Alpha,alpha-trehalase

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alpha,alpha-trehalase
Identifiers
EC number 3.2.1.28
CAS number 9025-52-9
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / QuickGO

In enzymology, an alpha,alpha-trehalase (EC 3.2.1.28) is an enzyme with system name alpha,alpha-trehalose glucohydrolase. [1] [2] [3] [4] This enzyme catalyzes the chemical reaction

alpha,alpha-trehalose + H2O 2 D-glucose

Thus, the two substrates of this enzyme are alpha,alpha-trehalose and H2O, whereas its product is D-glucose.

This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds. The systematic name of this enzyme class is alpha,alpha-trehalose glucohydrolase. This enzyme is also called trehalase. This enzyme participates in starch and sucrose metabolism.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2JF4 and 2JG0.

Related Research Articles

Trehalose

Trehalose is a sugar consisting of two molecules of glucose. It is also known as mycose or tremalose. Some bacteria, fungi, plants and invertebrate animals synthesize it as a source of energy, and to survive freezing and lack of water.

Trehalase

The enzyme Trehalase is a glycoside hydrolase, produced by cells in the brush border of the small intestine, which catalyzes the conversion of trehalose to glucose. It is found in most animals.

Sucrose phosphorylase is an important enzyme in the metabolism of sucrose and regulation of other metabolic intermediates. Sucrose phosphorylase is in the class of hexosyltransferases. More specifically it has been placed in the retaining glycoside hydrolases family although it catalyzes a transglycosidation rather than hydrolysis. Sucrose phosphorylase catalyzes the conversion of sucrose to D-fructose and α-D-glucose-1-phosphate. It has been shown in multiple experiments that the enzyme catalyzes this conversion by a double displacement mechanism.

Glucan 1,4-alpha-glucosidase is an enzyme located on the brush border of the small intestine with systematic name 4-alpha-D-glucan glucohydrolase. This enzyme catalyses the following chemical reaction

In enzymology, a 6-phospho-beta-glucosidase (EC 3.2.1.86) is an enzyme that catalyzes the chemical reaction

In enzymology, an alpha,alpha-phosphotrehalase (EC 3.2.1.93) is an enzyme that catalyzes the chemical reaction

In enzymology, an amygdalin beta-glucosidase (EC 3.2.1.117) is an enzyme that catalyzes the chemical reaction

Glucosylceramidase

In enzymology, a glucosylceramidase (EC 3.2.1.45) is an enzyme that catalyzes the chemical reaction

In enzymology, a protein-glucosylgalactosylhydroxylysine glucosidase (EC 3.2.1.107) is an enzyme that catalyzes the chemical reaction

In enzymology, an alpha,alpha-trehalose phosphorylase (configuration-retaining) is an enzyme that catalyzes the chemical reaction

In enzymology, a kojibiose phosphorylase is an enzyme that catalyzes the chemical reaction

In enzymology, a NDP-glucose—starch glucosyltransferase is an enzyme that catalyzes the chemical reaction

In enzymology, a trehalose 6-phosphate phosphorylase is an enzyme that catalyzes the chemical reaction

Alpha-1,4-glucan-protein synthase (UDP-forming) is an enzyme with systematic name UDP-glucose:protein 4-alpha-glucosyltransferase. This enzyme catalyses the following chemical reaction

Alpha,alpha-trehalose synthase is an enzyme with systematic name ADP-glucose:D-glucose 1-alpha-D-glucosyltransferase. This enzyme catalyses the following chemical reaction

3-O-alpha-D-glucosyl-L-rhamnose phosphorylase is an enzyme with systematic name 3-O-alpha-D-glucopyranosyl-L-rhamnopyranose:phosphate beta-D-glucosyltransferase. This enzyme catalyses the following chemical reaction

Amylo-alpha-1,6-glucosidase is an enzyme with systematic name glycogen phosphorylase-limit dextrin 6-alpha-glucohydrolase. This enzyme catalyses the following chemical reaction

Branched-dextran exo-1,2-alpha-glucosidase is an enzyme with systematic name (1->2)-alpha-D-glucosyl-branched-dextran 2-glucohydrolase. This enzyme catalyses the following chemical reaction

4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase is an enzyme with systematic name 4-alpha-D-( -alpha-D-glucano)trehalose glucanohydrolase (trehalose-producing). This enzyme catalyses the following chemical reaction

(1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase is an enzyme with systematic name (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase. This enzyme catalyses the following chemical reaction

References

  1. ↑ Myrbäck K, Örtenblad B (1937). "Trehalose und Hefe. II. Trehalasewirkung von Hefepräparaten". Biochem. Z. 291: 61–69.
  2. ↑ Kalf GF, Rieder SV (February 1958). "The purification and properties of trehalase". The Journal of Biological Chemistry. 230 (2): 691–8. PMID   13525386.
  3. ↑ Hehre EJ, Sawai T, Brewer CF, Nakano M, Kanda T (June 1982). "Trehalase: stereocomplementary hydrolytic and glucosyl transfer reactions with alpha- and beta-D-glucosyl fluoride". Biochemistry. 21 (13): 3090–7. doi:10.1021/bi00256a009. PMID   7104311.
  4. ↑ Mori H, Lee JH, Okuyama M, Nishimoto M, Ohguchi M, Kim D, Kimura A, Chiba S (November 2009). "Catalytic reaction mechanism based on alpha-secondary deuterium isotope effects in hydrolysis of trehalose by European honeybee trehalase". Bioscience, Biotechnology, and Biochemistry. 73 (11): 2466–73. doi:10.1271/bbb.90447. PMID   19897915. S2CID   22774772.


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