| Rank | Hero | Sex | Actor(s) | Film | Year | Notes |
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| 1 | Atticus Finch | Male | Gregory Peck | To Kill a Mockingbird | 1962 | Received Academy Award |
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| 2 | Indiana Jones | Male | Harrison Ford (1) | Raiders of the Lost Ark | 1981 | |
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| 3 | James Bond | Male | Sean Connery | Dr. No | 1962 | |
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| 4 | Rick Blaine | Male | Humphrey Bogart (1) | Casablanca | 1942 | Nominated for Academy Award |
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| 5 | Marshal Will Kane | Male | Gary Cooper (1) | High Noon | 1952 | Received Academy Award |
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| 6 | Clarice Starling | Female | Jodie Foster | The Silence of the Lambs (1) | 1991 | Received Academy Award |
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| 7 | Rocky Balboa | Male | Sylvester Stallone | Rocky | 1976 | Nominated for Academy Award |
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| 8 | Ellen Ripley | Female | Sigourney Weaver | Aliens | 1986 | Nominated for Academy Award |
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| 9 | George Bailey | Male | James Stewart (1) | It's a Wonderful Life (1) | 1946 | Nominated for Academy Award |
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| 10 | T. E. Lawrence | Male | Peter O'Toole | Lawrence of Arabia | 1962 | Historical figure; nominated for Academy Award |
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| 11 | Jefferson Smith | Male | James Stewart (2) | Mr. Smith Goes to Washington | 1939 | Nominated for Academy Award |
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| 12 | Tom Joad | Male | Henry Fonda (1) | The Grapes of Wrath | 1940 | Nominated for Academy Award |
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| 13 | Oskar Schindler | Male | Liam Neeson | Schindler's List (1) | 1993 | Historical figure; nominated for Academy Award |
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| 14 | Han Solo | Male | Harrison Ford (2) | Star Wars (1) | 1977 | |
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| 15 | Norma Rae Webster | Female | Sally Field | Norma Rae | 1979 | Based upon southern mill worker Crystal Lee Sutton; received Academy Award |
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| 16 | Shane | Male | Alan Ladd | Shane | 1953 | |
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| 17 | Harry Callahan | Male | Clint Eastwood | Dirty Harry | 1971 | |
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| 18 | Robin Hood | Male | Errol Flynn | The Adventures of Robin Hood | 1938 | |
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| 19 | Virgil Tibbs | Male | Sidney Poitier | In the Heat of the Night | 1967 | |
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| 20 | Butch Cassidy and the Sundance Kid | Male | Paul Newman (1) and Robert Redford (1) | Butch Cassidy and the Sundance Kid | 1969 | Historical figures |
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| 21 | Mahatma Gandhi | Male | Ben Kingsley | Gandhi | 1982 | Historical figure; received Academy Award |
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| 22 | Spartacus | Male | Kirk Douglas | Spartacus | 1960 | Historical figure |
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| 23 | Terry Malloy | Male | Marlon Brando | On the Waterfront | 1954 | Received Academy Award |
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| 24 | Thelma Dickinson and Louise Sawyer | Female | Geena Davis and Susan Sarandon | Thelma & Louise | 1991 | Both nominated for Academy Award |
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| 25 | Lou Gehrig | Male | Gary Cooper (2) | The Pride of the Yankees | 1942 | Historical figure; nominated for Academy Award |
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| 26 | Superman | Male | Christopher Reeve | Superman | 1978 | |
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| 27 | Bob Woodward and Carl Bernstein | Male | Robert Redford (2) and Dustin Hoffman | All the President's Men | 1976 | Historical figures |
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| 28 | Juror #8 (Davis) | Male | Henry Fonda (2) | 12 Angry Men | 1957 | |
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| 29 | General George Patton | Male | George C. Scott | Patton | 1970 | Historical figure; received Academy Award |
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| 30 | Lucas "Luke" Jackson | Male | Paul Newman (2) | Cool Hand Luke | 1967 | Nominated for Academy Award |
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| 31 | Erin Brockovich | Female | Julia Roberts | Erin Brockovich | 2000 | Historical figure; received Academy Award |
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| 32 | Philip Marlowe | Male | Humphrey Bogart (2) | The Big Sleep | 1946 | |
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| 33 | Marge Gunderson | Female | Frances McDormand | Fargo | 1996 | Received Academy Award |
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| 34 | Tarzan | Male | Johnny Weissmuller | Tarzan the Ape Man | 1932 | |
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| 35 | Alvin York | Male | Gary Cooper (3) | Sergeant York | 1941 | Historical figure; received Academy Award |
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| 36 | Rooster Cogburn | Male | John Wayne | True Grit | 1969 | Received Academy Award |
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| 37 | Obi-Wan Kenobi | Male | Alec Guinness | Star Wars (2) | 1977 | Nominated for Academy Award |
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| 38 | The Tramp | Male | Charlie Chaplin | City Lights | 1931 | |
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| 39 | Lassie | Female | Pal | Lassie Come Home | 1943 | |
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| 40 | Frank Serpico | Male | Al Pacino (1) | Serpico | 1973 | Historical figure; nominated for Academy Award |
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| 41 | Arthur Chipping | Male | Robert Donat | Goodbye, Mr. Chips | 1939 | Received Academy Award |
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| 42 | Father Edward Flanagan | Male | Spencer Tracy | Boys Town | 1938 | Historical figure; received Academy Award |
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| 43 | Moses | Male | Charlton Heston | The Ten Commandments | 1956 | Biblical figure |
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| 44 | Jimmy "Popeye" Doyle | Male | Gene Hackman | The French Connection | 1971 | Based upon New York City Police Detective Eddie Egan; received Academy Award |
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| 45 | Zorro | Male | Tyrone Power | The Mark of Zorro | 1940 | |
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| 46 | Batman | Male | Michael Keaton | Batman (1) | 1989 | |
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| 47 | Karen Silkwood | Female | Meryl Streep | Silkwood | 1983 | Historical figure; nominated for Academy Award |
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| 48 | The Terminator | Male in appearance | Arnold Schwarzenegger (1) | Terminator 2: Judgment Day | 1991 | Character also appears on Villains list. [a] |
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| 49 | Andrew Beckett | Male | Tom Hanks | Philadelphia | 1993 | Loosely based upon Geoffrey Bowers; received Academy Award for performance |
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| 50 | General Maximus Decimus Meridius | Male | Russell Crowe | Gladiator | 2000 | Received Academy Award |
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