| Name | Year entered | Year exited |
|---|
| 865 Alliance | 2023 | present |
| AC Crusaders | 2011 | 2012 |
| Academica SC | 2018 | 2022 |
| AFC Ann Arbor | 2016 | 2019 |
| AFC Cleveland | 2012 | 2017 |
| AFC Mobile | 2020 | 2022 |
| Akron City FC | 2021 | 2024 |
| Alabama Spirit (Provisional) | 2008 | 2008 |
| Alexandria Reds | 2022 | present |
| Ambassadors FC Ohio | 2025 | present |
| American Soccer Club New York | 2025 | present |
| Annapolis Blues FC | 2023 | 2024 |
| Appalachian FC | 2021 | present |
| Apotheos FC | 2022 | 2024 |
| Arkansas Wolves FC | 2021 | present |
| A.S. Los Angeles | 2019 | 2020 |
| Albion SC Pros/ASC San Diego | 2016 | 2021 |
| Asheville City SC | 2016 | 2019 |
| Albuquerque Asylum | 2004 | 2008 |
| Arizona Sahuaros | 2003 | 2008 |
| ASA Charge | 2014 | 2016 |
| Atlanta FC/Atlanta Silverbacks Reserves/Atlanta Silverbacks FC/Atlanta SC | 2008 | 2019 |
| Atlantic City Diablos | 2007 | 2008 |
| Atlantic City FC | 2018 | 2024 |
| Austin United FC | 2020 | 2024 |
| Bay Area Ambassadors | 2009 | 2012 |
| BCS Clash | 2014 | 2014 |
| Beaches FC/Palm Beach United | 2017 | 2018 |
| Birmingham Hammers | 2016 | 2017 |
| Boca Raton FC | 2017 | 2021 |
| Boston Aztec | 2007 | 2009 |
| Boston City FC | 2016 | 2021 |
| Boston Tea Men | 2010 | 2010 |
| Brooklyn Italians | 2010 | 2019 |
| Buffalo City FC | 2009 | 2009 |
| Buffalo Stallions | 2025 | present |
| Burlington United FC | 2025 | present |
| Buxmont Torch FC/Torch FC | 2011 | present |
| California Odyssey SC | 2023 | present |
| Cape Coral Hurricanes | 2013 | 2014 |
| Carolina RailHawks U23s/Carolina RailHawks NPSL | 2014 | 2016 |
| Carpathia FC | 2020 | present |
| CASL Elite | 2013 | 2013 |
| CD Aguiluchos USA | 2013 | 2018 |
| Cedar Stars FC | 2022 | 2022 |
| Central Florida Panthers SC | 2019 | 2022 |
| Charlottesville Alliance FC | 2018 | 2020 |
| Charlottetowne Hops FC | 2023 | present |
| Chattanooga FC | 2009 | 2019 |
| Chesterfield United FC | 2013 | 2013 |
| Chicago Mustangs (Provisional) | 2016 | 2016 |
| Chico Rooks | 2003 | 2006 |
| Club Atletico Saint Louis | 2022 | 2024 |
| Cincinnati Saints/Dayton Dynamo | 2014 | 2017 |
| City of Angels FC | 2017 | 2019 |
| Cleveland SC | 2018 | present |
| Colorado Crimson | 2007 | 2007 |
| Columbus United | 2024 | 2024 |
| Contra Costa FC | 2021 | 2022 |
| Corinthians FC of San Antonio | 2014 | present |
| Coyotes FC/ Central Texas Coyotes FC | 2020 | present |
| Crossfire Redmond, Crossfire Red, Crossfire White | 2019 | present |
| Cruizers FC | 2025 | present |
| Dakota Fusion FC | 2017 | 2024 |
| Dallas City FC | 2014 | 2021 |
| D.C. United U-23 | 2013 | 2014 |
| Del Rey City SC | 2014 | 2014 |
| Denton Diablos FC | 2019 | 2024 |
| Denver Kickers | 2006 | 2007 |
| Iowa Demon Hawks | 2023 | present |
| Club de Lyon FC | 2025 | present |
| Deportivo Coras USA/Club Xolos USA U-23 | 2015 | 2020 |
| Demize NPSL | 2014 | 2024 |
| Detroit Arsenal | 2005 | 2006 |
| Detroit City FC | 2012 | 2019 |
| District Elite FC | 2025 | present |
| Duluth FC | 2017 | present |
| Dutch Lions FC/Miami Dutch Lions FC | 2016 | present |
| Eau Claire Aris/LaCrosse Aris FC | 2009 | present |
| Ehtar Belleville FC | 2023 | present |
| El Farolito Soccer Club | 2018 | present |
| Electric City Shock SC | 2014 | present |
| Elm City Express | 2017 | 2018 |
| Erie Admirals SC/Erie Commodores FC | 2009 | present |
| FC Arizona | 2017 | 2024 |
| FCAZ Tucson | 2024 | 2024 |
| FC Baltimore/FC Baltimore Christos | 2018 | 2021 |
| FC Bordo Saint Louis | 2014 | present |
| FC Brownsville/ Brownsville NPSL | 2018 | present |
| FC Buffalo | 2010 | 2022 |
| FC Carolina Discoveries/FC Carolina United | 2014 | 2017 |
| FC Columbus | 2018 | present |
| FC Davis | 2018 | present |
| FC Florida | 2025 | present |
| FC Frederick | 2015 | present |
| FC Golden State | 2018 | 2021 |
| FC Hasental | 2011 | 2015 |
| F.C. Indiana | 2007 | 2021 |
| FC Force | 2014 | 2015 |
| FC Monmouth | 2018 | 2024 |
| F.C. New York | 2012 | 2012 |
| FC Pride Elite | 2025 | present |
| FC Sparta Michigan/Michigan Stars FC | 2013 | 2019 |
| Michigan Rangers FC | 2023 | present |
| FC Reading Revolution | 2009 | 2013 |
| FC Tacoma 253/OSA FC/OSA Seattle FC | 2015 | present |
| FC Tulsa | 2010 | 2010 |
| FC Wichita | 2015 | 2019 |
| First State FC | 2020 | 2024 |
| Florida Roots Futbol Club | 2021 | present |
| Fort Lauderdale Strikers U-23 | 2016 | 2016 |
| Fort Pitt Regiment | 2014 | 2018 |
| Fort Wayne FC | 2020 | 2020 |
| Fort Worth Vaqueros FC | 2014 | present |
| Fullerton Rangers | 2012 | 2012 |
| Gate City FC | 2014 | 2014 |
| Georgia Revolution FC | 2011 | present |
| Georgia Storm FC | 2021 | present |
| Glendale Lions FC | 2024 | 2024 |
| Gorge FC | 2014 | 2014 |
| Grand Rapids Alliance | 2005 | 2006 |
| Grand Rapids FC | 2016 | 2019 |
| Greater Binghamton FC | 2012 | 2014 |
| Greater Lowell United FC | 2014 | 2016 |
| Greater Lowell NPSL FC | 2017 | 2018 |
| Greater Lowell Rough Diamonds | 2019 | 2021 |
| Greenville FC | 2018 | 2019 |
| Greenville United FC | 2023 | present |
| Grove Soccer United/ Grove United | 2022 | present |
| Gulf Coast Texans/Pensacola FC | 2013 | present |
| Hartford City FC | 2017 | present |
| Hershey FC | 2013 | present |
| High Desert Elite FC | 2019 | 2020 |
| Hollywood United Hitmen | 2011 | 2011 |
| Houston Hurricanes FC | 2013 | 2013 |
| Idaho Wolves | 2004 | 2004 |
| Indianapolis Braves | 2007 | 2007 |
| Indios USA | 2007 | 2008 |
| Inter Nashville FC | 2017 | 2020 |
| Inter United FC | 2014 | 2014 |
| Iowa Raptors FC | 2023 | present |
| Irving FC/ Gallos FC | 2021 | 2023 |
| Jackson Lions FC | 2022 | present |
| Jacksonville Armada FC | 2018 | 2018 |
| Jacksonville United FC/Jacksonville Armada U-23 | 2011 | present |
| Jersey City Eagles/Clarkstown SC Eagles/FC Motown | 2012 | present |
| Joplin Demize/Demize NPSL | 2014 | present |
| Joy St. Louis Park | 2021 | present |
| Junior Lone Star FC/Philadelphia Lone Star FC | 2012 | 2022 |
| Philadelphia Union Development Squad | 2023 | present |
| Kalamazoo FC | 2016 | 2020 |
| Kansas City Sol | 2023 | 2024 |
| Katy 1895 FC/CF10 Houston FC | 2018 | 2024 |
| Kingston Stockade FC | 2016 | 2024 |
| Kitsap Pumas | 2017 | 2018 |
| Knoxville Force/Emerald Force SC | 2011 | 2018 |
| Kraze United | 2015 | 2017 |
| Lancaster Inferno | 2008 | 2008 |
| Lancaster Rattlers/FC Santa Clarita | 2011 | 2013 |
| Lansing United | 2014 | 2017 |
| Laredo Heat | 2018 | 2024 |
| Las Vegas Knights FC | 2024 | 2024 |
| Las Vegas Legends | 2020 | 2024 |
| Las Vegas Stallions | 2013 | 2013 |
| Las Vegas Strikers | 2003 | 2006 |
| Legacy 76 | 2014 | 2018 |
| Little Rock Rangers | 2016 | 2020 |
| Liverpool Warriors | 2013 | 2016 |
| Long Island Academy | 2007 | 2010 |
| LSA Athletico Lanier | 2020 | 2022 |
| Lubbock Matadors | 2022 | present |
| Magia FC | 2022 | 2022 |
| Maine Sting | 2008 | 2009 |
| Maryland Bobcats FC | 2020 | 2020 |
| Maryland United FC/Charm City FC | 2008 | 2009 |
| Mass United FC | 2011 | 2012 |
| Med City FC | 2017 | present |
| Memphis City FC | 2016 | 2017 |
| Metro Louisville FC | 2020 | 2021 |
| Miami Beach CF/ Atletico De Miami Beach | 2022 | present |
| Miami FC 2/ Miami FC | 2018 | 2019 |
| Miami Fusion FC | 2015 | 2017 |
| Miami United FC | 2013 | 2022 |
| Milwaukee Bavarian SC | 2005 | 2013 |
| Milwaukee Torrent | 2016 | present |
| Minnesota Blast/NSC United | 2005 | 2006 |
| Minneapolis City SC | 2017 | 2022 |
| Minnesota Kings | 2010 | 2011 |
| Minnesota Twin Stars | 2005 | 2024 |
| Minnesota United FC Reserves | 2014 | 2015 |
| Mississippi Storm | 2012 | 2013 |
| Morris County Colonials/Jersey Blues FC | 2008 | 2015 |
| Muskegon Risers SC | 2020 | 2022 |
| Myrtle Beach Mutiny | 2012 | 2018 |
| Napa Valley 1839 FC | 2017 | 2024 |
| Naples United FC | 2017 | present |
| Nashville Atlas FC/Nashville FC | 2014 | 2016 |
| Nashville United | 2020 | 2020 |
| New Hampshire Mountaineers | 2010 | 2010 |
| New Haven United FC | 2025 | present |
| New Jersey Blaze | 2010 | 2011 |
| New Jersey Copa FC | 2016 | 2019 |
| New Jersey United AC | 2024 | present |
| New Orleans Jesters | 2013 | present |
| New York Athletic Club S.C. | 2008 | 2020 |
| New York Cosmos B | 2015 | 2019 |
| New York Red Bull NPSL | 2010 | 2014 |
| New York Shockers | 2021 | present |
| NorCal Lamorinda United SC | 2009 | 2009 |
| North Alabama SC | 2020 | 2022 |
| North Coast Tsunami | 2012 | 2012 |
| Northern Virginia United FC | 2018 | present |
| North County Battalion | 2016 | 2016 |
| Northern Nevada Aces | 2003 | 2004 |
| Oakland SC | 2022 | 2024 |
| OKC 1889 FC | 2021 | 2024 |
| Oklahoma City FC | 2014 | 2014 |
| Orange County FC | 2017 | 2019 |
| Orange County Pateadores FC | 2013 | 2013 |
| Osner's FC | 2025 | present |
| Oxnard Guerreros FC | 2017 | 2020 |
| Ozark FC | 2017 | 2020 |
| Panathinaikos Chicago | 2021 | 2022 |
| PDX FC | 2017 | 2021 |
| Pensacola FC | 2020 | 2024 |
| Pennsylvania Classics | 2022 | present |
| Pennsylvania Stoners | 2008 | 2009 |
| Performance FC Phoenix | 2008 | 2008 |
| Players Development Academy | 2025 | present |
| Philadelphia Ukrainian Nationals | 2022 | present |
| Phoenix Banat Storm | 2006 | 2006 |
| Phoenix Monsoon | 2012 | 2012 |
| Pierce County FC | 2017 | 2017 |
| Steel City FC (Pennsylvania)/Pittsburgh Hotspurs | 2019 | 2024 |
| Pocono Snow | 2009 | 2013 |
| Port City FC | 2020 | 2021 |
| PSC FC Florida | 2025 | present |
| Princeton 56ers/Madison 56ers | 2005 | 2015 |
| Project 510 | 2020 | 2020 |
| Pumas FC | 2009 | 2010 |
| Quad City Eagles | 2011 | 2014 |
| Queen City FC | 2007 | 2008 |
| Real San Jose | 2007 | 2025 |
| Real Shore F.C. | 2007 | 2007 |
| Redwood City Ruckus | 2006 | 2006 |
| Regals SCA/Houston Regals | 2013 | 2018 |
| Reign FK | 2021 | 2023 |
| Ristozi FC | 2025 | present |
| Rhode Island Reds F.C. | 2012 | 2020 |
| Rochester River Dogz FC/Rochester Lancers | 2016 | 2021 |
| Rocket City United | 2008 | 2013 |
| Rockford Raptors/ Chicago Fire NPSL/ Indiana Fire/ Indy Eleven NPSL | 2007 | 2016 |
| RVA FC/ Fredericksburg FC | 2013 | 2017 |
| Sacramento Gold | 2010 | present |
| Sacramento Knights | 2004 | 2007 |
| Saint Louis Club Atletico | 2018 | present |
| Salinas Valley Samba | 2004 | 2009 |
| San Diego Boca F.C./ FC Force | 2010 | 2014 |
| San Diego Flash | 2011 | 2015 |
| San Diego Pumitas | 2005 | 2007 |
| San Diego United | 2008 | 2009 |
| San Francisco Stompers FC/ East Bay FC Stompers/Oakland Stompers | 2012 | present |
| San Leandro United FC | 2025 | present |
| San Ramon FC | 2019 | 2024 |
| Santa Ana Winds FC | 2011 | 2011 |
| Santa Cruz County Breakers | 2007 | 2008 |
| Saturn FC | 2009 | 2009 |
| SC Brave Lions/Lions United FC | 2022 | 2024 |
| Seacoast United Mariners | 2012 | 2013 |
| Seacoast United Phantoms | 2011 | 2013 |
| Seattle Sporting FC | 2014 | 2014 |
| Shreveport Rafters FC | 2016 | 2018 |
| Sioux Falls Thunder FC | 2017 | present |
| Sonoma County Sol | 2004 | present |
| SoCal SC | 2016 | 2017 |
| Southern California Fusion | 2006 | 2007 |
| Southern Oregon Starphire FC | 2009 | 2010 |
| Southern States SC | 2021 | present |
| Spartans Futbol Club/FC Mulhouse Portland/International Portland Select FC | 2014 | present |
| Spokane Shadow | 2017 | 2021 |
| Sport Club Corinthians USA | 2016 | 2017 |
| Storm FC | 2014 | 2021 |
| Sunflower State FC | 2017 | 2024 |
| Syracuse FC | 2017 | present |
| Tacoma Stars | 2020 | 2021 |
| Tallahassee SC | 2020 | 2024 |
| Tampa Bay Rowdies 2 | 2016 | 2016 |
| Tampa Marauders | 2013 | 2014 |
| Temecula FC | 2014 | 2021 |
| Tobacco Road FC | 2016 | 2016 |
| Toledo Villa FC | 2019 | 2020 |
| TSC Maryland Red Devils | 2010 | 2010 |
| TSF FC | 2017 | 2018 |
| Tucson Tiburons | 2003 | 2003 |
| Tulsa Athletic | 2013 | 2024 |
| Tyler FC | 2017 | 2019 |
| Upward Stars | 2014 | 2015 |
| Utah Salt Ratz | 2003 | 2004 |
| Valeo FC | 2020 | 2024 |
| Virginia Beach City FC | 2014 | present |
| Virginia Dream FC | 2023 | present |
| VSLT FC | 2017 | 2017 |
| West Chester United SC | 2017 | present |
| Midland-Odessa Sockers FC/West Texas FC | 2017 | present |
| Weston FC | 2015 | 2016 |
| Zanesville Athletic FC | 2013 | 2013 |