The following are the national records in track cycling in Czech Republic maintained by the Czech Cycling Federation.
Key to tables: Awaiting ratification Record not kept by Czech Cycling Federation Not ratified or later rescinded by the Czech Cycling Federation
| Event | Record | Athlete | Date | Meet | Place | Ref |
|---|---|---|---|---|---|---|
| Flying 200 m time trial | 9.533 | Martin Čechman | 16 March 2025 | Nations Cup | Konya, Turkey | [1] |
| 250 m time trial (standing start) | 17.409 | Matěj Tamme | 12 February 2025 | European Championships | Heusden-Zolder, Belgium | [2] |
| Flying 500 m time trial | 26.914 | Tomáš Bábek | 13 October 2009 | Vienna, Austria | ||
| Team sprint (750 m) | 42.646 | Matěj Tamme Martin Čechman David Peterka | 14 March 2025 | Nations Cup | Konya, Turkey | [3] |
| 1 km time trial | 59.846 | Robin Wagner | 7 December 2013 | World Cup | Aguascalientes, Mexico | [4] |
| 1 km time trial (sea level) | 59.870 | David Peterka | 24 October 2025 | World Championships | Santiago, Chile | [5] |
| 4000m individual pursuit | 4:26.270 | Nicolas Pietrula | 12 July 2016 | Montichiari, Italy | ||
| 4:24.388 | Nicolas Pietrula | 21 October 2017 | European Championships | Berlin, Germany | [6] | |
| 4000m team pursuit | 4:08.224 | Ondřej Rybín Denis Rugovac František Sisr Ondřej Vendolský | 10 July 2013 | European U23 Championships | Anadia, Portugal | |
| Hour record | 49.700 km | Ondřej Sosenka | 19 July 2005 | Moscow, Russia |