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The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. The label GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria and is sometimes called avGFP. However, GFPs have been found in other organisms including corals, sea anemones, zoanithids, copepods and lancelets.
Bioluminescence is the production and emission of light by living organisms. It is a form of chemiluminescence. Bioluminescence occurs widely in marine vertebrates and invertebrates, as well as in some fungi, microorganisms including some bioluminescent bacteria, and terrestrial arthropods such as fireflies. In some animals, the light is bacteriogenic, produced by symbiotic bacteria such as those from the genus Vibrio; in others, it is autogenic, produced by the animals themselves.
Chemiluminescence is the emission of light (luminescence) as the result of a chemical reaction, i.e. a chemical reaction results in a flash or glow of light. A standard example of chemiluminescence in the laboratory setting is the luminol test. Here, blood is indicated by luminescence upon contact with iron in hemoglobin. When chemiluminescence takes place in living organisms, the phenomenon is called bioluminescence. A light stick emits light by chemiluminescence.
A glow stick, also known as a light stick, chem light, light wand, light rod, and rave light, is a self-contained, short-term light-source. It consists of a translucent plastic tube containing isolated substances that, when combined, make light through chemiluminescence. The light cannot be turned off and can be used only once. The used tube is then thrown away. Glow sticks are often used for recreation, such as for events, camping, outdoor exploration, and concerts. Glow sticks are also used for light in military and emergency services applications. Industrial uses include marine, transportation, and mining.
Phosphorescence is a type of photoluminescence related to fluorescence. When exposed to light (radiation) of a shorter wavelength, a phosphorescent substance will glow, absorbing the light and reemitting it at a longer wavelength. Unlike fluorescence, a phosphorescent material does not immediately reemit the radiation it absorbs. Instead, a phosphorescent material absorbs some of the radiation energy and reemits it for a much longer time after the radiation source is removed.
Oryx and Crake is a 2003 novel by Canadian author Margaret Atwood. She has described the novel as speculative fiction and adventure romance, rather than pure science fiction, because it does not deal with things "we can't yet do or begin to do", yet goes beyond the amount of realism she associates with the novel form. It focuses on a lone character called Snowman, who finds himself in a bleak situation with only creatures called Crakers to keep him company. The reader learns of his past, as a boy called Jimmy, and of genetic experimentation and pharmaceutical engineering that occurred under the purview of Jimmy's peer, Glenn "Crake".
Eduardo Kac is a Brazilian and American contemporary artist whose portfolio encompasses various forms of art including performance art, poetry, holography, interactive art, digital and online art, and BioArt. Recognized for his space art and transgenic works, Kac works with biotechnology to create organisms with new genetic attributes. His interdisciplinary approach has seen the use of diverse mediums, from fax and photocopying to fractals, RFID implants, virtual reality, networks, robotics, satellites, telerobotics, virtual reality and DNA synthesis.
A Geissler tube is a precursor to modern gas discharge tubes, demonstrating the principles of electrical glow discharge, akin to contemporary neon lights, and central to the discovery of the electron. This device was developed in 1857 by Heinrich Geissler, a German physicist and glassblower. A Geissler tube is composed of a sealed glass cylinder of various shapes, which is partially evacuated and equipped with a metal electrode at each end. It contains rarefied gases—such as neon or argon, air, mercury vapor, or other conductive substances, and sometimes ionizable minerals or metals like sodium. When a high voltage is applied between the electrodes, there is an electric current through the tube, causing gas molecules to ionize by shedding electrons. The free electrons reunite with the ions and the resulting energic atoms emit light via fluorescence, with the emitted color characteristic of the contained material.
The Bugs Bunny Crazy Castle is a 1989 puzzle video game developed by Kemco for the Nintendo Entertainment System. In 1990, it was released with the same title for the Game Boy in North America and Europe. In Japan, it was released for the Disk System as Roger Rabbit, and then for the Game Boy as Mickey Mouse.
Pat the Bunny is the first "touch and feel" interactive children's book, written and illustrated by Dorothy Kunhardt. Since its publication in 1940, it has been a perennial best-seller in the United States. Rather than follow a linear narrative, the book invites the reader to engage in tactile activities, such as patting the fake fur of a rabbit, feeling sandpaper that stands for "Daddy's scratchy face," trying on "Mummy's ring," reading a book within a book, playing peekaboo with a cloth, and gazing into a mirror.
The pGLO plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms. The plasmid contains several reporter genes, most notably the green fluorescent protein (GFP) and the ampicillin resistance gene. GFP was isolated from the jelly fish Aequorea victoria. Because it shares a bidirectional promoter with a gene for metabolizing arabinose, the GFP gene is expressed in the presence of arabinose, which makes the transgenic organism express its fluorescence under UV light. GFP can be induced in bacteria containing the pGLO plasmid by growing them on +arabinose plates. pGLO is made by Bio-Rad Laboratories.
Luminous paint is paint that emits visible light through fluorescence, phosphorescence, or radioluminescence.
BioArt is an art practice where artists work with biology, live tissues, bacteria, living organisms, and life processes. Using scientific processes and practices such as biology and life science practices, microscopy, and biotechnology the artworks are produced in laboratories, galleries, or artists' studios. The scope of BioArt is a range considered by some artists to be strictly limited to "living forms", while other artists include art that uses the imagery of contemporary medicine and biological research, or require that it address a controversy or blind spot posed by the very character of the life sciences.
Roger Yonchien Tsien was an American biochemist. He was a professor of chemistry and biochemistry at the University of California, San Diego and was awarded the Nobel Prize in Chemistry in 2008 for his discovery and development of the green fluorescent protein, in collaboration with organic chemist Osamu Shimomura and neurobiologist Martin Chalfie. Tsien was also a pioneer of calcium imaging.
Bioreporters are intact, living microbial cells that have been genetically engineered to produce a measurable signal in response to a specific chemical or physical agent in their environment. Bioreporters contain two essential genetic elements, a promoter gene and a reporter gene. The promoter gene is turned on (transcribed) when the target agent is present in the cell’s environment. The promoter gene in a normal bacterial cell is linked to other genes that are then likewise transcribed and then translated into proteins that help the cell in either combating or adapting to the agent to which it has been exposed. In the case of a bioreporter, these genes, or portions thereof, have been removed and replaced with a reporter gene. Consequently, turning on the promoter gene now causes the reporter gene to be turned on. Activation of the reporter gene leads to production of reporter proteins that ultimately generate some type of a detectable signal. Therefore, the presence of a signal indicates that the bioreporter has sensed a particular target agent in its environment.
Osamu Shimomura was a Japanese organic chemist and marine biologist, and professor emeritus at Marine Biological Laboratory (MBL) in Woods Hole, Massachusetts and Boston University School of Medicine. He was awarded the Nobel Prize in Chemistry in 2008 for the discovery and development of green fluorescent protein (GFP) with two American scientists: Martin Chalfie of Columbia University and Roger Tsien of the University of California-San Diego.
Douglas C. Prasher is an American molecular biologist. He is known for his work to clone and sequence the genes for the photoprotein aequorin and green fluorescent protein (GFP) and for his proposal to use GFP as a tracer molecule. He communicated his pioneering work to Martin Chalfie and Roger Y. Tsien, but by 1991 he was unable to obtain further research funding, and left academia. Eventually, he had to abandon science. Chalfie and Tsien were awarded the 2008 Nobel Prize in Chemistry for work that they publicly acknowledged was substantially based on Prasher's work; through their efforts and those of others, he returned to scientific research in June 2010.
Do-it-yourself biology is a biotechnological social movement in which individuals, communities, and small organizations study biology and life science using the same methods as traditional research institutions. DIY biology is primarily undertaken by individuals with limited research training from academia or corporations, who then mentor and oversee other DIY biologists with little or no formal training. This may be done as a hobby, as a not-for-profit endeavor for community learning and open-science innovation, or for profit, to start a business.
Dedham Pottery was an American art pottery company opened by the Robertson Family in Dedham, Massachusetts during the American arts & crafts movement that operated between 1896 and 1943. It was known for its high-fire stoneware characterized by a controlled and very fine crackle glaze with thick cobalt border designs. The Chelsea Keramic Art Works (1872–1889) and "Chelsea Pottery U.S." were earlier companies of the family.
Bunny Maloney is an animated television series created by Studio Tanuki and directed by Stéphane Stoll. The series is produced by MoonScoop Group, in co-production with France 2 and Telegael. It was based on a Flash-animated pilot called The Attack of the Giant Red Octopus. The series chronicles the adventures of a pink anthropomorphic rabbit named Bunny Maloney and his friends. The series was canceled after one season, due to low ratings and highly negative criticism over the show's inappropriate themes.
References
- 1 2 Philipkoski, Kristen (12 August 2002). "RIP: Alba, the Glowing Bunny". Wired .
- ↑ Cook, Gareth (September 17, 2000). "Cross hare: hop and glow". The Boston Globe . p. A01.
- ↑ Copel, Lib (2000-10-18). "It's Not Easy Being Green". Washington Post. ISSN 0190-8286 . Retrieved 2022-05-12.
- ↑ "Dealing with difficult topics in public: A communication workshop focused on controversial issues in science" (PDF). Archived from the original (PDF) on 2008-07-20.
