Glycoside hydrolase family 4

Family 4 glycosyl hydrolase
Structure of the 6-phospho-beta glucosidase from Thermotoga maritima at 2.55 angstrom resolution in the tetragonal form with manganese, NAD+ and glucose-6-phosphate
Identifiers
Symbol	Glyco_hydro_4
Pfam	PF02056
Pfam clan	CL0063
InterPro	IPR001088
PROSITE	PDOC01027
SCOP2	1obb / SCOPe / SUPFAM
CAZy	GH4
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Family 4 glycosyl hydrolase C-terminal domain
Identifiers
Symbol	Glyco_hydro_4C
Pfam	PF11975
Pfam clan	CL0341
PROSITE	PDOC01027
SCOP2	1obb / SCOPe / SUPFAM
CAZy	GH4
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

In molecular biology, glycoside hydrolase family 4 is a family of glycoside hydrolases EC 3.2.1., which are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families. ^{[1] [2] [3]} This classification is available on the CAZy web site, ^{[4] [5]} and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes. ^{[6] [7]}

Glycoside hydrolase family 4 CAZY GH_4 comprises enzymes with several known activities; 6-phospho-beta-glucosidase (EC 3.2.1.86); 6-phospho-alpha-glucosidase (EC 3.2.1.122); alpha-galactosidase (EC 3.2.1.22); alpha-D-glucuronidase (EC 3.2.1.139). ^[8] 6-phospho-alpha-glucosidase requires both NAD(H) and divalent metal (Mn²⁺, Fe²⁺, Co²⁺, or Ni²⁺) for activity. ^[9]

External references

GH4 in CAZypedia

References

1. Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G (July 1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proceedings of the National Academy of Sciences of the United States of America. 92 (15): 7090–4. Bibcode:1995PNAS...92.7090H. doi: 10.1073/pnas.92.15.7090 . PMC   41477 . PMID   7624375.
2. Davies G, Henrissat B (September 1995). "Structures and mechanisms of glycosyl hydrolases". Structure. 3 (9): 853–9. doi: 10.1016/S0969-2126(01)00220-9 . PMID   8535779.
3. Henrissat B, Bairoch A (June 1996). "Updating the sequence-based classification of glycosyl hydrolases". The Biochemical Journal. 316 (Pt 2): 695–6. doi:10.1042/bj3160695. PMC   1217404 . PMID   8687420.
4. "Home". CAZy.org. Retrieved 2018-03-06.
5. Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B (January 2014). "The carbohydrate-active enzymes database (CAZy) in 2013". Nucleic Acids Research. 42 (Database issue): D490-5. doi:10.1093/nar/gkt1178. PMC   3965031 . PMID   24270786.
6. "Glycoside Hydrolase Family 4". CAZypedia.org. Retrieved 2018-03-06.
7. CAZypedia Consortium (December 2018). "Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes" (PDF). Glycobiology. 28 (1): 3–8. doi: 10.1093/glycob/cwx089 . PMID   29040563.
8. Mohapatra, Samar Ballabha; Manoj, Narayanan (2021-02-26). "Structural basis of catalysis and substrate recognition by the NAD(H)-dependent α-d-glucuronidase from the glycoside hydrolase family 4". Biochemical Journal. 478 (4): 943–959. doi:10.1042/BCJ20200824. ISSN   0264-6021.
9. Thompson J, Pikis A, Ruvinov SB, Henrissat B, Yamamoto H, Sekiguchi J (October 1998). "The gene glvA of Bacillus subtilis 168 encodes a metal-requiring, NAD(H)-dependent 6-phospho-alpha-glucosidase. Assignment to family 4 of the glycosylhydrolase superfamily". The Journal of Biological Chemistry. 273 (42): 27347–56. doi: 10.1074/jbc.273.42.27347 . PMID   9765262.

This article incorporates text from the public domain Pfam and InterPro: IPR001088