Middlebrook 7H11 Agar

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Middlebrook 7H11 agar is identical to Middlebrook 7H10 agar, with an addition of pancreatic digest of casein to facilitate the growth of fastidious cultures of M. tuberculosis . [1]

Middlebrook 7H10 Agar is a solid growth medium specially used for culture of Mycobacterium, notably Mycobacterium tuberculosis. It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria.

Casein pronounced "kay-seen" in British English, is a family of related phosphoproteins. These proteins are commonly found in mammalian milk, comprising c. 80% of the proteins in cow's milk and between 20% and 45% of the proteins in human milk. Sheep and buffalo milk have a higher casein content than other types of milk with human milk having a particularly low casein content.

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Mycobactin J may also be added to Middlebrook 7H11 agar to allow the recovery of M. genavense . [2]

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Löwenstein–Jensen medium

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Middlebrook 7H9 broth is a liquid growth medium specially used for culture of Mycobacterium species, notably Mycobacterium tuberculosis.

References

  1. ↑ Cohn, M.L., et al. 1968. Am. Rev. Respir. Dis.; 98:295
  2. ↑ Coyle, M.; et al. (1992). "Laboratory aspects of "Mycobacterium genavense," a proposed species isolated from AIDS patients". Journal of Clinical Microbiology. 30 (12): 3206–3212. PMC   270629  Lock-green.svg. PMID   1280652.
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