Multisite-specific tRNA:(cytosine-C5)-methyltransferase

Multisite-specific tRNA:(cytosine-C5)-methyltransferase
Identifiers
EC no.	2.1.1.202
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Multisite-specific tRNA:(cytosine-C5)-methyltransferase (EC 2.1.1.202, multisite-specific tRNA:m5C-methyltransferase, TRM4 (gene)) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (cytosine-C5)-methyltransferase. ^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

(1) S-adenosyl-L-methionine + cytosine ³⁴ in tRNA precursor ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + 5-methylcytosine ³⁴ in tRNA precursor

(2) S-adenosyl-L-methionine + cytosine⁴⁰ in tRNA precursor ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + 5-methylcytosine⁴⁰ in tRNA precursor

(3) S-adenosyl-L-methionine + cytosine⁴⁸ in tRNA ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + 5-methylcytosine⁴⁸ in tRNA

(4) S-adenosyl-L-methionine + cytosine⁴⁹ in tRNA ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + 5-methylcytosine⁴⁹ in tRNA

The enzyme from Saccharomyces cerevisiae is responsible for complete 5-methylcytosine methylations of yeast tRNA.

References

1. Motorin Y, Grosjean H (August 1999). "Multisite-specific tRNA:m5C-methyltransferase (Trm4) in yeast Saccharomyces cerevisiae: identification of the gene and substrate specificity of the enzyme". RNA. 5 (8): 1105–18. doi:10.1017/s1355838299982201. PMC   1369833 . PMID   10445884.
2. Jiang HQ, Motorin Y, Jin YX, Grosjean H (July 1997). "Pleiotropic effects of intron removal on base modification pattern of yeast tRNAPhe: an in vitro study". Nucleic Acids Research. 25 (14): 2694–701. doi:10.1093/nar/25.14.2694. PMC   146816 . PMID   9207014.
3. Strobel MC, Abelson J (July 1986). "Effect of intron mutations on processing and function of Saccharomyces cerevisiae SUP53 tRNA in vitro and in vivo". Molecular and Cellular Biology. 6 (7): 2663–73. doi:10.1128/mcb.6.7.2663. PMC   367823 . PMID   3537724.
4. Walbott H, Husson C, Auxilien S, Golinelli-Pimpaneau B (July 2007). "Cysteine of sequence motif VI is essential for nucleophilic catalysis by yeast tRNA m5C methyltransferase". RNA. 13 (7): 967–73. doi:10.1261/rna.515707. PMC   1894932 . PMID   17475914.

External links

• Multisite-specific+tRNA:(cytosine-C5)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)