Middlebrook 7H9 Broth

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Middlebrook 7H9 broth is a liquid growth medium specially used for culture of Mycobacterium species, notably Mycobacterium tuberculosis . [1]

Contents

Composition


Middlebrook 7H9 broth supports the growth of mycobacterial species when supplemented with nutrients such as glycerol, oleic acid, albumin, and dextrose, except for Mycobacterium bovis , which is inhibited by glycerol. Cultures should be read within 5–7 days after inoculation and once a week thereafter for up to 8 weeks.

Middlebrook broth is commonly used in the preparation of inocula for antimicrobial assays, biochemical tests (arylsufatase and tellurite reduction), and maintenance of stock strains.

Additionally, 7H9 broth is used as a medium in the mycobacteria growth indicator tube. [2]

See also

References

  1. ↑ Atlas, Ronald M.; James W. Snyder (2006). Handbook of media for clinical microbiology. CRC Press. ISBN   978-0-8493-3795-6.
  2. ↑ Siddiqi, Salman H.; Sabine Rüsch-Gerdes (2006). Procedure Manual For BACTEC MGIT 960 TB System.
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