UDP-N-acetylmuramoylpentapeptide-lysine N6-alanyltransferase

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UDP-N-acetylmuramoylpentapeptide-lysine N6-alanyltransferase
Identifiers
EC no. 2.3.2.10
CAS no. 37257-26-4
Databases
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BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
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Gene Ontology AmiGO / QuickGO
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In enzymology, an UDP-N-acetylmuramoylpentapeptide-lysine N6-alanyltransferase (EC 2.3.2.10) is an enzyme that catalyzes the chemical reaction

L-alanyl-tRNA + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine tRNA + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D- alanyl-D-alanine

Thus, the two substrates of this enzyme are L-alanyl-tRNA and UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine, whereas its 3 products are tRNA, UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-, and alanyl-D-alanine.

This enzyme belongs to the family of transferases, specifically the aminoacyltransferases. The systematic name of this enzyme class is L-alanyl-tRNA:UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-ala nyl-D-alanine N6-alanyltransferase. Other names in common use include alanyl-transfer ribonucleate-uridine, diphosphoacetylmuramoylpentapeptide transferase, UDP-N-acetylmuramoylpentapeptide lysine N6-alanyltransferase, uridine diphosphoacetylmuramoylpentapeptide lysine, N6-alanyltransferase, L-alanyl-tRNA:UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-, and alanyl-D-alanine 6-N-alanyltransferase. This enzyme participates in peptidoglycan biosynthesis.

Structural studies

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 1P4N, 1XE4, 1XF8, and 1XIX.

Related Research Articles

<span class="mw-page-title-main">DD-transpeptidase</span>

DD-transpeptidase is a bacterial enzyme that catalyzes the transfer of the R-L-αα-D-alanyl moiety of R-L-αα-D-alanyl-D-alanine carbonyl donors to the γ-OH of their active-site serine and from this to a final acceptor. It is involved in bacterial cell wall biosynthesis, namely, the transpeptidation that crosslinks the peptide side chains of peptidoglycan strands.

In enzymology, an alanine—tRNA ligase is an enzyme that catalyzes the chemical reaction

<span class="mw-page-title-main">D-alanine—D-alanine ligase</span> Enzyme belonging to the ligase family

In enzymology, a D-alanine—D-alanine ligase is an enzyme that catalyzes the chemical reaction

In enzymology, a lysine—tRNA ligase is an enzyme that catalyzes the chemical reaction

In enzymology, a UDP-N-acetylmuramate—L-alanine ligase is an enzyme that catalyzes the chemical reaction

In enzymology, a UDP-N-acetylmuramoyl-L-alanine—D-glutamate ligase is an enzyme that catalyzes the chemical reaction

In enzymology, a UDP-N-acetylmuramoyl-L-alanyl-D-glutamate—L-lysine ligase is an enzyme that catalyzes the chemical reaction

In enzymology, a UDP-N-acetylmuramoyl-tripeptide—D-alanyl-D-alanine ligase is an enzyme that catalyzes the chemical reaction

<span class="mw-page-title-main">UDP-N-acetylglucosamine 1-carboxyvinyltransferase</span> Class of enzymes

In enzymology, an UDP-N-acetylglucosamine 1-carboxyvinyltransferase is an enzyme that catalyzes the first committed step in peptidoglycan biosynthesis of bacteria:

In enzymology, a phospho-N-acetylmuramoyl-pentapeptide-transferase is an enzyme that catalyzes the chemical reaction

The bacterial cell wall provides strength and rigidity to counteract internal osmotic pressure, and protection against the environment. The peptidoglycan layer gives the cell wall its strength, and helps maintain the overall shape of the cell. The basic peptidoglycan structure of both Gram-positive and Gram-negative bacteria comprises a sheet of glycan chains connected by short cross-linking polypeptides. Biosynthesis of peptidoglycan is a multi-step process comprising three main stages:

  1. formation of UDP-N-acetylmuramic acid (UDPMurNAc) from N-acetylglucosamine (GlcNAc).
  2. addition of a short polypeptide chain to the UDPMurNAc.
  3. addition of a second GlcNAc to the disaccharide-pentapeptide building block and transport of this unit through the cytoplasmic membrane and incorporation into the growing peptidoglycan layer.

Lipid II:glycine glycyltransferase (EC 2.3.2.16, N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:N6-glycine transferase, femX (gene)) is an enzyme with systematic name alanyl-D-alanine-diphospho-ditrans, octacis-undecaprenyl-N-acetylglucosamine:glycine N6-glycyltransferase. This enzyme catalyses the following chemical reaction

N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase (EC 2.3.2.17, femA (gene)) is an enzyme with systematic name N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-ditrans,octacis-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase. This enzyme catalyses the following chemical reaction

N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-triglycine)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase (EC 2.3.2.18, femB (gene)) is an enzyme with systematic name N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-triglycine)-D-alanyl-D-alanine-ditrans,octacis-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase. This enzyme catalyses the following chemical reaction

FEMB may refer to:

Muramoylpentapeptide carboxypeptidase is an enzyme. This enzyme catalyses the following chemical reaction.

Muramoyltetrapeptide carboxypeptidase is an enzyme. This enzyme catalyses the following chemical reaction

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate—2,6-diaminopimelate ligase is an enzyme with systematic name UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-2,6-diaminoheptanedioate gamma-ligase (ADP-forming). This enzyme catalyses the following chemical reaction

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate—D-lysine ligase is an enzyme with systematic name UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:D-lysine alpha-ligase (ADP-forming). This enzyme catalyses the following chemical reaction

References


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