantiate claims that it is efficacious in the prevention of joint deterioration, and denied the request to be allowed to label the supplement as such. They further denied the request to market it as safe, given that no human clinical trials were done, citing that animal studies are not sufficient for the approval of a dietary supplement. [31]

Veterinary use

Chondroitin and glucosamine are also used in veterinary medicine for osteoarthritis. [39] [40] [41]

See also

Related Research Articles

<span class="mw-page-title-main">Osteoarthritis</span> Form of arthritis caused by degeneration of joints

Osteoarthritis (OA) is a type of degenerative joint disease that results from breakdown of joint cartilage and underlying bone. It is believed to be the fourth leading cause of disability in the world, affecting 1 in 7 adults in the United States alone. The most common symptoms are joint pain and stiffness. Usually the symptoms progress slowly over years. Other symptoms may include joint swelling, decreased range of motion, and, when the back is affected, weakness or numbness of the arms and legs. The most commonly involved joints are the two near the ends of the fingers and the joint at the base of the thumbs, the knee and hip joints, and the joints of the neck and lower back. The symptoms can interfere with work and normal daily activities. Unlike some other types of arthritis, only the joints, not internal organs, are affected.

<span class="mw-page-title-main">Celecoxib</span> Nonsteroidal anti-inflammatory medication

Celecoxib, sold under the brand name Celebrex among others, is a COX-2 inhibitor and nonsteroidal anti-inflammatory drug (NSAID). It is used to treat the pain and inflammation in osteoarthritis, acute pain in adults, rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, painful menstruation, and juvenile rheumatoid arthritis. It may also be used to decrease the risk of colorectal adenomas in people with familial adenomatous polyposis. It is taken by mouth. Benefits are typically seen within an hour.

Glucosamine (C6H13NO5) is an amino sugar and a prominent precursor in the biochemical synthesis of glycosylated proteins and lipids. Glucosamine is part of the structure of two polysaccharides, chitosan and chitin. Glucosamine is one of the most abundant monosaccharides. Produced commercially by the hydrolysis of shellfish exoskeletons or, less commonly, by fermentation of a grain such as corn or wheat, glucosamine has many names depending on country.

<span class="mw-page-title-main">Methylsulfonylmethane</span> Chemical compound

Dimethyl sulfone (DMSO2) is an organosulfur compound with the formula (CH3)2SO2. It is also known by several other names including methyl sulfone and (especially in alternative medicine) methylsulfonylmethane (MSM). This colorless solid features the sulfonyl functional group and is the simplest of the sulfones. It is relatively inert chemically and is able to resist decomposition at elevated temperatures. It occurs naturally in some primitive plants, is present in small amounts in many foods and beverages, and is marketed (under the MSM name) as a dietary supplement. It is sometimes used as a cutting agent for illicitly manufactured methamphetamine. It is also commonly found in the atmosphere above marine areas, where it is used as a carbon source by the airborne bacteria Afipia. Oxidation of dimethyl sulfoxide produces the sulfone, both under laboratory conditions and metabolically.

A chondroitin is a chondrin derivative.

<span class="mw-page-title-main">Joint injection</span> Method of delivering drugs into a joint

In medicine, a joint injection is a procedure used in the treatment of inflammatory joint conditions, such as rheumatoid arthritis, psoriatic arthritis, gout, tendinitis, bursitis, Carpal Tunnel Syndrome, and occasionally osteoarthritis. A hypodermic needle is injected into the affected joint where it delivers a dose of any one of many anti-inflammatory agents, the most common of which are corticosteroids. Hyaluronic acid, because of its high viscosity, is sometimes used to replace bursa fluids. The technique may be used to also withdraw excess fluid from the joint.

<span class="mw-page-title-main">Pentosan polysulfate</span> Chemical compound

Pentosan polysulfate, sold under the brand name Elmiron among others, is a medication used for the treatment of interstitial cystitis. It was approved for medical use in the United States in 1996.

Chondropathy refers to a disease of the cartilage. It is frequently divided into 5 grades, with 0-2 defined as normal and 3-4 defined as diseased.

Heparinoids are glycosaminoglycans which are chemically and pharmacologically related to heparin. They include oligosaccharides and sulfated polysaccharides of plant, animal, or synthetic origin. Multiple scientific studies have been conducted on heparinoids.

Naproxcinod (nitronaproxen) is a nonsteroidal anti-inflammatory drug (NSAID) developed by the French pharmaceutical company NicOx. It is a derivative of naproxen with a nitroxybutyl ester to allow it to also act as a nitric oxide (NO) donor. This second mechanism of action makes naproxcinod the first in a new class of drugs, the cyclooxygenase inhibiting nitric oxide donators (CINODs), that are hoped to produce similar analgesic efficacy to traditional NSAIDs, but with less gastrointestinal and cardiovascular side effects.

Tanezumab is a monoclonal antibody against nerve growth factor as a treatment for pain via a novel mechanisms different from conventional pain-killer drugs. Tanezumab was discovered and developed by Rinat Neuroscience and was acquired by Pfizer in 2006.

<span class="mw-page-title-main">Sodium hyaluronate</span> Chemical compound

Sodium hyaluronate is the sodium salt of hyaluronic acid, a glycosaminoglycan found in various connective tissue of humans.

<span class="mw-page-title-main">Knee arthritis</span> Medical condition

Arthritis of the knee is typically a particularly debilitating form of arthritis. The knee may become affected by almost any form of arthritis.

Cosequin is a nutritional supplement for animals. It claims to support joints in pets.

Celadrin is an over-the-counter medication marketed as a topical analgesic. It is a cream that is used for muscle and joint pains including osteoarthritis, strains, bruises, and sprains. The active ingredient is menthol and it also contains cetylated and esterified fatty acids. The inactive ingredients are Benzyl Alcohol, Butylparaben, carbomer interpolymer type a, ethylparaben, glycerin, glyceryl monostearate, isobutylparaben, soybean, methylparaben, olive oil, PEG-100 Stearate, peppermint oil, phenoxyethanol, sodium hydroxide, propylparaben, alpha-tocopherol acetate, and water.

Sprifermin (INN), is a recombinant human fibroblast growth factor 18 (rhFGF18) analog, which is under development by TrialSpark for the treatment of osteoarthritis. FGF18 and sprifermin act via the Fibroblast Growth Factor Receptor (FGFR) family, with preferential activity via FGFR3c.

A significant amount of research has been performed on glycosaminoglycans, especially glucosamine and chondroitin, for the treatment of arthritis. These compounds are commonly marketed as nutritional supplements and numerous 'soft therapeutic claims' are made about their health benefits - especially in aging populations. Since glucosamine is a precursor for glycosaminoglycans, and glycosaminoglycans are major components of cartilage, ingesting glucosamine might nourish joints, and thereby alleviate arthritis symptoms. Authoritative opinions on the actual therapeutic value of these compounds have been very mixed.

<span class="mw-page-title-main">Polysulfated glycosaminoglycan</span> Injectable drug

Polysulfated glycosaminoglycan (PSGAG), sold under the brand name Adequan, is an injectable drug for dogs and horses that is used to alleviate the limpness, pain, and lowered range of motion caused by arthritis. It is made of repeat disaccharide units (comprising hexosamine and hexuronic acid), and is similar to glycosaminoglycans already present in the cartilage; PSGAG thus easily integrates itself there. In vitro studies have shown it to inhibit the enzymes that degrade cartilage and bone, as well as suppress inflammation and stimulate the synthesis of replacement cartilage. While it can cause an increased risk of bleeding, it is relatively safe and has a high LD50. PSGAG is one of the most widely prescribed joint treatments for horses.

<span class="mw-page-title-main">Senior dog diet</span> Pet foods catered toward senior pets

Senior dog food diets are pet foods that are catered toward the senior or mature pet population. The senior dog population consists of dogs that are over the age of seven for most dog breeds, though in general large and giant breed dogs tend to reach this life stage earlier when compared to smaller breed dogs. Senior dog foods contain nutrients and characteristics that are used to improve the health of the aging dog. Aging in dogs causes many changes to occur physiologically that will require a change in nutrient composition of their diet.

A disease-modifying osteoarthritis drug (DMOAD) is a disease-modifying drug that would inhibit or even reverse the progression of osteoarthritis. Since the main hallmark of osteoarthritis is cartilage loss, a typical DMOAD would prevent the loss of cartilage and potentially regenerate it. Other DMOADs may attempt to help repair adjacent tissues by reducing inflammation. A successful DMOAD would be expected to show an improvement in patient pain and function with an improvement of the health of the joint tissues.

References

  1. 1 2 McAtee, Caitlin O.; Barycki, Joseph J.; Simpson, Melanie A. (2014-01-01), Simpson, Melanie A.; Heldin, Paraskevi (eds.), "Chapter One – Emerging Roles for Hyaluronidase in Cancer Metastasis and Therapy", Advances in Cancer Research, Hyaluronan Signaling and Turnover, 123, Academic Press: 1–34, doi:10.1016/b978-0-12-800092-2.00001-0, PMC   4445717 , PMID   25081524
  2. ↑ Klecker, Christina; Nair, Lakshmi S. (2017-01-01), Vishwakarma, Ajaykumar; Karp, Jeffrey M. (eds.), "Chapter 13 – Matrix Chemistry Controlling Stem Cell Behavior", Biology and Engineering of Stem Cell Niches, Boston: Academic Press, pp. 195–213, doi:10.1016/b978-0-12-802734-9.00013-5, ISBN   978-0128027349
  3. ↑ Baeurle SA, Kiselev MG, Makarova ES, Nogovitsin EA (2009). "Effect of the counterion behavior on the frictional–compressive properties of chondroitin sulfate solutions". Polymer. 50 (7): 1805–13. doi:10.1016/j.polymer.2009.01.066.
  4. ↑ Schieber, A.; Lopes-Lutz, D. (2011-01-01), Moo-Young, Murray (ed.), "4.40 – Analytical Methods – Functional Foods and Dietary Supplements", Comprehensive Biotechnology (Second Edition), Burlington: Academic Press, pp. 487–99, doi:10.1016/b978-0-08-088504-9.00320-2, ISBN   978-0080885049
  5. 1 2 3 4 5 Singh, JA; Noorbaloochi, S; MacDonald, R; Maxwell, LJ (28 January 2015). Singh, Jasvinder A (ed.). "Chondroitin for osteoarthritis". The Cochrane Database of Systematic Reviews. 1 (4): CD005614. doi:10.1002/14651858.CD005614.pub2. PMC   4881293 . PMID   25629804.
  6. ↑ American Academy of Orthopaedic Surgeons (February 2013), "Five Things Physicians and Patients Should Question", Choosing Wisely: an initiative of the ABIM Foundation , American Academy of Orthopaedic Surgeons, retrieved 19 May 2013, which cites
    • Jevsevar, DS; Brown, GA; Jones, DL; Matzkin, EG; Manner, PA; Mooar, P; Schousboe, JT; Stovitz, S; Sanders, JO; Bozic, KJ; Goldberg, MJ; Martin WR, 3rd; Cummins, DS; Donnelly, P; Woznica, A; Gross, L; American Academy of Orthopaedic Surgeons (Oct 16, 2013). "The American Academy of Orthopaedic Surgeons evidence-based guideline on: treatment of osteoarthritis of the knee, 2nd edition". The Journal of Bone and Joint Surgery. American Volume. 95 (20): 1885–86. doi:10.2106/00004623-201310160-00010. PMID   24288804.{{cite journal}}: CS1 maint: numeric names: authors list (link)
    • Clegg, Daniel O.; Reda, Domenic J.; Harris, Crystal L.; Klein, Marguerite A.; O'Dell, James R.; Hooper, Michele M.; Bradley, John D.; Bingham, Clifton O.; Weisman, Michael H.; Jackson, Christopher G.; Lane, Nancy E.; Cush, John J.; Moreland, Larry W.; Schumacher, H. Ralph; Oddis, Chester V.; Wolfe, Frederick; Molitor, Jerry A.; Yocum, David E.; Schnitzer, Thomas J.; Furst, Daniel E.; Sawitzke, Allen D.; Shi, Helen; Brandt, Kenneth D.; Moskowitz, Roland W.; Williams, H. James (23 February 2006). "Glucosamine, Chondroitin Sulfate, and the Two in Combination for Painful Knee Osteoarthritis". New England Journal of Medicine. 354 (8): 795–808. doi: 10.1056/NEJMoa052771 . PMID   16495392. S2CID   1609605.
    • Richmond, J; Hunter, D; Irrgang, J; Jones, MH; Levy, B; Marx, R; Snyder-Mackler, L; Watters WC, 3rd; Haralson RH, 3rd; Turkelson, CM; Wies, JL; Boyer, KM; Anderson, S; St Andre, J; Sluka, P; McGowan, R; American Academy of Orthopaedic Surgeons (Sep 2009). "Treatment of osteoarthritis of the knee (nonarthroplasty)". The Journal of the American Academy of Orthopaedic Surgeons. 17 (9): 591–600. doi:10.5435/00124635-200909000-00006. PMC   3170838 . PMID   19726743.{{cite journal}}: CS1 maint: numeric names: authors list (link)
  7. ↑ Morrison LM, Enrick N. "Coronary heart disease: reduction of death rate by chondroitin sulfate A". Angiology. 1973 May;24(5):269–87. doi : 10.1177/000331977302400503. PMID   4267673.
  8. ↑ Qi, Shan; Shao, Meng (2021). "Chondroitin Sulfate Alleviates Diabetic Osteoporosis and Repairs Bone Microstructure via Anti-Oxidation, Anti-Inflammation, and Regulating Bone Metabolism". Front Endocrinol. 12: 16. doi: 10.3389/fendo.2021.759843 . PMC   8579055 . PMID   34777254.
  9. ↑ "Chondroitin Sulfate". Stanford Chemicals. Retrieved July 26, 2024.
  10. ↑ Manohar, Krishna; Mesfin, Fikir (2024). "Effect of Oral Chondroitin Sulfate Supplementation on Acute Brain Injury in a Murine Necrotizing Enterocolitis Model". J Am Coll Surg. 238 (1): 82–98. doi:10.1097/XCS.0000000000000896. PMID   37870229.
  11. ↑ 13. Hathcock JN, Shao a. Risk assessment for glucosamine and chondroitin sulfate. Regulatory Toxicology and Pharmacology, 2007; 47: 78–83
  12. ↑ Jordan KM; Recommendations Arden NK. EULAR (2003). "an evidence based approach to the management of knee osteoarthritis: Report of a Task Force of the Standing Committee for International Clinical Studies Including Therapeutic Trials (ESCISIT)". Ann Rheum Dis. 62 (12): 1145–55. doi:10.1136/ard.2003.011742. PMC   1754382 . PMID   14644851.
  13. ↑ Andermann G, Dietz M. The influence of the route of administration on the bioavailability of an endogenous macromolecule: chondroitin sulfate (CSA). Eur J Drug Metab Pharmacokinet 1982;7:11–6
  14. ↑ Monfort, J; Pelletier, JP; Garcia-Giralt, N; Martel-Pelletier, J (June 2008). "Biochemical basis of the effect of chondroitin sulphate on osteoarthritis articular tissues". Annals of the Rheumatic Diseases. 67 (6): 735–40. doi:10.1136/ard.2006.068882. PMID   17644553. S2CID   41984358.
  15. ↑ Conte, A; Palmieri, L; Segnini, D; Ronca, G (1991). "Metabolic fate of partially depolymerized chondroitin sulfate administered to the rat". Drugs Exp Clin Res. 17 (1): 27–33. PMID   1914833.
  16. ↑ Conte, A; de Bernardi, M; Palmieri, L; Lualdi, P; Mautone, G; Ronca, G (1991). "Metabolic fate of exogenous chondroitin sulfate in man". Arzneimittelforschung. 41 (7): 768–72. PMID   1772467.
  17. ↑ Conte, A; Volpi, N; Palmieri, L; Bahous, I; Ronca, G (1995). "Biochemical and pharmacokinetic aspects of oral treatment with chondroitin sulfate". Arzneimittelforschung. 45 (8): 918–25. PMID   7575762.
  18. ↑ Palmieri L, Conte A, Giovannini L, Lualdi P, Ronca G. Metabolic fate of exogenous chondroitin sulfate in the experimental animal" Arzneimittelforschung 1990;40:319–23.
  19. ↑ Ronca F, Palmieri L, Panicucci P, Ronca G. "Anti-inflammatory activity of chondroitin sulfate" Osteoarthritis and Cartilage 1998;6 Suppl A:14–21.
  20. ↑ Valcarcel, Jesus; Novoa-Carballal, Ramon; Pérez-Martín, Ricardo; Reis, Rui L; Vázquez, José Antonio (2017). "Glycosaminoglycans from marine sources as therapeutic agents Authors". Biotechnology Advances. 35 (6): 711–25. doi:10.1016/j.biotechadv.2017.07.008. PMID   28739506.
  21. ↑ Valcarcel, Jesus; García, Míriam R.; Sampayo, Lucia F.; Vázquez, José A. (2020). "Marine chondroitin sulfate of defined molecular weight by enzymatic depolymerization". Carbohydrate Polymers. 229: 115450. doi:10.1016/j.carbpol.2019.115450. hdl: 10261/193588 . PMID   31826487. S2CID   208599006.
  22. ↑ Silbert JE, Sugumaran G (2002). "Biosynthesis of chondroitin/dermatan sulfate". IUBMB Life. 54 (4): 177–86. doi: 10.1080/15216540214923 . PMID   12512856. S2CID   11564009.
  23. ↑ Aravamudhan, Aja; Ramos, Daisy M.; Nada, Ahmed A.; Kumbar, Sangamesh G. (2014-01-01), Kumbar, Sangamesh G.; Laurencin, Cato T.; Deng, Meng (eds.), "Chapter 4 – Natural Polymers: Polysaccharides and Their Derivatives for Biomedical Applications", Natural and Synthetic Biomedical Polymers, Oxford: Elsevier, pp. 67–89, doi:10.1016/b978-0-12-396983-5.00004-1, ISBN   978-0123969835
  24. ↑ P. A. Levene; F. B. La Forge (1913). "On Chondroitin Sulphuric Acid". J. Biol. Chem. 15: 69–79. doi: 10.1016/S0021-9258(18)88542-8 . Free PDF online Archived 2008-11-05 at the Wayback Machine
  25. ↑ Chondroitin+sulfates at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
  26. ↑ Davidson EA, Meyer K (1954). "Chondroitin, a new mucopolysaccharide". J Biol Chem. 211 (2): 605–11. doi: 10.1016/S0021-9258(18)71150-2 . PMID   13221568. Free PDF online Archived 2008-09-22 at the Wayback Machine
  27. 1 2 Barnhill JG, Fye CL, Williams DW, Reda DJ, Harris CL, Clegg DO (2006). "Chondroitin product selection for the glucosamine/chondroitin arthritis intervention trial". Journal of the American Pharmacists Association. 46 (1): 14–24. doi:10.1331/154434506775268616. PMID   16529337.
  28. ↑ Zawisza, Julie (2008-03-19). "FDA Media Briefing on Heparin" (PDF). U.S. Food and Drug Administration . Retrieved 2008-04-23.
  29. ↑ Guerrini, M; Beccati, D; Shriver, Z; Naggi, A; Viswanathan, K; Bisio, A; Capila, I; Lansing, JC; Guglieri, S; Fraser, B; Al-Hakim, A; Gunay, NS; Zhang, Z; Robinson, L; Buhse, L; Nasr, M; Woodcock, J; Langer, R; Venkataraman, G; Linhardt, RJ; Casu, B; Torri, G; Sasisekharan, R (June 2008). "Oversulfated chondroitin sulfate is a contaminant in heparin associated with adverse clinical events". Nature Biotechnology. 26 (6): 669–75. doi:10.1038/nbt1407. PMC   3491566 . PMID   18437154.
  30. ↑ Kishimoto, TK; Viswanathan, K; Ganguly, T; Elankumaran, S; Smith, S; Pelzer, K; Lansing, JC; Sriranganathan, N; Zhao, G; Galcheva-Gargova, Z; Al-Hakim, A; Bailey, GS; Fraser, B; Roy, S; Rogers-Cotrone, T; Buhse, L; Whary, M; Fox, J; Nasr, M; Dal Pan, GJ; Shriver, Z; Langer, RS; Venkataraman, G; Austen, KF; Woodcock, J; Sasisekharan, R (5 June 2008). "Contaminated heparin associated with adverse clinical events and activation of the contact system". The New England Journal of Medicine. 358 (23): 2457–67. doi:10.1056/NEJMoa0803200. PMC   3778681 . PMID   18434646.
  31. 1 2 3 Letter Regarding the Relationship Between the Consumption of Glucosamine and/or Chondroitin Sulfate and a Reduced Risk of: Osteoarthritis; Osteoarthritis-related Joint Pain, Joint Tenderness, and Joint Swelling; Joint Degeneration; and Cartilage Deterioration(Docket No. 2004P-0059). Addressed to John W. Emford, Esq. William K. Hubbard. Oct. 7, 2004. <https://www.fda.gov/food/ingredientspackaginglabeling/labelingnutrition/ucm073400.htm>
  32. ↑ Clegg, DO; et al. (2006). "Glucosamine, chondroitin sulfate, and the two in combination for painful knee osteoarthritis". N Engl J Med. 354 (8): 795–808. doi: 10.1056/nejmoa052771 . PMID   16495392. S2CID   1609605.
  33. 1 2 Sawitzke, Allen D.; Shi, Helen; Finco, Martha F.; Dunlop, Dorothy D.; Harris, Crystal L.; Singer, Nora G.; Bradley, John D.; Silver, David; Jackson, Christopher G.; Lane, Nancy E.; Oddis, Chester V.; Wolfe, Fred; Lisse, Jeffrey; Furst, Daniel E.; Bingham, Clifton O.; Reda, Domenic J.; Moskowitz, Roland W.; Williams, H. James; Clegg, Daniel O. (4 June 2010). "Clinical efficacy and safety of glucosamine, chondroitin sulphate, their combination, celecoxib or placebo taken to treat osteoarthritis of the knee: 2-year results from GAIT". Annals of the Rheumatic Diseases. 69 (8): 1459–64. doi:10.1136/ard.2009.120469. PMC   3086604 . PMID   20525840.
  34. ↑ Mazzucchelli, Ramón; Rodríguez-Martín, Sara; García-Vadillo, Alberto; Gil, Miguel; Rodríguez-Miguel, Antonio; Barreira-Hernández, Diana; García-Lledó, Alberto; de Abajo, Francisco J. (2021-07-12). "Risk of acute myocardial infarction among new users of chondroitin sulfate: A nested case-control study". PLOS ONE. 16 (7): e0253932. Bibcode:2021PLoSO..1653932M. doi: 10.1371/journal.pone.0253932 . ISSN   1932-6203. PMC   8274913 . PMID   34252115.
  35. ↑ Barnhill, JG; Fye, CL; Williams, DW; Reda, DJ; Harris, CL; Clegg, DO (2006). "Chondroitin product selection for the glucosamine/chondroitin arthritis intervention trial". Journal of the American Pharmacists Association. 46 (1): 14–24. doi:10.1331/154434506775268616. PMID   16529337.
  36. ↑ "Questions and Answers: NIH Glucosamine/Chondroitin Arthritis Intervention Trial Primary Study", Backgrounder, National Center for Complementary and Integrative Medicine, 2006-01-02, retrieved 2013-10-17
  37. ↑ Vergés J, Castañeda-Hernández, G. On the bioavailability of oral chondroitin sulfate formulations: proposed criteria for bioequivalence studies. Proc. West. Pharmacol. Soc., 2004; 47: 50–53
  38. ↑ Adebowale AO Cox DS, Liang Z, Eddington ND (2000). "Analysis of glucosamine and chondroitin sulfate content in marketed products and the Caco-2 permeability of chondroitin sulfate raw materials". J Am Nutr Assoc. 3: 37–44. Archived from the original on April 7, 2006.
  39. ↑ Bhathal, A; Spryszak, M; Louizos, C; Frankel, G (2017). "Glucosamine and chondroitin use in canines for osteoarthritis: A review". Open Veterinary Journal. 7 (1): 36–49. doi:10.4314/ovj.v7i1.6. PMC   5356289 . PMID   28331832.
  40. ↑ Bennett, D; Zainal Ariffin, SM; Johnston, P (January 2012). "Osteoarthritis in the cat: 2. how should it be managed and treated?". Journal of Feline Medicine and Surgery. 14 (1): 76–84. doi:10.1177/1098612X11432829. PMC   11148914 . PMID   22247327. S2CID   206691363.
  41. ↑ Goodrich, LR; Nixon, AJ (January 2006). "Medical treatment of osteoarthritis in the horse – a review". Veterinary Journal. 171 (1): 51–69. doi:10.1016/j.tvjl.2004.07.008. PMID   16427582.
This page is based on this Wikipedia article
Text is available under the CC BY-SA 4.0 license; additional terms may apply.
Images, videos and audio are available under their respective licenses.