Dioscoreophyllum volkensii

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Dioscoreophyllum volkensii
Scientific classification OOjs UI icon edit-ltr.svg
Kingdom: Plantae
Clade: Tracheophytes
Clade: Angiosperms
Clade: Eudicots
Order: Ranunculales
Family: Menispermaceae
Genus: Dioscoreophyllum
Species:
D. volkensii
Binomial name
Dioscoreophyllum volkensii
Synonyms [1]
  • Dioscoreophyllum fernandenseHutch. & Dalziel
  • Dioscoreophyllum podandriumExell
  • Dioscoreophyllum tenerumEngl.

Dioscoreophyllum volkensii, the serendipity berry, is a tropical dioecious rainforest vine in the family Menispermaceae. It is native throughout most of tropical Africa from Sierra Leone east to Eritrea, and south to Angola and Mozambique. It grows at low altitudes, from sea level up to 400 m. Some authors separate plants from above 200 m as a separate species D. cumminsii (Stapf) Diels. [2]

There are two varieties: [2]

Uses

The fruit contains monellin, an intensely sweet protein with potential use as a sugar replacement. For humans, monellin is 100,000 times sweeter than sucrose on a molar basis and around 3,000 times on a weight basis. [3] [4]

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Volkensin is a eukaryotic ribosome-inactivating protein found in the Adenia volkensii plant. It is a glycoprotein with two subunits A and B. A subunit is linked to B subunit with disulfide bridges and non-covalent bonds. B subunit is responsible for binding to the galactosyl-terminated receptors on the cell membrane that allows the entry the A subunit of the toxin into the cell, which performs the inhibitory function. Volkensin is a galactose specific lectin that can inhibit protein synthesis in whole cells and in cell-free lysates. This protein can be included into the category of risin like toxins and it resembles modeccin, the toxin of Adenia digitata. Although very similar in composition, volkensin contains more cysteine residues and more than twice as much sugar than modeccin, due to high content of galactose and mannose. In addition, volkensin is able to inhibit protein synthesis at concentrations 10 times lower than required for modeccin. From gene sequencing analysis, volkensin was found to be coded by 1569-bp ORF, that is 523 amino acid residues without introns. The internal linker sequence is 45 bp. The active site of the A subunit contains Ser203, a novel residue that is conserved in all ribosome inactivating proteins.

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References

  1. The Plant List
  2. 1 2 African Plants Database: Dioscoreophyllum volkensii Archived 2007-10-08 at the Wayback Machine
  3. This, Hervé (2006). Molecular Gastronomy: Exploring the Science of Flavor . Columbia University Press. ISBN   978-0-231-13312-8.
  4. Kant, Ravi (2005). "Sweet proteins – Potential replacement for artificial low calorie sweeteners". Nutrition Journal. BioMed Central. 4: 5. doi: 10.1186/1475-2891-4-5 . PMC   549512 . PMID   15703077.