Galactan endo-1,6-beta-galactosidase

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Galactan endo-1,6-beta-galactosidase
Identifiers
EC no. 3.2.1.164
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BRENDA BRENDA entry
ExPASy NiceZyme view
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MetaCyc metabolic pathway
PRIAM profile
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NCBI proteins

Galactan endo-1,6-beta-galactosidase (EC 3.2.1.164, endo-1,6-beta-galactanase) is an enzyme with systematic name endo-beta-(1->6)-galactanase. [1] [2] [3] This enzyme catalyses the following chemical reaction

Endohydrolysis of (1->6)-beta-D-galactosidic linkages in arabinogalactan proteins and (1->3):(1->6)-beta-galactans to yield galactose and (1->6)-beta-galactobiose as the final products

The enzyme specifically hydrolyses 1,6-beta-D-galactooligosaccharides with a degree of polymerization (DP) higher than 3.

References

  1. Brillouet, J.-M.; Williams, P.; Moutounet, M. (1991). "Purification and some properties of a novel endo-β-(1→6)-D-galactanase from Aspergillus niger". Agric. Biol. Chem. 55 (6): 1565–1571. doi: 10.1271/bbb1961.55.1565 .
  2. Okemoto K, Uekita T, Tsumuraya Y, Hashimoto Y, Kasama T (January 2003). "Purification and characterization of an endo-beta-(1-->6)-galactanase from Trichoderma viride". Carbohydrate Research. 338 (3): 219–30. doi:10.1016/s0008-6215(02)00405-6. PMID   12543554.
  3. Kotake T, Kaneko S, Kubomoto A, Haque MA, Kobayashi H, Tsumuraya Y (February 2004). "Molecular cloning and expression in Escherichia coli of a Trichoderma viride endo-beta-(1-->6)-galactanase gene". The Biochemical Journal. 377 (Pt 3): 749–55. doi:10.1042/BJ20031145. PMC   1223900 . PMID   14565843.