Galactan endo-beta-1,3-galactanase

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Galactan endo-beta-1,3-galactanase
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EC no. 3.2.1.181
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Galactan endo-beta-1,3-galactanase (EC 3.2.1.181, endo-beta-1,3-galactanase) is an enzyme with systematic name arabinogalactan 3-beta-D-galactanohydrolase. [1] This enzyme catalyses the following chemical reaction

The enzyme specifically hydrolyses beta-1,3-galactan and beta-1,3-galactooligosaccharides

The enzyme from the fungus Flammulina velutipes (winter mushroom) hydrolyses the beta(1->3) bonds found in type II plant arabinogalactans.

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endo-1,3-β-Xylanase, EC 3.2.1.32 [xylanase (ambiguous), endo-1,3-β-xylosidase, 1,3-β-xylanase, 1,3-xylanase, β-1,3-xylanase, endo-β-1,3-xylanase, 1,3-β-D-xylan xylanohydrolase, xylan endo-1,3-β-xylosidase (misleading)] is an enzyme with systematic name 3-β-D-xylan xylanohydrolase. It catalyses the following chemical reaction:

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Blood-group-substance endo-1,4-β-galactosidase is an enzyme with systematic name blood-group-substance 4-β-D-galactanohydrolase. It catalyses endohydrolysis of (1→4)-β-D-galactosidic linkages in blood group A and B substances.

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Yariv reagent is a glycosylated phenolic compound that binds strongly to galactans and arabinogalactan proteins. It can therefore be used in their detection, quantification, precipitation, isolation, staining, and interfere with their function. It was initially synthesised in 1962 as an antigen for carbohydrate-binding antibodies but has subsequently become more broadly used. There are many variants of Yariv reagents which vary in the glycosyl groups on the outside of the structure, typically glucose, galactose, and mannose.

References

  1. Kotake T, Hirata N, Degi Y, Ishiguro M, Kitazawa K, Takata R, Ichinose H, Kaneko S, Igarashi K, Samejima M, Tsumuraya Y (August 2011). "Endo-beta-1,3-galactanase from winter mushroom Flammulina velutipes". The Journal of Biological Chemistry. 286 (31): 27848–54. doi: 10.1074/jbc.m111.251736 . PMC   3149374 . PMID   21653698.