Acids in wine

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Malic and tartaric acid are the primary acids in wine grapes. Wine grape diagram en.svg
Malic and tartaric acid are the primary acids in wine grapes.

The acids in wine are an important component in both winemaking and the finished product of wine. They are present in both grapes and wine, having direct influences on the color, balance and taste of the wine as well as the growth and vitality of yeast during fermentation and protecting the wine from bacteria. The measure of the amount of acidity in wine is known as the “titratable acidity” or “total acidity”, which refers to the test that yields the total of all acids present, while strength of acidity is measured according to pH, with most wines having a pH between 2.9 and 3.9. Generally, the lower the pH, the higher the acidity in the wine. There is no direct connection between total acidity and pH (it is possible to find wines with a high pH for wine and high acidity). [1] In wine tasting, the term “acidity” refers to the fresh, tart and sour attributes of the wine which are evaluated in relation to how well the acidity balances out the sweetness and bitter components of the wine such as tannins. Three primary acids are found in wine grapes: tartaric, malic, and citric acids. During the course of winemaking and in the finished wines, acetic, butyric, lactic, and succinic acids can play significant roles. Most of the acids involved with wine are fixed acids with the notable exception of acetic acid, mostly found in vinegar, which is volatile and can contribute to the wine fault known as volatile acidity. Sometimes, additional acids, such as ascorbic, sorbic and sulfurous acids, are used in winemaking. [2]

Contents

Tartaric

While normally clear, tartaric crystals (pictured) can be dyed the color of the wine in which it has been saturated. HomemadeTartaric.jpg
While normally clear, tartaric crystals (pictured) can be dyed the color of the wine in which it has been saturated.

Tartaric acid is, from a winemaking perspective, the most important in wine due to the prominent role it plays in maintaining the chemical stability of the wine and its color and finally in influencing the taste of the finished wine. In most plants, this organic acid is rare, but it is found in significant concentrations in grape vines. Along with malic acid, and to a lesser extent citric acid, tartaric is one of the fixed acids found in wine grapes. The concentration varies depending on grape variety and the soil content of the vineyard. Some varieties, such as Palomino, are naturally disposed to having high levels of tartaric acids, [3] while Malbec and Pinot noir generally have lower levels. During flowering, high levels of tartaric acid are concentrated in the grape flowers and then young berries. As the vine progresses through ripening, tartaric does not get metabolized through respiration like malic acid, so the levels of tartaric acid in the grape vines remain relatively consistent throughout the ripening process. [4]

Less than half of the tartaric acid found in grapes is free standing, with the majority of the concentration present as potassium acid salt. During fermentation, these tartrates bind with the lees, pulp debris and precipitated tannins and pigments. While some variance among grape varieties and wine regions exists, generally about half of the deposits are soluble in the alcoholic mixture of wine. The crystallization of these tartrates can happen at unpredictable times, and in a wine bottle may appear like broken glass, though they are in fact harmless. Winemakers will often put the wine through cold stabilization, where it is exposed to temperatures below freezing to encourage the tartrates to crystallize and precipitate out of the wine, [4] or electrodialysis which removes the tartrates via a membrane process.

Malic

Riesling from cool climate wine regions, such as the Rheingau in Germany will have more malic acid and green apple notes than wines from warmer regions. Riesling Kabinett 1975 in glass.jpg
Riesling from cool climate wine regions, such as the Rheingau in Germany will have more malic acid and green apple notes than wines from warmer regions.

Malic acid, along with tartaric acid, is one of the principal organic acids found in wine grapes. It is found in nearly every fruit and berry plant, but is most often associated with green (unripe) apples, the flavor it most readily projects in wine. Its name comes from the Latin malum meaning “apple”. In the grape vine, malic acid is involved in several processes which are essential for the health and sustainability of the vine. Its chemical structure allows it to participate in enzymatic reactions that transport energy throughout the vine. Its concentration varies depending on the grape variety, with some varieties, such as Barbera, Carignan and Sylvaner, being naturally disposed to high levels. The levels of malic acid in grape berries are at their peak just before veraison, when they can be found in concentrations as high as 20  g/L. As the vine progresses through the ripening stage, malic acid is metabolized in the process of respiration, and by harvest, its concentration could be as low as 1 to 9 g/L. The respiratory loss of malic acid is more pronounced in warmer climates. When all the malic acid is used up in the grape, it is considered “over-ripe” or senescent. Winemakers must compensate for this loss by adding extraneous acid at the winery in a process known as acidification. [5]

Malic acid can be further reduced during the winemaking process through malolactic fermentation or MLF. In this process, bacteria convert the stronger malic acid into the softer lactic acid; formally, malic acid is polyprotic (contributes multiple protons, here two), while lactic acid is monoprotic (contributes one proton), and thus has only half the effect on acidity (pH); also, the first acidity constant (pKa) of malic acid (3.4 at room temperature) is lower than the (single) acidity constant of lactic acid (3.86 at room temperature), indicating stronger acidity. Thus after MLF, wine has a higher pH (less acidic), and a different mouthfeel.

The bacteria behind this process can be found naturally in the winery, in cooperages, which make oak wine barrels that will house a population of the bacteria or they can be introduced by the winemaker with a cultured specimen. For some wines, the conversion of malic into lactic acid can be beneficial, especially if the wine has excessive levels of malic acid. For other wines, such as Chenin blanc and Riesling, it produces off flavors in the wine (such as the buttery smell of diacetyl) that would not be appealing for that variety. In general, red wines are more often put through MLF than whites, which means a higher likelihood of finding malic acid in white wines (though notable exceptions, such as oaked Chardonnay, are often put through MLF). [5]

Lactic

Chardonnay is often put through malolactic fermentation when it is being oaked, such as via oak chips as pictured. The softer, milky lactic acid helps contribute to a creamier mouthfeel in the wine. Oak chips in chardonnay.jpg
Chardonnay is often put through malolactic fermentation when it is being oaked, such as via oak chips as pictured. The softer, milky lactic acid helps contribute to a creamier mouthfeel in the wine.

A much milder acid than tartaric and malic, lactic acid is often associated with “milky” flavors in wine and is the primary acid of yogurt and sauerkraut. It is produced during winemaking by lactic acid bacteria (LAB), which includes three genera: Oenococcus, Pediococcus and Lactobacillus . These bacteria convert both sugar and malic acid into lactic acid, the latter through MLF. This process can be beneficial for some wines, adding complexity and softening the harshness of malic acidity, but it can generate off flavors and turbidity in others. Some strains of LAB can produce biogenic amines, such as histamine, tyramine and putrescine, which may be a cause of red wine headaches in some wine drinkers. Winemakers wishing to control or prevent MLF can use sulfur dioxide to stun the bacteria. Racking the wine quickly off its lees will also help control the bacteria, since lees are a vital food source for them. The winemakers must also be very careful of what wine barrels and winemaking equipment to which the wine is exposed, because of the bacteria's ability to deeply embed themselves within wood fibers. A wine barrel that has completed one successful malolactic fermentation will almost always induce MLF in every wine stored in it from then on. [6]

Citric

While very common in citrus fruits, such as limes, citric acid is found only in very minute quantities in wine grapes. It often has a concentration about 1/20 that of tartaric acid. The citric acid most commonly found in wine is commercially produced acid supplements derived from fermenting sucrose solutions. These inexpensive supplements can be used by winemakers in acidification to boost the wine's total acidity. It is used less frequently than tartaric and malic due to the aggressive citric flavors it can add to the wine. When citric acid is added, it is always done after primary alcohol fermentation has been completed due to the tendency of yeast to convert citric into acetic acid. In the European Union, use of citric acid for acidification is prohibited, but limited use of citric acid is permitted for removing excess iron and copper from the wine if potassium ferrocyanide is not available. [7]

Acetic

Acetic acid is a two-carbon organic acid produced in wine during or after the fermentation period. It is the most volatile of the primary acids associated with wine and is responsible for the sour taste of vinegar. During fermentation, activity by yeast cells naturally produces a small amount of acetic acid. If the wine is exposed to oxygen, Acetobacter bacteria will convert the ethanol into acetic acid. This process is known as the “acetification” of wine and is the primary process behind wine degradation into vinegar. An excessive amount of acetic acid is also considered a wine fault. A taster's sensitivity to acetic acid will vary, but most people can detect excessive amounts at around 600  mg/L. [2]

Ascorbic

Ascorbic acid, also known as vitamin C, is found in young wine grapes prior to veraison, but is rapidly lost throughout the ripening process. In winemaking, it is used with sulfur dioxide as an antioxidant, often added during the bottling process for white wines. In the European Union, use of ascorbic acid as an additive is limited to 150 mg/L. [8]

The smell of crushed Pelargonium geranium leaves is a sign that a wine has a wine fault derived from sorbic acid. Geranium E19.jpg
The smell of crushed Pelargonium geranium leaves is a sign that a wine has a wine fault derived from sorbic acid.

Butyric

Butyric acid is a bacteria-induced wine fault that can cause a wine to smell of spoiled Camembert or rancid butter. [9]

Sorbic

Sorbic acid is a winemaking additive used often in sweet wines as a preservative against fungi, bacteria and yeast growth. Unlike sulfur dioxide, it does not hinder the growth of the lactic acid bacteria. In the European Union, the amount of sorbic acid that can be added is limited — no more than 200 mg/L. Most humans have a detection threshold of 135 mg/L, with some having a sensitivity to detect its presence at 50 mg/L. Sorbic acid can produce off flavors and aromas which can be described as “rancid”. When lactic acid bacteria metabolize sorbates in the wine, it creates a wine fault that is most recognizable by an aroma of crushed Pelargonium geranium leaves. [10]

Succinic

Succinic acid is most commonly found in wine, but can also be present in trace amounts in ripened grapes. While concentration varies among grape varieties, it is usually found in higher levels with red wine grapes. The acid is created as a byproduct of the metabolization of nitrogen by yeast cells during fermentation. The combination of succinic acid with one molecule of ethanol will create the ester ethyl succinate which contributes to a mild fruity aroma in wines. [11]

Effects

Making

A wine with high pH and low acidity like Carmenere (pictured) will have more bluish color notes than a wine with high acidity. Crop carmenere close up.JPG
A wine with high pH and low acidity like Carménère (pictured) will have more bluish color notes than a wine with high acidity.

Acidity is highest in wine grapes just before the start of veraison , which ushers in the ripening period of the annual cycle of grape vines. As the grapes ripen, their sugar levels increase and their acidity levels decrease. Through the process of respiration, malic acid is metabolized by the grape vine. Grapes from cooler climate wine regions generally have higher levels of acidity due to the slower ripening process. The level of acidity still present in the grape is an important consideration for winemakers in deciding when to begin harvest. For wines such as Champagne and other sparkling wines, having high levels of acidity is even more vital to the winemaking process, so grapes are often picked under-ripe and at higher acid levels. [2]

In the winemaking process, acids aid in enhancing the effectiveness of sulfur dioxide to protect the wines from spoilage and can also protect the wine from bacteria due to the inability of most bacteria to survive in low pH solutions. Two notable exceptions to this are Acetobacter and the lactic acid bacteria. In red wines, acidity helps preserve and stabilize the color of the wine. The ionization of anthocyanins is affected by pH, so wines with lower pH (such as Sangiovese-based wines) have redder, more stable colors. Wines with higher pH (such as Syrah-based wines) have higher levels of less stable blue pigments, eventually taking on a muddy grey hue. These wines can also develop a brownish tinge. In white wines, higher pH (lower acidity) causes the phenolics in the wine to darken and eventually polymerize as brown deposits. [2]

Winemakers will sometimes add acids to the wine (acidification) to make the wine more acidic, most commonly in warm climate regions where grapes are often harvested at advanced stages of ripeness with high levels of sugars, but very low levels of acid. Tartaric acid is most often added, but winemakers will sometimes add citric or malic acid. Acids can be added either before or after primary fermentation. They can be added during blending or aging, but the increased acidity will become more noticeable to wine tasters if added at this point. [2]

Tasting

The acidity in wine is an important component in the quality and taste of the wine. It adds a sharpness to the flavors and is detected most readily by a prickling sensation on the sides of the tongue and a mouth-watering aftertaste. Of particular importance is the balance of acidity versus the sweetness of the wine (the leftover residual sugar) and the more bitter components of the wine (most notably tannins but also includes other phenolics). A wine with too much acidity will taste excessively sour and sharp. A wine with too little acidity will taste flabby and flat, with less defined flavors. [2]

See also

Related Research Articles

<span class="mw-page-title-main">Chardonnay</span> Variety of grape mainly used to make wine

Chardonnay is a green-skinned grape variety used in the production of white wine. The variety originated in the Burgundy wine region of eastern France, but is now grown wherever wine is produced, from England to New Zealand. For new and developing wine regions, growing Chardonnay is seen as a 'rite of passage' and an easy entry into the international wine market.

<span class="mw-page-title-main">Winemaking</span> Production of wine

Winemaking or vinification is the production of wine, starting with the selection of the fruit, its fermentation into alcohol, and the bottling of the finished liquid. The history of wine-making stretches over millennia. The science of wine and winemaking is known as oenology. A winemaker may also be called a vintner. The growing of grapes is viticulture and there are many varieties of grapes.

<span class="mw-page-title-main">Carbonic maceration</span> Winemaking technique

Carbonic maceration is a winemaking technique, often associated with the French wine region of Beaujolais, in which whole grapes are fermented in a carbon dioxide rich environment before crushing. Conventional alcoholic fermentation involves crushing the grapes to free the juice and pulp from the skin with yeast serving to convert sugar into ethanol. Carbonic maceration ferments most of the juice while it is still inside the grape, although grapes at the bottom of the vessel are crushed by gravity and undergo conventional fermentation. The resulting wine is fruity with very low tannins. It is ready to drink quickly but lacks the structure for long-term aging. In extreme cases such as Beaujolais nouveau, the period between picking and bottling can be less than six weeks.

<span class="mw-page-title-main">Red wine</span> Wine made from dark-colored grape varieties

Red wine is a type of wine made from dark-colored grape varieties. The color of the wine can range from intense violet, typical of young wines, through to brick red for mature wines and brown for older red wines. The juice from most purple grapes is greenish-white, the red color coming from anthocyan pigments present in the skin of the grape. Much of the red wine production process involves extraction of color and flavor components from the grape skin.

<span class="mw-page-title-main">Malolactic fermentation</span> Process in winemaking

Malolactic conversion is a process in winemaking in which tart-tasting malic acid, naturally present in grape must, is converted to softer-tasting lactic acid. Malolactic fermentation is most often performed as a secondary fermentation shortly after the end of the primary fermentation, but can sometimes run concurrently with it. The process is standard for most red wine production and common for some white grape varieties such as Chardonnay, where it can impart a "buttery" flavor from diacetyl, a byproduct of the reaction.

<span class="mw-page-title-main">Agiorgitiko</span> Variety of grape

Agiorgitiko is a red Greek wine grape variety that, as of 2012, was the most widely planted red grape variety in Greece, ahead of Xynomavro. The grape has traditionally been grown in the Nemea region of the Peloponnese but can be found throughout the country including Attikí (Attica) and Makedonía (Macedonia).

<span class="mw-page-title-main">Maceration (wine)</span> Winemaking process where grape skins and seeds are kept in contact with the juice

Maceration is the winemaking process where the phenolic materials of the grape—tannins, coloring agents (anthocyanins) and flavor compounds—are leached from the grape skins, seeds and stems into the must. To macerate is to soften by soaking, and maceration is the process by which the red wine receives its red color, since raw grape juice is clear-grayish in color. In the production of white wines, maceration is either avoided or allowed only in very limited manner in the form of a short amount of skin contact with the juice prior to pressing. This is more common in the production of varietals with less natural flavor and body structure like Sauvignon blanc and Sémillon. For Rosé, red wine grapes are allowed some maceration between the skins and must, but not to the extent of red wine production.

A wine fault or defect is an unpleasant characteristic of a wine often resulting from poor winemaking practices or storage conditions, and leading to wine spoilage. Many of the compounds that cause wine faults are already naturally present in wine but at insufficient concentrations to be of issue. In fact, depending on perception, these concentrations may impart positive characters to the wine. However, when the concentration of these compounds greatly exceeds the sensory threshold, they replace or obscure the flavors and aromas that the wine should be expressing. Ultimately the quality of the wine is reduced, making it less appealing and sometimes undrinkable.

Secondary fermentation is a process commonly associated with winemaking, which entails a second period of fermentation in a different vessel than the one used to start the fermentation process. An example of this would be starting fermentation in a carboy or stainless steel tank and then moving it over to oak barrels. Rather than being a separate, second fermentation, this is most often one single fermentation period that is conducted in multiple vessels. However, the term does also apply to procedures that could be described as a second and distinct fermentation period.

<span class="mw-page-title-main">Fermentation in winemaking</span> Wine making process

The process of fermentation in winemaking turns grape juice into an alcoholic beverage. During fermentation, yeasts transform sugars present in the juice into ethanol and carbon dioxide. In winemaking, the temperature and speed of fermentation are important considerations as well as the levels of oxygen present in the must at the start of the fermentation. The risk of stuck fermentation and the development of several wine faults can also occur during this stage, which can last anywhere from 5 to 14 days for primary fermentation and potentially another 5 to 10 days for a secondary fermentation. Fermentation may be done in stainless steel tanks, which is common with many white wines like Riesling, in an open wooden vat, inside a wine barrel and inside the wine bottle itself as in the production of many sparkling wines.

<span class="mw-page-title-main">Wine tasting descriptors</span>

The use of wine tasting descriptors allows the taster to qualitatively relate the aromas and flavors that the taster experiences and can be used in assessing the overall quality of wine. Wine writers differentiate wine tasters from casual enthusiasts; tasters attempt to give an objective description of the wine's taste, casual enthusiasts appreciate wine but pause their examination sooner than tasters. The primary source of a person's ability to taste wine is derived from their olfactory senses. A taster's own personal experiences play a significant role in conceptualizing what they are tasting and attaching a description to that perception. The individual nature of tasting means that descriptors may be perceived differently among various tasters.

<span class="mw-page-title-main">Harvest (wine)</span> Harvest of grapes in order to produce wine

The harvesting of wine grapes (Vintage) is one of the most crucial steps in the process of wine-making. The time of harvest is determined primarily by the ripeness of the grape as measured by sugar, acid and tannin levels with winemakers basing their decision to pick based on the style of wine they wish to produce. The weather can also shape the timetable of harvesting with the threat of heat, rain, hail, and frost which can damage the grapes and bring about various vine diseases. In addition to determining the time of the harvest, winemakers and vineyard owners must also determine whether to use hand pickers or mechanical harvesters. The harvest season typically falls between August & October in the Northern Hemisphere and February & April in the Southern Hemisphere. With various climate conditions, grape varieties, and wine styles the harvesting of grapes could happen in every month of the calendar year somewhere in the world. In the New World it is often referred to as the crush.

<span class="mw-page-title-main">Annual growth cycle of grapevines</span> Process that takes place in the vineyard each year

The annual growth cycle of grapevines is the process that takes place in the vineyard each year, beginning with bud break in the spring and culminating in leaf fall in autumn followed by winter dormancy. From a winemaking perspective, each step in the process plays a vital role in the development of grapes with ideal characteristics for making wine. Viticulturalists and vineyard managers monitor the effect of climate, vine disease and pests in facilitating or impeding the vine's progression from bud break, flowering, fruit set, veraison, harvesting, leaf fall and dormancy-reacting if need be with the use of viticultural practices like canopy management, irrigation, vine training and the use of agrochemicals. The stages of the annual growth cycle usually become observable within the first year of a vine's life. The amount of time spent at each stage of the growth cycle depends on a number of factors-most notably the type of climate and the characteristics of the grape variety.

<span class="mw-page-title-main">Sugars in wine</span>

Sugars in wine are at the heart of what makes winemaking possible. During the process of fermentation, sugars from wine grapes are broken down and converted by yeast into alcohol (ethanol) and carbon dioxide. Grapes accumulate sugars as they grow on the grapevine through the translocation of sucrose molecules that are produced by photosynthesis from the leaves. During ripening the sucrose molecules are hydrolyzed (separated) by the enzyme invertase into glucose and fructose. By the time of harvest, between 15 and 25% of the grape will be composed of simple sugars. Both glucose and fructose are six-carbon sugars but three-, four-, five- and seven-carbon sugars are also present in the grape. Not all sugars are fermentable, with sugars like the five-carbon arabinose, rhamnose and xylose still being present in the wine after fermentation. Very high sugar content will effectively kill the yeast once a certain (high) alcohol content is reached. For these reasons, no wine is ever fermented completely "dry". Sugar's role in dictating the final alcohol content of the wine sometimes encourages winemakers to add sugar during winemaking in a process known as chaptalization solely in order to boost the alcohol content – chaptalization does not increase the sweetness of a wine.

This glossary of winemaking terms lists some of terms and definitions involved in making wine, fruit wine, and mead.

<span class="mw-page-title-main">Clarification and stabilization of wine</span> Wine clarification and stabilisation

In winemaking, clarification and stabilization are the processes by which insoluble matter suspended in the wine is removed before bottling. This matter may include dead yeast cells (lees), bacteria, tartrates, proteins, pectins, various tannins and other phenolic compounds, as well as pieces of grape skin, pulp, stems and gums. Clarification and stabilization may involve fining, filtration, centrifugation, flotation, refrigeration, pasteurization, and/or barrel maturation and racking.

<span class="mw-page-title-main">Ripeness in viticulture</span> How the term "ripe" is used in viticulture and winemaking

In viticulture, ripeness is the completion of the ripening process of wine grapes on the vine which signals the beginning of harvest. What exactly constitutes ripeness will vary depending on what style of wine is being produced and what the winemaker and viticulturist personally believe constitutes ripeness. Once the grapes are harvested, the physical and chemical components of the grape which will influence a wine's quality are essentially set so determining the optimal moment of ripeness for harvest may be considered the most crucial decision in winemaking.

<span class="mw-page-title-main">Yeast in winemaking</span> Yeasts used for alcoholic fermentation of wine

The role of yeast in winemaking is the most important element that distinguishes wine from fruit juice. In the absence of oxygen, yeast converts the sugars of the fruit into alcohol and carbon dioxide through the process of fermentation. The more sugars in the grapes, the higher the potential alcohol level of the wine if the yeast are allowed to carry out fermentation to dryness. Sometimes winemakers will stop fermentation early in order to leave some residual sugars and sweetness in the wine such as with dessert wines. This can be achieved by dropping fermentation temperatures to the point where the yeast are inactive, sterile filtering the wine to remove the yeast or fortification with brandy or neutral spirits to kill off the yeast cells. If fermentation is unintentionally stopped, such as when the yeasts become exhausted of available nutrients and the wine has not yet reached dryness, this is considered a stuck fermentation.

<span class="mw-page-title-main">Yeast assimilable nitrogen</span> Form of nitrogen available to wine yeast to use during fermentation

Yeast assimilable nitrogen or YAN is the combination of free amino nitrogen (FAN), ammonia (NH3) and ammonium (NH4+) that is available for a yeast, e.g. the wine yeast Saccharomyces cerevisiae, to use during fermentation. Outside of the fermentable sugars glucose and fructose, nitrogen is the most important nutrient needed to carry out a successful fermentation that doesn't end prior to the intended point of dryness or sees the development of off-odors and related wine faults. To this extent winemakers will often supplement the available YAN resources with nitrogen additives such as diammonium phosphate (DAP).

<span class="mw-page-title-main">Wine preservatives</span> Food preservation

Wine preservatives are used to preserve the quality and shelf life of bottled wine without affecting its taste. Specifically, they are used to prevent oxidation and bacterial spoilage by inhibiting microbial activity.

References

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