| 2025 Santa Cruz Challenger | |
|---|---|
| Date | 16–21 June |
| Edition | 6th |
| Surface | Clay |
| Location | Santa Cruz de la Sierra, Bolivia |
| Champions | |
| Singles | |
 Alex Barrena | |
| Doubles | |
| Mariano Kestelboim / Gonzalo Villanueva | |
The 2025 Bolivia Open was a professional tennis tournament played on clay courts. It was the sixth edition of the tournament which was part of the 2025 ATP Challenger Tour. It took place in Santa Cruz de la Sierra, Bolivia between 16 and 21 June 2025.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
 BOL | Murkel Dellien | 220 | 1 |
 BRA | |||
| ARG | Juan Bautista Torres | 283 | 3 |
| ARG | Genaro Alberto Olivieri | 289 | 4 |
| ARG | Renzo Olivo | 304 | 5 |
| BOL | Juan Carlos Prado Ángelo | 310 | 6 |
| ARG | Lautaro Midón | 311 | 7 |
| BRA | João Lucas Reis da Silva | 313 | 8 |
The following players received wildcards into the singles main draw:
Facundo Bagnis Raúl García Federico Zeballos The following player received entry into the singles main draw as an alternate:
Tomás FarjatThe following players received entry from the qualifying draw:
Luis Carlos Álvarez 
Diego Augusto Barreto Sánchez Santiago de la Fuente
Lucas Gerch John Sperle Bautista VilicichThe following player received entry as a lucky loser:
Juan Manuel La Serna Alex Barrena def. 
Franco Roncadelli 5–7, 7–5, 6–3. Mariano Kestelboim / Gonzalo Villanueva def. Boris Arias / Federico Zeballos 6–3, 6–2.