The type III secretion system (T3SS or TTSS) is one of the bacterial secretion systems used by bacteria to secrete their effector proteins into the host's cells to promote virulence and colonisation.[1][2] While the type III secretion system has been widely regarded as equivalent to the injectisome, many argue that the injectisome is only part of the type III secretion system, which also include structures like the flagellar export apparatus.[3] The T3SS is a needle-like protein complex found in several species of pathogenicgram-negative bacteria.
The term Type III secretion system was coined in 1993.[4] This secretion system is distinguished from at least five other secretion systems found in gram-negative bacteria. Many animal and plant associated bacteria possess similar T3SSs. These T3SSs are similar as a result of convergent evolution and phylogenetic analysis supports a model in which gram-negative bacteria can transfer the T3SS gene cassettehorizontally to other species. Some of the most researched T3SSs are from species of:[citation needed]
The T3SS is composed of approximately 30 different proteins, making it one of the most complex secretion systems. Its structure shows many similarities with bacterial flagella (long, rigid, extracellular structures used for motility). Some of the proteins participating in T3SS share amino-acid sequence homology to flagellar proteins. Some of the bacteria possessing a T3SS have flagella as well and are motile (Salmonella, for instance), and some do not (Shigella, for instance). Technically speaking, type III secretion is used both for secreting infection-related proteins and flagellar components. However, the term "type III secretion" is used mainly in relation to the infection apparatus. The bacterial flagellum shares a common ancestor with the type III secretion system.[5][6]
T3SSs are essential for the pathogenicity (the ability to infect) of many pathogenic bacteria. Defects in the T3SS may render a bacterium non-pathogenic. It has been suggested that some non-invasive strains of gram-negative bacteria have lost the T3SS because the energetically costly system is no longer of use.[7] Although traditional antibiotics were effective against these bacteria in the past, antibiotic-resistant strains constantly emerge. Understanding the way the T3SS works and developing drugs targeting it specifically have become an important goal of many research groups around the world since the late 1990s.
The hallmark of T3SS is the needle[8][9] (more generally, the needle complex (NC) or the T3SS apparatus (T3SA); also called injectisome when the ATPase is excluded; see below). Bacterial proteins that need to be secreted pass from the bacterial cytoplasm through the needle directly into the host cytoplasm. Three membranes separate the two cytoplasms: the double membranes (inner and outer membranes) of the Gram-negative bacterium and the eukaryotic membrane. The needle provides a smooth passage through those highly selective and almost impermeable membranes. A single bacterium can have several hundred needle complexes spread across its membrane. It has been proposed that the needle complex is a universal feature of all T3SSs of pathogenic bacteria.[10]
The needle complex starts at the cytoplasm of the bacterium, crosses the two membranes and protrudes from the cell. The part anchored in the membrane is the base (or basal body) of the T3SS. The extracellular part is the needle. A so-called inner rod connects the needle to the base. The needle itself, although the biggest and most prominent part of the T3SS, is made out of many units of a single protein. The majority of the different T3SS proteins are therefore those that build the base and those that are secreted into the host. As mentioned above, the needle complex shares similarities with bacterial flagella. More specifically, the base of the needle complex is structurally very similar to the flagellar base; the needle itself is analogous to the flagellar hook, a structure connecting the base to the flagellar filament.[11][12]
The base is composed of several circular rings and is the first structure that is built in a new needle complex. Once the base is completed, it serves as a secretion machine for the outer proteins (the needle). Once the whole complex is completed the system switches to secreting proteins that are intended to be delivered into host cells. The needle is presumed to be built from bottom to top; units of needle monomer protein pile upon each other, so that the unit at the tip of the needle is the last one added. The needle subunit is one of the smallest T3SS proteins, measuring at around 9 kDa. 100−150 subunits comprise each needle.
The T3SS needle measures around 60−80 nm in length and 8nm in external width. It needs to have a minimal length so that other extracellular bacterial structures (adhesins and the lipopolysaccharide layer, for instance) do not interfere with secretion. The hole of the needle has a 3nm diameter. Most folded effector proteins are too large to pass through the needle opening, so most secreted proteins must pass through the needle unfolded, a task carried out by the ATPase at the base of the structure.[13]
T3SS proteins
Diagram of individual substructures of the needle complex from Salmonella typhimurium
The T3SS proteins can be grouped into three categories:
Structural proteins: build the base, the inner rod and the needle.
Effector proteins: get secreted into the host cell and promote infection / suppress host cell defences.
Chaperones: bind effectors in the bacterial cytoplasm, protect them from aggregation and degradation and direct them towards the needle complex.
Most T3SS genes are laid out in operons. These operons are located on the bacterial chromosome in some species and on a dedicated plasmid in other species. Salmonella, for instance, has a chromosomal region in which most T3SS genes are gathered, the so-called Salmonellapathogenicity island (SPI). Shigella, on the other hand, has a large virulence plasmid on which all T3SS genes reside. It is important to note that many pathogenicity islands and plasmids contain elements that allow for frequent horizontal gene transfer of the island/plasmid to a new species.
Effector proteins that are to be secreted through the needle need to be recognized by the system, since they float in the cytoplasm together with thousands of other proteins. Recognition is done through a secretion signal—a short sequence of amino acids located at the beginning (the N-terminus) of the protein (usually within the first 20 amino acids), that the needle complex is able to recognize. Unlike other secretion systems, the secretion signal of T3SS proteins is never cleaved off the protein.
Induction of secretion
Contact of the needle with a host cell triggers the T3SS to start secreting;[14] not much is known about this trigger mechanism (see below). Secretion can also be induced by lowering the concentration of calciumions in the growth medium (for Yersinia and Pseudomonas; done by adding a chelator such as EDTA or EGTA) and by adding the aromaticdyeCongo red to the growth medium (for Shigella), for instance. These methods and other are used in laboratories to artificially induce type III secretion.
Induction of secretion by external cues other than contact with host cells also takes place in vivo, in infected organisms. The bacteria sense such cues as temperature, pH, osmolarity and oxygen levels, and use them to "decide" whether to activate their T3SS. For instance, Salmonella can replicate and invade better in the ileum rather than in the cecum of animal intestine. The bacteria are able to know where they are thanks to the different ions present in these regions; the ileum contains formate and acetate, while the cecum does not. The bacteria sense these molecules, determine that they are at the ileum and activate their secretion machinery. Molecules present in the cecum, such as propionate and butyrate, provide a negative cue to the bacteria and inhibit secretion. Cholesterol, a lipid found in most eukaryotic cell membranes, is able to induce secretion in Shigella.
The external cues listed above either regulate secretion directly or through a genetic mechanism. Several transcription factors that regulate the expression of T3SS genes are known. Some of the chaperones that bind T3SS effectors also act as transcription factors. A feedback mechanism has been suggested: when the bacterium does not secrete, its effector proteins are bound to chaperones and float in the cytoplasm. When secretion starts, the chaperones detach from the effectors and the latter are secreted and leave the cell. The lone chaperones then act as transcription factors, binding to the genes encoding their effectors and inducing their transcription and thereby the production of more effectors.
Structures similar to Type3SS injectisomes have been proposed to rivet gram negative bacterial outer and inner membranes to help release outer membrane vesicles targeted to deliver bacterial secretions to eukaryotic host or other target cells in vivo.[15]
T3SS-mediated infection
T3SS effectors enter the needle complex at the base and make their way inside the needle towards the host cell. The exact way in which effectors enter the host is mostly unknown. It has been previously suggested that the needle itself is capable of puncturing a hole in the host cell membrane; this theory has been refuted. It is now clear that some effectors, collectively named translocators, are secreted first and produce a pore or a channel (a translocon) in the host cell membrane, through which other effectors may enter. Mutated bacteria that lack translocators are able to secrete proteins but are not able to deliver them into host cells. In general each T3SS includes three translocators. Some translocators serve a double role; after they participate in pore formation they enter the cell and act as bona fide effectors.
T3SS effectors manipulate host cells in several ways. The most striking effect is the promoting of uptake of the bacterium by the host cell. Many bacteria possessing T3SSs must enter host cells in order to replicate and propagate infection. The effectors they inject into the host cell induce the host to engulf the bacterium and to practically "eat" it. In order for this to happen the bacterial effectors manipulate the actinpolymerization machinery of the host cell. Actin is a component of the cytoskeleton and it also participates in motility and in changes in cell shape. Through its T3SS effectors the bacterium is able to utilize the host cell's own machinery for its own benefit. Once the bacterium has entered the cell it is able to secrete other effectors more easily and it can penetrate neighboring cells and quickly infect the whole tissue.
T3SS effectors have also been shown to tamper with the host's cell cycle and some of them are able to induce apoptosis. One of the most researched T3SS effector is IpaB from Shigella flexneri. It serves a double role, both as a translocator, creating a pore in the host cell membrane, and as an effector, exerting multiple detrimental effects on the host cell. It had been demonstrated that IpaB induces apoptosis in macrophages—cells of the animal immune system—after being engulfed by them.[16] It was later shown that IpaB achieves this by interacting with caspase 1, a major regulatory protein in eukaryotic cells.[17]
Another well characterized class of T3SS effectors are Transcription Activator-like effectors (TAL effectors) from Xanthomonas. When injected into plants, these proteins can enter the nucleus of the plant cell, bind plant promoter sequences, and activate transcription of plant genes that aid in bacterial infection.[18] TAL effector-DNA recognition has recently been demonstrated to comprise a simple code[19][20] and this has greatly improved the understanding of how these proteins can alter the transcription of genes in the host plant cells.
Unresolved issues
The topology and organization of the Salmonella needle complex.
Hundreds of articles on T3SS have been published since the mid-nineties. However, numerous issues regarding the system remain unresolved:
T3SS proteins. Of the approximately 30 T3SS proteins less than 10 in each organism have been directly detected using biochemical methods. The rest, being perhaps rare, have proven difficult to detect and they remain theoretical (although genetic rather than biochemical studies have been performed on many T3SS genes/proteins). The localization of each protein is also not entirely known.
The length of the needle. It is not known how the bacterium "knows" when a new needle has reached its proper length. Several theories exist, among them the existence of a "ruler protein" that somehow connects the tip and the base of the needle. Addition of new monomers to the tip of the needle should stretch the ruler protein and thereby signal the needle length to the base.
Energetics. The force that drives the passage of proteins inside the needle is not completely known. An ATPase is associated with the base of the T3SS and participates in directing proteins into the needle; but whether it supplies the energy for transport is not clear.
Secretion signal. As mentioned above, the existence of a secretion signal in effector proteins is known. The signal allows the system to distinguish T3SS-transported proteins from any other protein. Its nature, requirements and the mechanism of recognition are poorly understood, but methods for predicting which bacterial proteins can be transported by the Type III secretion system have recently been developed.[22]
Activation of secretion. The bacterium must know when the time is right to secrete effectors. Unnecessary secretion, when no host cell is in vicinity, is wasteful for the bacterium in terms of energy and resources. The bacterium is somehow able to recognize contact of the needle with the host cell. How this is done is still being researched, and the method may well be dependent on the pathogen. Some theories postulate a delicate conformational change in the structure of the needle upon contact with the host cell; this change perhaps serves as a signal for the base to commence secretion. One method of recognition has been discovered in Salmonella, which relies on sensing host cell cytosolic pH through the pathogenicity island 2-encoded T3SS in order to switch on secretion of effectors.[23]
Binding of chaperones. It is not known when chaperones bind their effectors (whether during or after translation) and how they dissociate from their effectors before secretion.
Effector mechanisms. Although much was revealed since the beginning of the 21st century about the ways in which T3SS effectors manipulate the host, the majority of effects and pathways remains unknown.
Evolution. As mentioned, the T3SS is closely related to the bacterial flagellum.[24] There are three competing hypotheses:[25] first, that the flagellum evolved first and the T3SS is derived from that structure, second, that the T3SS evolved first and the flagellum is derived from it, and third, that the two structures are derived from a common ancestor. There was some controversy about the different scenarios,[5][25] since they all explain protein homology between the two structures, as well as their functional diversity.[26] Yet, recent phylogenomic evidence favours the hypothesis that the T3SS derived from the flagellum by a process involving initial gene loss and then gene acquisition.[27] A key step of the latter process was the recruitment of secretins to the T3SS, an event that occurred at least three times from other membrane-associated systems.
Nomenclature of T3SS proteins
Flagellum of Gram-negative bacteria. The rings of the base are very similar to needle-complex rings, although the existence of a C-ring in the needle complex has not been proven. The flagellar hook is homologous to the T3SS needle
Since the beginning of the 1990s new T3SS proteins are being found in different bacterial species at a steady rate. Abbreviations have been given independently for each series of proteins in each organism, and the names usually do not reveal much about the protein's function. Some proteins discovered independently in different bacteria have later been shown to be homologous; the historical names, however, have mostly been kept, a fact that might cause confusion. For example, the proteins SicA, IpgC and SycD are homologs from Salmonella, Shigella and Yersinia, respectively, but the last letter (the "serial number") in their name does not show that.
Below is a summary of the most common protein-series names in several T3SS-containing species. Note that these names include proteins that form the T3SS machinery as well as the secreted effector proteins:
Following those abbreviations is a letter or a number. Letters usually denote a "serial number", either the chronological order of discovery or the physical order of appearance of the gene in an operon. Numbers, the rarer case, denote the molecular weight of the protein in kDa. Examples: IpaA, IpaB, IpaC; MxiH, MxiG, MxiM; Spa9, Spa47.
Several key elements appear in all T3SSs: the needle monomer, the inner rod of the needle, the ring proteins, the two translocators, the needle-tip protein, the ruler protein (which is thought to determine the needle's length; see above) and the ATPase, which supplies energy for secretion. The following table shows some of these key proteins in four T3SS-containing bacteria:
↓ Function / Genus →
Shigella
Salmonella
Yersinia
Escherichia
Needle monomer
MxiH
PrgI
YscF
EscF
Inner rod
MxiI
PrgJ
YscI
EscI
Needle-tip protein
IpaD
SipD
LcrV
EspA
Translocator
IpaB
SipB
YopB
EspD
Translocator
IpaC
SipC
YopD
EspB
Chaperone for the two translocators
IpgC
SicA
SycD
CesD
ATPase
Spa47
InvC
YscN
SepB (EscN)
Ruler protein
Spa32
InvJ
YscP
Orf16
Switch
Spa40
SpaS
YscU
EscU
Gatekeeper
MxiC
InvE
YopN (TyeA)
SepL
Methods employed in T3SS research
Isolation of T3SS needle complexes
The isolation of large, fragile, hydrophobic membrane structures from cells has constituted a challenge for many years. By the end of the 1990s, however, several approaches have been developed for the isolation of T3SS NCs. In 1998 the first NCs were isolated from Salmonella typhimurium.[29]
For the isolation, bacteria are grown in a large volume of liquid growth medium until they reach log phase. They are then centrifuged; the supernatant (the medium) is discarded and the pellet (the bacteria) is resuspended in a lysis buffer typically containing lysozyme and sometimes a detergent such as LDAO or Triton X-100. This buffer disintegrates the cell wall. After several rounds of lysis and washing, the opened bacteria are subjected to a series of ultracentrifugations. This treatment enriches large macromolecular structures and discards smaller cell components. Optionally, the final lysate is subjected to further purification by CsCldensity gradient.
An additional approach for further purification uses affinity chromatography. Recombinant T3SS proteins that carry a protein tag (a histidine tag, for instance) are produced by molecular cloning and then introduced (transformed) into the researched bacteria. After initial NC isolation, as described above, the lysate is passed through a column coated with particles with high affinity to the tag (in the case of histidine tags: nickelions). The tagged protein is retained in the column, and with it the entire needle complex. High degrees of purity can be achieved using such methods. This purity is essential for many delicate assays that have been used for NC characterization.
Type III effectors were known since the beginning of the 1990s, but the way in which they are delivered into host cells was a complete mystery. The homology between many flagellar and T3SS proteins led researchers to suspects the existence of an outer T3SS structure similar to flagella. The identification and subsequent isolation of the needle structure enabled researchers to:
characterize the three-dimensional structure of the NC in detail, and through this to draw conclusions regarding the mechanism of secretion (for example, that the narrow width of the needle requires unfolding of effectors prior to secretion),
analyze the protein components of the NC, this by subjecting isolated needles to proteomic analysis (see below),
assign roles to various NC components, this by knocking out T3SS genes, isolating NCs from the mutated bacteria and examining the changes that the mutations caused.
Microscopy, crystallography and solid-state NMR
As with almost all proteins, the visualization of T3SS NCs is only possible with electron microscopy. The first images of NCs (1998) showed needle structures protruding from the cell wall of live bacteria and flat, two-dimensional isolated NCs.[29] In 2001 images of NCs from Shigella flexneri were digitally analyzed and averaged to obtain a first semi-3D structure of the NC.[8] The helical structure of NCs from Shigella flexneri was resolved at a resolution of 16 Å using X-rayfiber diffraction in 2003,[30] and a year later a 17-Å 3D structure of NCs from Salmonella typhimurium was published.[31] Recent advances and approaches have allowed high-resolution 3D images of the NC,[32][33] further clarifying the complex structure of the NC.
Numerous T3SS proteins have been crystallized over the years. These include structural proteins of the NC, effectors and chaperones. The first structure of a needle-complex monomer was NMR structure of BsaL from "Burkholderia pseudomallei" and later the crystal structure of MixH from Shigella flexneri, which were both resolved in 2006.[34][35]
In 2012, a combination of recombinant wild-type needle production, solid-state NMR, electron microscopy[36] and Rosetta modeling revealed the supramolecular interfaces and ultimately the complete atomic structure of the Salmonella typhimurium T3SS needle.[37] It was shown that the 80-residue PrgI subunits form a right-handed helical assembly with roughly 11 subunits per two turns, similar to that of the flagellum of Salmonella typhimurium. The model also revealed an extended amino-terminal domain that is positioned on the surface of the needle, while the highly conserved carboxy terminus points towards the lumen.[37]
Proteomics
Several methods have been employed in order to identify the array of proteins that comprise the T3SS. Isolated needle complexes can be separated with SDS-PAGE. The bands that appear after staining can be individually excised from the gel and analyzed using protein sequencing and mass spectrometry. The structural components of the NC can be separated from each other (the needle part from the base part, for instance), and by analyzing those fractions the proteins participating in each one can be deduced. Alternatively, isolated NCs can be directly analyzed by mass spectrometry, without prior electrophoresis, in order to obtain a complete picture of the NC proteome.
Genetic and functional studies
The T3SS in many bacteria has been manipulated by researchers. Observing the influence of individual manipulations can be used to draw insights into the role of each component of the system. Examples of manipulations are:
Overexpression of one or more T3SS genes (in other words: production in vivo of a T3SS protein in quantities larger than usual).
Point or regional changes in T3SS genes or proteins. This is done in order to define the function of specific amino acids or regions in a protein.
The introduction of a gene or a protein from one species of bacteria into another (cross-complementation assay). This is done in order to check for differences and similarities between two T3SSs.
Manipulation of T3SS components can have influence on several aspects of bacterial function and pathogenicity. Examples of possible influences:
The ability of the bacteria to invade host cells, in the case of intracellular pathogens. This can be measured using an invasion assay (gentamicin protection assay).
The ability of intracellular bacteria to migrate between host cells.
The ability of the bacteria to kill host cells. This can be measured by several methods, for instance by the LDH-release assay, in which the enzyme LDH, which leaks from dead cells, is identified by measuring its enzymatic activity.
The ability of a T3SS to secrete a specific protein or to secrete at all. In order to assay this, secretion is induced in bacteria growing in liquid medium. The bacteria and medium are then separated by centrifugation, and the medium fraction (the supernatant) is then assayed for the presence of secreted proteins. In order to prevent a normally secreted protein from being secreted, a large molecule can be artificially attached to it. If the then non-secreted protein stays "stuck" at the bottom of the needle complex, the secretion is effectively blocked.
The ability of the bacteria to assemble an intact needle complex. NCs can be isolated from manipulated bacteria and examined microscopically. Minor changes, however cannot always be detected by microscopy.
The ability of bacteria to infect live animals or plants. Even if manipulated bacteria are shown in vitro to be able to infect host cells, their ability to sustain an infection in a live organism cannot be taken for granted.
The expression levels of other genes. This can be assayed in several ways, notably northern blot and RT-PCR. The expression levels of the entire genome can be assayed by microarray. Many type III transcription factors and regulatory networks were discovered using these methods.
The growth and fitness of bacteria.
Inhibitors of the T3SS
A few compounds have been discovered that inhibit the T3SS in gram-negative bacteria, including the guadinomines which are naturally produced by Streptomyces species.[38]Monoclonal antibodies have been developed that inhibit the T3SS too.[39] Aurodox, an antibiotic capable of inhibiting the translation of T3SS proteins has been shown to able to prevent T3SS effectors in vitro and in animal models[40][41]
A flagellum is a hairlike appendage that protrudes from certain plant and animal sperm cells, from fungal spores (zoospores), and from a wide range of microorganisms to provide motility. Many protists with flagella are known as flagellates.
Shigella is a genus of bacteria that is Gram-negative, facultatively anaerobic, non–spore-forming, nonmotile, rod-shaped, and is genetically closely related to Escherichia. The genus is named after Kiyoshi Shiga, who discovered it in 1897.
An exotoxin is a toxin secreted by bacteria. An exotoxin can cause damage to the host by destroying cells or disrupting normal cellular metabolism. They are highly potent and can cause major damage to the host. Exotoxins may be secreted, or, similar to endotoxins, may be released during lysis of the cell. Gram negative pathogens may secrete outer membrane vesicles containing lipopolysaccharide endotoxin and some virulence proteins in the bounding membrane along with some other toxins as intra-vesicular contents, thus adding a previously unforeseen dimension to the well-known eukaryote process of membrane vesicle trafficking, which is quite active at the host–pathogen interface.
Flagellin is a globular protein that arranges itself in a hollow cylinder to form the filament in a bacterial flagellum. It has a mass of about 30,000 to 60,000 daltons. Flagellin is the principal component of bacterial flagella, and is present in large amounts on nearly all flagellated bacteria.
Secretion is the movement of material from one point to another, such as a secreted chemical substance from a cell or gland. In contrast, excretion is the removal of certain substances or waste products from a cell or organism. The classical mechanism of cell secretion is via secretory portals at the plasma membrane called porosomes. Porosomes are permanent cup-shaped lipoprotein structures embedded in the cell membrane, where secretory vesicles transiently dock and fuse to release intra-vesicular contents from the cell.
Shigella flexneri is a species of Gram-negative bacteria in the genus Shigella that can cause diarrhea in humans. Several different serogroups of Shigella are described; S. flexneri belongs to group B. S. flexneri infections can usually be treated with antibiotics, although some strains have become resistant. Less severe cases are not usually treated because they become more resistant in the future. Shigella are closely related to Escherichia coli, but can be differentiated from E.coli based on pathogenicity, physiology and serology.
Yersinia pseudotuberculosis is a Gram-negative bacterium that causes Far East scarlet-like fever in humans, who occasionally get infected zoonotically, most often through the food-borne route. Animals are also infected by Y. pseudotuberculosis. The bacterium is urease positive.
Rhizopus microsporus is a fungal plant pathogen infecting maize, sunflower, and rice.
In molecular biology, LcrV is a protein found in Yersinia pestis and several other bacterial species. It forms part of the Yersinia pestis virulence protein factors that also includes all Yops, or Yersinia outer protein, but the name has been kept out of convention. LcrV's main function is not actually known, but it is essential for the production of other Yops.
Omptins are a family of bacterial proteases. They are aspartate proteases, which cleave peptides with the use of a water molecule. Found in the outer membrane of gram-negative enterobacteria such as Shigella flexneri, Yersinia pestis, Escherichia coli, and Salmonella enterica. Omptins consist of a widely conserved beta barrel spanning the membrane with 5 extracellular loops. These loops are responsible for the various substrate specificities. These proteases rely upon binding of lipopolysaccharide for activity.
In molecular biology, YopR is a protein domain commonly found in gram negative bacteria, in particular Yersinia and is a core domain. Proteins in this entry are type III secretion system effectors. They are named differently in different species and in Yersinia has been designated YopR which is encoded by the YscH gene. This Yop protein is unusual in that it is released to the extracellular environment rather than injected directly into the target cell as are most Yop proteins. A hallmark of Yersinia type III machines is the presence of needles extending from the bacterial surface. Needles perform two functions, firstly, as a channel to export effectors into the immune cells and secondly as a sensor.
Jorge Enrique Galán is an Argentinian-American microbiologist who specializes in infectious disease, bacterial pathogenesis including Salmonella.
Bacterial effectors are proteins secreted by pathogenic bacteria into the cells of their host, usually using a type 3 secretion system (TTSS/T3SS), a type 4 secretion system (TFSS/T4SS) or a Type VI secretion system (T6SS). Some bacteria inject only a few effectors into their host’s cells while others may inject dozens or even hundreds. Effector proteins may have many different activities, but usually help the pathogen to invade host tissue, suppress its immune system, or otherwise help the pathogen to survive. Effector proteins are usually critical for virulence. For instance, in the causative agent of plague, the loss of the T3SS is sufficient to render the bacteria completely avirulent, even when they are directly introduced into the bloodstream. Gram negative microbes are also suspected to deploy bacterial outer membrane vesicles to translocate effector proteins and virulence factors via a membrane vesicle trafficking secretory pathway, in order to modify their environment or attack/invade target cells, for example, at the host-pathogen interface.
Membrane vesicle trafficking in eukaryotic animal cells involves movement of biochemical signal molecules from synthesis-and-packaging locations in the Golgi body to specific release locations on the inside of the plasma membrane of the secretory cell. It takes place in the form of Golgi membrane-bound micro-sized vesicles, termed membrane vesicles (MVs).
The type VI secretion system (T6SS) is molecular machine used by a wide range of Gram-negative bacterial species to transport effectors from the interior of a bacterial cell across the cellular envelope into an adjacent target cell. While often reported that the T6SS was discovered in 2006 by researchers studying the causative agent of cholera, Vibrio cholerae, the first study demonstrating that T6SS genes encode a protein export apparatus was actually published in 2004, in a study of protein secretion by the fish pathogen Edwardsiella tarda.
The type 2 secretion system is a type of protein secretion machinery found in various species of Gram-negative bacteria, including many human pathogens such as Pseudomonas aeruginosa and Vibrio cholerae. The type II secretion system is one of six protein secretory systems commonly found in Gram-negative bacteria, along with the type I, type III, and type IV secretion systems, as well as the chaperone/usher pathway, the autotransporter pathway/type V secretion system, and the type VI secretion system. Like these other systems, the type II secretion system enables the transport of cytoplasmic proteins across the lipid bilayers that make up the cell membranes of Gram-negative bacteria. Secretion of proteins and effector molecules out of the cell plays a critical role in signaling other cells and in the invasion and parasitism of host cells.
Contact-dependent growth inhibition (CDI) is a phenomenon where a bacterial cell may deliver a polymorphic toxin molecule into neighbouring bacterial cells upon direct cell-cell contact, causing growth arrest or cell death.
Bacterial secretion systems are protein complexes present on the cell membranes of bacteria for secretion of substances. Specifically, they are the cellular devices used by pathogenic bacteria to secrete their virulence factors to invade the host cells. They can be classified into different types based on their specific structure, composition and activity. Generally, proteins can be secreted through two different processes. One process is a one-step mechanism in which proteins from the cytoplasm of bacteria are transported and delivered directly through the cell membrane into the host cell. Another involves a two-step activity in which the proteins are first transported out of the inner cell membrane, then deposited in the periplasm, and finally through the outer cell membrane into the host cell.
Nucleomodulins are a family of bacterial proteins that enter the nucleus of eukaryotic cells.
Type VII secretion systems are bacterial secretion systems first observed in the phyla Actinomycetota and Bacillota. Bacteria use such systems to transport, or secrete, proteins into the environment. The bacterial genus Mycobacterium uses type VII secretion systems (T7SS) to secrete proteins across their cell envelope. The first T7SS system discovered was the ESX-1 System.
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1 2 Kubori T, Matsushima Y, Nakamura D, Uralil J, Lara-Tejero M, Sukhan A, etal. (April 1998). "Supramolecular structure of the Salmonella typhimurium type III protein secretion system". Science. 280 (5363): 602–605. Bibcode:1998Sci...280..602K. doi:10.1126/science.280.5363.602. PMID9554854.
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Host-Pathogen Interaction in Pseudomonas syringae pv. tomato and tomato plant leading to bacterial speck disease.
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