Tricetin 3',4',5'-O-trimethyltransferase

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Tricetin 3',4',5'-O-trimethyltransferase
Identifiers
EC no. 2.1.1.169
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Tricetin 3',4',5'-O-trimethyltransferase (EC 2.1.1.169, FOMT, TaOMT1, TaCOMT1, TaOMT2) is an enzyme with systematic name S-adenosyl-L-methionine:tricetin 3',4',5'-O-trimethyltransferase. [1] [2] [3] This enzyme catalyses the following chemical reaction

3 S-adenosyl-L-methionine + tricetin 3 S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin (overall reaction)
(1a) S-adenosyl-L-methionine + tricetin S-adenosyl-L-homocysteine + 3'-O-methyltricetin
(1b) S-adenosyl-L-methionine + 3'-O-methyltricetin S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin
(1c) S-adenosyl-L-methionine + 3',5'-O-dimethyltricetin S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin

The enzyme from Triticum aestivum catalyses the sequential O-methylation of tricetin via 3'-O-methyltricetin, 3',5'-O-methyltricetin to 3',4',5'-O-trimethyltricetin.

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References

  1. Kornblatt JA, Zhou JM, Ibrahim RK (May 2008). "Structure-activity relationships of wheat flavone O-methyltransferase: a homodimer of convenience". The FEBS Journal. 275 (9): 2255–66. doi: 10.1111/j.1742-4658.2008.06377.x . PMID   18397325.
  2. Zhou JM, Gold ND, Martin VJ, Wollenweber E, Ibrahim RK (July 2006). "Sequential O-methylation of tricetin by a single gene product in wheat". Biochimica et Biophysica Acta (BBA) - General Subjects. 1760 (7): 1115–24. doi:10.1016/j.bbagen.2006.02.008. PMID   16730127.
  3. Zhou JM, Seo YW, Ibrahim RK (April 2009). "Biochemical characterization of a putative wheat caffeic acid O-methyltransferase". Plant Physiology and Biochemistry. 47 (4): 322–6. doi:10.1016/j.plaphy.2008.11.011. PMID   19211254.