TRNA (carboxymethyluridine34-5-O)-methyltransferase

TRNA (carboxymethyluridine34-5-O)-methyltransferase
Identifiers
EC no.	2.1.1.229
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

TRNA (carboxymethyluridine³⁴-5-O)-methyltransferase (EC 2.1.1.229, ALKBH8, ABH8, Trm9, tRNA methyltransferase 9) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (carboxymethyluridine³⁴-5-O)-methyltransferase. ^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + carboxymethyluridine ³⁴ in tRNA ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine³⁴ in tRNA

The enzyme catalyses the posttranslational modification of uridine residues at the wobble position 34 of the anticodon loop of tRNA.

References

1. Fu D, Brophy JA, Chan CT, Atmore KA, Begley U, Paules RS, Dedon PC, Begley TJ, Samson LD (May 2010). "Human AlkB homolog ABH8 Is a tRNA methyltransferase required for wobble uridine modification and DNA damage survival". Molecular and Cellular Biology. 30 (10): 2449–59. doi:10.1128/MCB.01604-09. PMC   2863699 . PMID   20308323.
2. Songe-Møller L, van den Born E, Leihne V, Vågbø CB, Kristoffersen T, Krokan HE, Kirpekar F, Falnes PØ, Klungland A (April 2010). "Mammalian ALKBH8 possesses tRNA methyltransferase activity required for the biogenesis of multiple wobble uridine modifications implicated in translational decoding". Molecular and Cellular Biology. 30 (7): 1814–27. doi:10.1128/MCB.01602-09. PMC   2838068 . PMID   20123966.
3. Kalhor HR, Clarke S (December 2003). "Novel methyltransferase for modified uridine residues at the wobble position of tRNA". Molecular and Cellular Biology. 23 (24): 9283–92. doi:10.1128/mcb.23.24.9283-9292.2003. PMC   309612 . PMID   14645538.

External links

• TRNA+(carboxymethyluridine34-5-O)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)