UDP-N-acetylglucosamine 2-epimerase (hydrolysing)

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UDP-N-acetylglucosamine 2-epimerase (hydrolysing)
Identifiers
EC no. 3.2.1.183
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UDP-N-acetylglucosamine 2-epimerase (hydrolysing) (EC 3.2.1.183, UDP-N-acetylglucosamine 2-epimerase, GNE (gene), siaA (gene), neuC (gene)) is an enzyme with systematic name UDP-N-acetyl-alpha-D-glucosamine hydrolase (2-epimerising). [1] [2] [3] [4] This enzyme catalyses the following chemical reaction

UDP-N-acetyl-alpha-D-glucosamine + H2O N-acetyl-D-mannosamine + UDP

The enzyme is found in mammalian liver, as well as in some pathogenic bacteria including Neisseria meningitidis and Staphylococcus aureus .

References

  1. ↑ Stäsche R, Hinderlich S, Weise C, Effertz K, Lucka L, Moormann P, Reutter W (September 1997). "A bifunctional enzyme catalyzes the first two steps in N-acetylneuraminic acid biosynthesis of rat liver. Molecular cloning and functional expression of UDP-N-acetyl-glucosamine 2-epimerase/N-acetylmannosamine kinase". The Journal of Biological Chemistry. 272 (39): 24319–24. doi: 10.1074/jbc.272.39.24319 . PMID   9305888.
  2. ↑ Chou WK, Hinderlich S, Reutter W, Tanner ME (March 2003). "Sialic acid biosynthesis: stereochemistry and mechanism of the reaction catalyzed by the mammalian UDP-N-acetylglucosamine 2-epimerase". Journal of the American Chemical Society. 125 (9): 2455–61. Bibcode:2003JAChS.125.2455C. doi:10.1021/ja021309g. PMID   12603133.
  3. ↑ Blume A, Ghaderi D, Liebich V, Hinderlich S, Donner P, Reutter W, Lucka L (June 2004). "UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, functionally expressed in and purified from Escherichia coli, yeast, and insect cells". Protein Expression and Purification. 35 (2): 387–96. doi:10.1016/j.pep.2004.02.013. PMID   15135418.
  4. ↑ Murkin AS, Chou WK, Wakarchuk WW, Tanner ME (November 2004). "Identification and mechanism of a bacterial hydrolyzing UDP-N-acetylglucosamine 2-epimerase". Biochemistry. 43 (44): 14290–8. doi:10.1021/bi048606d. PMID   15518580.
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