TEDC2

Last updated
TEDC2
Identifiers
Aliases TEDC2 , C16orf59, chromosome 16 open reading frame 59, tubulin epsilon and delta complex 2
External IDs MGI: 1919266 HomoloGene: 45943 GeneCards: TEDC2
Orthologs
SpeciesHumanMouse
Entrez

80178

72016

Ensembl

ENSG00000162062

ENSMUSG00000024118

UniProt

Q7L2K0

Q6GQV0

RefSeq (mRNA)

NM_025108

NM_028056

RefSeq (protein)

NP_079384

NP_082332

Location (UCSC) Chr 16: 2.46 – 2.46 Mb Chr 17: 24.43 – 24.44 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Tubulin epsilon and delta complex 2 (TEDC2), also known as Chromosome 16 open reading frame 59 (C16orf59), is a protein that in humans is encoded by the TEDC2 gene. Its NCBI accession number is NP_079384.2. [5]

Contents

Gene

A diagram from NCBI showing TEDC2 and its gene neighborhood on chromosome 16. NCBI gene neighborhood TEDC2.gif
A diagram from NCBI showing TEDC2 and its gene neighborhood on chromosome 16.

Locus

TEDC2 is found on chromosome 16 at location 16p13.3, or chr16:2,460,080-2,464,963 (spanning 4883 bp) on the plus strand. [6]

Homology and Evolution

Orthologs

TEDC2 appeared between 684-797 million years ago. Its most distant ortholog is found in Branchiostoma floridae, the Florida lancelet, [7] which diverged from other chordates around 684 million years ago. [8] However, the gene arose more recently than 797 million years ago, when protostomes and deuterostomes diverged, [8] as it is not found in any invertebrates. A table showing 20 selected orthologs is below, found with NCBI BLAST. [5]

Genus and speciesCommon nameCladeDate of divergence (estimated) [8] Accession numberLengthIdentitySimilarity
Homo sapiensHuman Mammalia 0 MYANP_079384.2433 aa100%100%
Pan troglodytesChimpanzeeMammalia6.65 MYAXP_001163226.2433 aa98%98%
Mus musculusHouse mouseMammalia90 MYANP_082332.1436 aa62%72%
Orcinus orcaOrcaMammalia96 MYAXP_012388677.1445 aa71%78%
Vulpes vulpesRed foxMammalia96 MYAXP_025840713.1432 aa68%74%
Dasypus novemcinctusNine-banded armadilloMammalia105 MYAXP_012385078.1469 aa69%77%
Cyanistes caeruleusEurasian blue tit Aves 312 MYAXP_023792200.1366 aa44%59%
Pygoscelis adeliaeAdélie penguinAves312 MYAXP_009325519.1490 aa43%58%
Columba liviaRock doveAves312 MYAXP_021147488.1511 aa43%56%
Numida meleagrisHelmeted guineafowlAves312 MYAXP_021267460.1574 aa40%67%
Dromaius novaehollandiaeEmuAves312 MYAXP_025956253.1547 aa40%68%
Anolis carolinensisGreen anole Reptilia 312 MYAXP_008122311.2473 aa32%47%
Python bivittatusBurmese pythonReptilia312 MYAXP_007433089.1607 aa32%50%
Pogona vitticepsBearded dragonReptilia312 MYAXP_020663843.1578 aa31%45%
Xenopus tropicalisWestern clawed frog Amphibia 352 MYAXP_002932464.1452 aa30%45%
Lepisosteus oculatusSpotted gar Osteichthyes 435 MYAXP_015215377.1193 aa37%48%
Scleropages formosusAsian arowanaOsteichthyes435 MYAXP_018598511.1186 aa29%47%
Paramormyrops kingsleyaeElephantfishOsteichthyes435 MYAXP_023666461.1473 aa29%46%
Callorhinchus miliiAustralian ghostshark Chondrichthyes 473 MYAXP_007891790.1540 aa32%49%
Branchiostoma floridaeFlorida lancelet Cephalochordata 684 MYAXP_002611730.1602 aa23%42%

Paralogs

There are no other members of the TEDC2 gene family, as it has no paralogs in any living organisms. [5]

Expression

Transcription Factors

Conserved predicted transcription factor binding sites found in the 5' region upstream of TEDC2 are WT1, ZKSCAN3 (x2), AREB6, MZF1 (x2), ATF6, ER, and P53. [9] This suggests that these transcription factors in particular, and especially ZKSCAN3 and MZF1 on the basis of multiple conserved binding sites, are crucial in the regulation of TEDC2. ZKSCAN3 is a transcriptional repressor of autophagy, [10] and MZF1 is thought to play a role as a tumor suppressor and regulator of cell proliferation. [11] These conserved MZF1 sites, along with the conserved p53 site, suggest that TEDC2 could play a role in cell proliferation and can therefore impact the genesis and development of cancer.

Localization

TEDC2 is predicted to be localized to the nucleus and may also be present in the cytoplasm, mitochondria, peroxisomes, and extracellular space. [12]

Expression

It is highly expressed in the testis and EBV-transformed lymphocytes. [13] It is also highly expressed in lymph node, fetal liver, early erythroid cell, and B-lymphoblasts. [14] It is also seen at higher levels in both embryonic stem cells and induced pluripotent stem cells than fibroblasts. [14] Finally, relative to other genes, TEDC2 expression significantly decreases in breast cancer cells upon estrogen starvation. [14]

Transcript Variants

A table showing which exons are found in the 6 TEDC2 isoforms. Green = contains a start codon. Red = contains a stop codon. Check = Exon included in the final mRNA transcript. -- = exon is excluded from the final mRNA transcript. U = exon is present but is untranslated. Isoformtabletedc2.png
A table showing which exons are found in the 6 TEDC2 isoforms. Green = contains a start codon. Red = contains a stop codon. Check = Exon included in the final mRNA transcript. -- = exon is excluded from the final mRNA transcript. U = exon is present but is untranslated.

The gene has 10 exons. [15] The gene has 13 alternatively spliced transcripts, with 6 coding for a protein, 1 undergoing nonsense-mediated decay, and 6 being retained introns. [16]

Protein

General Features

TEDC2 is encoded by the TEDC2 gene with NCBI accession number NM_025108.3. The protein is 433 amino acids long with a predicted molecular weight of 46.4 kDa. [6] There is an antibody against the protein, but a sample western blot image is not available. [17]

Domains

TEDC2 contains a domain of unknown function, DUF4693, which in humans spans from proteins 148-431, approximately the last two-thirds of the protein. [5]

Secondary Structure

Using online bioinformatics tools, TEDC2 is predicted to have many alpha helices, and it has two well-conserved predicted beta-pleated sheets near the end of the protein. [18] [19]

Tertiary Structure

The predicted TEDC2 tertiary structure model of highest probability, generated by I-TASSER. TEDC2 I-TASSER PredictedStructure.png
The predicted TEDC2 tertiary structure model of highest probability, generated by I-TASSER.

TEDC2 is predicted to form tertiary structure based on its alpha helices. Many of these predicted alpha helices are highly conserved in orthologs, and one example of predicted tertiary structure generated by I-TASSER is shown to the right. [20]

Post-translational Modifications

Graphical representation of TEDC2 showing domains and modification sites, created with DOG. TEDC2 domains DOG.png
Graphical representation of TEDC2 showing domains and modification sites, created with DOG.

TEDC2 has a well conserved predicted O-GlcNAc site at S114 in humans. [22] O-GlcNAcylated proteins are found mostly in the nucleus, sometimes also being found in the cytoplasm, and this is a dynamic modification, frequently being removed and reattached. [23]

TEDC2 also has three conserved, predicted C-mannosylation sites. [24] The function of C-mannosylation is still unclear, but it is the attachment of an alpha-mannose to a tryptophan. [25]

TEDC2 also has many possible phosphorylation sites, including seven that are well-conserved. [26] Phosphorylation is an important means of protein regulation, activation, and inactivation, so it is difficult to determine any specific function from the presence of a serine or threonine that could be phosphorylated. [27]

Interactions

Protein-Protein Interactions

KDM1A, a lysine-specific demethylase, was shown to be physically associated with TEDC2. [28] [29] TEDC2 also interacts with FEZ1, a fasciculation and elongation protein. FEZ1, or fasciculation and elongation protein 1, is necessary for axon growth but is also thought to be involved in transcriptional control. [30]

There is also experimental evidence for TEDC2 interaction with TUBE1 and C14orf80. TUBE1, or Tubulin epsilon 1, is involved with the centrioles during cell division, and the function of C14orf80 is unknown. [31] TEDC2 is also co-expressed with CDC45, or cell division control protein 45, which is required for initiation of chromosomal DNA replication, as well as co-expression with CDT1, a DNA replication licensing factor required for pre-replication assembly. [32]

Function and Clinical Significance

The function of TEDC2 is not yet known with certainty by the scientific community, but its expression profile, predicted transcription factor binding sites, and other protein-protein interactions enable some predictions. TEDC2 is localized in the nucleus and is often expressed in developing tissues such as stem cells as well as differentiated fetal tissue, so it likely plays a role in DNA replication and/or cell division. [12] [14] This also fits with TEDC2's predicted or known protein-protein interactions, as it may interact with proteins involved in cell division (TUBE1, CDC45, CDT1), as well as remain under transcriptional control of tumor suppressors (WT1, MZF1, P53). [9] Additionally, given the presence of an estrogen-response element binding-site, it is possible that TEDC2 plays a role in tumor development when mutated. [9]

Related Research Articles

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Uncharacterized protein C16orf86 is a protein in humans that is encoded by the C16orf86 gene. It is mostly made of alpha helices and it is expressed in the testes, but also in other tissues such as the kidney, colon, brain, fat, spleen, and liver. For the function of C16orf86, it is not well understood, however it could be a transcription factor in the nucleus that regulates G0/G1 in the cell cycle for tissues such as the kidney, brain, and skeletal muscles as mentioned in the DNA microarray data below in the gene level regulation section.

<span class="mw-page-title-main">CFAP299</span> Protein-coding gene in the species Homo sapiens

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References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000162062 - Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000024118 - Ensembl, May 2017
  3. ↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. 1 2 3 4 "tubulin epsilon and delta complex protein 2 [Homo sapiens] - Protein - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2019-03-03.
  6. 1 2 www.genecards.org https://www.genecards.org/cgi-bin/carddisp.pl?gene=TEDC2 . Retrieved 2019-02-08.
  7. ↑ "hypothetical protein BRAFLDRAFT_128731 [Branchiostoma floridae] - Protein - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2019-02-25.
  8. 1 2 3 "TimeTree :: The Timescale of Life". www.timetree.org. Retrieved 2019-03-03.
  9. 1 2 3 "Genomatix: MatInspector Input". www.genomatix.de. Retrieved 2019-05-05.
  10. ↑ Chauhan S, Goodwin JG, Chauhan S, Manyam G, Wang J, Kamat AM, Boyd DD (April 2013). "ZKSCAN3 is a master transcriptional repressor of autophagy". Molecular Cell. 50 (1): 16–28. doi:10.1016/j.molcel.2013.01.024. PMC   3628091 . PMID   23434374.
  11. ↑ Gaboli M, Kotsi PA, Gurrieri C, Cattoretti G, Ronchetti S, Cordon-Cardo C, Broxmeyer HE, Hromas R, Pandolfi PP (July 2001). "Mzf1 controls cell proliferation and tumorigenesis". Genes & Development. 15 (13): 1625–30. doi:10.1101/gad.902301. PMC   312729 . PMID   11445537.
  12. 1 2 "COMPARTMENTS - C16orf59". compartments.jensenlab.org. Retrieved 2019-02-08.
  13. ↑ "GTEx Portal - C16orf59". GTEx. Retrieved 2/8/19.
  14. 1 2 3 4 "Home - GEO - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2019-04-22.
  15. ↑ "Homo sapiens tubulin epsilon and delta complex 2 (TEDC2), mRNA". 2018-12-29.{{cite journal}}: Cite journal requires |journal= (help)
  16. ↑ "Gene: TEDC2 (ENSG00000162062) - Summary - Homo sapiens - Ensembl genome browser 95". useast.ensembl.org. Retrieved 2019-02-25.
  17. ↑ "Anti-C16ORF59 antibody produced in rabbit HPA051394". Sigma-Aldrich. Retrieved 2019-05-05.
  18. ↑ "CFSSP: Chou & Fasman Secondary Structure Prediction Server". www.biogem.org. Retrieved 2019-04-22.
  19. ↑ "JPred: A Protein Secondary Structure Prediction Server". www.compbio.dundee.ac.uk. Retrieved 2019-04-22.
  20. 1 2 "I-TASSER server for protein structure and function prediction". zhanglab.ccmb.med.umich.edu. Retrieved 2019-05-05.
  21. ↑ "DOG 2.0 - Protein Domain Structure Visualization". dog.biocuckoo.org. Retrieved 2019-05-05.
  22. ↑ "YinOYang 1.2 Server". www.cbs.dtu.dk. Retrieved 2019-04-22.
  23. ↑ Varki A, Cummings RD, Esko JD, Stanley P, Hart GW, Aebi M, et al. (2015). "The O-GlcNAc Modification". In Varki A, Cummings RD, Esko JD, Stanley P (eds.). Essentials of Glycobiology (3rd ed.). Cold Spring Harbor Laboratory Press. doi:10.1101/glycobiology.3e.019 (inactive 31 January 2024). PMID   28876858 . Retrieved 2019-04-22.{{cite book}}: CS1 maint: DOI inactive as of January 2024 (link)
  24. ↑ "NetCGlyc 1.0 Server". www.cbs.dtu.dk. Retrieved 2019-04-22.
  25. ↑ Ihara Y, Inai Y, Ikezaki M, Matsui IS, Manabe S, Ito Y (2015). "C-mannosylation: modification on tryptophan in cellular proteins". Glycoscience: biology and medicine. pp. 1091–9. doi:10.1007/978-4-431-54836-2_67-1. ISBN   9784431548362. S2CID   82050024.
  26. ↑ "NetPhos 3.1 Server". www.cbs.dtu.dk. Retrieved 2019-04-22.
  27. ↑ Greengard, Paul; Nestler, Eric J. (1999). "Protein Phosphorylation is of Fundamental Importance in Biological Regulation". Basic Neurochemistry: Molecular, Cellular and Medical Aspects (6th ed.).
  28. ↑ "The Molecular INTeraction Database – An ELIXIR Core Resource" . Retrieved 2019-04-22.
  29. ↑ "mentha: the interactome browser". www.mentha.uniroma2.it. Retrieved 2019-04-22.
  30. ↑ Assmann EM, Alborghetti MR, Camargo ME, Kobarg J (April 2006). "FEZ1 dimerization and interaction with transcription regulatory proteins involves its coiled-coil region". The Journal of Biological Chemistry. 281 (15): 9869–81. doi: 10.1074/jbc.M513280200 . PMID   16484223.
  31. ↑ Breslow DK, Hoogendoorn S, Kopp AR, Morgens DW, Vu BK, Kennedy MC, Han K, Li A, Hess GT, Bassik MC, Chen JK, Nachury MV (March 2018). "A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies". Nature Genetics. 50 (3): 460–471. doi:10.1038/s41588-018-0054-7. PMC   5862771 . PMID   29459677.
  32. ↑ "STRING: functional protein association networks". string-db.org. Retrieved 2019-04-22.
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