Vampyrella lateritia

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Vampyrella lateritia
Vampyrella lateritia.jpg
Vampyrella lateritia
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Eukaryota
Clade: Diaphoretickes
Clade: SAR
Phylum: Endomyxa
Class: Vampyrellidea
Order: Vampyrellida
Family: Vampyrellidae
Genus: Vampyrella
Species:
V. lateritia
Binomial name
Vampyrella lateritia
Fresenius, 1856
Synonyms

Amoeba lateritia

Vampyrella lateritia is a freshwater species of predatory amoebae that feeds on species of algae and is known for its specialized feeding strategy of removing, digesting, and ingesting the cellular contents of its prey. [1] [2] It is the type species of the genus Vampyrella and has been identified in numerous locations around the world including Brazil, Germany, and the eastern United States. [3] [4] Along with Vampyrella pendula, its genome was sequenced in 2012. [5]

Contents

Life cycle

Vampyrella lateritia has four life stages that revolve about the feeding cycle: motile trophozoites (the activated, feeding stage), plasmodia in which the cytoplasm contains many nuclei, digestive cysts, and resting cysts. [4] It has been observed feeding on species from the genera Zygnema , Spirogyra , and Mougeotia and is considered a specialist predator as its known prey is restricted to a limited number of green algal species. [4] [6] [7]

Like other vampyrellids, Vampyrella lateritia grows well between 10°C and room temperature. It contains intracellular bacteria that have not yet been conclusively identified, although the morphology of the endosymbiotic bacteria resembles Ca. Megaira polyxenopila, a species of bacteria in the family Rickettsiaceae. [8] [9]

Trophozoites

In this stage of action and feeding, the cells are compact and spherical with radiating filopodia and pseudopodia, moving freely through the water column. The central cell body is orange and the pseudopodia are colourless. In order to move, the filopodia are positioned under the spherical body and slowly rolls the entire cell. [7] Along the pseudopodia, numerous granules known as membranosomes shoot rapidly out of the cell cortex, connected by a thin strand of cytoplasm, and are retracted. [9]

Trophozoites attach to an algal cell and retract their long pseudopodia, flattening the cell body tightly against the algae to increase the contact area. [10] Feeding is proceeded by the dissolution of a hole 5-7 μm in diameter in the algae's cell wall. [11] After several minutes of this, the cell wall bursts and the exposed protoplast is engulfed into a large food vacuole. [10] This process is known as plasmoptysis and resembles a sucking motion. It is likely the origin of the genus name Vampyrella, Latin for 'small vampire'. [4] The remains of the destroyed protoplast still within the algal cell are then engulfed by an ingestion pseudopodium. Vampyrella lateritia can consume several algal cells before entering the digestive cyst phase. After absorbing a single algal cell, Vampyrella lateritia is about ten times its original volume. [12] However the dramatic increase in volume is only temporary and the amoeba returns to its normal volume within a few minutes. Vampyrella lateritia are known to only feed on live prey. [12]

Plasmodia

Individual amoeba can fuse into large and deformed plasmodia. The structure and colour are the same as the trophozoites, however this stage occurs predominantly in old cultures of Vampyrella lateritia where cell density is high and nutrients are limited. [1] However this stage has been observed in laboratory settings, so it is unclear the conditions under which Vampyrella lateritia would form plasmodia in natural conditions.

Digestive cysts

During digestive cyst formation, the trophozoite retracts its pseudopodia and secretes a cell wall. The digestive cysts have two cyst envelopes, where the inner is stronger than the outer. [4] Unlike other vampyrellid amoebae, Vampyrella lateritia retains its separate food vacuoles throughout the entire digestive phase. [1] The digestive phase is marked by a colour change of the cytoplasm as nutrients are digested - as a result, the colour of the digestive cyst is a good indicator for the cyst's maturity. Vampyrella lateritia is often seen as red or orange. [13] After digestion is complete, trophozoites hatch and leave the parent cell through holes in the cell wall created as part of the exocytosis of food remnants.

Although the feeding process only lasts for several minutes in Vampyrella lateritia, the digestive process takes much longer, typically one to two days.

Resting cysts

As resting cysts, Vampyrella lateritia can survive freezing and desiccation for at least three years. Resting cysts have condensed contents and numerous cell walls, however the resting cyst stage of life is not an obligatory part of all Vampyrella lateritia life cycle, unlike the digestive cyst phase. [1] The Vampyrella lateritia can return to the trophozoite phase in the presence of algae prey organisms and fresh medium.

Related Research Articles

<span class="mw-page-title-main">Vampyrellidae</span> Family of single-celled organisms

The family Vampyrellidae is a subgroup of the order Vampyrellida within the supergroup Rhizaria. Based on molecular sequence data, the family currently comprises the genus Vampyrella, and maybe several other vampyrellid amoebae. The cells are naked and characterised by radiating, filose pseudopodia and an orange colouration of the main cell body.

<span class="mw-page-title-main">Pseudopodia</span> False leg found on slime molds, archaea, protozoans, leukocytes and certain bacteria

A pseudopod or pseudopodium is a temporary arm-like projection of a eukaryotic cell membrane that is emerged in the direction of movement. Filled with cytoplasm, pseudopodia primarily consist of actin filaments and may also contain microtubules and intermediate filaments. Pseudopods are used for motility and ingestion. They are often found in amoebas.

<i>Naegleria</i> Genus of protists

Naegleria is a genus consisting of 47 described species of protozoa often found in warm aquatic environments as well as soil habitats worldwide. It has three life cycle forms: the amoeboid stage, the cyst stage, and the flagellated stage, and has been routinely studied for its ease in change from amoeboid to flagellated stages. The Naegleria genera became famous when Naegleria fowleri, the causative agent of the usually fatal human and animal disease primary amoebic meningoencephalitis (PAM), was discovered in 1965. Most species in the genus, however, are incapable of causing disease.

<span class="mw-page-title-main">Rhizaria</span> Infrakingdom of protists

The Rhizaria are a diverse and species-rich supergroup of mostly unicellular eukaryotes. Except for the Chlorarachniophytes and three species in the genus Paulinella in the phylum Cercozoa, they are all non-photosynthethic, but many foraminifera and radiolaria have a symbiotic relationship with unicellular algae. A multicellular form, Guttulinopsis vulgaris, a cellular slime mold, has been described. This group was used by Cavalier-Smith in 2002, although the term "Rhizaria" had been long used for clades within the currently recognized taxon.

<span class="mw-page-title-main">Protozoa</span> Single-celled eukaryotic organisms that feed on organic matter

Protozoa are a polyphyletic group of single-celled eukaryotes, either free-living or parasitic, that feed on organic matter such as other microorganisms or organic debris. Historically, protozoans were regarded as "one-celled animals".

<span class="mw-page-title-main">Thecofilosea</span> Class of single-celled organisms

Thecofilosea is a class of unicellular testate amoebae belonging to the phylum Cercozoa. They are amoeboflagellates, organisms with flagella and pseudopodia, distinguished from other cercozoa by their scale-lacking test composed of organic material. They are closely related to the Imbricatea, a group of testate amoebae with tests composed of inorganic silica scales.

<span class="mw-page-title-main">Plasmodium (life cycle)</span> Living structure of cytoplasm that contains many nuclei

A plasmodium is a living structure of cytoplasm that contains many nuclei, rather than being divided into individual cells each with a single nucleus.

<span class="mw-page-title-main">Vampyrellida</span> Order of single-celled organisms

The vampyrellids, colloquially known as vampire amoebae, are a group of free-living predatory amoebae classified as part of the lineage Endomyxa. They are distinguished from other groups of amoebae by their irregular cell shape with propensity to fuse and split like plasmodial organisms, and their life cycle with a digestive cyst stage that digests the gathered food. They appear worldwide in marine, brackish, freshwater and soil habitats. They are important predators of an enormous variety of microscopic organisms, from algae to fungi and animals. They are also known as aconchulinid amoebae.

<i>Reticulomyxa</i> Genus of single-celled organisms

Reticulomyxa is a monospecific genus of freshwater foraminiferans. The type species is the unicellular Reticulomyxa filosa. It is found in freshwater environments as well as moist environments, like decomposing matter and damp soils. The heterotrophic naked foraminiferan can feed on microbes as well has larger organisms and is able to be sustained in culture by supplemented nutrients such as wheat germ and oats. The large, multinucleate foraminferan is characteristic for its lack of test and named for the network of connecting pseudopodia surrounding its central body mass. The organism has unique bidirectional cytoplasmic streaming throughout the anastomosing pseudopodia that is some of the fastest reported organelle transport observed. Reticulomyxa was first described in 1949 and is commonly used as a model organism for the unique transport of organelles throughout the cytoplasm of pseudopodia by cytoskeletal mechanisms. Only asexual reproduction of this genus has been observed in culture, but the genome possesses genes related to meiosis suggesting it is capable of sexually reproductive life stages.

Rigifila is a genus of free-living single-celled eukaryotes, or protists, containing the sole species Rigifila ramosa. It is classified within the monotypic family Rigifilidae. Along with Micronucleariidae, it is a member of Rigifilida, an order of basal eukaryotes within the CRuMs clade. It differs from Micronuclearia by having two proteic layers surrounding their cytoplasm instead of a single one, and having more irregular mitochondrial cristae, among other morphological differences.

<span class="mw-page-title-main">Amoeba</span> Cellular body type

An amoeba, often called an amoeboid, is a type of cell or unicellular organism with the ability to alter its shape, primarily by extending and retracting pseudopods. Amoebae do not form a single taxonomic group; instead, they are found in every major lineage of eukaryotic organisms. Amoeboid cells occur not only among the protozoa, but also in fungi, algae, and animals.

<i>Kraken</i> (Cercozoa) Genus of single-celled organisms

Kraken is a genus of amoebae within the Cercozoa, containing the sole species Kraken carinae. These amoebae are characterized by a small round cell body and a network of thin and very long filopodia that can reach up to a mm in diameter. Kraken amoebae feed on bacteria and live in freshwater and soil systems.

<i>Vampyrella</i> Genus of single-celled organisms

Vampyrella is a genus of amoebae belonging to the vampyrellid cercozoans usually ranging from 30-60 μm. Members of the genus alternate between two life stages: a free-living trophozoite stage and a cyst stage in which mitosis occurs. This taxon has received a great deal of attention due to their peculiar feeding behaviour of perforating the cell wall of algal cells and drawing out the contents for nourishment.

<i>Maullinia</i> Genus of intracellular parasites

Maullinia is a genus of intracellular, phytomyxid parasites found across the Southern Hemisphere though primarily in Chile, The Prince Edward Islands, South Africa, Australia, and New Zealand. These parasites infiltrate the cells of their brown algal hosts via cytoplasmic extensions called plasmodia that divide synchronously, becoming increasingly multi-nucleate and engulfing the host cell organelles as they grow. Eventually, as the plasmodia fill the entire cell volume, the host cells become hypertrophied and grow to 3- 4x their original size, showing up as swollen appendages or galls on the host tissue at a macroscopic level. These swollen regions will burst alongside the mature Maullinia plasmodia, releasing biflagellated zoospores to the inter- and extracellular space to disperse the infection further. Zoospores can come from sporangial plasmodia, as in M. ectocarpii, or from resting spores, as in M. braseltonii.

<i>Dracoamoeba</i> Genus of naked amoebae

Dracoamoeba is a free-living genus of discosean amoebae in the family Acanthamoebidae containing the sole species Dracoamoeba jomungandri, discovered in 2016 in moist muddy soil close to the ocean shore at Chincoteague, Virginia.

<span class="mw-page-title-main">Viridiraptoridae</span> Family of predatorial protists

Viridiraptoridae, previously known as clade X, is a clade of heterotrophic protists in the phylum Cercozoa. They're a family of glissomonads, a group containing a vast, mostly undescribed diversity of soil and freshwater organisms.

<i>Orciraptor</i> Genus of predatorial protists

Orciraptor is a genus of heterotrophic protists, containing the single species Orciraptor agilis. It belongs to the family Viridiraptoridae, in the phylum Cercozoa.

<i>Aphelidium tribonemae</i> Species of eukaryote

Aphelidium tribonemae is a species within the Aphelid group. Their classification in the kingdom Fungi is a subject of controversy. Some argue for the classification of aphelids as ‘fungal animals', and for a period of time in the 1950s, aphids were classified as protists due to their amoeboid stage. Recently, molecular phylogenetics placed the aphelids within Opisthosporidia, a super phylum within Opisthokonta. Aphelids have posterior uniflagellate zoospores which place them as Opisthokonts. They are an early diverging lineage in Kingdom Fungi. While the aphelid group only contains three genera, it spans many both freshwater and marine ecosystems.

<i>Syssomonas</i> Genus of protists

Syssomonas is a monotypic genus of unicellular flagellated protists containing the species Syssomonas multiformis. It is a member of Pluriformea inside the lineage of Holozoa, a clade containing animals and their closest protistan relatives. It lives in freshwater habitats. It has a complex life cycle that includes unicellular amoeboid and flagellated phases, as well as multicellular aggregates, depending on the growth medium and nutritional state.

Synchroma is a genus of marine stramenopile algae containing two species with amoeboid morphology. They are grouped within the monotypic family Synchromaceae and order Synchromales as part of an independent clade of ochrophytes known as Picophagea or Synchromophyceae.

References

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