Lysine 6-dehydrogenase

Lysine 6-dehydrogenase
Identifiers
EC no.	1.4.1.18
CAS no.	89400-30-6
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Lysine 6-dehydrogenase (EC 1.4.1.18, L-lysine epsilon-dehydrogenase, L-lysine 6-dehydrogenase, LysDH) is an enzyme with systematic name L-lysine:NAD⁺ 6-oxidoreductase (deaminating). ^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

L-lysine + NAD⁺${\displaystyle \rightleftharpoons }$ (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + NADH + H⁺ + NH₃ (overall reaction)

(1a) L-lysine + NAD⁺ + H₂O ${\displaystyle \rightleftharpoons }$ (S)-2-amino-6-oxohexanoate + NADH + H⁺ + NH₃

(1b) (S)-2-amino-6-oxohexanoate ${\displaystyle \rightleftharpoons }$ (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + H₂O (spontaneous)

The enzyme is highly specific for L-lysine as substrate, although S-(2-aminoethyl)-L-cysteine can act as a substrate, but more slowly.

References

1. Misono H, Nagasaki S (April 1982). "Occurrence of L-lysine epsilon-dehydrogenase in Agrobacterium tumefaciens". Journal of Bacteriology. 150 (1): 398–401. doi:10.1128/jb.150.1.398-401.1982. PMC   220128 . PMID   6801024.
2. Misono H, Uehigashi H, Morimoto E, Nagasaki S (1985). "Purification and properties of L-lysine ε-dehydrogenase from Agrobacterium tumefaciens". Agric. Biol. Chem. 49 (7): 2253–2255. doi: 10.1271/bbb1961.49.2253 .
3. Misono H, Hashimoto H, Uehigashi H, Nagata S, Nagasaki S (June 1989). "Properties of L-lysine epsilon-dehydrogenase from Agrobacterium tumefaciens". Journal of Biochemistry. 105 (6): 1002–8. doi: 10.1093/oxfordjournals.jbchem.a122757 . PMID   2768207.
4. Heydari M, Ohshima T, Nunoura-Kominato N, Sakuraba H (February 2004). "Highly stable L-lysine 6-dehydrogenase from the thermophile Geobacillus stearothermophilus isolated from a Japanese hot spring: characterization, gene cloning and sequencing, and expression". Applied and Environmental Microbiology. 70 (2): 937–42. Bibcode:2004ApEnM..70..937H. doi:10.1128/aem.70.2.937-942.2004. PMC   348916 . PMID   14766574.

External links

• Lysine+6-dehydrogenase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)