TRNA (cytidine34-2'-O)-methyltransferase

tRNA (cytidine34-2'-O)-methyltransferase
Identifiers
EC no.	2.1.1.207
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

tRNA (cytidine³⁴-2'-O)-methyltransferase (EC 2.1.1.207, yibK (gene), methyltransferase yibK, TrmL, tRNA methyltransferase L, tRNA (cytidine^34/5-carboxymethylaminomethyluridine³⁴-2'-O)-methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (cytidine^34/5-carboxymethylaminomethyluridine³⁴-2'-O)-methyltransferase. ^[1] This enzyme catalyses the following chemical reaction

(1) S-adenosyl-L-methionine + cytidine ³⁴ in tRNA ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + 2'-O-methylcytidine ³⁴ in tRNA

(2) S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine ³⁴ in tRNALeu ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine³⁴ in tRNALeu

The enzyme from Escherichia coli catalyses the 2'-O-methylation of cytidine or 5-carboxymethylaminomethyluridine at the wobble position at nucleotide 34 in tRNALeuCmAA and tRNALeucmnm5UmAA.

References

1. Benítez-Páez A, Villarroya M, Douthwaite S, Gabaldón T, Armengod ME (November 2010). "YibK is the 2'-O-methyltransferase TrmL that modifies the wobble nucleotide in Escherichia coli tRNA(Leu) isoacceptors". RNA. 16 (11): 2131–43. doi:10.1261/rna.2245910. PMC   2957053 . PMID   20855540.

External links

• TRNA+(cytidine34-2'-O)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)