David Klenerman

Last updated

Professor
Sir David Klenerman
FRS FMedSci
David Klenerman Studio shoot 30 05 24 photos taken by Michael Webb (c)University of Cambridge R6A 2299.jpg
BornSeptember 1959 (age 65) [1]
NationalityBritish
Alma mater University of Cambridge (MA, PhD)
Known for Illumina dye sequencing
Scanning ion-conductance microscopy
Super-resolution microscopy
Solexa
Children2 (Laura & Anna)
Awards
Scientific career
Fields Biophysical chemistry
Institutions University of Cambridge
Stanford University
Thesis Infrared chemiluminescence using a SISAM spectrometer  (1985)
Doctoral advisor Ian William Murison Smith
Other academic advisors Richard Zare
Website klenermangroup.co.uk

Sir David Klenerman (born 1959) is a British biophysical chemist and a professor of biophysical chemistry at the Department of Chemistry at the University of Cambridge [3] and a Fellow of Christ's College, Cambridge. [4]

Contents

He is best known for his contribution in the field of next-generation sequencing of DNA (that subsequently resulted in Solexa, a high-speed DNA sequencing company that he co-founded), [1] [5] [6] [7] [8] nanopipette-based scanning ion-conductance microscopy, [9] [10] and super-resolution microscopy. [11]

Early life and education

Klenerman is the son of two South African-born Jews. [12] He was educated at the University of Cambridge where he was an undergraduate student of Christ's College, Cambridge and received his BA degree in 1982. [13] He earned his PhD degree in chemistry in 1986 as a postgraduate student of Churchill College, Cambridge and was supervised by Ian William Murison Smith. [14] [2] [13]

Career and research

After his doctorate, Klenerman went to Stanford University as a Fulbright scholar to work on high-overtone chemistry, with Richard Zare. After his postdoctoral research at Stanford, he returned to United Kingdom to work in BP Research for seven years. Then, in 1994, he joined the University of Cambridge, as a faculty member of the Department of Chemistry and a fellow of Christ's College. [2] [13] [15]

Klenerman, along with Shankar Balasubramanian, invented a method of next-generation DNA sequencing which is commonly known today as the Solexa sequencing or Illumina dye sequencing. [5] [7] The method is based on the detection of fluorophore labelled nucleotides as they get incorporated in the DNA strands. [16] This sequencing by synthesis method gained popularity, [17] [18] and is currently regarded as the most widely used platform to replace conventional Sanger sequencing technique, despite its comparatively low multiplexing capability of samples, as it offers several key advantages: it is automated, quick, highly accurate, capable of sequencing multiple strands simultaneously via massive parallel sequencing, and economically cheaper in case of whole genome sequencing. [19] [20] [21]

He is also known for exploring nanopipette-based (instead of conventional micropipette-based) scanning ion-conductance microscopy methods. [9] [22] His research group was successful in achieving very high resolution topographic images of live-cells, in hopping mode imaging, in precise delivery of small molecules to cell, and in studying real time detailed cell-functioning. [10] [23] [24]

Most recently, his group is focusing on 3D super-resolution microscopy to develop new insights on protein misfolding and neurodegenerative diseases. [11]

Commercial activities

Klenerman and Shankar Balasubramanian commercialised their invention on the single-molecule-fluorescence based high-speed DNA sequencing and jointly founded Solexa in 1998. Later, in 2007, this company was acquired by Illumina for $600 million. [5] [25] [26] [27] [28]

In 2004, Klenerman co-founded another spin-out company, Ionscope, to supply assembled scanning ion-conductance microscopes to the research community that looks for high-resolution 3D images of live cells. As per the Biotechnology and Biological Sciences Research Council, as of February 2014, Ionscope sold 35 SICM units worldwide. [29]

Awards and honours

The major awards and honours that Klenerman received in recognition of his research work:

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References

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  2. 1 2 3 4 "Interdisciplinary Award 2007 Winner". Royal Society of Chemistry . Retrieved 26 January 2016.
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  4. ↑ "Professor David Klenerman". Christ's College, Cambridge . Retrieved 26 January 2016.
  5. 1 2 3 "History of Illumina Sequencing". Illumina . Retrieved 26 January 2016.
  6. ↑ Davies, Kevin (7 September 2010). The $1,000 Genome . Free Press. pp.  102–115. ISBN   978-1416569596 . Retrieved 26 January 2016.
  7. 1 2 Bentley, DR; Balasubramanian, S; Swerdlow, HP; et al. (6 November 2008). "Accurate whole human genome sequencing using reversible terminator chemistry". Nature . 456 (7218): 53–59. Bibcode:2008Natur.456...53B. doi:10.1038/nature07517. PMC   2581791 . PMID   18987734.
  8. ↑ "The Solexa Story". Bio-IT World. No. September–October 2010. 28 September 2010. Archived from the original on 20 February 2016. Retrieved 26 January 2016.
  9. 1 2 "Nanodoodling shows pipette power". BBC News . 5 September 2006. Retrieved 26 January 2016.
  10. 1 2 "Drawing With DNA: Nanopipette allows voltage-controlled delivery of biomolecules to a surface". Chemical & Engineering News . Vol. 83, no. 44. American Chemical Society. 31 October 2005. Retrieved 26 January 2016.
  11. 1 2 Hell, Stefan W.; Sahl, Steffen J.; Bates, Mark; Zhuang, Xiaowei; Heintzmann, Rainer; Booth, Martin J.; Bewersdorf, Joerg; Shtengel, Gleb; Hess, Harald; Tinnefeld, Philip; Honigmann, Alf; Jakobs, Stefan; Testa, Ilaria; Cognet, Laurent; Lounis, Brahim; Ewers, Helge; Davis, Simon J.; Eggeling, Christian; Klenerman, David; Willig, Katrin I.; Vicidomini, Giuseppe; Castello, Marco; Diaspro, Alberto; Cordes, Thorben; Steffen J. Sahl; Tinnefeld, Philip; Klenerman, David; Katrin I. Willig (14 October 2015). "The 2015 super-resolution microscopy roadmap". Journal of Physics D . 48 (44): 443001. arXiv: 1711.04999 . Bibcode:2015JPhD...48R3001H. doi:10.1088/0022-3727/48/44/443001. S2CID   4804015.
  12. ↑ Frazer, Jenni (28 December 2018). "Seven Holocaust survivors on New Year's Honours List". Jewish News . Retrieved 14 February 2024.
  13. 1 2 3 Anon (2017). "Klenerman, Prof. David" . Who's Who (online Oxford University Press  ed.). Oxford: A & C Black. doi:10.1093/ww/9780199540884.013.266724.(Subscription or UK public library membership required.)
  14. ↑ Klenerman, David (1985). Infrared chemiluminescence using a SISAM spectrometer. lib.cam.ac.uk (PhD thesis). University of Cambridge. OCLC   499899771. EThOS   uk.bl.ethos.355881.
  15. ↑ "David Klenerman elected FRS". Christ's College, Cambridge. Archived from the original on 25 August 2017. Retrieved 27 January 2016.
  16. ↑ Balasubramanian, Shankar (4 May 2011). "Sequencing nucleic acids: from chemistry to medicine". Chemical Communications . 47 (26): 7281–7286. doi:10.1039/c1cc11078k. PMC   3428630 . PMID   21544287.
  17. ↑ Quail, Michael A (25 November 2008). "A large genome center's improvements to the Illumina sequencing system". Nature Methods . 5 (12): 1005–1010. doi:10.1038/nmeth.1270. PMC   2610436 . PMID   19034268.
  18. ↑ Cronn, Richard (27 August 2008). "Multiplex sequencing of plant chloroplast genomes using Solexa sequencing-by-synthesis technology". Nucleic Acids Research . 36 (19): e122. doi:10.1093/nar/gkn502. PMC   2577356 . PMID   18753151.
  19. ↑ Metzker, Michael L. (8 December 2009). "Sequencing technologies – the next generation". Nature Reviews Genetics . 11 (1): 31–46. CiteSeerX   10.1.1.719.3885 . doi:10.1038/nrg2626. PMID   19997069. S2CID   205484500.
  20. ↑ Pettersson, Erik; Lundeberg, Joakim; Ahmadian, Afshin (2009). "Generations of sequencing technologies". Genomics . 93 (2): 105–111. doi:10.1016/j.ygeno.2008.10.003. PMID   18992322.
  21. ↑ Quail, Michael A (24 July 2012). "A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers". BMC Genomics . 13 (341): 341. doi: 10.1186/1471-2164-13-341 . PMC   3431227 . PMID   22827831.
  22. ↑ Shevchuk, Andrew I (2011). "Realizing the biological and biomedical potential of nanoscale imaging using a pipette probe". Nanomedicine . 6 (3): 565–575. doi:10.2217/nnm.10.154. PMID   21542692.
  23. ↑ Chen, Chiao-Chen; Zhou, Yi; Baker, Lane A. (2012). "Scanning Ion Conductance Microscopy". Annual Review of Analytical Chemistry . 5 (207): 207–228. Bibcode:2012ARAC....5..207C. doi:10.1146/annurev-anchem-062011-143203. PMID   22524219.
  24. ↑ Bergner, Stefan; Vatsyayan, Preety; Matysik, Frank-Michael (2 May 2013). "Recent advances in high resolution scanning electrochemical microscopy of living cells – A review". Analytica Chimica Acta . 775: 1–13. Bibcode:2013AcAC..775....1B. doi:10.1016/j.aca.2012.12.042. PMID   23601970.
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  26. ↑ "$600m for DNA firm". Varsity . Retrieved 27 January 2016.
  27. ↑ "Illumina Buys Solexa". Forbes . 13 November 2006. Retrieved 27 January 2016.
  28. ↑ "Illumina to Buy Solexa For $600 Million in Stock". The Wall Street Journal . 13 November 2006. Retrieved 27 January 2016.
  29. ↑ "New microscope technology enables imaging of live cells". BBSRC . Retrieved 30 January 2016.
  30. ↑ "British Biophysical Society Lecture Tour". University College Dublin . Retrieved 27 January 2016.
  31. ↑ "David Klenerman: Royal Society". Fellows Directory. Royal Society . Retrieved 26 January 2016.
  32. ↑ "Fellow: Academy of Medical Sciences". Academy of Medical Sciences, United Kingdom. Archived from the original on 26 August 2017. Retrieved 26 January 2016.
  33. ↑ Royal Medal 2018, Royal society.org. Accessed 30 January 2023.
  34. ↑ "2019 New Year Honours List" (PDF). gov.uk. 31 December 2018.
  35. ↑ "Breakthrough Prize – Winners Of The 2022 Breakthrough Prizes In Life Sciences, Fundamental Physics And Mathematics Announced". breakthroughprize.org. Retrieved 13 October 2021.
  36. ↑ Canada Gairdner International Award 2024
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