YEPD

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YPD medium bottle and YPD agar plate YPDmedium.jpg
YPD medium bottle and YPD agar plate

YEPD or yeast extract peptone dextrose, also often abbreviated as YPD, is a complete medium for yeast growth. It contains yeast extract, peptone, double-distilled water, and glucose (dextrose). [1] It can be used as solid medium by including agar. The yeast extract will typically contain all the amino acids necessary for growth. By being a complete medium, YEPD cannot be used as a selection medium to test for auxotrophs. Instead, YEPD is used as a growth medium to grow yeast cultures.

YEPD typically contains 1% yeast extract, 2% peptone, and 2% glucose in distilled water. It may be made as a broth, or made into an agar gel by adding 1.5 to 2% agar. [2] [3]

References

  1. "YPD media" . Cold Spring Harbor Protocols. 2010 (9): pdb.rec12315. 2010-09-01. doi:10.1101/pdb.rec12315. ISSN   1940-3402.
  2. "Yeast Extract-Peptone-Dextrose (YPD) Agar" (PDF). Archived (PDF) from the original on 2017-12-29. Retrieved 2017-12-29.
  3. "protocol: YPD plates". fg.cns.utexas.edu. Archived from the original on 2010-03-03. Retrieved 2017-12-29.