| Organization | Year founded | Focus | Status | 
|---|
| Accord Alliance | 2008 | Healthcare | Active | 
| ACT UP | 1987 | HIV/AIDS | Active | 
| Advocates for Informed Choice | 2006 | Intersex | Renamed interACT | 
| Affirmation: LGBTQ Mormons, Families & Friends | 1977 | Mormons | Active | 
| Against Equality | 2009 | Anti-capitalist discourse |  | 
| American Foundation for Equal Rights | 2009 | Prop. 8 opposition | Active | 
| American Veterans for Equal Rights | 1990 | Veterans | Active | 
| American Military Partner Association | 2009 | LGBTQ military families and Veterans | Merged with OutServe-SLDN and renamed to Modern Military Association of America in 2019 | 
| Aromantic-spectrum Union for Recognition, Education, and Advocacy | 2019 | Aromanticism | Active | 
| Asexual Visibility and Education Network | 2001 | Asexuality | Active | 
| Athlete Ally | 2011 | Sports | Active | 
| Atticus Circle | 2004 | Straight allies | Active | 
| Bash Back! | 2007 | Anarchist direct action | Dissolved | 
| Bialogue | 2005 | Bisexuality |  | 
| BiNet USA | 1990 | Bisexuality | Active | 
| Bisexual Resource Center | 1985 | Bisexuality | Active | 
| Campus Pride | 2001 | Students | Active | 
| Cheer, Dorothy, Cheer! | 2002 | Awareness |  | 
| COLAGE | 1990 | Children of LGBTQ parents | Active | 
| Daughters of Bilitis | 1955 | Lesbian | Dissolved | 
| El/La Para TransLatinas | 2006 | Latinas who are trans women | Active | 
| Empowering Spirits Foundation | 2008 | Community service | Dissolved | 
| Equality Across America | 2009 | National Equality March | Dissolved | 
| Equality Federation | 1997 |  | Active | 
| Family Equality Council | 1979 |  |  | 
| Fight OUT Loud | 2007 |  |  | 
| Fine By Me | 2003 |  | Merged into  Atticus Circle | 
| Freedom to Marry | 2003 | Marriage equality | Dissolved | 
| Gay, Lesbian and Straight Education Network (GLSEN) | 1990 |  |  | 
| Gay Liberation Front#United States (GLF) | 1969 |  | Dissolved | 
| Gay Liberation Network | 1998 | Direct action | Active | 
| Gay Rights National Lobby | 1976 |  | Merged into the HRC | 
| GenderPAC (GPAC) | 1995 |  | Dissolved | 
| GetEQUAL | 2010 |  | Active | 
| GLAAD | 1985 |  | Active | 
| GLBTQ Legal Advocates & Defenders (GLAD) | 1978 |  |  | 
| GLIFAA | 1992 |  | Active | 
| GLMA: Health Professionals Advancing LGBTQ Equality | 1994 (1981)
 | Homophobia in medicine | Active | 
| Global Equality Fund | 2011 |  |  | 
| GSA Network | 1998 |  | Active | 
| Human Rights Campaign (HRC) | 1980 |  | Active | 
| Immigration Equality | 1994 |  | Active | 
| interACT | 2006 |  | Active | 
| Integrity USA | 1974 |  |  | 
| International Foundation for Gender Education (IFGE) | 1987 |  |  | 
| Intersex Campaign for Equality (IC4E) formerly OII-USA | 2011 |  |  | 
| Join the Impact | 2008 |  |  | 
| Keshet | 2001 |  | Active | 
| Lambda Legal | 1971 |  | Active | 
| Lavender Menace | 1970 |  |  | 
| Lesbian and Gay Band Association | 1982 |  |  | 
| Lesbian Avengers | 1992 |  |  | 
| LGBTQ Victory Fund | 1991 |  |  | 
| Love Makes a Family | 1992 |  | Dissolved | 
| LPAC | 2012 |  | Active | 
| Marriage Equality USA | 1998 |  | Dissolved | 
| Mattachine Society | 1950 |  |  | 
| Matthew Shepard Foundation | 1998 |  | Active | 
| Modern Military Association of America | 2012 | LGBTQ military, military families, and veterans | Active—Renamed from OutServe-SLDN with the merger of American Military Partner Association | 
| National Black Justice Collective (NBJC) | 2003 |  | Active | 
| National Center for Lesbian Rights (NCLR) | 1977 |  | Active | 
| National Center for Transgender Equality (NCTE) | 2003 |  | Active | 
| National Coalition of Black Lesbians and Gays | 1978 |  | Dissolved | 
| National Gay Pilots Association (NGPA) | 1996 |  | Active | 
| National LGBT Chamber of Commerce (NGLCC) | 2002 |  | Active | 
| National Lesbian and Gay Journalists Association (NLGJA) | 1990 |  | Active | 
| National LGBTQ Task Force (The Task Force) | 1973 |  | Active | 
| National Transgender Advocacy Coalition | 1999 |  |  | 
| NOH8 Campaign | 2009 | Prop. 8 |  | 
| North American Conference of Homophile Organizations (NACHO) | 1966 |  | Dissolved in 1970 | 
| ONE National Gay & Lesbian Archives | 1952 |  |  | 
| Out & Equal | 1996 |  | Active | 
| OutServe-SLDN | 2012 | LGBTQ military and Veterans | Merged with American Military Partner Association and renamed Modern Military Association of America in 2019 | 
| PFLAG | 1973 |  | Active | 
| Pride at Work | 1994 |  | Active | 
| Queer Nation | 1990 |  |  | 
| RESYST | 2000 |  | Dissolved | 
| Rainbow Railroad USA | 2015 | Global LGBTQI+ refugee support and advocacy | Active | 
| Rainbow Sash | 1998 |  |  | 
| Services & Advocacy for GLBT Elders (SAGE) | 1979 |  | Active | 
| Servicemembers Legal Defense Network (SLDN) | 1993 |  | Merged into OutServe-SLDN | 
| Society for Human Rights | 1924 |  | Dissolved | 
| Soulforce | 1998 |  |  | 
| Sports Equality Foundation | 2023 | LGBTQ Representation in Sport & the LGBTQ Sports Hall of Fame | Active | 
| StartOut | 2009 |  | Active | 
| Sylvia Rivera Law Project | 2002 | Legal aid | Active | 
| The Ace and Aro Advocacy Project (TAAAP) | 2018 | Aromanticism Asexuality
 | Active | 
| Think Again | 1997 |  | Dissolved | 
| Trans Student Educational Resources | 2011 |  | Active | 
| Transgender Law Center | 2002 |  | Active | 
| Truth Wins Out | 2006 |  | Active |