Jennifer S. Brodbelt

Last updated
Jennifer Sue Brodbelt
Alma mater Purdue University
Known for Mass Spectrometry
AwardsField and Franklin Award
Scientific career
Fields Analytical Chemistry, Mass Spectrometry
Institutions Purdue University, University of California, Santa Barbara, University of Texas at Austin
Thesis Aspects of ion chemistry in mass spectrometry  (1988)
Doctoral advisor R. Graham Cooks
Website http://brodbelt.cm.utexas.edu/research/

Jennifer S. Brodbelt is an American chemist known for her research using mass spectrometry to characterize organic compounds, especially biopolymers and proteins.

Contents

Education and career

Brodbelt has an undergraduate degree from the University of Virginia and earned her Ph.D. from Purdue University where she worked on gas phase ion chemistry using mass spectrometry. [1] Following her Ph.D. she was a postdoc at the University of California, Santa Barbara [2] before joining the University of Texas at Austin in 1989. [3] As of 2016, she is the Roland Pettit Centennial Chair in the Department of Chemistry. [4] [5]

She was the president of the American Society for Mass Spectrometry for the period of 2014–2016. [6]

Research

Brodbelt's research centers on the development of mass spectrometry-based methods to characterize organic molecules. [7] [8] Brodbelt's early research established methods to use chemical ionization in ion trap mass spectrometers [9] [10] and applied this method to the analysis of petroleum samples. [11] She subsequently worked on gas-phase ion chemistry [12] and photodissociation [13] as methods to break apart organic compounds before they are analyzed by a mass spectrometer. Her work has led to the analysis of compounds including recreational drugs, [14] [15] sunscreen, [16] and pesticides. [17] Her work also includes investigations into proteins [18] and other organic compounds produced by bacteria including [19] [20]

Selected publications

Awards

She was awarded the Agnes Fay Morgan Research Award in 1995. [21] In 2019, Brodbelt received the Frank H. Field and Joe L. Franklin Award for Outstanding Achievement in Mass Spectrometry from the American Chemical Society. [22] In 2023, she was named one of the "Mentors and Educators" in the Power List by the Analytical Scientist. [23]

Related Research Articles

<span class="mw-page-title-main">Mass spectrometry</span> Analytical technique based on determining mass to charge ratio of ions

Mass spectrometry (MS) is an analytical technique that is used to measure the mass-to-charge ratio of ions. The results are presented as a mass spectrum, a plot of intensity as a function of the mass-to-charge ratio. Mass spectrometry is used in many different fields and is applied to pure samples as well as complex mixtures.

<span class="mw-page-title-main">Tandem mass spectrometry</span> Type of mass spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is a technique in instrumental analysis where two or more stages of analysis using one or more mass analyzer are performed with an additional reaction step in between these analyses to increase their abilities to analyse chemical samples. A common use of tandem MS is the analysis of biomolecules, such as proteins and peptides.

<span class="mw-page-title-main">Gas chromatography–mass spectrometry</span> Analytical method

Gas chromatography–mass spectrometry (GC–MS) is an analytical method that combines the features of gas-chromatography and mass spectrometry to identify different substances within a test sample. Applications of GC–MS include drug detection, fire investigation, environmental analysis, explosives investigation, food and flavor analysis, and identification of unknown samples, including that of material samples obtained from planet Mars during probe missions as early as the 1970s. GC–MS can also be used in airport security to detect substances in luggage or on human beings. Additionally, it can identify trace elements in materials that were previously thought to have disintegrated beyond identification. Like liquid chromatography–mass spectrometry, it allows analysis and detection even of tiny amounts of a substance.

<span class="mw-page-title-main">Electron-capture dissociation</span> Method in mass spectrometry

Electron-capture dissociation (ECD) is a method of fragmenting gas-phase ions for structure elucidation of peptides and proteins in tandem mass spectrometry. It is one of the most widely used techniques for activation and dissociation of mass selected precursor ion in MS/MS. It involves the direct introduction of low-energy electrons to trapped gas-phase ions.

<span class="mw-page-title-main">Ion mobility spectrometry</span> Analytical technique used to separate and identify ionized molecules in the gas phase

Ion mobility spectrometry (IMS) It is a method of conducting analytical research that separates and identifies ionized molecules present in the gas phase based on the mobility of the molecules in a carrier buffer gas. Even though it is used extensively for military or security objectives, such as detecting drugs and explosives, the technology also has many applications in laboratory analysis, including studying small and big biomolecules. IMS instruments are extremely sensitive stand-alone devices, but are often coupled with mass spectrometry, gas chromatography or high-performance liquid chromatography in order to achieve a multi-dimensional separation. They come in various sizes, ranging from a few millimeters to several meters depending on the specific application, and are capable of operating under a broad range of conditions. IMS instruments such as microscale high-field asymmetric-waveform ion mobility spectrometry can be palm-portable for use in a range of applications including volatile organic compound (VOC) monitoring, biological sample analysis, medical diagnosis and food quality monitoring. Systems operated at higher pressure are often accompanied by elevated temperature, while lower pressure systems (1–20 hPa) do not require heating.

<span class="mw-page-title-main">Atmospheric-pressure chemical ionization</span> Ionization method

Atmospheric pressure chemical ionization (APCI) is an ionization method used in mass spectrometry which utilizes gas-phase ion-molecule reactions at atmospheric pressure (105 Pa), commonly coupled with high-performance liquid chromatography (HPLC). APCI is a soft ionization method similar to chemical ionization where primary ions are produced on a solvent spray. The main usage of APCI is for polar and relatively less polar thermally stable compounds with molecular weight less than 1500 Da. The application of APCI with HPLC has gained a large popularity in trace analysis detection such as steroids, pesticides and also in pharmacology for drug metabolites.

Solid phase microextraction, or SPME, is a solid phase extraction sampling technique that involves the use of a fiber coated with an extracting phase, that can be a liquid (polymer) or a solid (sorbent), which extracts different kinds of analytes from different kinds of media, that can be in liquid or gas phase. The quantity of analyte extracted by the fibre is proportional to its concentration in the sample as long as equilibrium is reached or, in case of short time pre-equilibrium, with help of convection or agitation.

<span class="mw-page-title-main">Thermospray</span>

Thermospray is a soft ionization source by which a solvent flow of liquid sample passes through a very thin heated column to become a spray of fine liquid droplets. As a form of atmospheric pressure ionization in mass spectrometry these droplets are then ionized via a low-current discharge electrode to create a solvent ion plasma. A repeller then directs these charged particles through the skimmer and acceleration region to introduce the aerosolized sample to a mass spectrometer. It is particularly useful in liquid chromatography-mass spectrometry (LC-MS).

<span class="mw-page-title-main">Electron-transfer dissociation</span>

Electron-transfer dissociation (ETD) is a method of fragmenting multiply-charged gaseous macromolecules in a mass spectrometer between the stages of tandem mass spectrometry (MS/MS). Similar to electron-capture dissociation, ETD induces fragmentation of large, multiply-charged cations by transferring electrons to them. ETD is used extensively with polymers and biological molecules such as proteins and peptides for sequence analysis. Transferring an electron causes peptide backbone cleavage into c- and z-ions while leaving labile post translational modifications (PTM) intact. The technique only works well for higher charge state peptide or polymer ions (z>2). However, relative to collision-induced dissociation (CID), ETD is advantageous for the fragmentation of longer peptides or even entire proteins. This makes the technique important for top-down proteomics. The method was developed by Hunt and coworkers at the University of Virginia.

Sample preparation for mass spectrometry is used for the optimization of a sample for analysis in a mass spectrometer (MS). Each ionization method has certain factors that must be considered for that method to be successful, such as volume, concentration, sample phase, and composition of the analyte solution. Quite possibly the most important consideration in sample preparation is knowing what phase the sample must be in for analysis to be successful. In some cases the analyte itself must be purified before entering the ion source. In other situations, the matrix, or everything in the solution surrounding the analyte, is the most important factor to consider and adjust. Often, sample preparation itself for mass spectrometry can be avoided by coupling mass spectrometry to a chromatography method, or some other form of separation before entering the mass spectrometer. In some cases, the analyte itself must be adjusted so that analysis is possible, such as in protein mass spectrometry, where usually the protein of interest is cleaved into peptides before analysis, either by in-gel digestion or by proteolysis in solution.

Robert Graham Cooks is the Henry Bohn Hass Distinguished Professor of Chemistry in the Aston Laboratories for Mass Spectrometry at Purdue University. He is an ISI Highly Cited Chemist, with over 1,000 publications and an H-index of 144.

<span class="mw-page-title-main">Christie G. Enke</span> American chemist

Christie G. Enke is a United States academic chemist who made pioneering contributions to the field of analytical chemistry.

<span class="mw-page-title-main">Ion-mobility spectrometry–mass spectrometry</span>

Ion mobility spectrometry–mass spectrometry (IMS-MS) is an analytical chemistry method that separates gas phase ions based on their interaction with a collision gas and their masses. In the first step, the ions are separated according to their mobility through a buffer gas on a millisecond timescale using an ion mobility spectrometer. The separated ions are then introduced into a mass analyzer in a second step where their mass-to-charge ratios can be determined on a microsecond timescale. The effective separation of analytes achieved with this method makes it widely applicable in the analysis of complex samples such as in proteomics and metabolomics.

<span class="mw-page-title-main">Ambient ionization</span>

Ambient ionization is a form of ionization in which ions are formed in an ion source outside the mass spectrometer without sample preparation or separation. Ions can be formed by extraction into charged electrospray droplets, thermally desorbed and ionized by chemical ionization, or laser desorbed or ablated and post-ionized before they enter the mass spectrometer.

<span class="mw-page-title-main">Triple quadrupole mass spectrometer</span> Type of mass spectrometer

A triple quadrupole mass spectrometer (TQMS), is a tandem mass spectrometer consisting of two quadrupole mass analyzers in series, with a (non-mass-resolving) radio frequency (RF)–only quadrupole between them to act as a cell for collision-induced dissociation. This configuration is often abbreviated QqQ, here Q1q2Q3.

<span class="mw-page-title-main">Collision-induced dissociation</span> Mass spectrometry technique to induce fragmentation of selected ions in the gas phase

Collision-induced dissociation (CID), also known as collisionally activated dissociation (CAD), is a mass spectrometry technique to induce fragmentation of selected ions in the gas phase. The selected ions are usually accelerated by applying an electrical potential to increase the ion kinetic energy and then allowed to collide with neutral molecules. In the collision, some of the kinetic energy is converted into internal energy which results in bond breakage and the fragmentation of the molecular ion into smaller fragments. These fragment ions can then be analyzed by tandem mass spectrometry.

<span class="mw-page-title-main">Atmospheric-pressure photoionization</span> Soft ionization method

Atmospheric pressure photoionization (APPI) is a soft ionization method used in mass spectrometry (MS) usually coupled to liquid chromatography (LC). Molecules are ionized using a vacuum ultraviolet (VUV) light source operating at atmospheric pressure, either by direct absorption followed by electron ejection or through ionization of a dopant molecule that leads to chemical ionization of target molecules. The sample is usually a solvent spray that is vaporized by nebulization and heat. The benefit of APPI is that it ionizes molecules across a broad range of polarity and is particularly useful for ionization of low polarity molecules for which other popular ionization methods such as electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) are less suitable. It is also less prone to ion suppression and matrix effects compared to ESI and APCI and typically has a wide linear dynamic range. The application of APPI with LC/MS is commonly used for analysis of petroleum compounds, pesticides, steroids, and drug metabolites lacking polar functional groups and is being extensively deployed for ambient ionization particularly for explosives detection in security applications.

<span class="mw-page-title-main">Laser diode thermal desorption</span>

Laser diode thermal desorption (LDTD) is an ionization technique that is coupled to mass spectrometry to analyze samples with atmospheric pressure chemical ionization (APCI). It uses a laser to thermally desorb analytes that are deposited on a stainless steel sheet sample holder, called LazWell. The coupling of LDTD and APCI is considered to be a soft-ionization technique. With LDTD-APCI, it is possible to analyze samples in forensics, pharmaceuticals, environment, food and clinical studies. LDTD is suitable for small molecules between 0 and 1200 Da and some peptides such as cyclosporine.

Barbara Seliger Larsen is a mass spectrometrist, with a career in instrumentations and applications of mass spectrometry in industry, and served on the board of the American Society for Mass Spectrometry for several terms.

References

  1. ↑ Brodbelt-Lustig, Jennifer Sue (1998). Aspects of ion chemistry in mass spectrometry (Ph.D.). Retrieved October 6, 2021.
  2. ↑ Brodbelt, Jennifer. "Postdoctoral Research Fellowship in Chemistry".{{cite journal}}: Cite journal requires |journal= (help)
  3. ↑ "Jennifer S. Brodbelt - Professor of Biochemistry and Molecular Genetics in Austin, Texas, United States Of America | eMedEvents". eMedEvents.com. Retrieved 2021-10-06.
  4. ↑ "Faculty". utexas.edu. Retrieved December 13, 2016.
  5. ↑ "Jennifer Brodbelt". utexas.edu. Retrieved December 13, 2016.
  6. ↑ "Past Presidents" . Retrieved 2019-04-19.
  7. ↑ Brodbelt, Jennifer S. (1997). "Analytical applications of ion-molecule reactions". Mass Spectrometry Reviews. 16 (2): 91–110. Bibcode:1997MSRv...16...91B. doi:10.1002/(SICI)1098-2787(1997)16:2<91::AID-MAS3>3.0.CO;2-4. ISSN   1098-2787.
  8. ↑ "Jennifer Brodbelt". The Analytical Scientist. 2019. Retrieved 2021-10-06.
  9. ↑ Brodbelt, Jennifer S.; Louris, John N.; Cooks, R. Graham. (1987-05-01). "Chemical ionization in an ion trap mass spectrometer". Analytical Chemistry. 59 (9): 1278–1285. doi:10.1021/ac00136a007. ISSN   0003-2700.
  10. ↑ Brodbelt, Jennifer.; Liou, Chien Chung.; Donovan, Tracy. (1991-07-01). "Selective adduct formation by dimethyl ether chemical ionization in a quadrupole ion trap mass spectrometer and a conventional ion source". Analytical Chemistry. 63 (13): 1205–1209. doi:10.1021/ac00013a005. ISSN   0003-2700.
  11. ↑ Brodbelt, Jennifer S.; Cooks, R. Graham; Wood, Karl V.; Jackson, Thomas J. (1986-01-01). "The Analysis of Metalloporphyrins in Petroleum Using Tandem Mass Spectrometry". Fuel Science and Technology International. 4 (6): 683–698. Bibcode:1986FSTI....4..683B. doi:10.1080/08843758608915836. ISSN   0884-3759.
  12. ↑ Brodbelt, J. S.; Liou, C.-C. (1993-01-01). "New frontiers in host-guest chemistry: The gas phase". Pure and Applied Chemistry (in German). 65 (3): 409–414. doi: 10.1351/pac199365030409 . ISSN   1365-3075. S2CID   31322707.
  13. ↑ Brodbelt, Jennifer S.; Wilson, Jeffrey J. (2009). "Infrared multiphoton dissociation in quadrupole ion traps". Mass Spectrometry Reviews. 28 (3): 390–424. Bibcode:2009MSRv...28..390B. doi:10.1002/mas.20216. ISSN   1098-2787. PMID   19294735.
  14. ↑ Hall, Brad J; Brodbelt, Jennifer S (1997-08-15). "Determination of barbiturates by solid-phase microextraction (SPME) and ion trap gas chromatography–mass spectrometry". Journal of Chromatography A. 777 (2): 275–282. doi:10.1016/S0021-9673(97)00363-4. ISSN   0021-9673. PMID   9299725.
  15. ↑ Hall, Brad J.; Satterfield-Doerr, Mary; Parikh, Aashish R.; Brodbelt, Jennifer S. (1998-05-01). "Determination of Cannabinoids in Water and Human Saliva by Solid-Phase Microextraction and Quadrupole Ion Trap Gas Chromatography/Mass Spectrometry". Analytical Chemistry. 70 (9): 1788–1796. doi:10.1021/ac971228g. ISSN   0003-2700. PMID   9599579.
  16. ↑ Felix, Tonya; Hall, Brad J; S. Brodbelt, Jennifer (1998-10-05). "Determination of benzophenone-3 and metabolites in water and human urine by solid-phase microextraction and quadrupole ion trap GC–MS". Analytica Chimica Acta. 371 (2): 195–203. Bibcode:1998AcAC..371..195F. doi:10.1016/S0003-2670(98)00293-1. ISSN   0003-2670.
  17. ↑ Reyzer, Michelle L; Brodbelt, Jennifer S (2001-06-01). "Analysis of fire ant pesticides in water by solid-phase microextraction and gas chromatography/mass spectrometry or high-performance liquid chromatography/mass spectrometry". Analytica Chimica Acta. 436 (1): 11–20. Bibcode:2001AcAC..436...11R. doi:10.1016/S0003-2670(01)00893-5. ISSN   0003-2670.
  18. ↑ Shaw, Jared B.; Li, Wenzong; Holden, Dustin D.; Zhang, Yan; Griep-Raming, Jens; Fellers, Ryan T.; Early, Bryan P.; Thomas, Paul M.; Kelleher, Neil L.; Brodbelt, Jennifer S. (2013-08-28). "Complete Protein Characterization Using Top-Down Mass Spectrometry and Ultraviolet Photodissociation". Journal of the American Chemical Society. 135 (34): 12646–12651. doi:10.1021/ja4029654. ISSN   0002-7863. PMC   3757099 . PMID   23697802.
  19. ↑ Hankins, Jessica V.; Madsen, James A.; Giles, David K.; Brodbelt, Jennifer S.; Trent, M. Stephen (2012-05-29). "Amino acid addition to Vibrio cholerae LPS establishes a link between surface remodeling in Gram-positive and Gram-negative bacteria". Proceedings of the National Academy of Sciences. 109 (22): 8722–8727. Bibcode:2012PNAS..109.8722H. doi: 10.1073/pnas.1201313109 . ISSN   0027-8424. PMC   3365186 . PMID   22589301.
  20. ↑ Han, Sang-Wook; Lee, Sang-Won; Bahar, Ofir; Schwessinger, Benjamin; Robinson, Michelle R.; Shaw, Jared B.; Madsen, James A.; Brodbelt, Jennifer S.; Ronald, Pamela C. (2012). "Tyrosine sulfation in a Gram-negative bacterium". Nature Communications. 3 (1): 1153. Bibcode:2012NatCo...3.1153H. doi:10.1038/ncomms2157. ISSN   2041-1723. PMC   4305400 . PMID   23093190.
  21. ↑ "Past Award Winners | Iota Sigma Pi". 2021-10-06. Archived from the original on 2021-10-06. Retrieved 2021-10-06.
  22. ↑ "Frank H. Field & Joe L. Franklin Award for Outstanding Achievement in Mass Spectrometry: Vicki H. Wysocki". C&EN Global Enterprise. 95 (1): 49–50. 2017-01-02. doi: 10.1021/cen-09501-awards054 . ISSN   2474-7408.
  23. ↑ "The Power List 2023". The Analytical Scientist. 2023-09-10. Retrieved 2023-09-02.
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