Tobacco etch virus

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Tobacco etch virus
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Symptoms of tobacco etch virus in burley tobacco ( Nicotiana tabacum )
Virus classification OOjs UI icon edit-ltr.svg
(unranked): Virus
Realm: Riboviria
Kingdom: Orthornavirae
Phylum: Pisuviricota
Class: Stelpaviricetes
Order: Patatavirales
Family: Potyviridae
Genus: Potyvirus
Species:
Tobacco etch virus
Synonyms

datura Z virus
tobacco severe etch virus
tomato etch virus

Contents

Tobacco etch virus (TEV) is a plant virus in the genus Potyvirus and family Potyviridae . [1] Like other members of the genus Potyvirus, TEV has a monopartite positive-sense, single-stranded RNA genome surrounded by a capsid made from a single viral encoded protein. The virus is a filamentous particle that measures about 730 nm in length. It is transmissible in a non-persistent manner by more than 10 species of aphids including Myzus persicae . It also is easily transmitted by mechanical means but is not known to be transmitted by seeds. [2]

Host range

This virus infects many species of Solanaceae. Agriculturally important crops that it infects include several species of Capsicum (i.e. C. annuum , C. frutescens ), tomato ( Lycopersicon esculentum ), and tobacco ( Nicotiana spp.).

It also infects many perennial weed species that can act as virus reservoirs for susceptible agricultural crops. These weed species include Solanum nigrum (nightshade), S. aculeatissimum (soda apple), Chenopodium album (pigweed), Datura stramonium (jimson weed), Linaria canadensis (blue toadflax), and Physalis spp. (ground cherry). Thus, recommendations for the control of this virus include the control of weeds in and around susceptible solanaceous crops. [3]

Signs and symptoms

Symptoms seen on plants infected with this virus can vary depending on the plant. However typical symptoms include vein clearing, mottling, and necrotic lines or etching. Symptoms can occur on leaves and fruit and the plants can become stunted. [4]

Like other potyviruses, TEV makes viral inclusions that can be seen in the light microscope if properly stained. [5] [6] [7] This particular potyvirus makes two kinds of inclusions that can be diagnostic in a known host. One of the inclusions is the cylindrical inclusions found in the cytoplasm of infected cells and the second inclusion is found in the nucleus. Neither inclusion type stains in the nucleic acid stain (AzureA). (Inclusions of tobacco etch virus in various hosts.)

Geographic distribution

TEV appears to be a virus that evolved in the New World. [8] It has been reported in Canada, the United States (including Hawaii), Mexico and Puerto Rico in North America and in Venezuela in South America. [2]

Beyond plant disease

Potyvirus RNA codes for at least seven different proteins. [9] One of them is a protease. The TEV protease is a highly site-specific protease that biochemists have used to their advantage to create a protein purification system by incorporating TEV protease's recognition site into protein purification tags.

A gene construct is created containing the protein of interest fused to a TEV protease recognition site, followed by an affinity tag, such as a polyhistidine-tag. Following affinity chromatography, the purified protein is then treated with TEV protease. TEV protease cleaves at its recognition site, removing the affinity tag. This allows for affinity purification of proteins that are not well-behaved when fused to protein tags. [10]

Related Research Articles

<i>Potyviridae</i> Family of viruses

Potyviridae is a family of positive-strand RNA viruses that encompasses more than 30% of known plant viruses, many of which are of great agricultural significance. The family has 12 genera and 235 species, three of which are unassigned to a genus.

<i>Potyvirus</i> Genus of positive-strand RNA viruses in the family Potyviridae

Potyvirus is a genus of positive-strand RNA viruses in the family Potyviridae. Plants serve as natural hosts. Like begomoviruses, members of this genus may cause significant losses in agricultural, pastoral, horticultural, and ornamental crops. More than 200 species of aphids spread potyviruses, and most are from the subfamily Aphidinae. The genus contains 190 species and potyviruses account for about thirty percent of all currently known plant viruses.

<span class="mw-page-title-main">TEV protease</span> Highly specific protease

TEV protease is a highly sequence-specific cysteine protease from Tobacco Etch Virus (TEV). It is a member of the PA clan of chymotrypsin-like proteases. Due to its high sequence specificity, TEV protease is frequently used for the controlled cleavage of fusion proteins in vitro and in vivo.

<i>Bidens mottle virus</i> Species of virus

Bidens mottle virus (BiMoV) is a pathogenic plant virus in the plant virus family Potyviridae. BiMoV is a flexuous filamentous particle, 720 nm long, and belongs to the Potyviridae genus Potyvirus. Like other viruses in this genus, Bidens mottle virus is transmitted both mechanically by sap and by aphids in a stylet-borne fashion.

Papaya mosaic virus (PapMV) is a plant pathogenic virus in the genus Potexvirus and the family Alphaflexiviridae. PapMV is a filamentous, flexuous rod, 530 nm in length.

<i>Peanut mottle virus</i> Species of virus

Peanut mottle virus(PeMoV) is a pathogenic plant virus of the family Potyviridae. As with other members of this virus family, PeMoV is a flexuous filamentous virus with particles 740–750 nm long. It is transmitted by several species of aphids and by mechanical inoculation. It was first given its name in 1965 when it was isolated from peanuts (Arachis hypogaea) in Georgia, United States. The virus was found to be seed transmitted in its host.

Pepper mottle virus (PepMoV) is a plant pathogenic virus in the genus Potyvirus and the virus family Potyviridae. Like other members of the Potyvirus genus, PepMV is a monopartite strand of positive-sense, single-stranded RNA surrounded by a capsid made for a single viral encoded protein. The virus is a filamentous particle that measures about 737 nm in length. Isolates of this virus has been completely sequenced and its RNA is 9640 nucleotides long. This virus is transmitted by several species of aphids in a nonpersitant manner and by mechanical inoculation.

Potato virus Y (PVY) is a plant pathogenic virus of the family Potyviridae, and one of the most important plant viruses affecting potato production.

White clover mosaic virus (WClMV) is a plant pathogenic virus in the genus Potexvirus and the family Alphaflexiviridae. WClMV is a filamentous, flexuous rod, 480 nm in length and 13 nm wide.

<i>Tobacco ringspot virus</i> Species of virus

Tobacco ringspot virus (TRSV) is a plant pathogenic virus in the plant virus family Secoviridae. It is the type species of the genus Nepovirus. Nepoviruses are transmitted between plants by nematodes, thrips, mites, grasshoppers, and flea beetles. TRSV is also easily transmitted by sap inoculation and transmission in seeds has been reported. In recent cases it has also been shown to appear in bees, but no transmission to plants from bees has been noted.

Tradescantia mosaic virus (TZV) is a plant pathogenic virus in the genus Potyvirus and the virus family Potyviridae. Like other members of the Potyvirus genus, TZV is a monopartite strand of positive-sense, single-stranded RNA surrounded by a capsid made for a single viral encoded protein. The virus is a filamentous particle that measures about 754 nanometers in length. This virus is transmitted by two species of aphids, Myzus persicae and Rhapalosiphum padi and by mechanical inoculation.

Sweet potato feathery mottle virus (SPFMV) is a member of the genus Potyvirus in the family Potyviridae. It is most widely recognized as one of the most regularly occurring causal agents of sweet potato viral disease (SPVD) and is currently observed in every continent except Antarctica. The number of locations where it is found is still increasing; generally, it is assumed that the virus is present wherever its host is. The virus has four strains that are found in varying parts of the world.

Apium virus Y (ApVY) is a plant pathogenic virus in the genus Potyvirus and the virus family Potyviridae.

Commelina mosaic virus (CoMV) is a plant pathogenic virus in the genus Potyvirus and the virus family Potyviridae. Like other members of the Potyvirus genus, CoMV is a monopartite strand of positive-sense, single-stranded RNA surrounded by a capsid made for a single viral encoded protein. The virus is a filamentous particle that measures about 707-808 nm in length. This virus is transmitted by two species of aphids, Myzus persicae and Aphis gossypii, and by mechanical inoculation.

Clover yellow vein virus (ClYVV) is a plant pathogenic virus in the genus Potyvirus and the virus family Potyviridae. Like other members of the Potyvirus genus, ClYVV is a monopartite strand of positive-sense, single-stranded RNA surrounded by a capsid made for a single viral encoded protein. The virus is a filamentous particle that measures about 760 nm in length. This virus is transmitted by several species of aphids in a nonpersistent manner and by mechanical inoculation.

Passion fruit woodiness virus (PWV) is a plant pathogenic virus in the genus Potyvirus and the virus family Potyviridae. Like other members of the genus Potyvirus, PWV is a monopartite strand of positive-sense, single-stranded RNA surrounded by a capsid made for a single viral encoded protein. The virus is a filamentous particle that measures about 745 nm in length.

<i>Watermelon mosaic virus</i> Species of virus

Watermelon mosaic virus (WMV) also known as Marrow mosaic virus, Melon mosaic virus, and until recently Watermelon mosaic virus type 2 (WMV-2), is a plant pathogenic virus that causes viral infection in many different plants. The virus itself is referred to as Watermelon Mosaic Virus II or WMV-2 and is an isolate of the U.S. WMV-2 is a ssRNA positive strand virus that is part of the Potyviridae or Potyvirus clade. Like all RNA viruses, it contains a protein capsid which protects the inner viral RNA. First described on squash in Florida, WMV arose from a unique recombination of genetic material contributed by Soybean mosaic virus (SMV) and Bean common mosaic virus (BCMV) along with Peanut Stripe virus (PSV).

Nuclear-inclusion-a endopeptidase is a protease enzyme found in potyviruses. This enzyme catalyses the following chemical reaction:

The cardamom mosaic virus (CdMV) is a mosaic virus that affects the production of green cardamom (E. cardamomum). It is a member of the genus Macluravirus (recognized under the family Potyviridae by ICTV in 1988), and is transmitted through aphids (P.caladii) and infected rhizomes, the former in a non-persistent manner.

Carrot virus Y (CarVY) is a (+)ss-RNA virus that affects crops of the carrot family (Apiaceae), such as carrots, anise, chervil, coriander, cumin, dill and parsnip. Carrots are the only known crop to be infected in the field. Infection by the virus leads to deformed roots and discolored or mottled leaves. The virus is spread through insect vectors, and is currently only found in Australia.

References

  1. "About Plant Viruses / Material and Methods for the Detection of Viral Inclusions / Florida Plant Viruses and Their Inclusions / Science / Plant Industry / Divisions & Offices / Home - Florida Department of Agriculture & Consumer Services". www.freshfromflorida.com.
  2. 1 2 Antoniw, John. "Show DPV and Refs in Frame". www.dpvweb.net.
  3. L. L. Breman (July 1987). "Tobacco Etch Virus" (PDF). Plant Pathology Circular No. 297 Fla. Dept. Agric. & Consumer Serv. Retrieved July 12, 2017.
  4. "Tobacco Etch Virus Potyvirus TEV". www.invasive.org.
  5. "Material and Methods for the Detection of Viral Inclusions / Florida Plant Viruses and Their Inclusions / Science / Plant Industry / Divisions & Offices / Home - Florida Department of Agriculture & Consumer Services". www.freshfromflorida.com. Archived from the original on 2014-10-13.
  6. Christie, R.G. and Edwardson, J.R. (1977). Fla Agric. Exp. Stn Monog. No. 9, 150 pp.
  7. "Diagnosis / Material and Methods for the Detection of Viral Inclusions / Florida Plant Viruses and Their Inclusions / Science / Plant Industry / Divisions & Offices / Home - Florida Department of Agriculture & Consumer Services". www.freshfromflorida.com.
  8. Gibbs A, Ohshima K (2010). "Potyviruses and the digital revolution". Annu Rev Phytopathol. 48: 205–23. doi:10.1146/annurev-phyto-073009-114404. PMID   20438367.
  9. Adam C. Winterhalter. "Potyvirus". Archived from the original on 2005-07-20.
  10. Jenny RJ, Mann KG, Lundblad RL (2003). "A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa". Protein Expr. Purif. 31 (1): 1–11. doi:10.1016/S1046-5928(03)00168-2. PMID   12963335.