Protein-glucosylgalactosylhydroxylysine glucosidase

protein-glucosylgalactosylhydroxylysine glucosidase
Identifiers
EC no.	3.2.1.107
CAS no.	72829-45-9
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

The enzyme protein-glucosylgalactosylhydroxylysine glucosidase (EC 3.2.1.107) catalyzes the following chemical reaction:

[collagen]-(5R)-5-O-[α-D-glucosyl-(1→2)-β-D-galactosyl]-5-hydroxy-L-lysine + H₂O = D-glucose + [collagen]-(5R)-5-O-(β-D-galactosyl)-5-hydroxy-L-lysine

It belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds. The systematic name is protein-α-D-glucosyl-1,2-β-D-galactosyl-L-hydroxylysine glucohydrolase. Other names in common use include 2-O-α-D-glucopyranosyl-5-O-α-D-galactopyranosylhydroxy-L-lysine glucohydrolase, and lysine glucohydrolase.

References

• Hamazaki H, Hotta K (1979). "Purification and characterization of an α-glucosidase specific for hydroxylysine-linked disaccharide of collagen". J. Biol. Chem. 254 (19): 9682–7. doi: 10.1016/S0021-9258(19)83570-6 . PMID   385589.
• Hamazaki H, Hotta K (1980). "Enzymatic hydrolysis of disaccharide unit of collagen. Isolation of 2-O-α-D-glucopyranosyl-O-β-D-galactopyranosyl-hydroxylysine glucohydrolase from rat spleens". Eur. J. Biochem. 111 (2): 587–91. doi: 10.1111/j.1432-1033.1980.tb04975.x . PMID   7460918.
• Sternberg M, Spiro RG (1979). "Studies on the catabolism of the hydroxylysine-linked disaccharide units of basement membranes and collagens. Isolation and characterization of a rat kidney α-glucosidase of high specificity". J. Biol. Chem. 254 (20): 10329–36. doi: 10.1016/S0021-9258(19)86713-3 . PMID   385599.