| Stizolobate synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.13.11.29 | ||||||||
| CAS no. | 65979-39-7 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Stizolobate synthase (EC 1.13.11.29) is an enzyme that catalyzes the chemical reaction
The two substrates of this enzyme are L-DOPA and oxygen. Its initial product is 4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde. [1]
The intermediate readily cyclises and further oxidises, forming a 2-pyrone ring; this stizolobic acid, which is the product that is isolated and after which this enzyme is named. [2] [3]
The oxidation step uses oxidised nicotinamide adenine dinucleotide as a cofactor and the enzyme also requires zinc. It belongs to the family of oxidoreductases, specifically those acting on single donors with O2 as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases). The oxygen incorporated need not be derived from O2. The systematic name of this enzyme class is 3,4-dihydroxy-L-phenylalanine:oxygen 4,5-oxidoreductase (recyclizing). It participates in tyrosine metabolism. [1]
A separate enzyme, stizolobinate synthase also present in the plant cleaves L-DOPA in an way which produces the isomeric compound, stizolobinic acid. [3]
