TRNA (pseudouridine54-N1)-methyltransferase

TRNA (pseudouridine54-N1)-methyltransferase
Identifiers
EC no.	2.1.1.257
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

TRNA (pseudouridine⁵⁴-N¹)-methyltransferase (EC 2.1.1.257, TrmY, m1Psi methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (pseudouridine54-N1)-methyltransferase. ^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + pseudouridine ⁵⁴ in tRNA ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + N¹-methylpseudouridine ⁵⁴ in tRNA

This archaeal enzyme is specific for the 54 position.

References

1. Chen HY, Yuan YA (December 2010). "Crystal structure of Mj1640/DUF358 protein reveals a putative SPOUT-class RNA methyltransferase". Journal of Molecular Cell Biology. 2 (6): 366–74. doi: 10.1093/jmcb/mjq034 . PMID   21098051.
2. Wurm JP, Griese M, Bahr U, Held M, Heckel A, Karas M, Soppa J, Wöhnert J (March 2012). "Identification of the enzyme responsible for N1-methylation of pseudouridine 54 in archaeal tRNAs". RNA. 18 (3): 412–20. doi:10.1261/rna.028498.111. PMC   3285930 . PMID   22274954.
3. Chatterjee K, Blaby IK, Thiaville PC, Majumder M, Grosjean H, Yuan YA, Gupta R, de Crécy-Lagard V (March 2012). "The archaeal COG1901/DUF358 SPOUT-methyltransferase members, together with pseudouridine synthase Pus10, catalyze the formation of 1-methylpseudouridine at position 54 of tRNA". RNA. 18 (3): 421–33. doi:10.1261/rna.030841.111. PMC   3285931 . PMID   22274953.

External links

• TRNA+(pseudouridine54-N1)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)