23S rRNA (guanine748-N1)-methyltransferase

23S rRNA (guanine748-N1)-methyltransferase
Identifiers
EC no.	2.1.1.188
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

23S rRNA (guanine⁷⁴⁸-N¹)-methyltransferase (EC 2.1.1.188, Rlma(II), Rlma2, 23S rRNA m1G748 methyltransferase, RlmaII, Rlma II, tylosin-resistance methyltransferase RlmA(II), TlrB, rRNA large subunit methyltransferase II) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (guanine⁷⁴⁸-N¹)-methyltransferase. ^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + guanine ⁷⁴⁸ in 23S rRNA ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + N¹-methylguanine ⁷⁴⁸ in 23S rRNA

The enzyme specifically methylates guanine⁷⁴⁸ at N¹ in 23S rRNA.

References

1. Douthwaite S, Crain PF, Liu M, Poehlsgaard J (April 2004). "The tylosin-resistance methyltransferase RlmA(II) (TlrB) modifies the N-1 position of 23S rRNA nucleotide G748". Journal of Molecular Biology. 337 (5): 1073–7. doi:10.1016/j.jmb.2004.02.030. PMID   15046978.
2. Liu M, Kirpekar F, Van Wezel GP, Douthwaite S (August 2000). "The tylosin resistance gene tlrB of Streptomyces fradiae encodes a methyltransferase that targets G748 in 23S rRNA". Molecular Microbiology. 37 (4): 811–20. doi:10.1046/j.1365-2958.2000.02046.x. hdl: 1887/3221371 . PMID   10972803.
3. Lebars I, Yoshizawa S, Stenholm AR, Guittet E, Douthwaite S, Fourmy D (January 2003). "Structure of 23S rRNA hairpin 35 and its interaction with the tylosin-resistance methyltransferase RlmAII". The EMBO Journal. 22 (2): 183–92. doi:10.1093/emboj/cdg022. PMC   140097 . PMID   12514124.
4. Lebars I, Husson C, Yoshizawa S, Douthwaite S, Fourmy D (September 2007). "Recognition elements in rRNA for the tylosin resistance methyltransferase RlmA(II)". Journal of Molecular Biology. 372 (2): 525–34. doi:10.1016/j.jmb.2007.06.068. PMID   17673230.
5. Douthwaite S, Jakobsen L, Yoshizawa S, Fourmy D (May 2008). "Interaction of the tylosin-resistance methyltransferase RlmA II at its rRNA target differs from the orthologue RlmA I". Journal of Molecular Biology. 378 (5): 969–75. doi:10.1016/j.jmb.2008.03.024. PMID   18406425.
6. Liu M, Douthwaite S (April 2002). "Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes Gram-negative from Gram-positive bacteria". Molecular Microbiology. 44 (1): 195–204. doi: 10.1046/j.1365-2958.2002.02866.x . PMID   11967079.

External links

• 23S+rRNA+(guanine748-N1)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)