Hypersensitive response

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Hypersensitive response (HR) is a mechanism used by plants to prevent the spread of infection by microbial pathogens. HR is characterized by the rapid death of cells in the local region surrounding an infection and it serves to restrict the growth and spread of pathogens to other parts of the plant. It is analogous to the innate immune system found in animals, and commonly precedes a slower systemic (whole plant) response, which ultimately leads to systemic acquired resistance (SAR). [1] HR can be observed in the vast majority of plant species and is induced by a wide range of plant pathogens such as oomycetes, viruses, fungi and even insects. [2]

Contents

Lesions caused by the plant hypersensitive response Plant hypersensitive response lesions.jpg
Lesions caused by the plant hypersensitive response

HR is commonly thought of as an effective defence strategy against biotrophic plant pathogens, which require living tissue to gain nutrients. In the case of necrotrophic pathogens, HR might even be beneficial to the pathogen, as they require dead plant cells to obtain nutrients. The situation becomes complicated when considering pathogens such as Phytophthora infestans which at the initial stages of the infection act as biotrophs but later switch to a necrotrophic lifestyle. It is proposed that in this case HR might be beneficial in the early stages of the infection but not in the later stages. [3]

Genetics

The first idea of how the hypersensitive response occurs came from Harold Henry Flor's gene-for-gene model. He postulated that for every resistance (R) gene encoded by the plant, there is a corresponding avirulence (Avr) gene encoded by the microbe. The plant is resistant to the pathogen if both the Avr and R genes are present during the plant-pathogen interaction. [4] The genes that are involved in the plant-pathogen interactions tend to evolve at a very rapid rate. [5]

Mechanism of plant NLR protein activation after pathogen invasion Plant NLR protein activation mechanism.png
Mechanism of plant NLR protein activation after pathogen invasion

Very often, the resistance mediated by R genes is due to them inducing HR, which leads to apoptosis. Most plant R genes encode NOD-like receptor (NLR) proteins. [6] NLR protein domain architecture consists of an NB-ARC domain which is a nucleotide-binding domain, responsible for conformational changes associated with the activation of the NLR protein. In the inactive form, the NB-ARC domain is bound to Adenosine diphosphate (ADP). When a pathogen is sensed, the ADP is exchanged for Adenosine triphosphate (ATP) and this induces a conformational change in the NLR protein, which results in HR. At the N-terminus, the NLR either has a Toll-Interleukin receptor (TIR) domain (also found in mammalian toll-like receptors) or a coiled-coil (CC) motif. Both TIR and CC domains are implicated in causing cell death during HR. The C-terminus of the NLRs consists of a leucine-rich repeat (LRR) motif, which is involved in sensing the pathogen virulence factors. [7]

Mechanism

HR is triggered by the plant when it recognizes a pathogen. The identification of a pathogen typically occurs when a virulence gene product, secreted by a pathogen, binds to, or indirectly interacts with the product of a plant R gene. R genes are highly polymorphic, and many plants produce several different types of R gene products, enabling them to recognize virulence products produced by many different pathogens. [8]

In phase one of the HR, the activation of R genes triggers an ion flux, involving an efflux of hydroxide and potassium to the outside the cells, and an influx of calcium and hydrogen ions into the cells. [9]

In phase two, the cells involved in the HR generate an oxidative burst by producing reactive oxygen species (ROS), superoxide anions, hydrogen peroxide, hydroxyl radicals and nitrous oxide. These compounds affect cellular membrane function, in part by inducing lipid peroxidation and by causing lipid damage. [9]

The alteration of ion components in the cell and the breakdown of cellular components in the presence of ROS result in the death of affected cells, as well as the formation of local lesions. Reactive oxygen species also trigger the deposition of lignin and callose, as well as the cross-linking of pre-formed hydroxyproline-rich glycoproteins such as P33 to the wall matrix via the tyrosine in the PPPPY motif. [9] These compounds serve to reinforce the walls of cells surrounding the infection, creating a barrier and inhibiting the spread of the infection. [10] Activation of HR also results in disruption of the cytoskeleton, mitochondrial function and metabolic changes, all of which might be implicated in causing cell death. [11] [12] [13]

Direct and indirect activation

HR can be activated in two main ways: directly and indirectly. Direct binding of the virulence factors to the NLRs can result in the activation of HR. However, this seems to be quite rare. More commonly, the virulence factors target certain cellular proteins that they modify and this modification is then sensed by NLRs. Indirect recognition seems to be more common as multiple virulence factors can modify the same cellular protein with the same modifications thus allowing one receptor to recognize multiple virulence factors. [14] Sometimes, the protein domains targeted by the virulence factors are integrated into the NLRs. An example of this can be observed in plant resistance to the rice blast pathogen, where the RGA5 NLR has a heavy-metal-associated (HMA) domain integrated into its structure, which is targeted by multiple effector proteins. [15]

An example of indirect recognition: AvrPphB is a type III effector protein secreted by Pseudomonas syringae . This is a protease which cleaves a cellular kinase called PBS1. The modified kinase is sensed by RPS5 NLR. [16]

The Resistosome

Recent structural studies of CC-NLR proteins have suggested that after the virulence factors are sensed, the NLRs assemble into a pentameric structure known as the resistosome. The resistosome seems to have a high affinity for the cellular membrane. When the resistosome is assembled, a helix sticks out from the N-terminus of each NLR and this creates a pore in the membrane which allows leakage of ions to occur and thus the cell dies. However, this mechanism is only inferred from the structure and there are currently no mechanistic studies to support this. It is still not known how the TIR-NLR proteins are activated. Recent research suggests that they require CC-NLR proteins downstream of them, which are then activated to form the resistosomes and induce HR. [17]

NLR pairs and networks

It is known that NLRs can function individually but there are also cases where the NLR proteins work in pairs. The pair consists of a sensor NLR and a helper NLR. The sensor NLR is responsible for recognizing the pathogen secreted effector protein and activating the helper NLR which then executes the cell death. The genes of both the sensor and the respective helper NLR are usually paired in the genome and their expression could be controlled by the same promoter. This allows the functional pair, instead of individual components, to be segregated during cell division and also ensures that equal amounts of both NLRs are made in the cell. [18]

The receptor pairs work through two main mechanisms: negative regulation or cooperation.

In the negative regulation scenario, the sensor NLR is responsible for negatively regulating the helper NLR and preventing cell death under normal conditions. However, when the effector protein is introduced and recognized by the sensor NLR, the negative regulation of the helper NLR is relieved and HR is induced. [19]

In the cooperation mechanisms, when the sensor NLR recognizes the effector protein it signals to the helper NLR, thus activating it. [20]

Recently, it was discovered that in addition to acting as singletons or pairs, the plant NLRs can act in networks. In these networks, there are usually many sensor NLRs paired to relatively few helper NLRs. [20]

NLR Singleton, Pair and Network NLR Singleton, Pair and Network.png
NLR Singleton, Pair and Network

One example of proteins involved in NLR networks are those belonging to the NRC superclade. It seems that the networks evolved from a duplication event of a genetically linked NLR pair into an unlinked locus which allowed the new pair to evolve to respond to a new pathogen. This separation seems to provide plasticity to the system, as it allows the sensor NLRs to evolve more rapidly in response to the fast evolution of pathogen effectors whereas the helper NLR can evolve much slower to maintain its ability to induce HR. However, it seems that during evolution new helper NLRs also evolved, supposedly, because certain sensor NLRs require specific helper NLRs to function optimally. [20]

Bioinformatic analysis of plant NLRs has shown that there is a conserved MADA motif at the N-terminus of helper NLRs but not sensor NLRs. Around 20% of all CC-NLRs have the MADA motif, implying the motif's importance for the execution of HR. [21]

Regulation

Accidental activation of HR through the NLR proteins could cause vast destruction of the plant tissue, thus, the NLRs are kept in an inactive form through tight negative regulation at both transcriptional and post-translational levels. Under normal conditions, the mRNA of NLRs are transcribed at very low levels, which results in low levels of protein in the cell. The NLRs also require a considerable number of chaperone proteins for their folding. Misfolded proteins are immediately ubiquitinated and degraded by the proteasome. [22] It has been observed that in many cases, if the chaperone proteins involved in NLR biosynthesis are knocked-out, HR is abolished and NLR levels are significantly reduced. [23]

The domain structure of a typical plant NLR Plant NLR structure.png
The domain structure of a typical plant NLR

Intramolecular interactions are also essential for the regulation of HR. The NLR proteins are not linear: the NB-ARC domain is sandwiched in between the LRR and TIR/CC domains. Under normal conditions, there is a lot more ATP present in the cytoplasm than ADP, and this arrangement of the NLR proteins prevents the spontaneous exchange of ADP for ATP and thus activation of HR. Only when a virulence factor is sensed, the ADP is exchanged for ATP. [14]

Mutations in certain components of plant defence machinery result in HR being activated without the presence of pathogen effector proteins. Some of these mutations are observed in NLR genes and cause these NLR proteins to become auto-active due to disrupted intramolecular regulatory mechanisms. Other mutations causing spontaneous HR are present in proteins involved in ROS production during pathogen invasion. [3]

HR is also a temperature-sensitive process and it has been observed that in many cases plant-pathogen interactions do not induce HR at temperatures above 30 °C, which subsequently leads to increased susceptibility to the pathogen. [24] The mechanisms behind the influence of temperature on plant resistance to pathogens are not understood in detail, however, research suggests that the NLR protein levels might be important in this regulation. [25] It is also proposed that at higher temperatures the NLR proteins are less likely to form oligomeric complexes, thus inhibiting their ability to induce HR. [26]

It has also been shown that HR is dependent on the light conditions, which could be linked to the activity of chloroplasts and mainly their ability to generate ROS. [27]

Mediators

Several enzymes have been shown to be involved in generation of ROS. For example, copper amine oxidase, catalyzes the oxidative deamination of polyamines, especially putrescine, and releases the ROS mediators hydrogen peroxide and ammonia. [28] Other enzymes thought to play a role in ROS production include xanthine oxidase, NADPH oxidase, oxalate oxidase, peroxidases, and flavin containing amine oxidases. [9]

In some cases, the cells surrounding the lesion synthesize antimicrobial compounds, including phenolics, phytoalexins, and pathogenesis related (PR) proteins, including β-glucanases and chitinases. These compounds may act by puncturing bacterial cell walls; or by delaying maturation, disrupting metabolism, or preventing reproduction of the pathogen in question.

Studies have suggested that the actual mode and sequence of the dismantling of plant cellular components depends on each individual plant-pathogen interaction, but all HR seem to require the involvement of cysteine proteases. The induction of cell death and the clearance of pathogens also requires active protein synthesis, an intact actin cytoskeleton, and the presence of salicylic acid. [8]

Pathogen evasion

Pathogens have evolved several strategies to suppress plant defense responses. Host processes usually targeted by bacteria include; alterations to programmed cell death pathways, inhibiting cell wall-based defenses, and altering plant hormone signaling and expression of defense genes. [29]

Systemic immunity

Local initiation of HR in response to certain necrotrophic pathogens has been shown to allow the plants to develop systemic immunity against the pathogen. [30] Scientists have been trying to exploit the ability of HR to induce systemic resistance in plants in order to create transgenic plants resistant to certain pathogens. Pathogen-inducible promoters have been linked to auto-active NLR genes to induce HR response only when the pathogen is present but not at any other time. This approach, however, has been mostly unfeasible as the modification also leads to a substantial reduction in plant yields. [3]

Hypersensitive response as a driver for plant speciation

It has been noticed in Arabidopsis that sometimes when two different plant lines are crossed together, the offspring show signs of hybrid necrosis. This is due to the parent plants containing incompatible NLRs, which when expressed together in the same cell, induce spontaneous HR. [31]

This observation raised a hypothesis that plant pathogens can lead to the speciation of plants – if plant populations from the same species develop incompatible NLRs in response to different pathogen effectors, this can lead to hybrid necrosis in the F1 offspring, which substantially reduces the fitness of the offspring and gene flow to subsequent generations. [32]

Comparison to animal innate immunity

Both plants and animals have NLR proteins which seem to have the same biological function – to induce cell death. The N-termini of plant and animal NLRs vary but it seems that both have LRR domains at the C-terminus. [33]

A big difference between animal and plant NLRs is in what they recognise. Animal NLRs mainly recognise pathogen-associated molecular patterns (PAMPs), while plant NLRs mostly recognise pathogen effector proteins. This makes sense as NLRs are present inside of the cell and plants rarely have intracellular pathogens, except for viruses and viruses do not have PAMPs as they are rapidly evolving. Animals, on the other hand, have intracellular pathogens. [34]

The vast majority of plant lineages, except for certain algae, such as Chlamydomonas , have NLRs. NLRs are also present in many animal species, however, they are not present in, for example, Drosophila melanogaster and Arthropods. [33]

Upon recognition of PAMPs by NLRs in animals, the NLRs oligomerise to form a structure known as the inflammasome, which activates pyroptosis. In plants, structural studies have suggested that the NLRs also oligomerise to form a structure called the resistosome, which also leads to cell death. It seems that in both plants and animals, the formation of the resistosome or the inflammasome, respectively, leads to cell death by forming pores in the membrane. It is inferred from protein structures that in plants the NLRs themselves are responsible for forming pores in the membrane, while in the case of the inflammasome, the pore-forming activity arises from gasdermin D which is cleaved by caspases as a result of the oligomerisation of the NLRs. [35] [36] Plant cells do not have caspases. [37]

See also

Related Research Articles

<span class="mw-page-title-main">Immune system</span> Biological system protecting an organism against disease

The immune system is a network of biological systems that protects an organism from diseases. It detects and responds to a wide variety of pathogens, from viruses to parasitic worms, as well as cancer cells and objects such as wood splinters, distinguishing them from the organism's own healthy tissue. Many species have two major subsystems of the immune system. The innate immune system provides a preconfigured response to broad groups of situations and stimuli. The adaptive immune system provides a tailored response to each stimulus by learning to recognize molecules it has previously encountered. Both use molecules and cells to perform their functions.

<span class="mw-page-title-main">Calmodulin</span> Messenger protein

Calmodulin (CaM) (an abbreviation for calcium-modulated protein) is a multifunctional intermediate calcium-binding messenger protein expressed in all eukaryotic cells. It is an intracellular target of the secondary messenger Ca2+, and the binding of Ca2+ is required for the activation of calmodulin. Once bound to Ca2+, calmodulin acts as part of a calcium signal transduction pathway by modifying its interactions with various target proteins such as kinases or phosphatases.

Respiratory burst is the rapid release of the reactive oxygen species (ROS), superoxide anion and hydrogen peroxide, from different cell types.

<span class="mw-page-title-main">Adaptive immune system</span> Subsystem of the immune system

The adaptive immune system, also known as the acquired immune system, or specific immune system is a subsystem of the immune system that is composed of specialized, systemic cells and processes that eliminate pathogens or prevent their growth. The acquired immune system is one of the two main immunity strategies found in vertebrates.

Pattern recognition receptors (PRRs) play a crucial role in the proper function of the innate immune system. PRRs are germline-encoded host sensors, which detect molecules typical for the pathogens. They are proteins expressed mainly by cells of the innate immune system, such as dendritic cells, macrophages, monocytes, neutrophils, as well as by epithelial cells, to identify two classes of molecules: pathogen-associated molecular patterns (PAMPs), which are associated with microbial pathogens, and damage-associated molecular patterns (DAMPs), which are associated with components of host's cells that are released during cell damage or death. They are also called primitive pattern recognition receptors because they evolved before other parts of the immune system, particularly before adaptive immunity. PRRs also mediate the initiation of antigen-specific adaptive immune response and release of inflammatory cytokines.

<span class="mw-page-title-main">Innate immune system</span> Immunity strategy in living beings

The innate, or nonspecific, immune system is one of the two main immunity strategies in vertebrates. The innate immune system is an alternate defense strategy and is the dominant immune system response found in plants, fungi, prokaryotes, and invertebrates.

Systemic acquired resistance (SAR) is a "whole-plant" resistance response that occurs following an earlier localized exposure to a pathogen. SAR is analogous to the innate immune system found in animals, and although there are many shared aspects between the two systems, it is thought to be a result of convergent evolution. The systemic acquired resistance response is dependent on the plant hormone, salicylic acid.

<span class="mw-page-title-main">Caspase 1</span> Protein-coding gene in the species Homo sapiens

Caspase-1/Interleukin-1 converting enzyme (ICE) is an evolutionarily conserved enzyme that proteolytically cleaves other proteins, such as the precursors of the inflammatory cytokines interleukin 1β and interleukin 18 as well as the pyroptosis inducer Gasdermin D, into active mature peptides. It plays a central role in cell immunity as an inflammatory response initiator. Once activated through formation of an inflammasome complex, it initiates a proinflammatory response through the cleavage and thus activation of the two inflammatory cytokines, interleukin 1β (IL-1β) and interleukin 18 (IL-18) as well as pyroptosis, a programmed lytic cell death pathway, through cleavage of Gasdermin D. The two inflammatory cytokines activated by Caspase-1 are excreted from the cell to further induce the inflammatory response in neighboring cells.

The gene-for-gene relationship is a concept in plant pathology that plants and their diseases each have single genes that interact with each other during an infection. It was proposed by Harold Henry Flor who was working with rust (Melampsora lini) of flax (Linum usitatissimum). Flor showed that the inheritance of both resistance in the host and parasite ability to cause disease is controlled by pairs of matching genes. One is a plant gene called the resistance (R) gene. The other is a parasite gene called the avirulence (Avr) gene. Plants producing a specific R gene product are resistant towards a pathogen that produces the corresponding Avr gene product. Gene-for-gene relationships are a widespread and very important aspect of plant disease resistance. Another example can be seen with Lactuca serriola versus Bremia lactucae.

Pyroptosis is a highly inflammatory form of lytic programmed cell death that occurs most frequently upon infection with intracellular pathogens and is likely to form part of the antimicrobial response. This process promotes the rapid clearance of various bacterial, viral, fungal and protozoan infections by removing intracellular replication niches and enhancing the host's defensive responses. Pyroptosis can take place in immune cells and is also reported to occur in keratinocytes and some epithelial cells.

<span class="mw-page-title-main">NLRP1</span> Human protein-coding gene

NLRP1 encodes NACHT, LRR, FIIND, CARD domain and PYD domains-containing protein 1 in humans. NLRP1 was the first protein shown to form an inflammasome. NLRP1 is expressed by a variety of cell types, which are predominantly epithelial or hematopoietic. The expression is also seen within glandular epithelial structures including the lining of the small intestine, stomach, airway epithelia and in hairless or glabrous skin. NLRP1 polymorphisms are associated with skin extra-intestinal manifestations in CD. Its highest expression was detected in human skin, in psoriasis and in vitiligo. Polymorphisms of NLRP1 were found in lupus erythematosus and diabetes type 1. Variants of mouse NLRP1 were found to be activated upon N-terminal cleavage by the protease in anthrax lethal factor.

<span class="mw-page-title-main">NLRP2</span> Protein-coding gene in the species Homo sapiens

NACHT, LRR and PYD domains-containing protein 2 is a protein that in humans is encoded by the NLRP2 gene.

<span class="mw-page-title-main">NOD-like receptor</span> Class of proteins

The nucleotide-binding oligomerization domain-like receptors, or NOD-like receptors (NLRs), are intracellular sensors of pathogen-associated molecular patterns (PAMPs) that enter the cell via phagocytosis or pores, and damage-associated molecular patterns (DAMPs) that are associated with cell stress. They are types of pattern recognition receptors (PRRs), and play key roles in the regulation of innate immune response. NLRs can cooperate with toll-like receptors (TLRs) and regulate inflammatory and apoptotic response.

<span class="mw-page-title-main">Inflammasome</span> Cytosolic multiprotein complex that mediates the activation of Caspase 1

Inflammasomes are cytosolic multiprotein oligomers of the innate immune system responsible for the activation of inflammatory responses. Activation and assembly of the inflammasome promotes proteolytic cleavage, maturation and secretion of pro-inflammatory cytokines interleukin 1β (IL-1β) and interleukin 18 (IL-18), as well as cleavage of gasdermin D. The N-terminal fragment resulting from this cleavage induces a pro-inflammatory form of programmed cell death distinct from apoptosis, referred to as pyroptosis, and is responsible for secretion of the mature cytokines, presumably through the formation of pores in the plasma membrane. Additionally, inflammasomes can be incorporated into larger cell death-inducing complexes called PANoptosomes, which drive another distinct form of pro-inflammatory cell death called PANoptosis.

Resistance genes (R-Genes) are genes in plant genomes that convey plant disease resistance against pathogens by producing R proteins. The main class of R-genes consist of a nucleotide binding domain (NB) and a leucine rich repeat (LRR) domain(s) and are often referred to as (NB-LRR) R-genes or NLRs. Generally, the NB domain binds either ATP/ADP or GTP/GDP. The LRR domain is often involved in protein-protein interactions as well as ligand binding. NB-LRR R-genes can be further subdivided into toll interleukin 1 receptor (TIR-NB-LRR) and coiled-coil (CC-NB-LRR).

Biotic stress is stress that occurs as a result of damage done to an organism by other living organisms, such as bacteria, viruses, fungi, parasites, beneficial and harmful insects, weeds, and cultivated or native plants. It is different from abiotic stress, which is the negative impact of non-living factors on the organisms such as temperature, sunlight, wind, salinity, flooding and drought. The types of biotic stresses imposed on an organism depend the climate where it lives as well as the species' ability to resist particular stresses. Biotic stress remains a broadly defined term and those who study it face many challenges, such as the greater difficulty in controlling biotic stresses in an experimental context compared to abiotic stress.

<span class="mw-page-title-main">Plant disease resistance</span> Ability of a plant to stand up to trouble

Plant disease resistance protects plants from pathogens in two ways: by pre-formed structures and chemicals, and by infection-induced responses of the immune system. Relative to a susceptible plant, disease resistance is the reduction of pathogen growth on or in the plant, while the term disease tolerance describes plants that exhibit little disease damage despite substantial pathogen levels. Disease outcome is determined by the three-way interaction of the pathogen, the plant and the environmental conditions.

<span class="mw-page-title-main">NLRP11</span> Protein-coding gene in the species Homo sapiens

NOD-like receptor family pyrin domain containing 11 is a protein that in humans is encoded by the NLRP11 gene located on the long arm of human chromosome 19q13.42. NLRP11 belongs to the NALP subfamily, part of a large subfamily of CATERPILLER. It is also known as NALP11, PYPAF6, NOD17, PAN10, and CLR19.6

Guard theory is a branch of immunology which concerns the innate sensing of stereotypical consequences of a virulence factor or pathogen. This is in contrast to the classical understanding of recognition by the innate immune system, which involves recognition of distinct microbial structures- pathogen-associated molecular patterns (PAMPs)- with pattern recognition receptors (PRRs). Some of these stereotypical consequences of virulence factors and pathogens may include altered endosomal trafficking and changes in the cytoskeleton. These recognition mechanisms would work to complement classical pattern recognition mechanisms.

<span class="mw-page-title-main">Leucine-rich repeat receptor like protein kinase</span>

Leucine-rich repeat receptor like protein kinase are plant cell membrane localized Leucine-rich repeat (LRR) receptor kinase that play critical roles in plant innate immunity. Plants have evolved intricate immunity mechanism to combat against pathogen infection by recognizing Pathogen Associated Molecular Patterns (PAMP) and endogenous Damage Associated Molecular Patterns (DAMP). PEPR 1 considered as the first known DAMP receptor of Arabidopsis.

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