TRNA pseudouridine32 synthase

tRNA pseudouridine32 synthase
Identifiers
EC no.	5.4.99.28
CAS no.	430429-15-5
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

tRNA pseudouridine³² synthase (EC 5.4.99.28, RluA, pseudouridine synthase RluA, Pus9p, Rib2/Pus8p) is an enzyme with systematic name tRNA-uridine³² uracil mutase. ^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction

tRNA uridine³²${\displaystyle \rightleftharpoons }$ tRNA pseudouridine³²

The dual enzyme from Escherichia coli also catalyses the formation of pseudouridine⁷⁴⁶ in 23S rRNA.

References

1. Hoang C, Chen J, Vizthum CA, Kandel JM, Hamilton CS, Mueller EG, Ferré-D'Amaré AR (November 2006). "Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure". Molecular Cell. 24 (4): 535–45. doi: 10.1016/j.molcel.2006.09.017 . PMID   17188032.
2. Spedaliere CJ, Hamilton CS, Mueller EG (August 2000). "Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues". Biochemistry. 39 (31): 9459–65. doi:10.1021/bi001079n. PMID   10924141.
3. Raychaudhuri S, Niu L, Conrad J, Lane BG, Ofengand J (July 1999). "Functional effect of deletion and mutation of the Escherichia coli ribosomal RNA and tRNA pseudouridine synthase RluA". The Journal of Biological Chemistry. 274 (27): 18880–6. doi: 10.1074/jbc.274.27.18880 . PMID   10383384.
4. Ramamurthy V, Swann SL, Spedaliere CJ, Mueller EG (October 1999). "Role of cysteine residues in pseudouridine synthases of different families". Biochemistry. 38 (40): 13106–11. doi:10.1021/bi9913911. PMID   10529181.
5. Wrzesinski J, Nurse K, Bakin A, Lane BG, Ofengand J (June 1995). "A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for psi 746 in 23S RNA is also specific for psi 32 in tRNA(phe)". RNA. 1 (4): 437–48. PMC   1482406 . PMID   7493321.
6. Behm-Ansmant I, Grosjean H, Massenet S, Motorin Y, Branlant C (December 2004). "Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae" (PDF). The Journal of Biological Chemistry. 279 (51): 52998–3006. doi: 10.1074/jbc.m409581200 . PMID   15466869. S2CID   13197875.

External links

• TRNA+pseudouridine32+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)