TRNA:m4X modification enzyme

TRNA:m4X modification enzyme
Identifiers
EC no.	2.1.1.225
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

TRNA:m4X modification enzyme (EC 2.1.1.225, TRM13, Trm13p, tRNA:Xm4 modification enzyme) is an enzyme with systematic name S-adenosyl-L-methionine:tRNAPro/His/Gly(GCC) (cytidine/adenosine4-2'-O)-methyltransferase. ^[1] This enzyme catalyses the following chemical reaction

(1) S-adenosyl-L-methionine + cytidine ⁴ in tRNAPro ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + 2'-O-methylcytidine⁴ in tRNAPro

(2) S-adenosyl-L-methionine + cytidine⁴ in tRNAGly(GCC) ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + 2'-O-methylcytidine⁴ in tRNAGly(GCC)

(3) S-adenosyl-L-methionine + adenosine ⁴ in tRNAHis ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + 2'-O-methyladenosine⁴ in tRNAHis

The enzyme from Saccharomyces cerevisiae 2'-O-methylates cytidine ⁴ in tRNAPro and tRNAGly(GCC), and adenosine ⁴ in tRNAHis.

References

1. Wilkinson ML, Crary SM, Jackman JE, Grayhack EJ, Phizicky EM (March 2007). "The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4". RNA. 13 (3): 404–13. doi:10.1261/rna.399607. PMC   1800514 . PMID   17242307.

External links

• TRNA:m4X+modification+enzyme at the U.S. National Library of Medicine Medical Subject Headings (MeSH)